| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,160,706 | G→C | 9.7% | intergenic (+28/+93) | PP_3662 → / ← PP_3663 | hypothetical protein/GGDEF domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,160,706 | 0 | G | C | 9.7% | 185.7 / 9.2 | 62 | intergenic (+28/+93) | PP_3662/PP_3663 | hypothetical protein/GGDEF domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base G (27/29); new base C (3/3); total (30/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01 | |||||||||||
TCCACGGTGACAGCGCCGTACTGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAACCACCGCCCGCTCGAAAAACCGAATGCTGTCGCGCCCCC > NC_002947/4160557‑4160854 | tCCACGGTGACAGCGCCGTACTGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATgg < 1:735145/150‑1 (MQ=255) aCAGCGCCGTACTGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATCCATGCCCGTc < 1:405200/150‑1 (MQ=255) cgcCGTACTGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAAc < 2:1409515/150‑1 (MQ=255) gcCGTACTGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAAcg > 1:1213452/1‑150 (MQ=255) aCTGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCgggg < 1:850060/150‑1 (MQ=255) tGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGc < 2:1213452/150‑1 (MQ=255) gACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCCGACCCGCTGCTTTACTGAGGACGGGCCTGGATGCCCGTCGAACGCGCGGGTCg > 2:1078397/1‑150 (MQ=255) gACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCCGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCg > 2:1078384/1‑150 (MQ=255) aCCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCgg < 2:1227228/150‑1 (MQ=255) aCCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCgg < 1:349314/150‑1 (MQ=255) cccGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCggg < 1:908429/150‑1 (MQ=255) gCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGa > 2:616114/1‑150 (MQ=255) gCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGTTAAAGCTGGCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCCGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGa < 1:1078397/150‑1 (MQ=255) gCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCCGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGa < 1:1078384/150‑1 (MQ=255) gcAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCCTGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTAc < 1:1014305/150‑1 (MQ=255) cAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACg > 2:228813/1‑150 (MQ=255) aGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACgg < 1:735633/150‑1 (MQ=255) aGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACgg > 2:735633/1‑150 (MQ=255) aTTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATCCATGCCCGTc < 2:1019547/110‑1 (MQ=255) aTTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATCCATGCCCGTc > 1:1019547/1‑110 (MQ=255) ttCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCAc < 1:475525/150‑1 (MQ=255) ccAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATgg < 1:1321598/150‑1 (MQ=255) cAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGCATGTACCGTGCACAACGGGGGCAACCTGACCCGCTGCTGGACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATggg > 1:958433/1‑150 (MQ=255) gCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGtt < 1:945105/150‑1 (MQ=255) aCCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGa > 1:1249574/1‑120 (MQ=255) aCCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGa < 2:1249574/120‑1 (MQ=255) aCCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATCCATGCCCGTc < 2:830197/96‑1 (MQ=255) aCCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATCCATGCCCGTc > 1:830197/1‑96 (MQ=255) cGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACAt < 2:958433/150‑1 (MQ=255) tGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGaaa < 1:616114/150‑1 (MQ=255) gCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTc > 1:251790/1‑150 (MQ=255) cTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGGCCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTcc < 1:606912/150‑1 (MQ=255) ccGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGccc < 1:61064/78‑1 (MQ=255) ccGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGccc > 2:61064/1‑78 (MQ=255) ggcgcggcgTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAg < 1:841020/150‑1 (MQ=255) cgcggcgTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGt > 1:1091952/1‑150 (MQ=255) aTGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCt > 2:806821/1‑150 (MQ=255) cGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACgggca > 2:83700/1‑83 (MQ=255) cGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACgggca < 1:83700/85‑3 (MQ=255) cGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTa < 2:434753/113‑1 (MQ=255) cGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGGTa > 1:434753/1‑113 (MQ=255) cTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGa < 1:1332087/150‑1 (MQ=255) cTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGa < 1:228813/150‑1 (MQ=255) tATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAg > 1:499380/1‑150 (MQ=255) tATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAg > 1:564209/1‑150 (MQ=255) gTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATgg < 1:242366/98‑1 (MQ=255) gTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATgg > 2:242366/1‑98 (MQ=255) tGGCAACCTGACCCGCTGCTTTACTGAGGACGGGTATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTAc < 1:1069478/82‑1 (MQ=255) tGGCAACCTGACCCGCTGCTTTACTGAGGACGGGTATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTAc > 2:1069478/1‑82 (MQ=255) aaCCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAg < 1:344341/118‑1 (MQ=255) aaCCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAg > 2:344341/1‑118 (MQ=255) ccTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGACAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAACCAccgcc > 2:452672/1‑150 (MQ=255) cTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAACCAccgccc > 1:1200010/1‑150 (MQ=255) cGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAACCACCGCCCGCTCGa > 1:1414983/1‑150 (MQ=255) ctgctTTACTGAGGACGGGCATGGATGACGGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAACCACCGCCCGCTCGaaa > 1:38206/1‑150 (MQ=255) cTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAACCACCGCCCGCTCGAAAAACCGAAt > 2:1158333/1‑150 (MQ=255) tGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAac > 1:1198234/1‑125 (MQ=255) tGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAac < 2:1198234/125‑1 (MQ=255) gACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATg < 1:706348/77‑1 (MQ=255) gACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATg > 2:706348/1‑77 (MQ=255) gACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGa < 1:1232303/78‑1 (MQ=255) gACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGa > 2:1232303/1‑78 (MQ=255) aCGGGCATCCATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAACCACCGCCCGCTCGAAAAACCGAATGCTGTc > 2:1112784/1‑150 (MQ=255) ggATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAACCACCGCCCGCTCGAAAAACCGAATGCTGTCGCGccccc < 2:1091952/150‑1 (MQ=255) | TCCACGGTGACAGCGCCGTACTGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAACCACCGCCCGCTCGAAAAACCGAATGCTGTCGCGCCCCC > NC_002947/4160557‑4160854 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |