Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,073,167 C→A 7.4% V114F (GTC→TTC) ‡ mdtA ← multidrug transporter membrane fusion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,073,1670CA 7.4% 167.6 / 3.4 55V114F (GTC→TTC) ‡mdtAmultidrug transporter membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base C (28/22);  new base A (2/2);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.99e-01

TCTTCAGCGTACAGGCCTTTATAGCGGGCCAGGTCGACCTGAGCGTTCTTCAACTGTGCCTGGTTCTGCGCCAAGGTGCCTTCGGCCTGCTGCAGGGCGATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCGTATTGGTCGCGGTAACCGTGCCCAAGGCTTTGTAGTAGAGGGGGAAGTCGCCTATCCGCACCGGCACGACGCGTACGGGTAC  >  NC_002947/4073019‑4073314
                                                                                                                                                     |                                                                                                                                                   
tCTTCAGCGTACAGGCCTTTATAGCGGGCCAGTTCGACCTGAGCGTTCTTCAACTGTGCCTGGTTCTGCGCCAAGGTGCCTTCGGCCTGCTGCAGGGCGATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGAcg                                                                                                                                                    >  1:608712/1‑150 (MQ=255)
  ttCAGCGTACAGGCCTTTATAGCGGGCCAGGTCGACCTGAGCGTTCTTCAACTGTGCCTGGTTCTGCGCTAAGGTGCCAACACGGAAGAGCAGGGGTATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCCGGTCGCCAGC‑CTTGAcgcg                                                                                                                                                  >  1:461571/1‑150 (MQ=255)
      gCGTACAGGCCTTTATAGCGGGCCAGGTCGACCTGAGCGTTCTTCAACTGTGCCTGGTTCTGCGCCAAGGTGCCTTCGGCCTGCTGCAGGGCGATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTgg                                                                                                                                              >  1:553326/1‑150 (MQ=255)
         tACAGGCCTTTATAGCGGGCCAGGTCGACCTGAGCGTTCTTCAACTGTGCCTGGTTCTGCGCCAAGGTGCCTTCGGCCTGCTGCAGGGCGATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGcct                                                                                                                                           >  1:285547/1‑150 (MQ=255)
            aGGCCTTTATAGCGGGCCAGGTCGACCTGAGCGTTCTTCAACTGTGCCTGGTTCTGCGCCAAGGTGCCTTCGGCCTGCTGCAGGGCGATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGccttcc                                                                                                                                        >  1:1016686/1‑150 (MQ=255)
                 tttATAGCGGGCCAGGTCGACCTGAGCGTTCTTCAACTGTGCCTGGTTCTGCGCCCAGGTGCCTTCGGCCTGCTGCAGGGCGATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGaa                                                                                                                                   >  1:1243522/1‑150 (MQ=255)
                 tttATAGCGGGCCAGGTCGACCTGAGCGTTCTTCAACTGTGCCTGGTTCTGCGCCAAGGTGCCTTCGGCCTGCTGCAGGGCGATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGaa                                                                                                                                   >  2:121208/1‑150 (MQ=255)
                 tttATAGCGGGCCAGGTCGACCTGAGCGTTCTTCAACTGTGCCTGGTTCTGCGCCAAGGTGCCTTCGGCCTGCTGCAGGGCGATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGaa                                                                                                                                   <  1:149792/150‑1 (MQ=255)
                 tctataGCGGGCCAGGTCGACCTGAGCGTTCTTCAACTGTGCCTGGTTCTGCGCCAAGGTGCCTTCGGCCTGCTGCAGGGCGATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGaa                                                                                                                                   >  1:141104/3‑150 (MQ=255)
                    ataGCGGGCCAGGTCGACCTGAGCGTTCTTCAACTGTGCCTGGTTCTGCGCCAAGGTGCCTTCGGCCTGCTGCAGGGCGATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGATGCGCTGGCCTTCCTTGAAGTg                                                                                                                                <  1:1268267/150‑1 (MQ=255)
                                      cTGAGCGTTCTTCAACTGTGCCTGGTTCTGCGCCAAGGTGCCTTCGGCCTGCTGCAGGGCGATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCgcc                                                                                                              >  1:108441/1‑150 (MQ=255)
                                              tcttcAACTGTGCCTGGTTCTGCGCCAAGGTGCCTTCGGCCTGCTGCAGGGCGATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCAcgc                                                                                                      <  2:608712/150‑1 (MQ=255)
                                              tcttaAACTGTGCCTGGTTCTGCGCCAAGGCGCCTTCGGCCTGCGGCAGGTCGATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCAcgc                                                                                                      <  2:1016686/150‑1 (MQ=255)
                                                  cAACTGTGCCTGGTTCTGCGCCAAGGTGCCTTCGGCCTGCTGCAGGGCGATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTCCGCCAGCCACGCGGCt                                                                                                  <  2:461571/150‑1 (MQ=255)
                                                           cTGGTTCTGCGCCAAGGTGCCTTCGGCCTGCTGCAGGGCGATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACAtt                                                                                         >  2:278422/1‑150 (MQ=255)
                                                            tGGTTCTGCGCCAAGGTGCCTTCGGCCTGCTGCAGGGCGATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGGTTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTg                                                                                        >  1:12778/1‑150 (MQ=255)
                                                               ttCTGCGCCAAGGTGCCTTCGGCCTGCTGCAGGGCGATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGAcc                                                                                     <  2:841669/150‑1 (MQ=255)
                                                               ttCTGCGCCAAGGTGCCTTCGGCCTGCTGCAGGGCGATGCGATAAGGGCGCGGGGCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGAcc                                                                                     >  2:1155124/1‑150 (MQ=255)
                                                                tCTGCGCCAAGGTGCCTTCGGCCTGCTGCAGGGCGATGCGATAAGGGAGCGGGTCGATTTCGGCCAGCAGGTCGACAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCg                                                                                    <  1:1387081/150‑1 (MQ=255)
                                                                  tGCGCCAAGGTGCCTTCGGCCTGCTGCAGGGCGATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCGTa                                                                                  >  1:269748/1‑150 (MQ=255)
                                                                     gcCAAGGTGCCTTCGGCCTGCTGCAGGGCGATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAg                                                                                                                                  <  1:926805/99‑1 (MQ=255)
                                                                     gcCAAGGTGCCTTCGGCCTGCTGCAGGGCGATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAg                                                                                                                                  >  2:926805/1‑99 (MQ=255)
                                                                            tGCCTTCGGCCTGCTGCAGGGCGATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCGTATTGGTCGCgg                                                                        <  2:1243522/150‑1 (MQ=255)
                                                                              ccTTCGGCCTGCTGCAGGGCGATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTg                                                                                                                                     >  1:60750/1‑87 (MQ=255)
                                                                              ccTTCGGCCTGCTGCAGGGCGATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTg                                                                                                                                     <  2:60750/87‑1 (MQ=255)
                                                                              ccTTCGGCCTGCTGCAGGGCGATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCGTATTGGTCGCGGTa                                                                      >  2:1219476/1‑150 (MQ=255)
                                                                                ttCGGCCTGCTGCAGGGCGATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCGTATTGGTCGCGGTAAc                                                                    >  1:82502/1‑150 (MQ=255)
                                                                                 tCGGCCTGCTGCAGGGCGATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCGTATTGGTCGCGGTAAcc                                                                   <  1:121208/150‑1 (MQ=255)
                                                                                   ggCCTGCTGCAGGGCGATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCGTATTGGTCGCGGTAACCGt                                                                 <  2:315875/150‑1 (MQ=255)
                                                                                   ggCCTGCTGCAGGGCGATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCGTATTGGTCGCGGTAACCGt                                                                 >  1:315875/1‑150 (MQ=255)
                                                                                    gCCTGCTGCAGGGCGATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTg                                                                                        >  2:651584/1‑126 (MQ=255)
                                                                                    gCCTGCTGCAGGGCGATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTg                                                                                        <  1:651584/126‑1 (MQ=255)
                                                                                     ccTGCTGCAGGGCGATGCGATAAGGGCGCGGCTAGATTTCGGAGAGCAGCTCGCCCGC‑GTTGACGCTCTGGCCGTCCGGGAACGGGATGTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCGTATTGGTCGCGGTAACCGTGc                                                               >  2:1230933/1‑150 (MQ=255)
                                                                                               ggCGATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCGTATTGGTCGCGGTAACCGTGCCCAAGGCttt                                                     <  2:553326/150‑1 (MQ=255)
                                                                                                   atgcgatAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCGTATTGGTCGCGGTAACCGTGCCCAAGGCTTTgtag                                                 >  1:990622/1‑150 (MQ=255)
                                                                                                    tgcgatAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCGTATTGGTCGCGGTAACCGTGCCCAAGGCTTTgtagt                                                <  1:1219476/150‑1 (MQ=255)
                                                                                                        atAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCGTATTGGTCGCGGTAACCGTGCCCAAGGCTTTGTAGTagag                                            >  1:1266039/1‑150 (MQ=255)
                                                                                                          aaGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCGTATTGGTCGCGGTAACCGTGCCCAAGGCTTTGTAGTAGAggg                                          <  1:1301316/150‑1 (MQ=255)
                                                                                                             gaactggtgaaaatccacttcaaggaagGCCAGCGCGTCAAG‑GCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCGTATTGGTCGCGGTAACCGTGCCCAAGGCTTTGTAGTAGAgg                                           <  1:172481‑M1/118‑1 (MQ=255)
                                                                                                             gaactggtgaaaatccacttcaaggaagGCCAGCGCGTCAAG‑GCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCGTATTGGTCGCGGTAACCGTGCCCAAGGCTTTGTAGTAGAgg                                           >  2:172481‑M1/29‑146 (MQ=255)
                                                                                                                      cGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCGTATTGGTCGCGGTAACTGTGCCCAAGGCTTTGTAGTAGAGGGGGAAGTCGCCTa                              <  1:1155124/150‑1 (MQ=255)
                                                                                                                         tttCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCGTATTGGTCGCGGTAACCGTGCCCAAGGCTTTGTAGTAGAGGGGGAAGTCGCCTATcc                           >  2:630093/1‑150 (MQ=255)
                                                                                                                             ggCCAGCAGGTCGCCAGC‑CTTGACGCGNTGGCCTTCCTTGNAGTGGANTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCGTATTGGTCGCGGTAACCGTGCCCACGGCTTTGTAGTAGAGGGGGAAGTCGCCTATCCGCAc                       >  1:1139595/1‑150 (MQ=255)
                                                                                                                               ttcaaggaagGCCAGCGCGTCAAG‑GCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCGTATTGGTCGCGGTAACCGTGCCCAAg                                                          >  2:355632‑M1/11‑113 (MQ=255)
                                                                                                                               ttcaaggaagGCCAGCGCGTCAAG‑GCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCGTATTGGTCGCGGTAACCGTGCCCAAg                                                          <  1:355632‑M1/103‑1 (MQ=255)
                                                                                                                                 agcagGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCGTATTGGTCGCGGTAACCGTGCCCAAGGCTTTGTAGTAGAGGGGGAAGTCGCCTATCCGCACCGGc                   >  1:765371/1‑150 (MQ=255)
                                                                                                                                      gTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCATTTTGACCGTATTGGTCGCGGTAACCGTGCCCAAGGCTTTGTAGTAGAGGGGGAAGTCGCCTATCCGCACCGGCacgac              >  1:100037/1‑150 (MQ=255)
                                                                                                                                      gTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCGTATTGGTCGCGGTAACCGTGCCCAAGGCTTTGTAGTAGAGGGGGGAGTCGCCTATCCGCACCGGCacgac              >  2:157242/1‑150 (MQ=255)
                                                                                                                                         GCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCGTATTGGTCGCGGTAACCGTGCCCAAGGCTTTGTAGTaga                                             >  1:732814‑M1/1‑116 (MQ=255)
                                                                                                                                         GCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCGTATTGGTCGCGGTAACCGTGCCCAAGGCTTTGTAGTaga                                             <  2:732814‑M1/116‑1 (MQ=255)
                                                                                                                                          CCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCGTATTGGTCGCGGTAACCGTGCCCAAGGCTTTGTAGTagag                                            <  2:1082317‑M1/116‑1 (MQ=255)
                                                                                                                                          CCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCGTATTGGTCGCGGTAACCGTGCCCAAGGCTTTGTAGTagag                                            >  1:1082317‑M1/1‑116 (MQ=255)
                                                                                                                                           catc‑cTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCGTATTg                                                                               <  1:878611/77‑1 (MQ=255)
                                                                                                                                           CAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCGTATTg                                                                               <  2:878611‑M1/80‑1 (MQ=255)
                                                                                                                                              C‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCGTATTGGTCGCGGTAACCGTg                                                                <  2:381135‑M1/92‑1 (MQ=255)
                                                                                                                                              C‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCGTATTGGTCGCGGTAACCGTg                                                                >  1:381135‑M1/1‑92 (MQ=255)
                                                                                                                                                   gACGCGCTGGCCTTCCTTGAAGTGGATTCTCACCAGTTCGCCAGCTACGCGGCTGCGCATATTGATCGTATTGGTCGCGGTAAACGTGCCCAAGGTGTTGTAGTAGAGGGGGAAGTCGCCTATCCGCACCGGTATGACGCGTACGGGTAc  <  2:141104/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TCTTCAGCGTACAGGCCTTTATAGCGGGCCAGGTCGACCTGAGCGTTCTTCAACTGTGCCTGGTTCTGCGCCAAGGTGCCTTCGGCCTGCTGCAGGGCGATGCGATAAGGGCGCGGGTCGATTTCGGCCAGCAGGTCGCCAGC‑CTTGACGCGCTGGCCTTCCTTGAAGTGGATTTTCACCAGTTCGCCAGCCACGCGGCTGCGCACATTGACCGTATTGGTCGCGGTAACCGTGCCCAAGGCTTTGTAGTAGAGGGGGAAGTCGCCTATCCGCACCGGCACGACGCGTACGGGTAC  >  NC_002947/4073019‑4073314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑