Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,208,705 A→T 7.3% Q205L (CAG→CTG)  PP_3692 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,208,7050AT 7.3% 204.8 / 6.4 69Q205L (CAG→CTG) PP_3692hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (33/30);  new base T (3/2);  total (37/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01

TGCGGGTCTCGCTCCAAGGACGAGATACGGAGCAACGTATTCGACCCTAACCATACGGACTGCCGGGCTCAATTCAAGGTGTCGATTGACGGCAAATCCGTTGATGTCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTCATCGCCACTGCCGATGCCGTCAACACAGAGTTGACAAGGGAGAAGGAAAGGC  >  NC_002947/4208557‑4208851
                                                                                                                                                    |                                                                                                                                                  
tGCGGGTCTCGCTCCAAGGACGAGATACGGAGCAACGTATTCGACCCTAACCATACGGACTGCCGGGCTCAATTCAAGGTGTCGATTGACGGCAAATCCGGGGATTTCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAg                                                                                                                                                   >  2:1228935/1‑150 (MQ=255)
  cGGGTCTCGCTCCAAGGACGAGATACGGAGCAACGTATTCGACCCTAACCATACGGACTGCCGGGCTCAATTCAAGGTGTCGATTGACGGCAAATCCGTTGATGTCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGgc                                                                                                                                                 >  2:1283480/1‑150 (MQ=255)
   gggTCTCGCTCCAAGGACGAGATACGGAGCAACGTATTCGACCCTAACCATACGGACTGCCGGGCTCAATTCAAGGTGTCGATTGACGGCAAATCCGTTGATGTCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCCGgcg                                                                                                                                                >  1:553910/1‑150 (MQ=255)
    ggTCTCGCTCCAAGGACGAGATACGGAGCAACGTATTCGACCCTAACCATACGGACTGCCGGGCTCAATTCAAGGTGTCGATTGACGGCAAATCCGTTGATGTCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGgcgc                                                                                                                                               >  2:1057344/1‑150 (MQ=255)
        tcGCTCCAAGGACGAGATACGGAGCAACGTATTCGACCCTAACCATACGGACTGCCGGGCTCAATTCAAGGTGTCGATTGACGGCAAATCCGTTGATGTCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAgt                                                                                                                                           >  2:481906/1‑150 (MQ=255)
            tCCAAGGACGAGATACGGAGCAACGTATTCGACCCTAACCATACGGACTGCCGGGCTCAATTCAAGGTGTCGATTGACGGCAAATCCGTTGATGTCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTc                                                                                                                                       <  2:851919/150‑1 (MQ=255)
                aGGACGAGATACGGAGCAACGTATTCGACCCTAACCATACGGACTGCCGGGCTCAATTCAAGGTGTCGATTGACGGCAAATCCGTTGATGTCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATa                                                                                                                                   <  2:1139180/150‑1 (MQ=255)
                aGGACGAGATACGGAGCAACGTATTCGACCCTAACCATACGGACTGCCGGGCTCAATTCAAGGTGTCGATTGACGGCAAATCCGTTGATGTCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATa                                                                                                                                   <  1:1057344/150‑1 (MQ=255)
                    cGAGATACGGAGCAACGTATTCGACCCTAACCATACGGACTGCCGGGCTCAATTCAAGGTGTCGATTGACGGCAAATCCGTTGATGTCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTaa                                                                                                                               <  1:299736/150‑1 (MQ=255)
                     gagaTACGGAGCAACGTATTCGACCCTAACCATACGGACTGCCGGGCTCAATTCAAGGTGTCGATTGACGGCAAATCCGTTGATGTCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTaaa                                                                                                                              <  1:1283480/150‑1 (MQ=255)
                                 aaCGTATTCGACCCTAACCATACGGACTGCCGGGCTCAATTCAAGGTGTCGATTGACGGCAAATCCGTTGATGTCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCaa                                                                                                                  >  1:988965/1‑150 (MQ=255)
                                 aaCGTATTCGACCCTAACCATACGGACTGCCGGGCTCAATTCAAGGTGTCGATTGACGGCAAATCCGTTGATGTCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCaa                                                                                                                  <  2:553910/150‑1 (MQ=255)
                                   cGTATTCGACCCTAACCATACGGACTGCCGGGCTCAATTCAAGGTGTCGATTGACGGCAAATCCGTTGATGTCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATa                                                                                                                >  1:1135048/1‑150 (MQ=255)
                                        tCGACCCTAACCATACGGACTGCCGGGCTCAATTCAAGGTGTCGATTGACGGCAAATCCGTTGATGTCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATgg                                                                                                           <  2:988965/150‑1 (MQ=255)
                                                aaCCATACGGACTGCCGGGCTCAATTCAAGGTGTCGATTGACGGCAAATCCGTTGATGTCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACt                                                                                                   >  2:1133471/1‑150 (MQ=255)
                                                          aCTGCCGGGCTCAATTCAAGGTGTCGATTGACGGCAAATCCGTTGATGTCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCt                                                                                         <  2:1321326/150‑1 (MQ=255)
                                                           cTGCCGGGCTCAATTTAAGGTGTCGATTGACGGCAAATCCGTTGATGTCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTa                                                                                        <  2:658377/150‑1 (MQ=255)
                                                           cTGCCGGGCTCAATTCAAGGTGTCGATTGACGGCAAATCCGTTGATGTCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCTATAGTAAAGCCGCTCAGCAATTCATGGGGCGAACTGAAGCGTGCTa                                                                                        <  2:163751/150‑1 (MQ=255)
                                                                                   gATTGACGGCAAATCCGTTGATGTCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTtgt                                                                <  1:1051238/150‑1 (MQ=255)
                                                                                   gATTGACGGCAAATCCGTTGATGTCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTtgt                                                                >  1:1293567/1‑150 (MQ=255)
                                                                                   gATTGACGGCAAATCCGTTGATGTCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTtgt                                                                <  1:1192152/150‑1 (MQ=255)
                                                                                    aTTGACGGCAAATCCGTTGATGTCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCa                                                                                                                      <  2:1024166/95‑1 (MQ=255)
                                                                                    aTTGACGGCAAATCCGTTGATGTCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCa                                                                                                                      >  1:1024166/1‑95 (MQ=255)
                                                                                        aCGGCAAATCCGTTGATGTCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCAAAGTCGCATGAAATTTGTGTCgg                                                           >  1:183614/1‑150 (MQ=255)
                                                                                         cGGCAAATCCGTTGATGTCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGc                                                          >  1:1214085/1‑150 (MQ=255)
                                                                                          ggCAAATCCGTTGATGTCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGCACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCa                                                         >  2:1325239/1‑150 (MQ=255)
                                                                                           gCAAATCCGTTGATGTCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCAc                                                        >  1:1139147/1‑150 (MQ=255)
                                                                                             aaaTCCGTTGATGTCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTc                                                      >  1:766913/1‑150 (MQ=255)
                                                                                                        tGTCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTAATCGCCACTGc                                           <  1:481906/150‑1 (MQ=255)
                                                                                                          tCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGc                                                                                   >  2:1334216/1‑108 (MQ=255)
                                                                                                          tCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGc                                                                                   <  1:1334216/108‑1 (MQ=255)
                                                                                                          tCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTCATCGCCACTGCCg                                         >  2:769798/1‑150 (MQ=255)
                                                                                                          tCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTCATCGCCACTGCCg                                         >  2:712460/1‑150 (MQ=255)
                                                                                                          tCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTCATCGCCACTGCCg                                         >  1:1398261/1‑150 (MQ=255)
                                                                                                            cACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTCATCGCCACTGCCGAt                                       >  1:685568/1‑150 (MQ=255)
                                                                                                             aCTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCa                                                         >  2:76132/1‑131 (MQ=255)
                                                                                                             aCTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCa                                                         <  1:76132/131‑1 (MQ=255)
                                                                                                             aCTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTCATCGCCACTGCCg                                         >  1:936458/1‑147 (MQ=255)
                                                                                                             aCTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTCATCGCCACTGCCg                                         <  2:936458/147‑1 (MQ=255)
                                                                                                              cTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTCATCGCCACTGCCGATGc                                     >  2:1211126/1‑150 (MQ=255)
                                                                                                                 ggACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTCATCGCCACTGCCGa                                        >  1:283594/1‑144 (MQ=255)
                                                                                                                 ggACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTCATCGCCACTGCCGATGCCGt                                  >  2:149662/1‑150 (MQ=255)
                                                                                                                 ggACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTCATCGCCACTGACGa                                        <  2:283594/144‑1 (MQ=255)
                                                                                                                     aTCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTCATCGCCACTGCCGATGCCGTCAac                              >  2:1281362/1‑150 (MQ=255)
                                                                                                                        ccAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCa                                                                                                                      >  1:39075/1‑59 (MQ=255)
                                                                                                                        ccAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCa                                                                                                                      <  2:39075/59‑1 (MQ=255)
                                                                                                                          aaGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTCATCGCCACTGc                                           <  1:770168/132‑1 (MQ=255)
                                                                                                                          aaGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTCATCGCCACTGc                                           >  2:770168/1‑132 (MQ=255)
                                                                                                                            gCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTCATCGCCACTGCCGATGCCGTCAACACAGAGt                       <  2:1135048/150‑1 (MQ=255)
                                                                                                                               gCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTCATCGCCACTGCCGATGCCGTCAACACAGAGTTGa                    <  1:712460/150‑1 (MQ=255)
                                                                                                                                cccTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTCATCGCCACTGCCGATGCCGTCAACACAGAGTTGAc                   <  2:1398261/150‑1 (MQ=255)
                                                                                                                                   tCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCa                                                         >  1:1041718/1‑109 (MQ=255)
                                                                                                                                   tCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCa                                                         <  2:1041718/109‑1 (MQ=255)
                                                                                                                                    cacaAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTCATCGCCACTGCCGATGCCGTCAACACAGAGTTGACAAgg               <  1:1133471/150‑1 (MQ=255)
                                                                                                                                    cacaAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTCATCGCCACTGCCGATGCCGTCAACACAGAGTTGACAAgg               <  1:149662/150‑1 (MQ=255)
                                                                                                                                     acaAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCa                                                                          <  2:488626/90‑1 (MQ=255)
                                                                                                                                     acaAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCa                                                                          >  1:488626/1‑90 (MQ=255)
                                                                                                                                     acaAAGCGTTGCGACAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTCATCGCCACTGCCGTTGCCGTCAACACAGAGTTTACAAggg              >  1:298786/1‑150 (MQ=255)
                                                                                                                                       aaaGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTCATCGCCACTGCCGATGCCGTCAACACAGAGTTGACAAGGgag            >  1:642889/1‑150 (MQ=255)
                                                                                                                                        aaGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTCATCGCCACTGCCGATGCCGTCAACACAGAGTTGACAAGGgaga           >  2:283805/1‑150 (MQ=255)
                                                                                                                                        aaGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTCATCGCCACTGCCGATGCCGTCAACACAGAGTTGACAAGGgaga           <  2:183614/150‑1 (MQ=255)
                                                                                                                                         acacttGCGCCTGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTCATCGCCACTGCCGATGCCGTCAACACAGAGTTGACAAGGGAGaa          >  1:334492/5‑150 (MQ=255)
                                                                                                                                           acttGCGCCTGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTCATCGCCACTGCCGATGCCGTCAACACAGAGTTGACAAGGga             >  2:743358/3‑145 (MQ=255)
                                                                                                                                           acttGCGCCTGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTCATCGCCACTGCCGATGCCGTCAACACAGAGTTGACAAGGga             <  1:743358/143‑1 (MQ=255)
                                                                                                                                            cttGCGCCTGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTCATCGCCACTGCCGATGCCGTCAACACAGAGTTGACAAGGGAGaa          >  2:1414968/2‑147 (MQ=255)
                                                                                                                                            cttGCGCCTGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTCATCGCCACTGCCGATGCCGTCAACACAGAGTTGACAAGGGAGaa          <  1:1414968/146‑1 (MQ=255)
                                                                                                                                                cgcCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTCATCGCCACTGCAGATGCCGTAAACACAGAGTTGACGAGTGAGAAGGAAAgg   <  2:313463/150‑1 (MQ=255)
                                                                                                                                                 gcCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTCATCGCCACTGCCGATGCCGTCAACACAGAGTTGACAAGGGAGAAGGAAAGGc  <  2:685568/150‑1 (MQ=255)
                                                                                                                                                 gcCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTCATCGCCACTGCCGATGCCGTCAACACAGAGTTGACAAGGGAGAAGGAAAGGc  >  1:269089/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TGCGGGTCTCGCTCCAAGGACGAGATACGGAGCAACGTATTCGACCCTAACCATACGGACTGCCGGGCTCAATTCAAGGTGTCGATTGACGGCAAATCCGTTGATGTCCACTGGGACATCCCAAGCGGCCCTCACAAAGCGTTGCGCCAGGCGCAAGTGTTCGATAGTAAAGCCGCTCAGCAATACATGGGGCGAACTGAAGCGTGCTATGAGCCAAGTCGCATGAAATTTGTGTCGGCACTCATCGCCACTGCCGATGCCGTCAACACAGAGTTGACAAGGGAGAAGGAAAGGC  >  NC_002947/4208557‑4208851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑