| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,049,646 | A→T | 6.7% | K718M (AAG→ATG) | quiA → | quinate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,049,646 | 0 | A | T | 6.7% | 175.9 / 2.2 | 56 | K718M (AAG→ATG) | quiA | quinate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (30/22); new base T (2/2); total (32/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGG > NC_002947/4049499‑4049792 | tGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGa > 1:751989/1‑150 (MQ=255) gtcgCCGGCATTCCAGGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGc < 2:1349017/150‑1 (MQ=255) cGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGcc < 2:1041933/150‑1 (MQ=255) cGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGcc < 1:1415184/150‑1 (MQ=255) ccATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCtt < 1:1316719/150‑1 (MQ=255) ccATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCtt < 1:1337658/150‑1 (MQ=255) gCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGTAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCAcc > 1:436808/1‑150 (MQ=255) ttttGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTCTCGAcc > 1:1119724/1‑150 (MQ=255) cACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAgg > 1:216027/1‑150 (MQ=255) cccTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAggcg < 2:1119724/150‑1 (MQ=255) ccATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGgtgt > 2:966340/1‑150 (MQ=255) aTGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCTGCATCCGCATGCAAGTGCCGCTCTTGCTCGGACAGATAGCGGGTGTCGTCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCa < 1:26860/150‑1 (MQ=255) aGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTa > 2:827201/1‑150 (MQ=255) gACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTAc > 1:1052864/1‑150 (MQ=255) gACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTAc > 2:1043504/1‑150 (MQ=255) cccGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCaa > 1:879288/1‑82 (MQ=255) cccGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCaa < 2:879288/82‑1 (MQ=255) ccGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCTTGATCGGcag > 1:705533/1‑88 (MQ=255) ccGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCTTGATCGGcag < 2:705533/90‑3 (MQ=255) ccGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCGGCTACTGGTCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCa < 2:1416877/150‑1 (MQ=255) ccGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCa > 1:452912/1‑150 (MQ=255) gTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAgg < 1:262692/116‑1 (MQ=255) gTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAgg > 2:262692/1‑116 (MQ=255) tGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTg > 2:636750/1‑150 (MQ=255) ggTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGa < 2:436808/150‑1 (MQ=255) cACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGAcagcag > 2:690239/1‑150 (MQ=255) aCCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACagcagc > 2:942361/1‑150 (MQ=255) atcgAAGACACCGGCCCGATCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAAc < 2:295397/147‑1 (MQ=255) aaGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCTCAGGTACCCAGGACTTCTACCTGCGGTCCTACGACAGCAGCAACGGTa > 2:654528/1‑150 (MQ=255) aaGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGt < 2:875598/149‑1 (MQ=255) aaGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGt > 1:875598/1‑149 (MQ=255) aGACACCGGCCCGCTCGACATCCGCATGCACCTCCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCCACCCAAGGCGGCCTGGTGTTCATCGCACGTACCCAGGACTTCTACCCGCGCGCCTACGACAGCCGCAACgagag > 1:295397/1‑146 (MQ=255) gACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGCGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAAc > 1:699683/1‑150 (MQ=255) acCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGaa > 1:1239215/1‑150 (MQ=255) acCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGaa > 1:586489/1‑150 (MQ=255) acCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGaa > 2:393235/1‑150 (MQ=255) ccGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGaaa < 1:690239/150‑1 (MQ=255) ggCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGAcagc < 2:1381363/132‑1 (MQ=255) ggCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGAcagc > 1:1381363/1‑132 (MQ=255) ccGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTgg > 2:640964/1‑150 (MQ=255) gCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGaa < 1:827201/150‑1 (MQ=255) cGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAAGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGGCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGc > 2:580325/1‑150 (MQ=255) ggCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTAGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTAc < 1:720920/105‑1 (MQ=255) ggCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTAGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTAc > 2:720920/1‑105 (MQ=255) aTCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGAcagc < 2:636990/120‑1 (MQ=255) aTCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGAcagc > 1:636990/1‑120 (MQ=255) aTCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGc > 1:1284102/1‑150 (MQ=255) aTCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGc > 2:1315975/1‑150 (MQ=255) tCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGcc > 2:1122257/1‑150 (MQ=255) gCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGc < 2:216027/150‑1 (MQ=255) aTGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGccc < 2:905021/150‑1 (MQ=255) aCCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGa > 1:350204/1‑132 (MQ=255) aCCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGa < 2:350204/132‑1 (MQ=255) caggCCGATCTTGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTggg > 1:742620/4‑150 (MQ=255) tGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCa < 2:1291279/150‑1 (MQ=255) gATCAAGATCGGCCTGCCAACCCTTGGCGTCACCCTCTCGACCCAAGGCGGCCTGGTGTTCATAACAGGTACCCGGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCa > 2:695766/1‑150 (MQ=255) gATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGCGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCa < 2:699683/150‑1 (MQ=255) tCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTCACGCGAGCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAgg < 1:337587/150‑1 (MQ=255) | TGTCGGTCGCCGGCATTCCATGCCAGGCACCGCCGTTTGGCACCCTGACCGCCATCGACATGAAGACCCGCCAGGTGGCCTGGCAGGTGCCGGTAGGCACCGTTGAAGACACCGGCCCGCTCGGCATCCGCATGCACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGG > NC_002947/4049499‑4049792 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |