| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,960,659 | T→A | 10.1% | intergenic (‑231/‑98) | mnxG ← / → PP_3491 | manganese‑oxidizing multicopper oxidase/enoly‑coenzyme A hydratase/isomerase family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,960,659 | 0 | T | A | 10.1% | 139.4 / 4.2 | 49 | intergenic (‑231/‑98) | mnxG/PP_3491 | manganese‑oxidizing multicopper oxidase/enoly‑coenzyme A hydratase/isomerase family protein |
| Reads supporting (aligned to +/- strand): ref base T (23/21); new base A (2/3); total (25/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
TACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATACCCCGGCAGCAACAGACCATGTACTGGCCGAG > NC_002947/3960515‑3960807 | tACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCAAAAAAAAAAAAAACGCACGGTACCCTTGCACAATTGTCGAtt > 2:113970/1‑150 (MQ=255) gATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATtgtg < 2:351641/150‑1 (MQ=255) aaCCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAg > 2:146929/1‑150 (MQ=255) aaCCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGACAATTGTGCaat < 2:1156820/150‑2 (MQ=255) cGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCa > 1:83277/1‑150 (MQ=255) cAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCaaa > 1:1324935/1‑150 (MQ=255) gaggaTCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAg < 2:1324935/150‑1 (MQ=255) tCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCaa < 1:1244960/127‑1 (MQ=255) tCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCaa > 2:1244960/1‑127 (MQ=255) cccccAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCt < 1:1204498/150‑1 (MQ=255) cccAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGc > 2:610918/1‑150 (MQ=255) gcagGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCCTGCCAGGATCCTGCGAtc < 1:759749/150‑1 (MQ=255) cccccGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGc < 2:1162866/150‑1 (MQ=255) gggggACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAGTTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGa > 2:972063/1‑150 (MQ=255) ggggACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGAc > 1:228354/1‑150 (MQ=255) ggAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATCGACAATTGTGCAAgg > 1:561043/1‑98 (MQ=39) ggAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATCGACAATTGTGCAAgg < 2:561043/98‑1 (MQ=255) ccGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGaaa > 1:629849/1‑150 (MQ=255) cGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACtt < 2:49058/144‑1 (MQ=255) cGTGGGGGCAAAGGATGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACtt > 1:49058/1‑144 (MQ=255) gCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAGAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATaa < 1:622235/150‑1 (MQ=255) gCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTc > 1:246563/1‑72 (MQ=255) gCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTc < 2:246563/72‑1 (MQ=255) aaaGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACa > 2:1426618/1‑150 (MQ=255) ctgctCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCa < 1:1377418/150‑1 (MQ=255) tgctCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAg > 1:401035/1‑150 (MQ=255) cacgAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCaa < 2:589393/134‑1 (MQ=255) cacaAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCaa > 1:589393/1‑134 (MQ=255) acaAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCa < 2:1305738/103‑1 (MQ=255) acaAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCa > 1:1305738/1‑103 (MQ=255) acaAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGaa > 1:265685/1‑150 (MQ=255) caAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAAc < 2:857276/150‑1 (MQ=255) gcgcgcTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAAt < 1:972063/150‑1 (MQ=255) gcgcgcTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAAt < 2:401035/150‑1 (MQ=255) cTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCacac > 2:926335/1‑150 (MQ=255) tCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAAt > 2:980815/1‑113 (MQ=255) tCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAAt < 1:980815/113‑1 (MQ=255) acaAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCa > 2:631877/1‑150 (MQ=255) gTAAAAACGCACGGTACCCTTGCACAAGTGTCGATTGTGCAAGGCACATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTCCACATAGAAAAGCAATAATCAAGCAGGGCGAACGCAATGACTGAAAACCCGCACAccc > 2:160959/1‑150 (MQ=255) tAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATAcccc > 1:871787/1‑150 (MQ=255) tAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATAcccc > 1:488398/1‑150 (MQ=255) aaaaCGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATACCCCgg < 1:146929/150‑1 (MQ=255) cGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATACCCCGGCAGCAACa < 1:113970/150‑1 (MQ=255) cTTGCACAATCGACAATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCaa < 1:792156/111‑1 (MQ=255) cTTGCACAATCGACAATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCaa > 2:792156/1‑111 (MQ=255) ttGCACAATTGTAGATTGTGCAAGGCAAATTCTCATGCCAGGATCCTGCGATCCTCCAGCTAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATACCCCGGCAGCAACAGACCATGt < 1:160959/150‑1 (MQ=255) gCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCa < 1:645185/96‑1 (MQ=255) gCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCa > 2:645185/1‑96 (MQ=255) gTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATACCCCGGCAGCAACAGACCATGTACTGGCCGAg < 2:488398/150‑1 (MQ=255) | TACGATCAACCGCAACGGATTGGGGAGGATCCCCCAGCAGGTTCCCCCGAATCGGGGGACGGAATCCCGTGGGGGCAAAGGCTGCTCCACAAGCGCGCGCTGGCCTCACACAAGGTAAAAACGCACGGTACCCTTGCACAATTGTCGATTGTGCAAGGCAAATTCGCATGCCAGGATCCTGCGATCCTCCAGCGAACTCCAGACTTCACTTGGAAAATCAATAACAAAGCAGGGAGAACGCAATGACTGCACACGCTCATACCCCGGCAGCAACAGACCATGTACTGGCCGAG > NC_002947/3960515‑3960807 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |