| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,128,188 | C→G | 9.3% | A171A (GCC→GCG) | htrG → | signal transduction protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,128,188 | 0 | C | G | 9.3% | 159.8 / 7.6 | 54 | A171A (GCC→GCG) | htrG | signal transduction protein |
| Reads supporting (aligned to +/- strand): ref base C (28/21); new base G (2/3); total (30/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-01 | |||||||||||
CATCGACGACAGCTGGAAGAACCGCGTACAGGGCATGCAGGAAACGCTTGATTCGCGCAAGCAGCTGATTGATGAGTTGGAGGCACGCAACAAGGCACTGAACGAGCAGCTCGATCAGAGCCAGTCGGACCTGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCGGTGCAGGCTTGCTGGCGGGGCTGATCCTGCCGGCACTGACCCGTGGGCGCAAGAAGAACGATCGCTGGTTCTGAGCTCGGCAAG > NC_002947/4128041‑4128336 | cATCGACGACAGCTGGAAGAACCGCGTACAGGGCATGCAGGAAACGCTTGATTCGCGCAAGCAGCTGATTGATGAGTTGGAGGCACGCAACAAGGCACTGAACGAGCAGCTCGATCAGAGCCAGTCGGACCTGCGCGATACCCAGGCCCg > 2:1342260/1‑150 (MQ=255) aGCTGGAAGAACCGCGTACAGGGCATGCAGGAAACGCTTGATTCGCGCAAGCAGCTGATTGATGAGTTGGAGGCACGCAACAAGGCACTGAACGAGCAGCTCGATCAGAGCCAGTCGGACCTGCGCGATACCCAGGCCCGCCTGGGGGAt < 1:1074275/150‑1 (MQ=255) cTGGAAGAACCGCGTACAGGGCATGCAGGAAACGCTTGATTCGCGCAAGCAGCTGATTGATGAGTTGGAGGCACGCAACAAGGCACTGAACGAGCAGCTCGATCAGAGCCAGTCGGACCTGCGCGATACCCAGGCGGGCCTgggtatcgc < 2:1250689/150‑7 (MQ=255) ggAAGAACCGCGTACAGGGCATGCAGGAAACGCTTGATTCGCGCAAGCAGCTGATTGATGAGTTGGAGGCACGCAACAAGGCACTGAACGAGCAGCTCGATCAGAGCCAGTCGGACCTGCGCGATACCCAGGCCCGCCTGGGGGATgaga > 2:1070931/1‑150 (MQ=255) ccGCGTACAGGGCATGCAGGAAACGCTTGATTCGCGCAAGCAGCTGATTGATGAGTTGGAGGCACGCAACAAGGCACTGAACGAGCAGCTCGATCAGAGCCAGTCGGACCTGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCa > 1:483497/1‑150 (MQ=255) tACAGGGCATGCAGGAAACGCTTGATTCGCGCAAGCAGCTGATTGATGAGTGGGAGGCACGCAACAAGGCACTGAACGAGCAGCTCGAGCAGAGCCAGTCGGACCTGCGCGATACCCAGGCCCGCCGGGGGGGTGAGAACAAGCAGGtgg > 2:1235708/1‑149 (MQ=255) gggCATGCAGGAAACGCTTGATTCGCGCAAGCAGCTGATTGATGAGTTGGAGGCACGCAACAAGGCACTGAACGAGCAGCTCGATCAGAGCCAGTCGGACCTGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAg > 1:103964/1‑142 (MQ=255) gggCATGCAGGAAACGCTTGATTCGCGCAAGCAGCTGATTGATGAGTTGGAGGCACGCAACAAGGCACTGAACGAGCAGCTCGATCAGAGCCAGTCGGACCTGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAg < 2:103964/142‑1 (MQ=255) cTTGATTCGCGCAAGCAGCTGATTGATGAGTTGGAGGCACGCAACAAGGCACTGAACGAGCAGCTCGATCAGAGCCAGTCGGACCTGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTAt < 1:151758/150‑1 (MQ=255) aTTCGCGCAAGCAGCTGATTGATGAGTTGGAGGCACGCAACAAGGCACTGAACCAGTAGCTTATACAGAGCGATTCGGACCTGCGCGATACCCCGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATggcg < 1:324185/150‑1 (MQ=255) ttCGCGCAAGCAGCTGATTGATGAGTTGGAGGCACGCAACAAGGCACTGAACGAGCAGCTCGATCAGAGCCAGTCGGACCTGCGCGATACCCAGGCCCGCCT‑GGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATggcggc > 2:53247/1‑150 (MQ=255) gcgcAAGCAGCTGATTGATGAGTTGGAGGCACGCAACAAGGCACTGAACGAGCAGCTCGATCAGAGCCAGTCGGACCTGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGgcag > 1:928707/1‑150 (MQ=255) gcTGATTGATGAGTTGGAGGCACGCAACAAGGCACTGAACGAGCAGCTCGATCAGAGCCAGTCGGACCTGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCgg > 2:978001/1‑150 (MQ=255) attgatGAGTTGGAGGCACGCAACAAGGCACTGAACGAGCAGCTCGATCAGAGCCAGTCGGACCTGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCGGTGCa < 2:1090628/150‑1 (MQ=255) gatgaGTTGGAGGCACGCAACAAGGCACTGAACGAGCAGCTCGATCAGAGCCAGTCGGACCTGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCg < 2:1282691/142‑1 (MQ=255) gatgaGTTGGAGGCACGCAACAAGGCACTGAACGAGCAGCTCGATCAGAGCCAGTCGGACCTGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCg > 1:1282691/1‑142 (MQ=255) gTTGGAGGCACGCAACAAGGCACTGAACGAGCAGCTCGATCAGAGCCAGTCGGACCTGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAGGTgatgat > 2:1254147/1‑105 (MQ=255) gTTGGAGGCACGCAACAAGGCACTGAACGAGCAGCTCGATCAGAGCCAGTCGGACCTGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAGGTgatgat < 1:1254147/105‑1 (MQ=255) gAGGCACGCAACAAGGCACTGAACGAGCAGCTCGATCAGAGCCAGTCGGACCTGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCGGTGCAGGCTTGCTGGCg < 1:289210/150‑1 (MQ=255) aCGCAACAAGGCACTGAACGAGCAGCTCGATCAGAGCCAGTCGGACCTGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCGGTGCAGGCTTGCTGGCGGGGCt > 1:275964/1‑150 (MQ=255) aCGCAACAAGGCACTGAACGAGCAGCTCGATCAGAGCCAGTCGGACCTGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCGGTGCAGGCTTGCTGGCGGGGCt > 1:1036039/1‑150 (MQ=255) gCAACAAGGCACTGACAGAGCAGCTCGACCAGAGCCAGTCGGACCTGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACAGGGTGTATGGCGGCAGCATTGCCGGTGCAGGCTTGCTGGCGGGGCTGa < 1:1235708/150‑1 (MQ=255) aataaGGCACTGAACGAGCAGCTCGATCAGAGCCAGTCGGACCTGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCGGTGCAGGCTTGCTGGCGGGGCTGATc < 1:20944/147‑1 (MQ=255) aaGGCACTGAACGAGCAGCTCGATCAGAGCCAGTCGGACCTGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCGGTGCAGGCTTGCTGGCGGGGCTGATCCTg > 1:953826/1‑150 (MQ=255) tcTGATCGAGCTGCTCGTTCAGAGCCAGTCGGACCTGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCGGTGCAGGCTTGCTGGCGGGGCTGATCCTGCCGGc > 2:621820/2‑150 (MQ=255) gcagcTCGATCAGAGCCAGTCGGACCTGCGCGATACCCAGGCCCGCCTGGGGGATga > 1:716329/1‑57 (MQ=255) gcagcTCGATCAGAGCCAGTCGGACCTGCGCGATACCCAGGCCCGCCTGGGGGATga < 2:716329/57‑1 (MQ=255) gcTCGATCAGAGCCAGTCGGACCTGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCGGTGCAGGCTTGCTGGCGGGGCTGATCCTGCCGGCACTGACCCGTgg < 2:1011380/150‑1 (MQ=255) tCGATCAGAGCCAGTCGGACCTGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCGGTGCAGGCTTGCGGACGGGGCGGATCCTGTCGGCAATGAACCGCgggg > 2:944854/1‑149 (MQ=255) cGATCAGAGCCAGTCGGACCTGCGCGATACCCAGGCCCGCCT‑GGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCGGTGCAGGCTTGCTGGCGGGGCTGATCCTGCCGGCACTGACCCGTGGgcgc < 1:53247/150‑1 (MQ=255) gagCCAGTCGGACCTGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCGGTGCAGGCTTGCTGGCGGGGCTGATCCTGCCGGCACTGACCCGTGGGCGCaagaa > 2:743175/1‑150 (MQ=255) gagCCAGTCGGACCTGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCGGTGCAGGCTTGCTGGCGGGGCTGATCCTGCCGGCACTGACCCGTGGGCGCaagaa > 2:751194/1‑150 (MQ=255) agCCAGTCGGACCTGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCGGTGCAGGCTTGCTGGCGGGGCTGATCCTGCCGGCACTGACCCGTGGGCGCaagaag > 1:837335/1‑150 (MQ=255) agCCAGTCGGACCTGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCGGTGCAGGCTTGCTGGCGGGGCTGATCCTGCCGGCACTGACCCGGGGGCGCaagaag > 1:487669/1‑150 (MQ=255) ccAGTCGGACCTGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCGGTGCAGGCTTGCTGGCGGGGCTGATCCTGCCGGCACTGACCCGTGGGCGCAAgaagaa < 1:1147514/150‑1 (MQ=255) ccAGTCGGACCTGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCGGTGCAGGCTTGCTGGCGGGGCTGATCCTGCCGGCACTGACCCGTGGGCGCAAgaagaa > 1:825604/1‑150 (MQ=255) ccAGTCGGACCTGCGCGATACCAACGGGTTGCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCGGTGCAGGCTTGCTGGCGGGGCTGATCCTGCCGGCACTGACCCGTGGGCGCAAgaagaa < 1:1070931/150‑1 (MQ=255) aCCTGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGcc < 2:1184984/83‑1 (MQ=255) aCCTGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGcc > 1:1184984/1‑83 (MQ=255) aCCTGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTACGGCGGCAGCCTTGGCCGTGCAGGCTTGCTGGCGGGGCTGGCCCTGCCGGCACTGAGCCGGGTGCGCAATAAGAACGATCGCt < 2:873964/150‑1 (MQ=255) tGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGgt > 1:288543/1‑61 (MQ=255) tGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGgt < 2:288543/61‑1 (MQ=255) tGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCGGTGCAGGCTTGCTGGCGGGGCTGATCCTGCCGGCACTGACCCGTGGGCGCAAGAAGAACGATCGCTGGt > 2:246255/1‑150 (MQ=255) tACCCAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCGGTGCAGGCTTGCTGGCGGGGCTGATCCTGCCGGCACTGACCCGTGGGCGCAAGAAGAACGATCGCTGGTTCTGAGc > 2:1297275/1‑150 (MQ=255) cccccAGGCGGGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCGGTGCAGGCTTGCTGGCGGGGc > 2:469602/3‑95 (MQ=255) cccccAGGCGGGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCGGTGCAGGCTTGCTGGCGGGGc < 1:469602/93‑1 (MQ=255) ccccAGGCGGGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCGGTGCAGGCTTGCTGGCGGGGCTGATCCTGCCGGCACTGACCCGTGGGCGCAAGAAGAACGATCGCTGGTTCTGAGCt > 2:217571/2‑150 (MQ=255) ccAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCGGTGCAGGCTTGCTGGCGGGGCTGATCCTGCCGGCACTGACCCGTGGGCGCAAGAAGAACGATCGCTGGTTCTGAGCTCg > 1:802465/1‑150 (MQ=255) ccAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCGGTGCAGGCTTGCTGGCGGGGCTGATCCTGCCGGCACTGACCCGTGGGCGCAAGAAGAACGATCGCTGGTTCTGAGCTCg < 1:1342260/150‑1 (MQ=255) ccAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCGGTGCAGGCTTGCTGGCGGGGCTGATCCTGCCGGCACTGACCCGTGGGCGCAAGAAGAACGATCGCTAGTTc > 1:118585/1‑142 (MQ=255) ccAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCGGTGCAGGCTTGCTGGCGGGGCTGATCCTGCCGGCACTGACCCGTGGGCGCAAGAAGAACGATCGCTAGTTc < 2:118585/142‑1 (MQ=255) ggCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCGGTGCAGGCTTGCTGGCGGGGCTGATCCTGCCGGCACTGACCCGTg > 2:238197/1‑113 (MQ=255) ggCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCGGTGCAGGCTTGCTGGCGGGGCTGATCCTGCCGGCACTGACCCGTg < 1:238197/113‑1 (MQ=255) gCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCGGTGCAGGCTTGCTGGCGGGGCTGATCCTGCCGGCACTGACCCGTGGGCGCAAGAAGAACGATCGCTGGTTCTGAGCTCGGCaa < 2:1036039/150‑1 (MQ=255) cccGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCGGTGCAGGCTTGCTGGCGGGGCTGATCCTGCCGGCACTGACCCGTGGGCGCAAGAAGAACGATCGCTGGTTCTGAGCTCGGCAAg < 1:621820/150‑1 (MQ=255) | CATCGACGACAGCTGGAAGAACCGCGTACAGGGCATGCAGGAAACGCTTGATTCGCGCAAGCAGCTGATTGATGAGTTGGAGGCACGCAACAAGGCACTGAACGAGCAGCTCGATCAGAGCCAGTCGGACCTGCGCGATACCCAGGCCCGCCTGGGGGATGAGAACAAGCAGGTGATGATGCGCTACATGGTGTATGGCGGCAGCATTGCCGGTGCAGGCTTGCTGGCGGGGCTGATCCTGCCGGCACTGACCCGTGGGCGCAAGAAGAACGATCGCTGGTTCTGAGCTCGGCAAG > NC_002947/4128041‑4128336 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |