Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,301,115 C→T 8.8% A79V (GCC→GTC)  PP_3774 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,301,1150CT 8.8% 173.9 / 5.5 65A79V (GCC→GTC) PP_3774hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (29/30);  new base T (2/4);  total (31/34)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

GAAGCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCAACATCACCCTGGGGTTTCGGC  >  NC_002947/4300967‑4301264
                                                                                                                                                    |                                                                                                                                                     
gAAGCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGcc                                                                                                                                                      >  1:614576/1‑150 (MQ=255)
 aaGCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGATGCTGGCCCGCGccc                                                                                                                                                     >  1:350820/1‑150 (MQ=255)
  aGCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGGCGCTGGCCAGCGTCCa                                                                                                                                                    <  2:479866/150‑1 (MQ=255)
   gCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAg                                                                                                                                                   <  1:102097/150‑1 (MQ=255)
         gCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCAc                                                                                                                                             <  2:116332/150‑1 (MQ=255)
          cccGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACt                                                                                                                                            <  1:636619/150‑1 (MQ=255)
           ccGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTg                                                                                                                                           >  1:1109240/1‑150 (MQ=255)
                   ccAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGcc                                                                                                                                   >  2:481885/1‑150 (MQ=255)
                    cAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCa                                                                                                                                  >  2:1357984/1‑150 (MQ=255)
                     aGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAg                                                                                                                                 >  1:665321/1‑150 (MQ=255)
                        aTCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACa                                                                                                                              >  1:533928/1‑150 (MQ=255)
                          cGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAAt                                                                                                                            <  1:1272137/150‑1 (MQ=255)
                                 gTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCGAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTg                                                                                                                     >  2:1198002/1‑150 (MQ=255)
                                     tCAGCGATGGCTCGATCCAGTTCTCAGCCCCAAACAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCa                                                                                                                 >  1:629941/1‑150 (MQ=255)
                                       aGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGAGGCTTCGAGGAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAg                                                                                                               >  1:248905/1‑150 (MQ=255)
                                         cGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGTAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGt                                                                                                             <  2:422143/150‑1 (MQ=255)
                                             ggCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGGCGCTGGCCAGCGTCCAgt                                                                                                                                                  <  1:233509/109‑2 (MQ=255)
                                             ggCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGGCGCTGGCCAGCGTCCAgt                                                                                                                                                  >  2:233509/1‑108 (MQ=255)
                                               cTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCa                                                                                                                                  >  1:953118/1‑123 (MQ=255)
                                               cTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCa                                                                                                                                  <  2:953118/123‑1 (MQ=255)
                                                 cGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCgg                                                                                                     <  1:1090318/150‑1 (MQ=255)
                                                  gATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGgc                                                                                                    >  1:940257/1‑150 (MQ=255)
                                                    tCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTg                                                                                                                                           >  2:1229318/1‑109 (MQ=255)
                                                    tCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTg                                                                                                                                           <  1:1229318/109‑1 (MQ=255)
                                                     ccAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCa                                                                                                 >  1:1147890/1‑150 (MQ=255)
                                                      cAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAg                                                                                                >  2:1007410/1‑150 (MQ=255)
                                                            tcAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCCGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAggtg                                                                                          <  1:1357984/150‑1 (MQ=255)
                                                            tcAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAggtg                                                                                          >  1:1406615/1‑150 (MQ=255)
                                                             cAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAggtgg                                                                                         <  2:1406615/150‑1 (MQ=255)
                                                             cAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAggtgg                                                                                         <  2:629941/150‑1 (MQ=255)
                                                                       caaAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCa                                                                               >  1:538204/1‑150 (MQ=255)
                                                                          aGGGGCTTCGAGCAAAAGGACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGAGGCTGGCCAGCGCCCAGGATCCCTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGAt                                                                            <  2:912904/150‑1 (MQ=255)
                                                                              gCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCAc                                                                        <  2:1147890/150‑1 (MQ=255)
                                                                               cTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACg                                                                       >  1:751520/1‑150 (MQ=255)
                                                                                ttCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGc                                                                                                                                    <  2:737390/88‑1 (MQ=255)
                                                                                ttCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGc                                                                                                                                    >  1:737390/1‑88 (MQ=255)
                                                                                   gAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTAcg                                                                   >  1:1405550/1‑150 (MQ=255)
                                                                                     gCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATc                                                                                                                                      <  2:1006758/81‑1 (MQ=255)
                                                                                     gCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATc                                                                                                                                      >  1:1006758/1‑81 (MQ=255)
                                                                                     gCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTAcgcg                                                                 <  2:1315024/150‑1 (MQ=255)
                                                                                      cAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCgg                                                                <  1:1274608/150‑1 (MQ=255)
                                                                                         aaGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGga                                                             >  1:493779/1‑150 (MQ=255)
                                                                                            cACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACt                                                                                                                                            <  1:202228/68‑1 (MQ=255)
                                                                                            cACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACt                                                                                                                                            >  2:202228/1‑68 (MQ=255)
                                                                                               ccACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACgg                                                       >  2:685365/1‑150 (MQ=255)
                                                                                                 acaGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGcc                                                     >  2:298728/1‑150 (MQ=255)
                                                                                                          gCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTaa                                            >  2:11255/1‑150 (MQ=255)
                                                                                                                  tGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTc                                    <  1:1007410/150‑1 (MQ=255)
                                                                                                                   ggACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCt                                   <  1:1198002/150‑1 (MQ=255)
                                                                                                                    gACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTg                                  <  2:533928/150‑1 (MQ=255)
                                                                                                                     aCGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTgg                                 <  2:538204/150‑1 (MQ=255)
                                                                                                                        gAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAgg                                                                                                              >  1:1083745/1‑70 (MQ=255)
                                                                                                                        gAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAgg                                                                                                              <  2:1083745/70‑1 (MQ=255)
                                                                                                                           aCGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAg                           <  2:665321/150‑1 (MQ=255)
                                                                                                                            cGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAgg                          <  2:614576/150‑1 (MQ=255)
                                                                                                                               aaGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCa                       >  2:1283873/1‑150 (MQ=255)
                                                                                                                               aaGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCa                       <  1:685365/150‑1 (MQ=255)
                                                                                                                               aaGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCa                       >  2:1073190/1‑150 (MQ=255)
                                                                                                                                 tccTGGGCGCTGGCCAGCGTCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAg                                          <  2:1347213/127‑1 (MQ=255)
                                                                                                                                 tccTGGGCGCTGGCCAGCGTCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAg                                          >  1:1347213/3‑129 (MQ=255)
                                                                                                                                 gACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCAAc                     <  1:1283873/150‑1 (MQ=255)
                                                                                                                                 aaCTGGACGCTGGCCAGCGTCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCTGTAAAGGTGTTCTGGAACAAGGGCAAc                     <  2:458527/149‑1 (MQ=255)
                                                                                                                                    tGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAg                                                                              >  2:540710/1‑90 (MQ=255)
                                                                                                                                    tGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAg                                                                              <  1:540710/90‑1 (MQ=255)
                                                                                                                                       aCGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCAACATCAcc               <  2:751520/150‑1 (MQ=255)
                                                                                                                                           tGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCAACATCACCCTgg           >  1:523694/1‑150 (MQ=255)
                                                                                                                                            ggCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCAACATCACCCTggg          <  2:523694/150‑1 (MQ=255)
                                                                                                                                                   gcCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCAACATCACCCTGGGGTTTCgg   >  1:29258/1‑150 (MQ=255)
                                                                                                                                                   gcCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCAACATCACCCTGGGGTTTCgg   <  2:29258/150‑1 (MQ=255)
                                                                                                                                                    cccAGGATCACTGGAATCGCCAGACAATGACGCTGCCCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCAACATCACCCTGGGGTTTCGgc  >  2:675568/1‑150 (MQ=255)
                                                                                                                                                    cccAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCAACATCACCCTGGGGTTTCGgc  <  2:1405550/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GAAGCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCAACATCACCCTGGGGTTTCGGC  >  NC_002947/4300967‑4301264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑