Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,301,102 A→G 7.4% T75A (ACG→GCG)  PP_3774 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,301,1020AG 7.4% 194.3 / 4.1 67T75A (ACG→GCG) PP_3774hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (31/31);  new base G (2/3);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

AGCTGAACGGTGCTGAAGCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCAACATCACC  >  NC_002947/4300953‑4301251
                                                                                                                                                     |                                                                                                                                                     
aGCTGAACGGTGCTGAAGCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCACAGGCGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGa                                                                                                                                                       <  1:728203/150‑1 (MQ=255)
     aaCGGTGCTGAAGCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTg                                                                                                                                                  <  2:229354/150‑1 (MQ=255)
              gAAGCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGcc                                                                                                                                         >  1:614576/1‑150 (MQ=255)
               aaGCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGATGCTGGCCCGCGccc                                                                                                                                        >  1:350820/1‑150 (MQ=255)
                aGCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGGCGCTGGCCAGCGTCCa                                                                                                                                       <  2:479866/150‑1 (MQ=255)
                 gCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAg                                                                                                                                      <  1:102097/150‑1 (MQ=255)
                       gCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCAc                                                                                                                                <  2:116332/150‑1 (MQ=255)
                        cccGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACt                                                                                                                               <  1:636619/150‑1 (MQ=255)
                         ccGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTg                                                                                                                              >  1:1109240/1‑150 (MQ=255)
                                 ccAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGcc                                                                                                                      >  2:481885/1‑150 (MQ=255)
                                  cAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCa                                                                                                                     >  2:1357984/1‑150 (MQ=255)
                                   aGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAg                                                                                                                    >  1:665321/1‑150 (MQ=255)
                                      aTCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACa                                                                                                                 >  1:533928/1‑150 (MQ=255)
                                        cGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAAt                                                                                                               <  1:1272137/150‑1 (MQ=255)
                                               gTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCGAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTg                                                                                                        >  2:1198002/1‑150 (MQ=255)
                                                   tCAGCGATGGCTCGATCCAGTTCTCAGCCCCAAACAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCa                                                                                                    >  1:629941/1‑150 (MQ=255)
                                                     aGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGAGGCTTCGAGGAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAg                                                                                                  >  1:248905/1‑150 (MQ=255)
                                                       cGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGTAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGt                                                                                                <  2:422143/150‑1 (MQ=255)
                                                           ggCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGGCGCTGGCCAGCGTCCAgt                                                                                                                                     <  1:233509/109‑2 (MQ=255)
                                                           ggCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGGCGCTGGCCAGCGTCCAgt                                                                                                                                     >  2:233509/1‑108 (MQ=255)
                                                             cTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCa                                                                                                                     >  1:953118/1‑123 (MQ=255)
                                                             cTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCa                                                                                                                     <  2:953118/123‑1 (MQ=255)
                                                               cGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCgg                                                                                        <  1:1090318/150‑1 (MQ=255)
                                                                gATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGgc                                                                                       >  1:940257/1‑150 (MQ=255)
                                                                  tCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTg                                                                                                                              >  2:1229318/1‑109 (MQ=255)
                                                                  tCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTg                                                                                                                              <  1:1229318/109‑1 (MQ=255)
                                                                   ccAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCa                                                                                    >  1:1147890/1‑150 (MQ=255)
                                                                    cAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAg                                                                                   >  2:1007410/1‑150 (MQ=255)
                                                                          tcAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCCGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAggtg                                                                             <  1:1357984/150‑1 (MQ=255)
                                                                          tcAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAggtg                                                                             >  1:1406615/1‑150 (MQ=255)
                                                                           cAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAggtgg                                                                            <  2:629941/150‑1 (MQ=255)
                                                                           cAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAggtgg                                                                            <  2:1406615/150‑1 (MQ=255)
                                                                                 caaccaaAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGc                                                                                                                                                    <  1:30805/72‑1 (MQ=255)
                                                                                 caaccaaAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGc                                                                                                                                                    >  2:30805/1‑72 (MQ=255)
                                                                                     caaAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCa                                                                  >  1:538204/1‑150 (MQ=255)
                                                                                        aGGGGCTTCGAGCAAAAGGACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGAGGCTGGCCAGCGCCCAGGATCCCTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGAt                                                               <  2:912904/150‑1 (MQ=255)
                                                                                            gCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCAc                                                           <  2:1147890/150‑1 (MQ=255)
                                                                                             cTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACg                                                          >  1:751520/1‑150 (MQ=255)
                                                                                              ttCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGc                                                                                                                       >  1:737390/1‑88 (MQ=255)
                                                                                              ttCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGc                                                                                                                       <  2:737390/88‑1 (MQ=255)
                                                                                                 gAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTAcg                                                      >  1:1405550/1‑150 (MQ=255)
                                                                                                   gCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATc                                                                                                                         <  2:1006758/81‑1 (MQ=255)
                                                                                                   gCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATc                                                                                                                         >  1:1006758/1‑81 (MQ=255)
                                                                                                   gCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTAcgcg                                                    <  2:1315024/150‑1 (MQ=255)
                                                                                                    cAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCgg                                                   <  1:1274608/150‑1 (MQ=255)
                                                                                                       aaGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGga                                                >  1:493779/1‑150 (MQ=255)
                                                                                                          cACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACt                                                                                                                               <  1:202228/68‑1 (MQ=255)
                                                                                                          cACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACt                                                                                                                               >  2:202228/1‑68 (MQ=255)
                                                                                                             ccACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACgg                                          >  2:685365/1‑150 (MQ=255)
                                                                                                               acaGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGcc                                        >  2:298728/1‑150 (MQ=255)
                                                                                                                        gCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTaa                               >  2:11255/1‑150 (MQ=255)
                                                                                                                                tGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTc                       <  1:1007410/150‑1 (MQ=255)
                                                                                                                                 ggACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCt                      <  1:1198002/150‑1 (MQ=255)
                                                                                                                                  gACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTg                     <  2:533928/150‑1 (MQ=255)
                                                                                                                                   aCGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTgg                    <  2:538204/150‑1 (MQ=255)
                                                                                                                                      gAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAgg                                                                                                 <  2:1083745/70‑1 (MQ=255)
                                                                                                                                      gAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAgg                                                                                                 >  1:1083745/1‑70 (MQ=255)
                                                                                                                                         aCGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAg              <  2:665321/150‑1 (MQ=255)
                                                                                                                                          cGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAgg             <  2:614576/150‑1 (MQ=255)
                                                                                                                                             aaGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCa          >  2:1283873/1‑150 (MQ=255)
                                                                                                                                             aaGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCa          >  2:1073190/1‑150 (MQ=255)
                                                                                                                                             aaGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCa          <  1:685365/150‑1 (MQ=255)
                                                                                                                                               tccTGGGCGCTGGCCAGCGTCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAg                             >  1:1347213/3‑129 (MQ=255)
                                                                                                                                               tccTGGGCGCTGGCCAGCGTCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAg                             <  2:1347213/127‑1 (MQ=255)
                                                                                                                                               gACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCAAc        <  1:1283873/150‑1 (MQ=255)
                                                                                                                                               aaCTGGACGCTGGCCAGCGTCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCTGTAAAGGTGTTCTGGAACAAGGGCAAc        <  2:458527/149‑1 (MQ=255)
                                                                                                                                                  tGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAg                                                                 >  2:540710/1‑90 (MQ=255)
                                                                                                                                                  tGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAg                                                                 <  1:540710/90‑1 (MQ=255)
                                                                                                                                                     aCGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCAACATCAcc  <  2:751520/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGCTGAACGGTGCTGAAGCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCAACATCACC  >  NC_002947/4300953‑4301251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑