Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,982,882 T→A 7.2% H119Q (CAT→CAA)  PP_3510 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,982,8820TA 7.2% 210.0 / 7.0 70H119Q (CAT→CAA) PP_3510hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (30/34);  new base A (2/3);  total (32/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01

GCAAAGGCACGTATGCTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCAAGACAATGAGGG  >  NC_002947/3982733‑3983026
                                                                                                                                                     |                                                                                                                                                
gCAAAGGCACGTATGCTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCAt                                                                                                                                                  >  2:943061/1‑150 (MQ=255)
           tATGCTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGTCAATGCCCCTGcag                                                                                                                                       <  1:781264/150‑3 (MQ=255)
              gCTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGttt                                                                                                                                    <  2:427660/150‑1 (MQ=255)
              gCTGGCACTGACCTGCCAGGCATTGGACCACGCCAGAATACAGCTGGACCAGCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGttt                                                                                                                                    <  2:566726/150‑1 (MQ=255)
                tGGCACTGGCCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGTGTGGATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGa                                                                                                                                  <  2:347063/150‑1 (MQ=255)
                  gCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACg                                                                                                                                >  2:1253441/1‑150 (MQ=255)
                  gCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACg                                                                                                                                >  2:1253461/1‑150 (MQ=255)
                    aCTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGAt                                                                                                                              >  2:870144/1‑150 (MQ=255)
                      tGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATa                                                                                                                             <  1:1152720/149‑1 (MQ=255)
                      tGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATa                                                                                                                             >  2:1152720/1‑149 (MQ=255)
                       gACCAGCCAGGCATTGGACAACGCCGGAATACAGGTGGACCATGCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGt                                                                                                                           <  2:652873/150‑1 (MQ=255)
                        aCCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTa                                                                                                                          >  1:216739/1‑150 (MQ=255)
                        aCCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTa                                                                                                                          >  1:675653/1‑150 (MQ=255)
                             ccAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGTGGCATTGACCCTGCGGTTTGACGATAGTATCGCt                                                                                                                     <  1:1062178/150‑1 (MQ=255)
                             ccAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGt                                                                                                                                      >  1:1387600/1‑133 (MQ=255)
                             ccAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGt                                                                                                                                      <  2:1387600/133‑1 (MQ=255)
                             ccAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGa                                                                                                                                  <  1:1043879/137‑1 (MQ=255)
                             ccAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGa                                                                                                                                  >  2:1043879/1‑137 (MQ=255)
                               aGGCATTGGACCACGCCGGAATATAAATGATACGGCGCCCTTGGCATGTACGCTACGACAGCGAACAGGCCTGTGCACGGGTGCATGGCCGATACTGGGGAAAACGCCTGCAGGAGCATTGACCCTGCGGTTTGACGACAGTATCGCATc                                                                                                                   <  2:617067/150‑1 (MQ=255)
                               aGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTc                                                                                                                   <  2:418343/150‑1 (MQ=255)
                                 gCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGc                                                                                                                 <  2:1142757/150‑1 (MQ=255)
                                        aCCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTAt                                                                                                          <  1:943061/150‑1 (MQ=255)
                                         ccACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATc                                                                                                         >  2:606590/1‑150 (MQ=255)
                                            cGCCGGAATACAGCGGGACCATCCCGCTTTGCATGTATGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGCACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAAt                                                                                                      <  2:261279/150‑1 (MQ=255)
                                              ccGGAATACAGCTGGACCATCCCGCTTGGCATGTCCGCTATGACAGCGAAGAGCCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCATGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTc                                                                                                    <  1:1359229/150‑1 (MQ=255)
                                                ggAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGt                                                                                                  >  2:864291/1‑150 (MQ=255)
                                                  attACAGATAGACCATCCCGCTTGACATGTACGTTATGACAGCGAAGAGGCCTGTGCACCGGTCTATGGATCGACCTGGGGTTAATGCCTGAAGAGCCAGTGCAAATGCGCTTTAACGATAGTACCGTTTCGCCCCCTATCAATTCCTGa                                                                                                <  2:221840/148‑1 (MQ=255)
                                                       aGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGc                                                                                           >  2:854110/1‑150 (MQ=255)
                                                        gCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGcc                                                                                          <  2:1232351/150‑1 (MQ=255)
                                                         cTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGGTTGACGATAGTATCGCGTCGCACCCTATCAATTCGTTACTGGCCg                                                                                         >  2:784617/1‑150 (MQ=255)
                                                             aCCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTcc                                                                                     <  1:864291/150‑1 (MQ=255)
                                                              ccATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTccc                                                                                    >  1:1401280/1‑150 (MQ=255)
                                                                aTCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGTCAATGCCCCTGca                                                                                                                                        <  1:105734/96‑2 (MQ=255)
                                                                aTCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGTCAATGCCCCTGca                                                                                                                                        >  2:105734/1‑95 (MQ=255)
                                                                aTCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGa                                                                                  <  1:1253461/150‑1 (MQ=255)
                                                                aTCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGa                                                                                  <  1:1253441/150‑1 (MQ=255)
                                                                 tCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCCGCAGGGTCAATGCCCCTGca                                                                                                                                        <  2:1065391/95‑2 (MQ=255)
                                                                 tCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCCGCAGGGTCAATGCCCCTGca                                                                                                                                        >  1:1065391/1‑94 (MQ=255)
                                                                  cccGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATc                                                                                >  2:889343/1‑150 (MQ=255)
                                                                  cccGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATc                                                                                >  1:126959/1‑150 (MQ=255)
                                                                     gCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGc                                                                             <  2:186356/150‑1 (MQ=255)
                                                                        tGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCaa                                                                          <  2:675653/150‑1 (MQ=255)
                                                                         ggCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAg                                                                         <  1:1078749/150‑1 (MQ=255)
                                                                            aTGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGAt                                                                      <  1:639052/150‑1 (MQ=255)
                                                                                    tATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCATGCACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAg                                                              <  1:784617/150‑1 (MQ=255)
                                                                                          aGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCa                                                        >  2:1347439/1‑150 (MQ=255)
                                                                                              aaGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGt                                                    <  1:854110/150‑1 (MQ=255)
                                                                                                   gCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCa                                               >  2:1337217/1‑150 (MQ=255)
                                                                                                     cTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCata                                             >  2:818590/1‑150 (MQ=255)
                                                                                                               gTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACagcagc                                   <  1:870144/150‑1 (MQ=255)
                                                                                                                   aTGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGTCCAGGCTGTATTATGTTGGCATGGTGCGCATATAACAGCAGCCCGa                               <  1:889343/150‑1 (MQ=255)
                                                                                                                    tGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGt                                                                                                  <  2:965562/82‑1 (MQ=255)
                                                                                                                    tGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGt                                                                                                  >  1:965562/1‑82 (MQ=255)
                                                                                                                     ggCGGGGACTGGAGAAAACGCCTGCAGGGTCATTGACCCGGCGGTTTGACGATAGTACCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGCCATGGTGCGCATATAACAGCAGGCCGAGc                             <  2:163218/150‑1 (MQ=255)
                                                                                                                           gACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCgg                       >  2:117208/1‑150 (MQ=255)
                                                                                                                            aCTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCggg                      >  2:498978/1‑150 (MQ=255)
                                                                                                                              tGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCat                                              >  2:547488/1‑124 (MQ=255)
                                                                                                                              tGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCat                                              <  1:547488/124‑1 (MQ=255)
                                                                                                                               ggggAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCAc                   <  1:818590/150‑1 (MQ=255)
                                                                                                                                   aaaaCGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGc               <  1:1347439/150‑1 (MQ=255)
                                                                                                                                     aaCGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCaa             >  1:443488/1‑150 (MQ=255)
                                                                                                                                     aaCGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCaa             <  2:216739/150‑1 (MQ=255)
                                                                                                                                      aCGCCTGCAGGGGCCTTGACCCTGCGGTTTGACGCTAGTATTGCTTCGCACCCTATCAATTAGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATAGGGCAAtttgaag            >  2:569232/1‑145 (MQ=255)
                                                                                                                                              aGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTc                                                                                                    <  2:510019/54‑1 (MQ=255)
                                                                                                                                              aGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTc                                                                                                    >  1:510019/1‑54 (MQ=255)
                                                                                                                                                gggCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCAAGACAATGAggg  >  2:327161/1‑150 (MQ=255)
                                                                                                                                                gggCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCAAGACAATGAggg  >  1:734531/1‑150 (MQ=255)
                                                                                                                                                gggCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCAAGACAATGAggg  >  2:73337/1‑150 (MQ=255)
                                                                                                                                                gggCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCAAGACAATGAggg  >  1:657066/1‑150 (MQ=255)
                                                                                                                                                  gCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAg                                                              >  2:845096/1‑88 (MQ=255)
                                                                                                                                                  gCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAg                                                              <  1:845096/88‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
GCAAAGGCACGTATGCTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCAAGACAATGAGGG  >  NC_002947/3982733‑3983026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑