Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 39,491 T→G 8.2% Y74D (TAC→GAC)  PP_0036 → transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294739,4910TG 8.2% 132.4 / 4.9 49Y74D (TAC→GAC) PP_0036transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (21/24);  new base G (2/2);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.93e-01

CAATTGATGATCAGCTGGCCGTAGAGTTTTTCGCAACAGCACGTTGTGCCAGTTTCAAACAGGCTGCCCGAGGGCTCAATGTGCCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGCCAGTGCTGAACGAC  >  NC_002947/39342‑39640
                                                                                                                                                     |                                                                                                                                                     
cAATTGATGATCAGCTGGCCGTAGAGTTTTTCGCAACAGCACGTTGTGCCAGTTTCAAACAGGCTGCCCGAGGGCTCAATGTGCCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCt                                                                                                                                                       >  2:907951/1‑150 (MQ=255)
 aaTTGATGATCAGCTGGCCGTAGAGTTTTTCGCAACAGCACGTTGTGCCAGTTTCAAACAGGCTGCCCGAGGGCTCAATGTGCCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTTCTCCCGTGTTTGTCTa                                                                                                                                                      <  2:1294889/150‑1 (MQ=255)
      atgatCAGCTGGCCGTAGAGTTTTTCGCAACAGCACGTTGTGCCAGTTTCAAACAGGCTGCCCGAGGGCTCAATGTGCCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAac                                                                                                                                                 >  2:1412249/1‑150 (MQ=255)
              cTGGCCGTAGAGTTTTTCGCAACAGCACGTTGTGCCAGTTTCAAACAGGCTGCCCGAGGGCTCAATGTGCCAGTGCGAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAg                                                                                                                                         <  1:95581/150‑1 (MQ=255)
                    gTAGAGTTTTTCGCAACAGCACGTTGTGCCAGTTTCAAACAGGCTGCCCGAGGGCTCAATGTGCCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGcc                                                                                                                                   >  2:28923/1‑150 (MQ=255)
                     tAGAGTTTTTCGCAACAGCACGTTGTGCCAGTTTCAAACAGGCTGCCCGAGGGCTCAATGTGCCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGccc                                                                                                                                  >  2:336458/1‑150 (MQ=255)
                     tAGAGTTTTTCGCAACAGCACGTTGTGCCAGTTTCAAACAGGCTGCCCGAGGGCTCAATGTGCCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGccc                                                                                                                                  >  1:133964/1‑150 (MQ=255)
                          tttttCGCAACAGCACGTTGTGCCAGTTTCAAACAGGCTGCCCGAGGGCTCAATGTGCCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGAcc                                                                                                                             >  1:1414909/1‑150 (MQ=255)
                          tttttCGCAACAGCACGTTGTGCCAGTTTCAAACAGGCTGCCCGAGGGCTCAATGTGCCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTAGACAAacacgg                                                                                                                                             <  2:757643/134‑3 (MQ=255)
                          tttttCGCAACAGCACGTTGTGCCAGTTTCAAACAGGCTGCCCGAGGGCTCAATGTGCCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTAGACAAacacgg                                                                                                                                             >  1:757643/1‑132 (MQ=255)
                           ttttCGCAACAGCACGTTGTGCCAGTTTCAAACAGGCTGCCCGAGGGCTCAATGTGCCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGAccc                                                                                                                            <  2:1414909/150‑1 (MQ=255)
                              tCGCAACAGCACGTTGTGCCAGTTTCAAACAGGCTGCCCGAGGGCTCAATGTGCCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACaaa                                                                                                                                                  >  2:92120/1‑125 (MQ=255)
                              tCGCAACAGCACGTTGTGCCAGTTTCAAACAGGCTGCCCGAGGGCTCAATGTGCCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACaaa                                                                                                                                                  <  1:92120/125‑1 (MQ=255)
                                         cGTTGTGCCAGTTTCAAACAGGCTGCCCGAGGGCTCAATGTGCCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAgg                                                                                                              <  1:985170/150‑1 (MQ=255)
                                         cGTTGTGCCAGTTTCAAACAGGCTGCCCGAGGGCTCAATGTGCCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAgg                                                                                                              <  1:907951/150‑1 (MQ=255)
                                              tgCCAGTTTCAAACAGGCTGCCCGAGGGCTCAATGTGCCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAg                                                                                                         <  2:16893/150‑1 (MQ=255)
                                                ccAGTTTCAAACAGGCTGCCCGAGGGCTCAATGTGCCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGcc                                                                                                       >  2:480986/1‑150 (MQ=255)
                                                 cAGTTTCAAACAGGCTGCCCGAGGGCTCAATGTGCCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGGGAGGATCAGCCa                                                                                                      >  1:127123/1‑150 (MQ=255)
                                                  aGTTTCAAACAGGCTGCCCGAGGGCTCAATGTGCCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAg                                                                                                         <  1:585531/146‑1 (MQ=255)
                                                  aGTTTCAAACAGGCTGCCCGAGGGCTCAATGTGCCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAg                                                                                                         >  2:585531/1‑146 (MQ=255)
                                                    tttCAAACAGGCTGCCCGAGGGCTCAATGTGCCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTa                                                                                                                                                      <  2:1397219/99‑1 (MQ=255)
                                                    tttCAAACAGGCTGCCCGAGGGCTCAATGTGCCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTa                                                                                                                                                      >  1:1397219/1‑99 (MQ=255)
                                                     ttCAAACAGGCTGCCCGAGGGCTCAATGTGCCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTAc                                                                                                  <  1:28923/150‑1 (MQ=255)
                                                        aaaCAGGCTGCCCGAGGGCTCAATGTGCCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGc                                                                                                                    >  1:1060139/1‑129 (MQ=255)
                                                        aaaCAGGCTGCCCGAGGGCTCAATGTGCCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGc                                                                                                                    <  2:1060139/129‑1 (MQ=255)
                                                         aaCAGGCTGCCCGAGGGCTCAATGTGCCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCtgt                                                                                              <  2:1198657/150‑1 (MQ=255)
                                                         aaCAGGCTGCCCGAGGGCTCAATGTGCCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCtgt                                                                                              <  2:366469/150‑1 (MQ=255)
                                                              gCTGCCCGAGGGCTCAATGTGCCAGTGGTAGGCCTGCGGAAACGCTTGGAGACACTGGAAGAGCACAGTGGTGCTCCCGTGTTTGGCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAg                                                                                         >  1:1088026/1‑150 (MQ=255)
                                                                   ccGAGGGCTCAATGTGCCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCtt                                                                                    >  1:1136501/1‑150 (MQ=255)
                                                                           tCAATGTGCCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAg                                                                            >  1:135949/1‑150 (MQ=255)
                                                                             aaTGTGCCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGAc                                                                          <  2:135949/150‑1 (MQ=255)
                                                                                   ccAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCtt                                                                    <  1:1317516/150‑1 (MQ=255)
                                                                                   ccAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCtt                                                                    <  2:1136501/150‑1 (MQ=255)
                                                                                   ccAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCtt                                                                    <  1:480986/150‑1 (MQ=255)
                                                                                    cAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTg                                                                   <  1:336458/150‑1 (MQ=255)
                                                                                          tAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGt                                                             >  2:1087120/1‑150 (MQ=255)
                                                                                                   ggAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGctct                                                                                     >  2:40262/1‑117 (MQ=255)
                                                                                                   ggAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGctct                                                                                     <  1:40262/117‑1 (MQ=255)
                                                                                                   ggAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGaaa                                                    >  1:300954/1‑150 (MQ=255)
                                                                                                       ccGCTTGGAGAAACCGGAAGAGCACATTGGTGCTCCCGTGTTTGTTTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTAGGCCCCGACGGCCTTGGCAAGTCCGGTGAAAAGGa                                                <  2:313840/149‑1 (MQ=255)
                                                                                                               agaAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCa                                        <  1:1252916/150‑1 (MQ=255)
                                                                                                                   aCTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCt                                    >  1:916550/1‑150 (MQ=255)
                                                                                                                          gagCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATc                             <  2:133964/150‑1 (MQ=255)
                                                                                                                              acaTTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGa                         >  1:517363/1‑150 (MQ=255)
                                                                                                                                          ccGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGCCAg             >  2:166727/1‑150 (MQ=255)
                                                                                                                                            gtgtTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGCCAGTg           <  1:1412249/150‑1 (MQ=255)
                                                                                                                                            gtgtTTGTAGACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTAc                                                                                                  <  1:1285416/63‑1 (MQ=255)
                                                                                                                                            gtgtTTGTAGACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTAc                                                                                                  >  2:1285416/1‑63 (MQ=39)
                                                                                                                                             tgtTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAGGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGCCAGTg           >  1:1358065/1‑149 (MQ=255)
                                                                                                                                             tgtTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGCGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGCCAGTg           <  2:1358065/149‑1 (MQ=255)
                                                                                                                                                     tACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCCGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCACAGCATTACAATGGCGTGTAGAGAGCCAGTGCTGCTCGAc  >  2:307376/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAATTGATGATCAGCTGGCCGTAGAGTTTTTCGCAACAGCACGTTGTGCCAGTTTCAAACAGGCTGCCCGAGGGCTCAATGTGCCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGCCAGTGCTGAACGAC  >  NC_002947/39342‑39640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑