Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,038,611 C→G 9.8% A122A (GCC→GCG) ‡ PP_3559 → glycine betaine ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,038,6110CG 9.8% 147.9 / 8.2 51A122A (GCC→GCG) ‡PP_3559glycine betaine ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (24/22);  new base G (2/3);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.97e-01

GCTGCGCAGTGCCGTGCTGGTCGCGCTGCTGGCCCTGATCGGTATGCTCGGGCTCTGGGACAAGTTGCTGCAGACCCTGGCCCTGGTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGCCGGTCCTCGATGTGATGCAGACGCTGCCTGCGTTCGTTTACCTGATCCCGGTGCTGATGCTGTTTGGCCTGGGCAAGGTGCCTGCCGTGTTCGCCACGCTGAT  >  NC_002947/4038464‑4038742
                                                                                                                                                   |                                                                                                                                   
gctgcGCAGTGCCGTGCTGGTCGCGCTGCTGGCCCTGATCGGTATGCTCGGGCTCTGGGACAAGTTGCTGCAGACCCTGGCCCTGGTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCg                                                                                                                                   <  2:1292268/150‑1 (MQ=255)
       aGTGCCGTGCTGGTCGCGCTGCTGGCCCTGATCGGTATGCTCGGGCTCTGGGACAAGTTGCTGCAGACCCTGGCCCTGGTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGGGCGGCCagca                                                                                                                            <  1:110334/150‑4 (MQ=255)
       aGTGCCGTGCTGGTCGCGCTGCTGGCCCTGATCGGTATGCTCGGGCTCTGGGACAAGTTGCTGCAGACCCTGGCCCTGGTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTg                                                                                                                            <  1:233218/150‑1 (MQ=255)
            cGTGCTGGTCGCGCTGCTGGCCCTGATCGGTATGCTCGGGCTCTGGGACAAGTTGCTGCAGACCCTGGCCCTGGTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAg                                                                                                                       >  2:274603/1‑150 (MQ=255)
             gTGCTGGTCGCGCTGCTGGCCCTGATCGGTATGCTCGGGCTCTGGGACAAGTTGCTGCAGACCCTGGCCCTGGTGCTGGTCAGTAACGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCTTCCTTCTGGCCGCCCGGCCACTGGCCAGa                                                                                                                      >  1:931423/1‑150 (MQ=255)
                    tCGCGCTGCTGGACCTGATCCGTATGCTCGGGCTCTGGGACCAGTTGCTGCAGACCCTGGCCCTGGTGCTGGTCAGTACCGGTTTGTCTGGACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGgctgc                                                                                                               <  1:429229/150‑1 (MQ=255)
                     cgcgCTGCTGGCCCTGATCGGTATGCTCGGGCTCTGGGACAAGTTGCTGCAGACCCTGGCCCTGGTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGgctgct                                                                                                              <  2:1243730/150‑1 (MQ=255)
                       cgcTGCTGGCCCTGATCGGTATGCTCGGGCTCTGGGACAAGTTGCTGCAGACCCTGGCCCTGGTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGt                                                                                                            >  1:103423/1‑150 (MQ=255)
                          tgctgGCCCTGATCGGTATGCTCGGGCTCTGGGACAAGTTGCTGCAGACCCTGGCCCTGGTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGc                                                                                                         <  1:912561/150‑1 (MQ=255)
                          tgctgGCCCTGATCGGTATGCTCGGGCTCTGGGACAAGTTGCTGCAGACCCTGGCCCTGGTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGc                                                                                                         <  1:274603/150‑1 (MQ=255)
                             tgGCCCTGATCGGTATGCTCGGGCTCTGGGACAAGTTGCTGCAGACCCTGGCCCTGGTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGCCgg                                                                                                      <  2:615108/150‑1 (MQ=255)
                                  cTGATCGGTATGCTCGGGCTCTGGGACAAGTTGCTGCAGACCCTGGCCCTGGTGCTGGTAAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGGGCGGCCagca                                                                                                                            <  1:744272/123‑4 (MQ=255)
                                  cTGATCGGTATGCTCGGGCTCTGGGACAAGTTGCTGCAGACCCTGGCCCTGGTGCTGGTAAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGGGCGGCCagca                                                                                                                            >  2:744272/1‑120 (MQ=255)
                                       cGGTATGCTCGGGCTCTGGGACAAGTTGCTGCAGACCCTAGACTTGGTCCTCCAGGGCTTTAGTTTGGTCAGAGTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGCCGGTCCTCGAtgt                                                                                            <  1:168182/150‑1 (MQ=255)
                                            tGCTCGGGCTCTGGGACAAGTTGCTGCAGACCCTGGCCCTGGTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGCCGGTCCTCGATGTGATGc                                                                                       >  1:563130/1‑150 (MQ=255)
                                              cTCGGGCTCTGGGACAAGTTGCTGCAGACCCTGGCCCTGGTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGCCGGTCCTCGAtg                                                                                             <  2:51185/142‑1 (MQ=255)
                                              cTCGGGCTCTGGGACAAGTTGCTGCAGACCCTGGCCCTGGTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGCCGGTCCTCGAtg                                                                                             >  1:51185/1‑142 (MQ=255)
                                                 gggCTCTGGGACAAGTTGCTGCAGACCCTGGCCCTGGTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGc                                                                                                                          >  1:1351364/1‑110 (MQ=255)
                                                 gggCTCTGGGACAAGTTGCTGCAGACCCTGGCCCTGGTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGc                                                                                                                          <  2:1351364/110‑1 (MQ=255)
                                                       tGGGACAAGTTGCTGCAGACCCTGGCCCTGGTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCAGTGGGCATCCTGCTGGCCGCCCGACCACTGGCCAGACGGCTGCTGTTGACGGTCCTAGATCTGATGCAGAAGgtgcgt                                                                            >  2:277410/1‑148 (MQ=255)
                                                       tGGGACAAGTTGCTGCAGACCCTGGCCCTGGTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGCCGGTCCTCGATGTGATGCAGACGctgcct                                                                            <  1:277410/150‑1 (MQ=255)
                                                             aaGTTGCTGCAGACCCTGGCCCTGGTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGCCGGTCCTCGATGTGATGCAGACGCTGCCTGcgttc                                                                      >  2:1313471/1‑150 (MQ=255)
                                                                ttGCTGCAGACCCTGGCCCTGGTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGCCGGTCCTCGATGTGATGCAGACGCTGCCTGcgttcgtt                                                                   >  1:769765/1‑150 (MQ=255)
                                                                   ctgcAGACCCTGGCCCTGGTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGCCGGTCCTCGATGTGATGCAGACGCTGCCTGCGTTCGTTTAc                                                                >  1:465053/1‑150 (MQ=255)
                                                                       aGACCCTGGCCCTGGTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGGGCGGCCag                                                                                                                              <  1:1165020/84‑2 (MQ=255)
                                                                       aGACCCTGGCCCTGGTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGGGCGGCCag                                                                                                                              >  2:1165020/1‑83 (MQ=255)
                                                                       aGACCCTGGCCCTGGTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGCCGGTCCTCGATGTGATGCAGACGCTGCCTGCGTTCGTTTACCTGa                                                            <  1:1148541/150‑1 (MQ=255)
                                                                           cctggccctggTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGtt                                                                                                           <  2:392290/99‑1 (MQ=255)
                                                                           cctggccctggTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGtt                                                                                                           >  1:392290/1‑99 (MQ=255)
                                                                           cctggccctggTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGCCGGTCCTCGATGTGATGCAGACGCTGCCTGcgttc                                                                      <  1:1176154/136‑1 (MQ=255)
                                                                           cctggccctggTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGCCGGTCCTCGATGTGATGCAGACGCTGCCTGcgttc                                                                      >  2:1176154/1‑136 (MQ=255)
                                                                           cctggccctggTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGCCGGTCCTCGATGTGATGCAGACGCTGCCTGCGTTCGTTTACCTGATccc                                                        >  2:988429/1‑150 (MQ=255)
                                                                            ctggccctggTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGCCGGTCCTCGATGTGATGCAGACGCTGCCTGCGTTCGTTTACCTGATCCCg                                                       <  2:103423/150‑1 (MQ=255)
                                                                               gccctggTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGCCGGTCCTCGATGTGATGCAGACGCTGCCTGCGTTCGTTTACCTGATCCCGGTg                                                    >  2:258271/1‑150 (MQ=255)
                                                                                ccctggTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGCCGGTCCTCGATGTGATGCAGACGCTGCCTGCGTTCGTTTACCTGATCCCGGTGc                                                   <  1:1313471/150‑1 (MQ=255)
                                                                                ccctggTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGCCGGTCCTCGATGTGATGCAGACGCTGCCTGCGTTCGTTTACCTGATCCCGGTGc                                                   >  2:189034/1‑150 (MQ=255)
                                                                                ccctggTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGCCGGTCCTCGATGTGATGCAGACGCTGCCTGCGTTCGTTTACCTGATCCCGGTGc                                                   <  1:988429/150‑1 (MQ=255)
                                                                                 cctggTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGCCGGTCCTCGATGTGATGCAGACGCTGCCTGCGTTCGTTTACCTGATCCCGGTGCt                                                  >  1:542652/1‑150 (MQ=255)
                                                                                 cctggTGCTGGTCAGTACCGGTTTGTGCGCACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGCCGCTCCTCGATGTGATGCAGACGCTGCCTGCGTTCGTTTACCTGATCCCGGTGCt                                                  >  2:69873/1‑150 (MQ=255)
                                                                                   tggTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGCCGGTCCTCGATGTGATGCAGACGCTGCCTGCGTTCGTTTACCTGATCCCGGTGCTGa                                                >  1:797793/1‑150 (MQ=255)
                                                                                          ggTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGCCGGTCCTCGATGTGATGCAGACGCTGCCTGCGTTCGTTTACCTGATCCCGGTGCTGATGCTGtt                                         <  2:1215659/150‑1 (MQ=255)
                                                                                                     gTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGctgctg                                                                                                             <  2:392883/71‑1 (MQ=255)
                                                                                                     gTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGACAGACGGctgctg                                                                                                             >  1:392883/1‑71 (MQ=255)
                                                                                                                   gtgggtgTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGCCGGTCCTCGATGTGATGCAGACGCTGCCTGcgttcgt                                                                    <  1:600226/98‑1 (MQ=255)
                                                                                                                   gtgggtgTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGCCGGTCCTCGATGTGATGCAGACGCTGCCTGcgttcgt                                                                    >  2:600226/1‑98 (MQ=255)
                                                                                                                   gtgggtgTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGCCGGTCCTCGATGTGATGCAGACGCTGCCTGCGTTCGTTTACCTGATCCCGGTGCTGATGCTGTTTGGCCTGGGCAAGGTGCCTGCCgtg                >  1:710992/1‑150 (MQ=255)
                                                                                                                             cACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGCCGGTCCTCGATGTGATGCAGACGCTGCCTGCGTTCGTTTACCTGATCCCGGTGCTGATGCTGTTTGGCCTGGGCAAGGTGCCTGCCGTGTTCGCCACGc      >  1:64813/1‑150 (MQ=255)
                                                                                                                             cACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGCCGGTCCTCGATGTGATGCAGACGCTGCCTGCGTTCGTTTACCTGATCCCGGTGCTGATGCTGTTTGGCCTGGGCAAGGTGCCTGCCGTGTTCGCCACGc      <  1:258271/150‑1 (MQ=255)
                                                                                                                                tGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGCCGGTCCTCGATGTGATGCAGACGCTGCCTGCGTTCGTTTACCTGATCCCGGTGCTGATg                                              <  2:167108/107‑1 (MQ=255)
                                                                                                                                tGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGCCGGTCCTCGATGTGATGCAGACGCTGCCTGCGTTCGTTTACCTGATCCCGGTGCTGATg                                              >  1:167108/1‑107 (MQ=255)
                                                                                                                                 gggCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGCCGGTCCTCGATGTGATGCAGACGCTGCCTGCGTTCGTTTACCTGATCCCGGTGCTGATGCTGTTTGGCCTGGGCAAGGTGCCTGCCGTGTTCGCCACGCTGAt  >  2:876146/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                   
GCTGCGCAGTGCCGTGCTGGTCGCGCTGCTGGCCCTGATCGGTATGCTCGGGCTCTGGGACAAGTTGCTGCAGACCCTGGCCCTGGTGCTGGTCAGTACCGGTTTGTGCGTACTGGTGGGTGTACCACTGGGCATCCTGCTGGCCGCCCGGCCACTGGCCAGACGGCTGCTGTTGCCGGTCCTCGATGTGATGCAGACGCTGCCTGCGTTCGTTTACCTGATCCCGGTGCTGATGCTGTTTGGCCTGGGCAAGGTGCCTGCCGTGTTCGCCACGCTGAT  >  NC_002947/4038464‑4038742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑