Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,162,152 G→A 15.2% intergenic (‑52/+25) PP_3663 ← / ← pssA GGDEF domain‑containing protein/CDP‑diacylglycerol‑‑serine O‑phosphatidyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,162,1520GA15.2% 105.1 / 11.5 46intergenic (‑52/+25)PP_3663/pssAGGDEF domain‑containing protein/CDP‑diacylglycerol‑‑serine O‑phosphatidyltransferase
Reads supporting (aligned to +/- strand):  ref base G (21/18);  new base A (4/3);  total (25/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01

ATGCGATCGTGATCACGTTCAGAGGGGCCTTGCACTGTTGCCTGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCACTTGCTTGGGATGTTCGGCCTTCTCCCCCAGCGCCTCTGCCGAACCGATTCTCGGCGCAA  >  NC_002947/4162009‑4162301
                                                                                                                                               |                                                                                                                                                     
aTGCGATCGTGATCACGTTCAGAGGGGCCTTGCACTGTTGCCTGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACa                                                                                                                                                 <  1:987646/150‑1 (MQ=255)
aTGCGATCGTGATCACGTTCAGAGGGGCCTTGCACTGTTGCCTGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCCGGCAAGCCAGCGACa                                                                                                                                                 <  1:1201810/150‑1 (MQ=255)
 tGCGATCGTGATCACGTTCAGAGGGGCCTTGCACTGTTGCCTGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAg                                                                                                                                                <  1:124878/150‑1 (MQ=255)
   cGATCGTGATCACGTTCAGAGGGGCCTTGCACTGTTGCCTGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAggg                                                                                                                                              <  2:226538/150‑1 (MQ=255)
      tCGTGATCACGTTCAGAGGGGCCTTGCACTGTTGCCTGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAg                                                                                                                                                >  1:1009948/1‑145 (MQ=255)
      tCGTGATCACGTTCAGAGGGGCCTTGCACTGTTGCCTGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAg                                                                                                                                                <  2:1009948/145‑1 (MQ=255)
        gTGATCACGTTCAGAGGGGCCTTGCGCTGTTGCCTGGAGATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATGTCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCGGGCTTGCCGGCTACAGGGtgcgt                                                                                                                                         >  2:407606/1‑146 (MQ=255)
                           ccTTGCACTGTTGCCTGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCAc                                                                                                                      >  1:999228/1‑150 (MQ=255)
                               gCACTGTTGCCTGCACATTGTAATAAAGATCCGGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGc                                                                                                                  >  1:37563/1‑150 (MQ=255)
                                    agatctggCACATTGCAATCAGGTCGCTGACAAAGTGCCTTTGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGAt                                                                                                             <  2:1373404/143‑1 (MQ=255)
                                     ttGCCTGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATc                                                                                                            >  2:1016737/1‑150 (MQ=255)
                                       gCCTGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCaa                                                                                                          >  1:742885/1‑150 (MQ=255)
                                        ccTGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCCGGCAAGCCAGCGACAg                                                                                                                                                <  2:504548/111‑1 (MQ=255)
                                        ccTGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCCGGCAAGCCAGCGACAg                                                                                                                                                >  1:504548/1‑111 (MQ=255)
                                           gCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCagatcg                                                                                                      >  1:1373404/1‑145 (MQ=255)
                                             acaTTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTc                                                                                                    <  1:625133/150‑1 (MQ=255)
                                               aTTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGAt                                                                                                             >  1:1225504/1‑139 (MQ=255)
                                               aTTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGAt                                                                                                             <  2:1225504/139‑1 (MQ=255)
                                                ttGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGt                                                                                                                                             >  1:856904/1‑106 (MQ=255)
                                                ttGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGt                                                                                                                                             <  2:856904/106‑1 (MQ=255)
                                                          gCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAg                                                                                       <  2:802667/150‑1 (MQ=255)
                                                               tGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCaa                                                                                  >  1:1115746/1‑150 (MQ=255)
                                                                   aaaGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGt                                                                                                                                             >  1:1188336/1‑87 (MQ=255)
                                                                   aaaGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGt                                                                                                                                             <  2:1188336/87‑1 (MQ=255)
                                                                         ccGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGACCCTGTCGCCGGCAAGCCAGCGACa                                                                                                                                                 <  1:512919/77‑1 (MQ=25)
                                                                         ccGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGACCCTGTCGCCGGCAAGCCAGCGACa                                                                                                                                                 >  2:512919/1‑77 (MQ=25)
                                                                                 agcaATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCg                                                                                                                                                       >  2:182374/1‑63 (MQ=255)
                                                                                 agcaATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCg                                                                                                                                                       <  1:182374/63‑1 (MQ=255)
                                                                                 agcaATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCGGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTgcg                                                                >  2:1390346/1‑150 (MQ=255)
                                                                                 agcaATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTgcg                                                                <  2:742885/150‑1 (MQ=255)
                                                                                   caATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAgg                                                                                                                                               <  2:1142414/69‑1 (MQ=255)
                                                                                   caATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAgg                                                                                                                                               >  1:1142414/1‑69 (MQ=255)
                                                                                      tatCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCaga                                                                                                                          <  2:697432/87‑1 (MQ=255)
                                                                                      tatCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCaga                                                                                                                          >  1:697432/1‑87 (MQ=255)
                                                                                       atCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCActtg                                                          <  2:377126/150‑1 (MQ=255)
                                                                                                  aTCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTAAGTCCAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCACTTGCTTGGGATGtt                                               >  2:194794/1‑150 (MQ=255)
                                                                                                       tGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCACTTGCTTGGGATGTTCGGcc                                          <  2:999228/150‑1 (MQ=255)
                                                                                                           cgGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCACTTGCTTGGGATGTTCGGCCTtct                                      >  2:187641/1‑150 (MQ=255)
                                                                                                                   accacTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCACTTGCTTGGGATGTTCGGCCTTCTCCCCCAgc                              <  1:1016737/150‑1 (MQ=255)
                                                                                                                            gCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCACTTGCTTGGGATGTTCGGCCTTCTCCCCCAGCGCCTCTGcc                     >  1:1406969/1‑150 (MQ=255)
                                                                                                                            gCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCACTTGCTTGGGATGTTCGGCCTTCTCCCCCAGCGCCTCTGcc                     >  1:1177582/1‑150 (MQ=255)
                                                                                                                              ccTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTc                                                                                                    <  2:1267108/69‑1 (MQ=255)
                                                                                                                              ccTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTc                                                                                                    >  1:1267108/1‑69 (MQ=255)
                                                                                                                               cTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCACTTGCTTGGGATGTTCGGCCTTCTCCCCCAGCGCCTCTGCCGaa                  >  2:180877/1‑150 (MQ=255)
                                                                                                                               cTGTCGCCGGCAAGCCAGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCACTTGCTTGGGATGTTCGACCTTCTCCCCCAGCGCCTCTGCCGaa                  >  2:39623/1‑150 (MQ=255)
                                                                                                                               cTGTCGCCGGCAAGCCAGCGACAGGGCCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCACTTGCTTGGGATGTTCGGCCTTCTCCCCCAGGGCCTCTGCCGaa                  >  2:441288/1‑150 (MQ=255)
                                                                                                                                tGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCACTTGCTTGGGATGTTCGGCCTTCTCCCCCAGCGCCTCTGCCGAAc                 >  1:1159259/1‑150 (MQ=255)
                                                                                                                                    gCTGGCTTGCCGGCGACAGGCTCCGTACAGGCGCTGTCAGAGCTCCCGCTTGAGCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCACTTGCTTGGGATGTTCGGCCTTCTCCCCCAGCGCCTCTGCCGAACCGAt             <  1:1161509/150‑1 (MQ=255)
                                                                                                                                               ggCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCACTTGCTTGGGATGTTCGGCCTTCTCCCCCAGCGCCTCTGCCGAACCGATTCTCGGCGCac  >  2:1223045/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
ATGCGATCGTGATCACGTTCAGAGGGGCCTTGCACTGTTGCCTGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCACTTGCTTGGGATGTTCGGCCTTCTCCCCCAGCGCCTCTGCCGAACCGATTCTCGGCGCAA  >  NC_002947/4162009‑4162301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑