Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,248,559 C→T 8.5% V504V (GTG→GTA)  PP_3724 ← acyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,248,5590CT 8.5% 220.7 / 10.1 82V504V (GTG→GTA) PP_3724acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (39/36);  new base T (4/3);  total (43/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.69e-01

AGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTG  >  NC_002947/4248415‑4248708
                                                                                                                                                 |                                                                                                                                                     
aGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGcc                                                                                                                                                  >  2:276258/1‑150 (MQ=255)
aGCCTCCAGGGCGCGGGCATCGCGCAGGCTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGcc                                                                                                                                                  >  2:416844/1‑150 (MQ=255)
  cTTCCAGGGCGAGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCg                                                                                                                                                >  2:235989/1‑150 (MQ=255)
    tCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCa                                                                                                                                              <  2:111845/150‑1 (MQ=255)
     ccAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAg                                                                                                                                             <  2:1344996/150‑1 (MQ=255)
      cAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAgg                                                                                                                                            <  1:276258/150‑1 (MQ=255)
       aGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGc                                                                                                                                           >  1:1032475/1‑150 (MQ=255)
       aGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGc                                                                                                                                           >  2:1242902/1‑150 (MQ=255)
       aGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGc                                                                                                                                           <  2:532819/150‑1 (MQ=255)
                 cATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGAc                                                                                                                                 <  1:416844/150‑1 (MQ=255)
                     gcgcAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGCATCAACCG‑CGGGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCTTCACGCCCGCAAACACCGGCAGACCGaa                                                                                                                             >  1:247440/1‑150 (MQ=255)
                      cgcAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAg                                                                                                                            <  2:801315/150‑1 (MQ=255)
                      cgcAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAg                                                                                                                            <  2:3309/150‑1 (MQ=255)
                         aGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAg                                                                                                                            <  1:783047/147‑1 (MQ=255)
                         aGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAg                                                                                                                            >  2:783047/1‑147 (MQ=255)
                           gTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGgcgcg                                                                                                                       >  1:90911/1‑150 (MQ=255)
                            ttCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGTAGACCGAAGgcgcgc                                                                                                                      >  1:414264/1‑150 (MQ=255)
                                ccGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGGCGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGc                                                                                                                                                       <  1:672517/112‑1 (MQ=255)
                                aCGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGc                                                                                                                                                       >  2:672517/1‑113 (MQ=255)
                                 cGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGc                                                                                                                 <  2:601091/150‑1 (MQ=255)
                                          aCCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGa                                                                                                        <  2:90911/150‑1 (MQ=255)
                                           ccAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGAt                                                                                                       <  1:60044/150‑1 (MQ=255)
                                                ttCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGGGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCa                                                                                                  >  1:1314479/1‑150 (MQ=255)
                                                ttCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGTAGGCGCGCAGGGCCGGCTTGGATTGCCa                                                                                                  >  2:434693/1‑150 (MQ=255)
                                                ttCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCa                                                                                                  <  1:719542/150‑1 (MQ=255)
                                                   ggTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTc                                                                                               >  1:142387/1‑150 (MQ=255)
                                                    gTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGc                                                                                                    <  2:411747/144‑1 (MQ=255)
                                                    gTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGc                                                                                                    >  1:411747/1‑144 (MQ=255)
                                                       ttGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTcc                                                                                           >  1:1295780/1‑150 (MQ=255)
                                                       ttGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTcc                                                                                           <  1:1099095/150‑1 (MQ=255)
                                                       ttGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTcc                                                                                           <  1:1099007/150‑1 (MQ=255)
                                                         gtgCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCaa                                                                                         <  2:446985/150‑1 (MQ=255)
                                                              gCAGCTGCGCGCTGACCGCCTTCAGTACAACGGGGT‑CACCGACGCGGGCGGCATATGACACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACAGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTc                                                                                    <  1:945338/150‑1 (MQ=255)
                                                                            aCCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGCGCCTCCTTCCATCGCCATTCGTCCAACACTCCGCTTTCGCCCGTg                                                                      >  2:231894/1‑150 (MQ=255)
                                                                             ccGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGa                                                                                                                                  >  2:699207/1‑89 (MQ=255)
                                                                             ccGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGa                                                                                                                                  <  1:699207/89‑1 (MQ=255)
                                                                              cGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAg                                                                    <  2:1295780/150‑1 (MQ=255)
                                                                              cGCCTTCAGTACCAAGGGGT‑AACCGACGCAGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAg                                                                    >  1:147338/1‑150 (MQ=255)
                                                                               gCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAgg                                                                   >  1:177105/1‑150 (MQ=255)
                                                                                ccTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAggg                                                                  >  1:269064/1‑150 (MQ=255)
                                                                                   tCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCt                                                               >  1:1139833/1‑150 (MQ=255)
                                                                                      gTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCg                                                            <  1:243455/150‑1 (MQ=255)
                                                                                       tACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCAt                                                                                                 >  1:764902/1‑112 (MQ=255)
                                                                                       tACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCAt                                                                                                 <  2:764902/112‑1 (MQ=255)
                                                                                       tACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCaa                                                                                         <  1:1277904/120‑1 (MQ=255)
                                                                                       tACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCaa                                                                                         >  2:1277904/1‑120 (MQ=255)
                                                                                       tACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCgg                                                           <  1:1173360/150‑1 (MQ=255)
                                                                                          cAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGca                                                        >  1:195568/1‑150 (MQ=255)
                                                                                              gggT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGc                                                    <  2:1032475/150‑1 (MQ=255)
                                                                                                     ccGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGAGGGCACAGCGGTAcc                                              >  2:988205/1‑150 (MQ=255)
                                                                                                      cGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTAccc                                             <  2:195568/150‑1 (MQ=255)
                                                                                                       gACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCg                                            >  2:536698/1‑150 (MQ=255)
                                                                                                          gcgggcggCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGcag                                         <  2:385399/150‑2 (MQ=255)
                                                                                                             ggcggcGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGc                                      >  1:332572/1‑150 (MQ=255)
                                                                                                                   gTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGcc                                <  2:1139833/150‑1 (MQ=255)
                                                                                                                   ctCTGCCACGGCGCGTTCGGGCGTCCTCAGCAGGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCCCACAGCCTCGcc                                <  2:147338/149‑1 (MQ=255)
                                                                                                                      tGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTg                             >  2:1393567/1‑150 (MQ=255)
                                                                                                                      tGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTg                             >  2:509570/1‑150 (MQ=255)
                                                                                                                      tGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTg                             <  2:269064/150‑1 (MQ=255)
                                                                                                                        ccACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGt                           <  1:584500/150‑1 (MQ=255)
                                                                                                                         cACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTc                          >  2:715080/1‑150 (MQ=255)
                                                                                                                           cGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCa                        >  1:341831/1‑150 (MQ=255)
                                                                                                                            ggCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCAt                       >  1:1322577/1‑150 (MQ=255)
                                                                                                                             gccagtacGGGCGTGCTCTGCACGCCGGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATc                      >  2:1124285/8‑150 (MQ=255)
                                                                                                                               gcgTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTTAAACAGTCGGCTTGCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCaa                    <  1:1242902/150‑1 (MQ=255)
                                                                                                                                atctCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGAAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAAc                   <  2:970329/147‑1 (MQ=255)
                                                                                                                                 ctgcGGGCGTGCTGAGTACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTgg                            >  1:1075677/4‑140 (MQ=255)
                                                                                                                                 ctgcGGGCGTGCTGAGTACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTgg                            <  2:1075677/137‑1 (MQ=255)
                                                                                                                                  tgcGGGCGTGCTGAGTACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGAt                                                                                                       <  1:150480/62‑1 (MQ=25)
                                                                                                                                  tgcGGGCGTGCTGAGTACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGAt                                                                                                       >  2:150480/3‑64 (MQ=25)
                                                                                                                                     gggCGTGCTGAGTACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTgg                            <  1:65052/136‑1 (MQ=255)
                                                                                                                                     gggCGTGCTGAGTACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTgg                            >  2:65052/1‑136 (MQ=255)
                                                                                                                                     gggCGTGCTGAGTACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTg              >  1:965883/1‑150 (MQ=255)
                                                                                                                                     gggCGTACTCAGCACGCCCGCAGGCGCAGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAAccagat              >  1:970329/1‑146 (MQ=255)
                                                                                                                                     gggCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTg              >  1:242353/1‑150 (MQ=255)
                                                                                                                                      ggCGTACTCAGCACGCCCGCAGGCACCGGCAGAACGCTCTCTCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTgg             <  1:434693/150‑1 (MQ=255)
                                                                                                                                          tACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGt         >  1:315175/1‑150 (MQ=255)
                                                                                                                                            cTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTc       >  2:638098/1‑150 (MQ=255)
                                                                                                                                            cTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTc       >  2:576424/1‑150 (MQ=255)
                                                                                                                                             tCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCt      >  1:654681/1‑150 (MQ=255)
                                                                                                                                             tCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCAATCGGTTCt      >  1:60305/1‑150 (MQ=255)
                                                                                                                                               aGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCttt    <  2:142387/150‑1 (MQ=255)
                                                                                                                                               aGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCttt    <  2:478436/150‑1 (MQ=255)
                                                                                                                                                gCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCtttt   <  2:247440/150‑1 (MQ=255)
                                                                                                                                                 cACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTg  <  2:1314479/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
AGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGT‑AACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTCAGCACGCCCGCAGGCACCGGCAGACCGAAGGCGCGCAGGGCCTGCTTGGATTGCCATTCGTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTG  >  NC_002947/4248415‑4248708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑