Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,316,066 A→C 16.7% A35A (GCA→GCC)  PP_3788 → non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,316,0660AC16.7% 123.2 / 20.2 54A35A (GCA→GCC) PP_3788non‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base A (26/19);  new base C (4/5);  total (30/24)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

TCATCAGGGAATCAGTCTCGTACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACTGCGCTATCCCG  >  NC_002947/4315917‑4316208
                                                                                                                                                     |                                                                                                                                              
tcatcaGGGAATCAGTCTCGTACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCa                                                                                                                                                <  2:1295313/150‑1 (MQ=255)
   tcaGGGAATCAGTCTCGTACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCGTCGAATGCCGct                                                                                                                                             >  1:1334893/1‑149 (MQ=255)
   tcaGGGAATCAGTCTCGTACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCg                                                                                                                                             >  2:217222/1‑150 (MQ=255)
    caGGGAATCAGTCTCGTACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCTCTACCCATCGAATGCAGCgg                                                                                                                                            >  2:1301636/1‑150 (MQ=255)
      gggAATCAGTCTCGTACGCCACACAGCACGGAGCGCAGCATGAACCAGGCAGTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCa                                                                                                                                          >  2:414688/1‑150 (MQ=255)
       ggAATCAGTCTCGTACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCAt                                                                                                                                         >  2:426042/1‑150 (MQ=255)
              gTCTCGTACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCGTCGAATGCCGCTGCATTcgatgg                                                                                                                                  <  2:1334893/150‑4 (MQ=255)
              gTCTCGTACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCCGCTGCATTcgatgg                                                                                                                                  <  1:296476/150‑4 (MQ=255)
               tctcGTACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCGTCGAATGCCGCTGCATTcgatggg                                                                                                                                 <  1:477066/150‑5 (MQ=255)
               tctcGTACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAg                                                                                                                                 >  2:63955/1‑150 (MQ=255)
                          acacaGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCtt                                                                                                                      <  2:913725/150‑1 (MQ=255)
                            acaGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGa                                                                                                                    <  2:1419980/150‑1 (MQ=255)
                            acaGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGACATTCGACGAGGACCAGCGCTTGa                                                                                                                    >  1:1047689/1‑150 (MQ=255)
                                    gAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGc                                                                                                            >  1:627154/1‑150 (MQ=255)
                                       cgcAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGaa                                                                                                         <  1:865014/150‑1 (MQ=255)
                                            caTGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAg                                                                                                    <  1:426042/150‑1 (MQ=255)
                                               gAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGAt                                                                                                 <  1:998709/150‑1 (MQ=255)
                                                aaCCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATc                                                                                                <  2:627154/150‑1 (MQ=255)
                                                  ccAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGa                                                                                              >  2:1037221/1‑150 (MQ=255)
                                                        aTTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCt                                                                                        >  1:853165/1‑150 (MQ=255)
                                                           aCTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTcac                                                                                     >  2:41444/1‑150 (MQ=255)
                                                                     aCGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCt                                                                           >  2:737254/1‑150 (MQ=255)
                                                                          aGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCt                                                                      <  1:1037221/150‑1 (MQ=255)
                                                                             gcctgcctACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCg                                                                                                                                             >  1:708451/1‑76 (MQ=255)
                                                                             gcctgcctACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCg                                                                                                                                             <  2:708451/76‑1 (MQ=255)
                                                                                 gcctACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGaa                                                               <  1:414688/150‑1 (MQ=255)
                                                                                   ctACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGTAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACt                                                             >  1:104510/1‑150 (MQ=255)
                                                                                    tACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGaa                                                                                             <  2:1093978/117‑1 (MQ=255)
                                                                                    tACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGaa                                                                                             >  1:1093978/1‑117 (MQ=255)
                                                                                    tACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCt                                                                      >  2:665701/1‑140 (MQ=255)
                                                                                    tACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCt                                                                      <  1:665701/140‑1 (MQ=255)
                                                                                       cccGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCa                                                                   >  1:1313524/1‑140 (MQ=255)
                                                                                       cccGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCa                                                                   <  2:1313524/140‑1 (MQ=255)
                                                                                         cGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGCGCACTGATCGCCCTGCTGGCCACCAACGTCCGAACTGCTCACACTGCACGCTCAGCTGCATGAACTCGGCGt                                                       >  1:1236384/1‑150 (MQ=255)
                                                                                         cGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGt                                                       <  1:63955/150‑1 (MQ=255)
                                                                                            gCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTgcg                                                    >  1:491242/1‑150 (MQ=255)
                                                                                                  ctcGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCgg                                              <  1:1301636/150‑1 (MQ=255)
                                                                                                         gTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAAGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTAGGCGTGCGCAGCGGGGAtgtg                                       >  2:85201/1‑150 (MQ=255)
                                                                                                                 cATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGt                               <  1:41444/150‑1 (MQ=255)
                                                                                                                  aTCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCa                                                                   <  1:515019/113‑1 (MQ=255)
                                                                                                                  aTCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCa                                                                   >  2:515019/1‑113 (MQ=255)
                                                                                                                       gCGTATCATCCACCGCTACCCATCGAATGCAGCGACATTCGACGAGGACCAGCGCTTGACCTACAGCGAAATAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGc                         <  2:1047689/150‑1 (MQ=255)
                                                                                                                            tcatcCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAg                    >  2:426970/1‑150 (MQ=255)
                                                                                                                               tcCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGc                 <  1:737254/150‑1 (MQ=255)
                                                                                                                                 cACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGCaa               >  2:203740/1‑150 (MQ=255)
                                                                                                                                   ccGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACt             >  1:820790/1‑150 (MQ=255)
                                                                                                                                        cctcgtCGAATGCCGCTGCATTCGATGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACTGCGCt        >  2:1070874/6‑150 (MQ=255)
                                                                                                                                          tcgtCGAATGCCGCTGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGa                                           <  1:423518/110‑1 (MQ=255)
                                                                                                                                          tcgtCGAATGCCGCTGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGa                                           >  2:423518/4‑113 (MQ=255)
                                                                                                                                            gtCGAATGCCGCTGCATTCGATGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGt                   >  1:1336926/2‑135 (MQ=255)
                                                                                                                                            gtCGAATGCCGCTGCATTCGATGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGt                   <  2:1336926/134‑1 (MQ=255)
                                                                                                                                            aTCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACTGCGCTATcc    >  1:520923/1‑150 (MQ=255)
                                                                                                                                             tCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAAt                           <  1:1262441/126‑1 (MQ=255)
                                                                                                                                             tCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAAt                           >  2:1262441/1‑126 (MQ=255)
                                                                                                                                             tCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTA‑AGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACTGCGCTATCCcg  >  2:920868/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                              
TCATCAGGGAATCAGTCTCGTACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACTGCGCTATCCCG  >  NC_002947/4315917‑4316208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑