| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 381,859 | T→A | 11.8% | D230V (GAC→GTC) | PP_0317 ← | methyl‑accepting chemotaxis transducer |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 381,859 | 0 | T | A | 11.8% | 143.5 / 10.9 | 51 | D230V (GAC→GTC) | PP_0317 | methyl‑accepting chemotaxis transducer |
| Reads supporting (aligned to +/- strand): ref base T (18/27); new base A (2/4); total (20/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01 | |||||||||||
TTCTGCCGCCGAGGCCAGCTGGGTGGCGGAGCCGGCGATCTGCTGCAAGGTATCGCGCAGCTTGTCCTGCATGCGCGCCATGGCGCGCAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGACGATACTGCGGGTCAGCAGGAAGGCACAGGCGAAGGTCAGTACGGTGGCGGCAACCAGCAGGCCGATCACCAGGGCGAAAGCGCCGGCATACTGGTTGGC > NC_002947/381714‑382004 | ttctGCCGCCGAGGCCAGCTGGGTGGCGGAGCCGGCGATCTGCTGCAAGGTATCGCGCAGCTTGTCCTGCATGCGCGCCATGGCGCGCAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGc < 1:174713/150‑1 (MQ=255) tctGCCGCCGAGGCCAGCTGTGTGGCGGAGCCGGCGATCTGCTGCAAGGTATCGCGCAGCTTGTCCTGCATGCGCGCCATGGCGCGCAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCg < 1:782250/150‑1 (MQ=255) tGCCGCCGAGGCCAGCTGGGTGGCGGAGCCGGCGATCTGCTGCAAGGTATCGCGCAGCTTGTCCTGCATGCGCGCCATGGCGCGCAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGAc < 2:1345849/150‑1 (MQ=255) ccgccgAGGCCAGCTGGGTGGCGGAGCCGGCGATCTGCTGCAAGGTATCGCGCAGCTTGTCCTGCATGCGCGCCATGGCGCGCAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGACGGCGAcct > 1:390910/1‑148 (MQ=255) cgccgAGGCCAGCTGGGTGGCGGAGCCGGCGATCTGCTGCAAGGTATCGCGCAGCTTGTCCTGCATGCGCGCCATGGCGCGCAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGACGGCGACCTg < 1:280732/150‑1 (MQ=255) gccgAGGCCAGCTGGGTGGCGGAGCCGGCGATCTGCTGCAAGGTATCGCGCAGCTTGTCCTGCATGCGCGCCATGGCGCGCAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGACGGCGACCTga < 2:750865/150‑2 (MQ=255) gccgAGGCCAGCTGGGTGGCGGAGCCGGCGATCTGCTGCAAGGTATCGCGCAGCTTGTCCTGCATGCGCGCCATGGCGCGCAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGACGGCGACCTga < 2:390910/150‑2 (MQ=255) ccgAGGCCAGCTGGGTGGCGGAGCCGGCGATCTGCTGCAAGGTATCGCGCAGCTTGTCCTGCATGCGCGCCATGGCGCGCAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCt > 1:1166670/1‑150 (MQ=255) gAGGCCAGCTGGGTGGCGGAGCCGGCGATCTGCTGCAAGGTATCGCGCAGCTTGTCCTGCATGCGCGCCATGGCGCGCAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCg < 1:174757/150‑1 (MQ=255) gggTGGCGGAGCCGGCGATCTGCTGCAAGGTATCGCGCAGCTTGTCCTGCATGCGCGCCATGGCGCGCAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGa < 2:999394/150‑1 (MQ=255) ggTGGCGGAGCCGGCGATCTGCTGCAAGGTATCGCGCAGCTTGACCTGCATGCGCGCCATGGCGCGCAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAg < 2:1099388/150‑1 (MQ=255) gTGGCGGAGCCGGCGATCTGCTGCAAGGTATCGCGCAGCTTGTCCTGCATGCGCGCCATGGCGCGCAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGt < 2:212506/150‑1 (MQ=255) ccGGCGATCTGCTGCAAGGTATCGCGCAGCTTGTCCTGCATGCGCGCCATGGCGCGCAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCg > 2:957525/1‑150 (MQ=255) cggctgctAGGTATCGCGCAGCTTGTCCTGCATGCCCTCGATGTCGCGCAACAGCCGGGCGGCTTCGTCCGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCtt < 1:1384535/148‑1 (MQ=255) ctgcAAGGTATCGCGCAGCTTGTCCTGCATGCGCGCCATGGCGCGCAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGAc < 2:1357419/150‑1 (MQ=255) tgcAAGGTCGAGCGCAGCGTGTCCTTCATGCGCGCCATGGCGCGCAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACGTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGACg > 2:1156283/1‑150 (MQ=255) ggTATCGCGCAGCTTGTCCTGCATGCGCGCCATGGCGCGCAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGACGATACt > 2:795868/1‑150 (MQ=255) tCGCGCAGCTTGTCCTGCATGCGCGCCATGGCGCGCAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGACGATACTGCgg < 1:926690/150‑1 (MQ=255) gcgcAGCTTGTCCTGCATGCGCGCCATGGCGCGCAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGACGATACTGc > 2:844744/1‑146 (MQ=255) gcgcAGCTTGTCCTGCATGCGCGCCATGGCGCGCAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGACGATACTGc < 1:844744/146‑1 (MQ=255) gcgcAGCTTGTCCTGCATGCGCGCCATGGCGCGCAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGACGATACTGCGGGt > 2:1069213/1‑150 (MQ=255) gcgcAGCTTGTCCTGCATGCGCGCCATGGCGCGCAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCA‑GTCGCCGACGGCGAcctga > 2:633018/1‑98 (MQ=255) gcgcAGCTTGTCCTGCATGCGCGCCATGGCGCGCAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCA‑GTCGCCGACGGCGACCTga < 1:633018/102‑2 (MQ=255) gCTTGTCCTGCATGCGCGCCATGGCGCGCAACAGCCGAGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGACGATACTGCGGGTcagca < 1:1325277/150‑1 (MQ=255) ttGTCCTGCATGCGCGCCATGGCGCGCAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGACGATACTGCGGGTCAGCAgg > 1:1122008/1‑150 (MQ=255) tCCTGCATGCGCGCCATGGCGCGCAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGACGATa > 2:146015/1‑132 (MQ=255) tCCTGCATGCGCGCCATGGCGCGCAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGACGATa < 1:146015/132‑1 (MQ=255) tCCTGCATGCGCGCCATGGCGCGCAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGACGATACTGCGGGTCAGCAGGAAg < 2:839601/150‑1 (MQ=255) cTGCATGCGCGCCATGGCGCGCAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGACGATACTGCGGGTCAGCAGGAAGGc < 2:1166670/150‑1 (MQ=255) cTGCATGCGCGCCATGGCGCGCAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGACGATACTGCGGGTCAGCAGGAAGGc > 2:1319884/1‑150 (MQ=255) tGCATGCGCGCCATGGCGCGCAACAGCCGGGCGGCTTCGGCGGTGTCCTCTGCGCGGCTGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGACGATACTGCGGGTCGCCAGGACGGcc > 2:487907/1‑149 (MQ=255) cgcCATGGCGCGCAACAGCCGGGCGGCTTCGTTGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGACGATACTGCGGGTCAGCAGGAAGGCACAGGCGaa < 1:1215599/150‑1 (MQ=255) gcCATGGCGCGCAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGACGATACTGCGGGTCAGCAGGAAGGCACAGGCGAAg < 1:1319884/150‑1 (MQ=255) tGGCGCGCAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGACGATACTGCGGGTCAGCAGGAAGGCACAGGCGAAGGTCa < 2:1273966/150‑1 (MQ=255) cgcAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGACGATACTGCGGGTCAGCAGGAAGGCACAGGCGAAGGTCAGTACg < 2:1122008/150‑1 (MQ=255) aaCAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGa < 1:837081/82‑1 (MQ=255) aaCAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGa > 2:837081/1‑82 (MQ=255) gCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGAc > 2:547/1‑100 (MQ=255) gCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGAc < 1:547/100‑1 (MQ=255) gttggTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGACGATACTGCGGGTCAGCAGGaa < 2:356837/105‑1 (MQ=255) gttggTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGACGATACTGCGGGTCAGCAGGaa > 1:356837/4‑108 (MQ=255) gtcgGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGACGATACTGCGGGTCAGCAGGAAGGCACAGGCGAAGGTCAGTACGGTGGCGGCAACCAGCAGGcc < 1:1074078/150‑1 (MQ=255) cccTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGACGATACTGCGGGTCAGCAGGAAGGCACAGGCGAAGGTCAGTACGGTggc > 2:1150591/1‑128 (MQ=255) cccTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGACGATACTGCGGGTCAGCAGGAAGGCACAGGCGAAGGTCAGTACGGTggc < 1:1150591/128‑1 (MQ=255) ccTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGACGATACTGCGGGTCAGCAGGAAGGCACAGGCGAAGGTCAGTACGGTGGCGGCAACCAGCAGGCCGATCACCa > 1:320587/1‑150 (MQ=255) cgGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGACGATACTGCGGGTCAGCAGGAAGGCACAGGCGAAGGTCAGTACGGTGGCGGCAACCAGCAGGCCGATCACCAGGGCGaaa < 1:795868/150‑1 (MQ=255) gTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGACGATACTGCGGGTCAGCAGGAAGGCACAGGCGAAGGTCAGTACGGTGGCGGCAACCAGCAGGCCGATCACCAGGGCGAAAGCGCCGGc > 2:1395052/1‑150 (MQ=255) gTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGACGATACTGCGGGTCAGCAGGAAGGCACAGGCGAAGGTCAGTACGGTGGCGGCAACCAGCAGGCCGATCACCAGGGCGAAAGCGCCGGc > 2:842171/1‑150 (MQ=255) tCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGACGATACTGCGGGTCAGCAGGAAGGCACAGGCGAAGGTCAGTACGGTGGCGGCAACCAGCAGGCCGATCACCAGGGc < 2:121018/133‑1 (MQ=255) tCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGACGATACTGCGGGTCAGCAGGAAGGCACAGGCGAAGGTCAGTACGGTGGCGGCAACCAGCAGGCCGATCACCAGGGc > 1:121018/1‑133 (MQ=255) gCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGACGATACTGCGGGTCAGCAGGAAGGCACAGGCGAAGGTCAGTACGGTGGCGGCAACCAGCAGGCCGATCACCAGGGCGAAAGCGCCGGCATACTGGTTggc < 1:1069213/150‑1 (MQ=255) | TTCTGCCGCCGAGGCCAGCTGGGTGGCGGAGCCGGCGATCTGCTGCAAGGTATCGCGCAGCTTGTCCTGCATGCGCGCCATGGCGCGCAACAGCCGGGCGGCTTCGTCGGTGCCCTCTGCGCGGATGACGTGGGTCAGGTCGCCGTCGGCGACTTGCTCGGCGCATTTGAGTGCTTCTTCGATCGGCTTGACGATACTGCGGGTCAGCAGGAAGGCACAGGCGAAGGTCAGTACGGTGGCGGCAACCAGCAGGCCGATCACCAGGGCGAAAGCGCCGGCATACTGGTTGGC > NC_002947/381714‑382004 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |