Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,982,885	A→C	8.4%	*120C (TGA→TGC)	PP_3510 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,982,885	0	A	C	8.4%	182.7 / 7.4	70	*120C (TGA→TGC)	PP_3510	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (30/34);  new base C (2/4);  total (32/38)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

TATGCTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCAAGACAATGAGGGTGTCATGA  >  NC_002947/3982744‑3983034                                                                                                                                              |                                                                                                                                                      tATGCTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGTCAATGCCCCTGcag                                                                                                                                               <  1:781264/150‑3 (MQ=255)    gCTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGttt                                                                                                                                            <  2:427660/150‑1 (MQ=255)    gCTGGCACTGACCTGCCAGGCATTGGACCACGCCAGAATACAGCTGGACCAGCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGttt                                                                                                                                            <  2:566726/150‑1 (MQ=255)      tGGCACTGGCCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGTGTGGATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGa                                                                                                                                          <  2:347063/150‑1 (MQ=255)        gCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACg                                                                                                                                        >  2:1253441/1‑150 (MQ=255)        gCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACg                                                                                                                                        >  2:1253461/1‑150 (MQ=255)          aCTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGAt                                                                                                                                      >  2:870144/1‑150 (MQ=255)            tGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATa                                                                                                                                     >  2:1152720/1‑149 (MQ=255)            tGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATa                                                                                                                                     <  1:1152720/149‑1 (MQ=255)             gACCAGCCAGGCATTGGACAACGCCGGAATACAGGTGGACCATGCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGt                                                                                                                                   <  2:652873/150‑1 (MQ=255)              aCCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTa                                                                                                                                  >  1:675653/1‑150 (MQ=255)              aCCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTa                                                                                                                                  >  1:216739/1‑150 (MQ=255)                   ccAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGTGGCATTGACCCTGCGGTTTGACGATAGTATCGCt                                                                                                                             <  1:1062178/150‑1 (MQ=255)                   ccAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGt                                                                                                                                              >  1:1387600/1‑133 (MQ=255)                   ccAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGt                                                                                                                                              <  2:1387600/133‑1 (MQ=255)                   ccAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGa                                                                                                                                          >  2:1043879/1‑137 (MQ=255)                   ccAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGa                                                                                                                                          <  1:1043879/137‑1 (MQ=255)                     aGGCATTGGACCACGCCGGAATATAAATGATACGGCGCCCTTGGCATGTACGCTACGACAGCGAACAGGCCTGTGCACGGGTGCATGGCCGATACTGGGGAAAACGCCTGCAGGAGCATTGACCCTGCGGTTTGACGACAGTATCGCATc                                                                                                                           <  2:617067/150‑1 (MQ=255)                     aGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTc                                                                                                                           <  2:418343/150‑1 (MQ=255)                       gCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGc                                                                                                                         <  2:1142757/150‑1 (MQ=255)                              aCCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTAt                                                                                                                  <  1:943061/150‑1 (MQ=255)                               ccACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATc                                                                                                                 >  2:606590/1‑150 (MQ=255)                                  cGCCGGAATACAGCGGGACCATCCCGCTTTGCATGTATGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGCACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAAt                                                                                                              <  2:261279/150‑1 (MQ=255)                                    ccGGAATACAGCTGGACCATCCCGCTTGGCATGTCCGCTATGACAGCGAAGAGCCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCATGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTc                                                                                                            <  1:1359229/150‑1 (MQ=255)                                      ggAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGt                                                                                                          >  2:864291/1‑150 (MQ=255)                                        attACAGATAGACCATCCCGCTTGACATGTACGTTATGACAGCGAAGAGGCCTGTGCACCGGTCTATGGATCGACCTGGGGTTAATGCCTGAAGAGCCAGTGCAAATGCGCTTTAACGATAGTACCGTTTCGCCCCCTATCAATTCCTGa                                                                                                        <  2:221840/148‑1 (MQ=255)                                             aGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGc                                                                                                   >  2:854110/1‑150 (MQ=255)                                              gCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGcc                                                                                                  <  2:1232351/150‑1 (MQ=255)                                               cTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGGTTGACGATAGTATCGCGTCGCACCCTATCAATTCGTTACTGGCCg                                                                                                 >  2:784617/1‑150 (MQ=255)                                                   aCCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTcc                                                                                             <  1:864291/150‑1 (MQ=255)                                                    ccATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTccc                                                                                            >  1:1401280/1‑150 (MQ=255)                                                      aTCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGTCAATGCCCCTGca                                                                                                                                                <  1:105734/96‑2 (MQ=255)                                                      aTCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGTCAATGCCCCTGca                                                                                                                                                >  2:105734/1‑95 (MQ=255)                                                      aTCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGa                                                                                          <  1:1253441/150‑1 (MQ=255)                                                      aTCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGa                                                                                          <  1:1253461/150‑1 (MQ=255)                                                       tCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCCGCAGGGTCAATGCCCCTGca                                                                                                                                                <  2:1065391/95‑2 (MQ=255)                                                       tCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCCGCAGGGTCAATGCCCCTGca                                                                                                                                                >  1:1065391/1‑94 (MQ=255)                                                        cccGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATc                                                                                        >  2:889343/1‑150 (MQ=255)                                                        cccGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATc                                                                                        >  1:126959/1‑150 (MQ=255)                                                           gCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGc                                                                                     <  2:186356/150‑1 (MQ=255)                                                              tGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCaa                                                                                  <  2:675653/150‑1 (MQ=255)                                                               ggCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAg                                                                                 <  1:1078749/150‑1 (MQ=255)                                                                  aTGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGAt                                                                              <  1:639052/150‑1 (MQ=255)                                                                          tATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCATGCACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAg                                                                      <  1:784617/150‑1 (MQ=255)                                                                                aGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCa                                                                >  2:1347439/1‑150 (MQ=255)                                                                                    aaGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGt                                                            <  1:854110/150‑1 (MQ=255)                                                                                         gCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCa                                                       >  2:1337217/1‑150 (MQ=255)                                                                                           cTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCata                                                     >  2:818590/1‑150 (MQ=255)                                                                                                     gTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACagcagc                                           <  1:870144/150‑1 (MQ=255)                                                                                                         aTGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGTCCAGGCTGTATTATGTTGGCATGGTGCGCATATAACAGCAGCCCGa                                       <  1:889343/150‑1 (MQ=255)                                                                                                          tGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGt                                                                                                          <  2:965562/82‑1 (MQ=255)                                                                                                          tGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGt                                                                                                          >  1:965562/1‑82 (MQ=255)                                                                                                           ggCGGGGACTGGAGAAAACGCCTGCAGGGTCATTGACCCGGCGGTTTGACGATAGTACCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGCCATGGTGCGCATATAACAGCAGGCCGAGc                                     <  2:163218/150‑1 (MQ=255)                                                                                                                 gACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCgg                               >  2:117208/1‑150 (MQ=255)                                                                                                                  aCTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCggg                              >  2:498978/1‑150 (MQ=255)                                                                                                                    tGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCat                                                      >  2:547488/1‑124 (MQ=255)                                                                                                                    tGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCat                                                      <  1:547488/124‑1 (MQ=255)                                                                                                                     ggggAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCAc                           <  1:818590/150‑1 (MQ=255)                                                                                                                         aaaaCGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGc                       <  1:1347439/150‑1 (MQ=255)                                                                                                                           aaCGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCaa                     <  2:216739/150‑1 (MQ=255)                                                                                                                           aaCGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCaa                     >  1:443488/1‑150 (MQ=255)                                                                                                                            aCGCCTGCAGGGGCCTTGACCCTGCGGTTTGACGCTAGTATTGCTTCGCACCCTATCAATTAGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATAGGGCAAtttgaag                    >  2:569232/1‑145 (MQ=255)                                                                                                                                    aGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTc                                                                                                            >  1:510019/1‑54 (MQ=255)                                                                                                                                    aGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTc                                                                                                            <  2:510019/54‑1 (MQ=255)                                                                                                                                      gggCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCAAGACAATGAggg          >  2:73337/1‑150 (MQ=255)                                                                                                                                      gggCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCAAGACAATGAggg          >  1:734531/1‑150 (MQ=255)                                                                                                                                      gggCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCAAGACAATGAggg          >  1:657066/1‑150 (MQ=255)                                                                                                                                      gggCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCAAGACAATGAggg          >  2:327161/1‑150 (MQ=255)                                                                                                                                        gCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAg                                                                      <  1:845096/88‑1 (MQ=255)                                                                                                                                        gCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAg                                                                      >  2:845096/1‑88 (MQ=255)                                                                                                                                              aCCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCAAGACAATGAGGGTGTCATGa  <  2:734531/150‑1 (MQ=255)                                                                                                                                              aCCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGACCGAGCGATCGGGCACTTGCAATGTAAGGAGGGTGTCATGa  <  2:126959/150‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                      TATGCTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCAAGACAATGAGGGTGTCATGA  >  NC_002947/3982744‑3983034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑