Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,284,691 A→G 6.3% I18V (ATT→GTT)  hbd → 3‑hydroxybutyryl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,284,6910AG 6.3% 243.7 / 4.1 79I18V (ATT→GTT) hbd3‑hydroxybutyryl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (35/39);  new base G (2/3);  total (37/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

ATCCAGGGCCGCTACGCCCTGGCCACGATGTGTATCGGCGGTGGCCAAGGCATCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCGCAGCCGC  >  NC_002947/4284549‑4284839
                                                                                                                                              |                                                                                                                                                    
aTCCAGGGCCGCTACGCCCTGGCCACGATGTGTATCGGCGGTGGCCAAGGCATCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCa                                                                                                                                               >  2:892718/1‑150 (MQ=255)
 tCCAGGGCCGCTACGCCCTGGCCACGATGTGTATCGGCGGTGGCCAAGGCATCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAATGCCGTTGCCCat                                                                                                                                              <  1:254649/150‑2 (MQ=255)
 tCCAGGGCCGCTACGCCCTGGCCACGATGTGTATCGGCGGTGGCCAAGGCATCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAg                                                                                                                                              >  2:978079/1‑150 (MQ=255)
  ccAGGGCCGCTACGCCCTGGCCACGATGTGTATCGGCGGTGGCCAAGGCATCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAATGCCGTTGCCCatg                                                                                                                                             <  2:935476/150‑3 (MQ=255)
  ccAGGGCCGCTACGCCCTGGCCACGATGTGTATCGGCGGTGGCCAAGGCATCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAgg                                                                                                                                             <  2:1179362/150‑1 (MQ=255)
  ccAGGGCCGCTACGCCCTGGCCACGATGTGTATCGGCGGTGGCCAAGGCATCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAgg                                                                                                                                             <  2:1048060/150‑1 (MQ=255)
     gggCCGCTACGCCCTGGCCACGATGTGTATCGGCGGTGGCCAAGGCATCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGGTGACCCATGAACAGGTTAAAGAACTCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGgtgt                                                                                                                                          >  1:1236904/1‑150 (MQ=255)
      ggCCGCTACGCCCTGGCCACGATGTGTATCGGCGGTGGCCAAGGCATCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGtgtg                                                                                                                                         >  1:840157/1‑150 (MQ=255)
         cGCTACGCCCTGGCCACGATGTGTATCGGCGGTGGCCAAGGCATCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTgcgc                                                                                                                                      <  2:511551/150‑1 (MQ=255)
         cGCTACGCCCTGGCCACGATGTGTATCGGCGGTGGCCAAGGCATCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTgcgc                                                                                                                                      <  1:95393/150‑1 (MQ=255)
                cccTGGCCACGATGTGTATCGGCGGTGGCCAAGGCATCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGcc                                                                                                                               >  1:789374/1‑150 (MQ=255)
                 ccTGGCCACGATGTGTATCGGCGGTGGCCAAGGCATCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAATGCCGTTGCCCa                                                                                                                                               <  1:989463/133‑1 (MQ=255)
                 ccTGGCCACGATGTGTATCGGCGGTGGCCAAGGCATCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAATGCCGTTGCCCa                                                                                                                                               >  2:989463/1‑133 (MQ=255)
                 ccTGGCCACGATGTGTATCGGCGGTGGCCAAGGCATCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCg                                                                                                                              <  2:487261/150‑1 (MQ=255)
                 ccTGGCCACGATGTGTATCGGCGGTGGCCAAGGCATCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCg                                                                                                                              >  2:854515/1‑150 (MQ=255)
                 ccTGGCCACGATGTGTATCGGCGGTGGCCAAGGCATCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCg                                                                                                                              <  2:42559/150‑1 (MQ=255)
                         cGATGTGTATCGGCGGTGGCCAAGGCATCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAg                                                                                                                      <  1:1305308/150‑1 (MQ=255)
                           aTGTGTATCGGCGGTGGCCAAGGCATCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGt                                                                                                                    >  2:21719/1‑150 (MQ=255)
                                  tCGGCGGTGGCCAAGGCATCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGctgctg                                                                                                             <  1:892718/150‑1 (MQ=255)
                                    ggcggTGGCCAAGGCATCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCtggt                                                                                                           <  2:789374/150‑1 (MQ=255)
                                    ggcggTGGCCAAGGCATCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCtggt                                                                                                           <  1:854515/150‑1 (MQ=255)
                                     gcggTGGCCAAGGCATCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCtggtg                                                                                                          <  1:950725/150‑1 (MQ=255)
                                     gcggTGGCCAAGGCATCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCtggtg                                                                                                          >  1:627586/1‑150 (MQ=255)
                                     gcggTGGCCAAGGCATCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCtggtg                                                                                                          <  1:978079/150‑1 (MQ=255)
                                             cAAGGCATCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTc                                                                                                  >  1:445795/1‑150 (MQ=255)
                                             cAAGGCATCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGACCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTc                                                                                                  >  1:804083/1‑150 (MQ=255)
                                              aaGGCATCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTcc                                                                                                 <  2:1236904/150‑1 (MQ=255)
                                                   aTCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGt                                                                                                     >  1:740864/1‑141 (MQ=255)
                                                   aTCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGt                                                                                                     <  2:740864/141‑1 (MQ=255)
                                                    tCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCg                                                                                                                              <  2:653456/115‑1 (MQ=255)
                                                    tCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCg                                                                                                                              >  1:653456/1‑115 (MQ=255)
                                                      gCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAgc                                                                                         <  2:242192/150‑1 (MQ=255)
                                                      gCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAgc                                                                                         >  2:8803/1‑150 (MQ=255)
                                                              gtTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAgc                                                                                 >  2:175079/1‑150 (MQ=255)
                                                                 cGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGcgcg                                                                              <  1:175079/150‑1 (MQ=255)
                                                                 cGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGcgcg                                                                              <  1:21719/150‑1 (MQ=255)
                                                                  gAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCgg                                                                             >  2:490716/1‑150 (MQ=255)
                                                                  gAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCgg                                                                             <  1:8803/150‑1 (MQ=255)
                                                                   aGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGgt                                                                            <  2:627586/150‑1 (MQ=255)
                                                                      gcgTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGtgtg                                                                         <  2:1037001/150‑1 (MQ=255)
                                                                      gcgTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGtgtg                                                                         <  2:942632/150‑1 (MQ=255)
                                                                        gTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGc                                                                       >  2:886630/1‑150 (MQ=255)
                                                                            gagggaggCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCAcc                                                                   <  2:212798/150‑1 (MQ=255)
                                                                              gggaggCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGAt                                                                                                       >  2:549078/1‑112 (MQ=255)
                                                                              gggaggCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGAt                                                                                                       <  1:549078/112‑1 (MQ=255)
                                                                              gggaggCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCtt                                                                 <  1:316670/150‑1 (MQ=255)
                                                                                 aggCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAg                                                              >  2:484411/1‑150 (MQ=255)
                                                                                   gCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTAc                                                                                                                         <  1:340572/89‑1 (MQ=255)
                                                                                   gCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTAc                                                                                                                         >  2:340572/1‑89 (MQ=255)
                                                                                    cTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCaa                                                           <  1:1326118/150‑1 (MQ=255)
                                                                                    cTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCaa                                                           <  1:1326234/150‑1 (MQ=255)
                                                                                       acacATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTg                                                                                                                                                   <  2:1080471/59‑1 (MQ=255)
                                                                                       acacATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTg                                                                                                                                                   >  1:1080471/1‑59 (MQ=255)
                                                                                           ctGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTATACGACGCCGTGTTCGATCGTGGGGTTGCCACCTTGAGGAAGTACGTc                                                    >  2:1225074/2‑150 (MQ=255)
                                                                                           aTGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTc                                                    <  1:886630/150‑1 (MQ=255)
                                                                                             gAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGgt                                                                                                                                            <  1:760019/60‑1 (MQ=255)
                                                                                             gAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGgt                                                                                                                                            >  2:760019/1‑60 (MQ=255)
                                                                                              aGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAg                                                 <  2:445795/150‑1 (MQ=255)
                                                                                                    gAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAg                                           >  1:71817/1‑150 (MQ=255)
                                                                                                    gAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAg                                           <  2:840157/150‑1 (MQ=255)
                                                                                                     aaaaGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGa                                                  >  2:949146/1‑142 (MQ=255)
                                                                                                     aaaaGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGa                                                  <  1:949146/142‑1 (MQ=255)
                                                                                                          aTCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCACTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCCCGATCGCGGTGTGGCCACATTGAGCAAGAACCTCGAGCGCCAGGTCAga                                     <  2:794520/150‑2 (MQ=255)
                                                                                                           tCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCa                                    >  1:1389889/1‑150 (MQ=255)
                                                                                                             gCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAg                                  <  2:330411/150‑1 (MQ=255)
                                                                                                              ccGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGAGGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAgg                                 >  1:1240808/1‑150 (MQ=255)
                                                                                                                gTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGc                               <  1:472323/150‑1 (MQ=255)
                                                                                                                    tCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTgg                                                                        <  1:934917/105‑1 (MQ=255)
                                                                                                                    tCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTgg                                                                        >  2:934917/1‑105 (MQ=255)
                                                                                                                        cgcgGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGa                       >  1:1212845/1‑150 (MQ=255)
                                                                                                                           gggCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGa                                                                                   <  1:451330/87‑1 (MQ=255)
                                                                                                                           gggCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGa                                                                                   >  2:451330/1‑87 (MQ=255)
                                                                                                                              cACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGa                 >  2:501013/1‑150 (MQ=255)
                                                                                                                               aCCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGAc                >  1:704678/1‑150 (MQ=255)
                                                                                                                               aCCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCAGGCTACAAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGAc                >  1:274062/1‑150 (MQ=255)
                                                                                                                                  ctGGGCAATGCCGTTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGACAAg             >  2:676326/2‑150 (MQ=255)
                                                                                                                                      gCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCg         >  1:47269/1‑150 (MQ=255)
                                                                                                                                      gCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCg         >  1:134457/1‑150 (MQ=255)
                                                                                                                                             cATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCGCAgccgc  >  1:1363713/1‑150 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
ATCCAGGGCCGCTACGCCCTGGCCACGATGTGTATCGGCGGTGGCCAAGGCATCGCCGTCGTGTTCGAGCGCGTCTGAGGGAGGCTGACACATGAGCATTGAAAAGATCGCCGTGATCGGCGCGGGCACCATGGGCAACGGCATTGCCCAGGTGTGCGCCATTGCCGGCTACCAGGTGCTGCTGGTGGATGTTTCCGACGCAGCGCTCGAGCGCGGTGTGGCCACCTTGAGCAAGAACCTCGAGCGCCAGGTCAGCAAGGGCACTCTCGACGCCGACAAGGCCGCAGCCGC  >  NC_002947/4284549‑4284839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑