Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,239,364 C→G 7.4% intergenic (+63/+70) catR → / ← PP_3717 HTH‑type transcriptional regulator/LuxR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,239,3640CG 7.4% 163.5 / 4.4 54intergenic (+63/+70)catR/PP_3717HTH‑type transcriptional regulator/LuxR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (24/26);  new base G (2/2);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.53e-01

CCCGATCATCCTCAGCCGGCGCAAGGGTGATGTGAGCCCGATCGTGCAGCGGTGCCTGGCGTTGATCGAGCAACAGGCCATATGAACACACCATGCCCACAGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAGGGCTCAAGACAGTCAATCTAAAATCATCGGCAAATGATCACCGCGTAACCAGTCGATAAACCGTGCAAACAACTCCGATTGCGAGTTGATC  >  NC_002947/4239217‑4239512
                                                                                                                                                   |                                                                                                                                                    
cccGATCATCCTCAGCCGGCGCAAGGGTGATGTGAGCCCGATCGTGCAGCGGTGCCTGGCGTTGATCGAGCAACAGGCCATATGAACACACCATGCCCACAGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCAGTATACCCAt                                                                                                                                                    <  1:744819/150‑1 (MQ=255)
         ccTCGGCCGGCGCGAGGGTGAGGTGAGGCCGATCGTGCAGCGGTGCCTGGGGGTGGGCGAGGAACAGGCCATATGAACGCACCATGCCCACAGGGGCTGTATAGCCCTGGCCTATTGGCTATGCCGGCGCCGTATACCCATCGGTATAgg                                                                                                                                           <  2:479133/150‑1 (MQ=255)
           tCAGCCGGCGCAAGGGTGATGTGAGCCCGATCGTGCAGCGGTGCCTGGCGTTGATCGAGCAACAGGCCATATGAACACACCATGCCCACAGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCGATGGGTATAcggg                                                                                                                                         <  1:1067988/150‑2 (MQ=255)
           tCAGCCGGCGCAAGGGTGATGTGAGCCCGATCGTGCAGCGGTGCCTGGCGTTGATCGAGCAACAGGCCATATGAACACACCATGCCCACAGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCGATGGGTATAcggg                                                                                                                                         >  2:703509/1‑149 (MQ=255)
           tCAGCCGGCGCAAGGGTGATGTGAGCCCGATCGTGCAGCGGTGCCTGGCGTTGATCGAGCAACAGGCCATATGAACACACCATGCCCACAGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGa                                                                                                                                         >  2:51786/1‑150 (MQ=255)
            cAGCCGGCGCAAGGGTGATGTGAGCCCGATCGTGCAGCGGTGCCTGGCGTTGATCGAGCAACAGGCCATATGAACACACCATGCCCACAGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCGATGGGtatacgggg                                                                                                                                        <  1:703509/150‑6 (MQ=255)
               ccGGCGCAAGGGTGATGTGAGCCCGATCGTGCAGCGGTGCCTGGCGTTGATCGAGCAACAGGCCATATGAACACACCATGCCCACAGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGtt                                                                                                                                     <  2:1073452/150‑1 (MQ=255)
                    gcAAGGGTGATGTGAGCCCGATCGTGCAGCGGTGCCTGGCGTTGATCGAGCAACAGGCCATATGAACACACCATGCCCACAGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCg                                                                                                                                                  >  1:1129680/1‑132 (MQ=255)
                    gcAAGGGTGATGTGAGCCCGATCGTGCAGCGGTGCCTGGCGTTGATCGAGCAACAGGCCATATGAACACACCATGCCCACAGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCg                                                                                                                                                  <  2:1129680/132‑1 (MQ=255)
                       aGGGTGATGTGAGCCCGATCGTGCAGCGGTGCCTGGCGTTGATCGAGCAACAGGCCATATGAACACACCATGCCCACAGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGa                                                                                                                             <  2:1114095/150‑1 (MQ=255)
                          gTGATGTGAGCCCGATCGTGCAGCGGTGCCTGGCGTTGATCGAGCAACAGGCCATATGAACACACCATGCCCACAGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCt                                                                                                                          <  1:898795/150‑1 (MQ=255)
                             aTGTGAGCCCGATCGTGCAGCGGTGCCTGGCGTTGATCGAGCAACAGGCCATATGAACACACCATGCACACAGGGGCTGTATAGCCCTGCCCTATTGGCTATTCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGtt                                                                                                                       <  2:250105/150‑1 (MQ=255)
                                                         ggCTTTGATCGAGCAACAGGCCATATGAACACACCATGCCCACAGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAggg                                                                                           <  2:1288918/150‑1 (MQ=255)
                                                            gTTGATCGAGCAACAGGCCATATGAACACACCATGCCCACAGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAGGGCTc                                                                                        >  2:1142641/1‑150 (MQ=255)
                                                              tGATCGAGCAACATGCCATATGAACACCCCATGCCCACCGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAGGGCTCaa                                                                                      <  1:645486/150‑1 (MQ=255)
                                                              tGATCGAGCAACAGGCCATATGAACACACCATGCCCACAGGGGCTTTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAGGGCTCaa                                                                                      <  2:468081/150‑1 (MQ=255)
                                                                aTCGAGCAACAGGCCATATGAACACACCATGCCCACAGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAg                                                                                             >  1:427363/1‑141 (MQ=255)
                                                                aTCGAGCAACAGGCCATATGAACACACCATGCCCACAGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAg                                                                                             <  2:427363/141‑1 (MQ=255)
                                                                  cGAGCAACAGGCCATATGAACACACCATGCCCACAGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAGGGCTCAAGACa                                                                                  <  2:1108911/150‑1 (MQ=255)
                                                                    aGCAACAGGCCAGATGAACACACCATGCCCACAGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAGGGCTCAAGACAGt                                                                                >  1:344844/1‑150 (MQ=255)
                                                                        aCAGGCCATATGAACACACCATGCCCACAGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAGGGCTCAAGACAGTCAAt                                                                            >  2:408210/1‑150 (MQ=255)
                                                                          aGGCCATATGAACACACCATGCCCACAGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCa                                                                                                                    <  2:854159/108‑1 (MQ=255)
                                                                          aGGCCATATGAACACACCATGCCCACAGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCa                                                                                                                    >  1:854159/1‑108 (MQ=255)
                                                                          aGGCCATATGAACACACCATGCCCACAGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAGGGCTCAAGACAGTCAATCt                                                                          >  1:460005/1‑150 (MQ=255)
                                                                              cATATGAACACACCATGCCCACAGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAGGGCTCAAGACAGTCAATCTaaaa                                                                      >  1:990901/1‑150 (MQ=255)
                                                                               atatGAACACACCATGCCCACAGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTc                                                                                                                                    <  1:1283015/87‑1 (MQ=255)
                                                                               atatGAACACACCATGCCCACAGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTc                                                                                                                                    >  2:1283015/1‑87 (MQ=255)
                                                                                     acacacCATGCCCACAGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTc                                                                                                                     <  1:1352190/96‑1 (MQ=255)
                                                                                     acacacCATGCCCACAGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTc                                                                                                                     >  2:1352190/1‑96 (MQ=255)
                                                                                      cacacCATGCCCACAGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAGGGCTCAAGACAGTCAATCTAAAATCATCGGc                                                              >  2:1313068/1‑150 (MQ=255)
                                                                                          ccATGCCCACAGGGGCTGTATAGCCCTGCCCCATTGGCGAGGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAGGGCTCAAGACAGTCAATCTAAAATCATCGGCAAAt                                                          <  2:824464/150‑1 (MQ=255)
                                                                                           cATGCCCACAGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAGGGCTCAAGACAGTCAATCTAAAATCATCGGCAAATg                                                         >  1:347589/1‑150 (MQ=255)
                                                                                                ccACAGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAGGGCTCAAGa                                                                                    <  2:282235/118‑1 (MQ=255)
                                                                                                ccACAGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAGGGCTCAAGa                                                                                    >  1:282235/1‑118 (MQ=255)
                                                                                                ccACAGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAGGGCTCAAGACAGTCAATCTAAAATCATCGGCAAATGATCAc                                                    >  2:109258/1‑150 (MQ=255)
                                                                                                  acaGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCa                                                                                              <  2:79767/106‑1 (MQ=255)
                                                                                                  acaGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCa                                                                                              >  1:79767/1‑106 (MQ=255)
                                                                                                  acaGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGATGCGACACGCGAATCGCCAGGGCTCAAGACAGTCAATCTAAAATCATCGGCAAATGATCACcg                                                  >  1:476468/1‑150 (MQ=255)
                                                                                                       ggCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAGGGCTCAAGACAGTCAATCTAAAATCATCGGCAAATGATCACcgc                                                 >  2:1163881/1‑146 (MQ=255)
                                                                                                       ggCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGACCTGTTTCATGCTGCGACACGCGAATCGCCAGGGCTCAAGACAGTCAATCTAAAATCATCGGCAAATGATCACcgc                                                 <  1:1163881/146‑1 (MQ=255)
                                                                                                        gCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAGGGCTCAAGACAGTCAATCTAAAATCATCGGCAAATGATCACCGCGTAAc                                            <  1:1313068/150‑1 (MQ=255)
                                                                                                                    tgccctATTGGCTATGCCGGCCCCGTCTACCCGTCGGTATCGGGCTGTTCCCATTGAGCTGTTTCATGCTGCCACCCGCGAATCGCCAGGGCTCCAGACAGTCCATCTCCAATCATCGGCAAATGATCCCCGCGTAACCAGTCCATaacc                                >  1:378548/1‑148 (MQ=255)
                                                                                                                         tATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAGGGCTCAAGACa                                                                                  <  1:750579/95‑1 (MQ=255)
                                                                                                                         tATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAGGGCTCAAGACa                                                                                  >  2:750579/1‑95 (MQ=255)
                                                                                                                              gCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAGGGCt                                                                                         <  2:753198/83‑1 (MQ=255)
                                                                                                                              gCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAGGGCt                                                                                         >  1:753198/1‑83 (MQ=255)
                                                                                                                                  tGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAGGGCTCAAGACAGTCAATCTAAAATCATCGGCAAATGATCACCGCGTAACCAGTCGATAAACCGTGCaaa                        <  2:638739/144‑1 (MQ=255)
                                                                                                                                  tGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAGGGCTCAAGACAGTCAATCTAAAATCATCGGCAAATGATCACCGCGTAACCAGTCGATAAACCGTGCaaa                        >  1:638739/1‑144 (MQ=255)
                                                                                                                                     cGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAGGGCTCAAGACAGTCAATCTAAAATCATCGGCAAATGATCACCGCGTAACCAGTCGATAAACCGTGCAAACAACTCCGa               <  1:408210/150‑1 (MQ=255)
                                                                                                                                      ggCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAGGGCTCAAGACAGTCAATCTAAAATCATCGGCAAATGATCACCGCGTAACCAGTCGa                                     <  2:883571/127‑1 (MQ=255)
                                                                                                                                      ggCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAGGGATCAAGACAGTCAATCTAAAATCATCGGCAAATGATCACCGCGTAACCAGTCGa                                     >  1:883571/1‑127 (MQ=255)
                                                                                                                                          ccGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAGGGCTCAAGACAGTCAATCTaaaa                                                                      >  1:869018/1‑90 (MQ=255)
                                                                                                                                          ccGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAGGGCTCAAGACAGTCAATCTaaaa                                                                      <  2:869018/90‑1 (MQ=255)
                                                                                                                                           cctataCCGATGGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAGGGCTCAAGACAGTCAATCTAAAATCATCGGCAAATGATCACCGCGTAACCAGTCGATAAACCGTGCAAACAACTCCGATTGCGa         >  1:665458/3‑150 (MQ=255)
                                                                                                                                                  ccATCGGTATAGGGATGCTTCCACTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAGGGCTCACGAAAGTTAATATAAAATCATCGGCAAATGATCACTGCGTAACCAGTCGATAAACCGTGCAAACAACTCCGATTGCGAGTTGAtc  <  1:1142641/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCCGATCATCCTCAGCCGGCGCAAGGGTGATGTGAGCCCGATCGTGCAGCGGTGCCTGGCGTTGATCGAGCAACAGGCCATATGAACACACCATGCCCACAGGGGCTGTATAGCCCTGCCCTATTGGCTATGCCGGCCCCGTATACCCATCGGTATAGGGATGTTCCCATTGAGCTGTTTCATGCTGCGACACGCGAATCGCCAGGGCTCAAGACAGTCAATCTAAAATCATCGGCAAATGATCACCGCGTAACCAGTCGATAAACCGTGCAAACAACTCCGATTGCGAGTTGATC  >  NC_002947/4239217‑4239512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑