Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,316,712 A→G 11.4% M251V (ATG→GTG)  PP_3788 → non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,316,7120AG11.4% 101.9 / 4.4 44M251V (ATG→GTG) PP_3788non‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base A (19/20);  new base G (2/3);  total (21/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01

GGCCCTGAAAACCCAGGCCTCGTTCGACCCCTCGATCCAGGAGGCCGTGCTGCCTTTTTTTTCCGCCGGCGCCGTGTTCGTGCCTGACCACAACCG‑AGTCAACTTTCCCAATTACCTGTCGGCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATGGTACCCAGCCACCTGCAGCATCTTCTCGCCAGCCCGGCAATCAATGCCTGCCAGCACCTCAGGCATATCGTTTGCTGCGGAGAACCTTGGGGAGTTGAGTTGATCAGTGCGCTTCATCAGCGCTTGCCGAACTGC  >  NC_002947/4316564‑4316858
                                                                                                                                                     |                                                                                                                                                  
ggCCCTGAAAACCCAGGCCTCGTTCGACCCCTCGATCCAGGAGGCCGTGCTGCCTTTTTTTTCCGCCGGCGCCGTGTTCGTGCCTGACCACAACCG‑AGTCAACTTTCCCAAACACCTGTCGGCCTGCATCGCTGAGCACGGTGTCACCAt                                                                                                                                                   <  1:473458/150‑1 (MQ=255)
  cccTGAAACCCCAGGCCTCTTTCGACCCCTCGATCCAGGAGGCCGTGCTGCCTTTTTTTTCCGCCGGCGCCGTGTTCGTGCCTGACCACAACCG‑AGTCAACTTTCCCAATTACCTGTCGGCCTGCATCGCTGAGCATGGTGACACCGTGc                                                                                                                                                 <  2:616512/150‑1 (MQ=255)
    cTGAAAACCCAGGCCTCGTTCGACCCCTCGATCCAGGAGGCCGTGCTGCCTTTTTTTTCCGCCGGCGCCGTGTTCGTGCCTGACCACAACCG‑AGTCAACTTTCCCAATTACGTGGCGCCCTGCATCGCTGATCACGGTGTCACCATGCtc                                                                                                                                               >  1:454004/1‑150 (MQ=255)
          aCCCAGGCCTCGTTCGACCCCTCGATCCAGGAGGCCGTGCTGCCTTTTTTTTCCGCCGGCGCCGTGTTCGTGCCTGACCACAACCG‑AGTCAACTTTCCCAATTACCTGTCGGCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATg                                                                                                                                         <  1:148617/150‑1 (MQ=255)
          aCCCAGGCCTCGTTCGACCCCTCGATCCAGGAGGCCGTGCTGCCTTTTTTTTCCGCCGGCGCCGTGTTCGTGCCTGACCACAACCG‑AGTCAACTTTCCCAATTACCTGTCGGCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATg                                                                                                                                         <  1:33260/150‑1 (MQ=255)
              aGGCCTCGTTCGACCCCTCGATCCAGGAGGCCGTGCTGCCTTTTTTTTCCGCCGGCGCCGTGTTCGTGCCTGACCACAACCG‑AGTCAACTTTCCCAATTACCTGTCGGCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATGGTAc                                                                                                                                     >  1:408774/1‑150 (MQ=255)
                 ccTCGTTCGACCCCTCGATCCAGGAGGCCGTGCTGCCTTTTTTTTCCGCCGGCGCCGTGTTCGTGCCTGACCACAACCG‑AGTCAACTTTCCCAATTACCTGTCGGCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATGGTACCCa                                                                                                                                  <  1:1324011/150‑1 (MQ=255)
                      ttCGACCCCTCGATCCAGGAGGCCGTGCTGCCTTTTTTTTCCGCCGGCGCCGTGTTCGTGCCTGACCACAACCG‑AGTCAACTTTCCCAATTACCTGTCGGCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATGGTACCCAGCCAc                                                                                                                             <  2:997232/150‑1 (MQ=255)
                            cccTCGATCCAGGAGGCCGTGCTGCCTTTTTTTTCCGCCGGCGCCGTGTTCGTGCCTGACCACAACCG‑AGTCAACTTTCCCAATTACCTGTCGGCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATGGTACCCAGCCACCTgcag                                                                                                                       >  2:428027/1‑150 (MQ=255)
                             ccTCGATCCAGGAGGCCGTGCTGCCTTTTTTTTCCGCCGGCGCCGTGTTCGTGCCTGACCACAACCG‑AGTCAACTTTCCCAATTACCTGTCGGCCTGCATCGCTGAGCATGGTGACACCGTGCtcagc                                                                                                                                            >  2:1370203/1‑126 (MQ=255)
                             ccTCGATCCAGGAGGCCGTGCTGCCTTTTTTTTCCGCCGGCGCCGTGTTCGTGCCTGACCACAACCG‑AGTCAACTTTCCCAATTACCTGTCGGCCTGCATCGCTGAGCATGGTGACACCGTGCtcagc                                                                                                                                            <  1:1370203/128‑3 (MQ=255)
                                       ggaggCCGTGCTGCCTTTTTTTTCCGCCGGCGCCGTGTTTGTGCCTGACCACAACTG‑AGTCAGCTTTTCCAATTACCTGTCGGCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATGTTACCCAGCCCCCTGCAGCATCTTCTCGc                                                                                                            >  2:746205/1‑150 (MQ=255)
                                          ggCCGTGCTGCCTTTTTTTTCCGCCGGCGCCGTGTTCGTGCCTGACCACAACCG‑AGTTAACTTTCCCAATTACCTGTCGGCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATGGTACCCAGCCACCTGCAGCATCTTCTCGCCAg                                                                                                         >  1:263354/1‑150 (MQ=255)
                                                    ccTTTTTTTTCCGCCGGCGCCGTGTTCGTGCCTGAACAAACTCGAACTCGA‑AAACCCAATTACCTGTCGGTCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATGGTACCCAGCAACCTGCAGCATCTTCTCGCCAGCCCGGcaatc                                                                                               >  1:603047/1‑150 (MQ=255)
                                                      ttttttttCCGCCGGCGCCGTGTTCGTGCCTGACCACAACCG‑AGTCAACTTTCCCAATTACCTGTCGGCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATGGTACCCAGCCACCTGCAGCATCTTCTCGCCAGCCCGGcaatc                                                                                               <  2:191396/148‑1 (MQ=255)
                                                      ttttttttCCGCCGGCGCCGTGTTCGTGCCTGACCACAACCG‑AGTCAACTTTCCCAATTACCTGTCGGCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATGGTACCCAGCCACCTGCAGCATCTTCTCGCCAGCCCGGcaatc                                                                                               >  1:191396/1‑148 (MQ=255)
                                                              ccgccgGCGCCGTGTTCGTGCCTGACCACAACCG‑AGTCAACTTTCCCAATTACCTGTCGGCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATGGTACCCAGCCACCTGCAGCATCTTCTCGCCAGCCCGGCAATCAAtgcct                                                                                        <  2:48441/147‑1 (MQ=255)
                                                              ccgccgGCGCCGTGTTCGTGCCTGACCACAACCG‑AGTCAACTTTCCCAATTACCTGTCGGCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATGGTACCCAGCCACCTGCAGCATCTTCTCGCCAGCCCGGCAATCAAtgcct                                                                                        >  1:48441/1‑147 (MQ=255)
                                                                              cGTGCCTGACCACAACCG‑AGTCAACTTTCCCAATTACCTGTCTGCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATGGTACCCAGCCACCTGCAGCATCTTCTCGCCAGCCCGGCAATCAATGCCTGCCAGCACCTCAGGCatat                                                                     <  1:970863/150‑1 (MQ=255)
                                                                               gTGCCTGACCACAACCG‑AGTCAACTTTCCCAATTACCTGTCGGCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATGGTACCCAGCCACCTGCAGCATCTTCTCGCCAGCCCGGCAATCAATGCCTGCCAGCACCTCAGGCATATc                                                                    >  1:438772/1‑150 (MQ=255)
                                                                                  ccTGACCACAACCG‑AGTCAACTTTCCCAATTACCTGTCGGCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATGGTACCCAGCCACCTGCAGCATCTTCTCGCCAGCCCGGCAATCAATGCCTGCCAGCACCTCAGGCATATCGtt                                                                 <  2:740742/150‑1 (MQ=255)
                                                                                     gACCACAACCG‑AGTCAACTTTCCCAATTACCTGTCGGCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATGGTACCCAGCCACCTGCAGCATCTTCTCGCCAGCCCGGCAATCAATGCCTGCCAGCACCTCAGGCATATCGTTtgc                                                              >  2:1060856/1‑150 (MQ=255)
                                                                                        cacaACCG‑AGTCAACTTTCCCAATTACCTGTCGGCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATGGTACCCAGCCAc                                                                                                                             <  1:1378904/84‑1 (MQ=255)
                                                                                        cacaACCG‑AGTCAACTTTCCCAATTACCTGTCGGCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATGGTACCCAGCCAc                                                                                                                             >  2:1378904/1‑84 (MQ=255)
                                                                                         acaACCG‑AGTCAACTTTCCCAATTACCTGTCGGCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATGGTACCCAGCCACCTGCAGCATCTTCTCGCCAGCCCGGCAATCAATGCCTGCCAGCACCTCAGGCATATCGTTTGCTGCg                                                          <  2:408774/150‑1 (MQ=255)
                                                                                         acaACCG‑AGTCAACTTTCCCAATTACCTGTCGGCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATGGTACCCAGCCACCTGCAGCATCTTCTCGCCAGCCCGGCAATCAATGCCTGCCAGCACCTCAGGCATATCGTTTGCTGCg                                                          <  1:428027/150‑1 (MQ=255)
                                                                                              cG‑AGTCAACTTTCCCAATTACCTGTCGGCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATGGTACCCAGCCACCTGCAGCATCTTCTCGCCAGCCCGGCAATCAATGCCTGCCAGCACCTCAGGCATATCGTTTGCTGCGGAGaa                                                     <  1:796929/150‑1 (MQ=255)
                                                                                                   tCAACTTGCCCAATTACCTGTCGGCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATGGTACCCAGCCACCTGCAGCATCTTCTCGCCAGCCCGGCAATCAATGCCTGCCAGCACCTCCGGCATATCGTTTGCTGCGGAGAACCtt                                                 <  2:229406/150‑1 (MQ=255)
                                                                                                     aaCTTTCCCAATTACCTGTCGGCCTGCATAGCTGCGCAGAGTGGCACCATGCTCATCATGGTACCCAGCCACCTGCAGCATCTTCTCGCCAGCCCGGCAATCAATGCCTGCCAGCACCTCAGGCATATCGTTTGCTGCGGAGAACCTTgg                                               >  1:983624/1‑150 (MQ=255)
                                                                                                     aaCTGTCCCAATTACCTGTCGGCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATGGTACCCAGCCACCTGCAGCATCTTCTCGCCAGCCCGGCAATCAATGCCTGCCAGCACCTCAGGCATATCGTTTGCTGCGGAGAACCTTgg                                               <  2:603047/150‑1 (MQ=255)
                                                                                                      aCTTTCCCAATTGCCTGCCGGCCTGCACCGCCGAGCACGGTGTCCCCATGCTCATCATGGTACCCAGCCACCTGCAGCCTCTTCTCGCCAGCCCGGCAATCAATGCCTGCCAGCACCTCAGGCATATCGTTTGCTGCGGAGAACCTTggg                                              <  1:622231/150‑1 (MQ=255)
                                                                                                      aCTTTCCCAATTACCTGTCGGCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATGGTACCCAGCCACCTGCAGCATCTTCTCGCCAGCCCGGCAATCAATGCCTGCCAGCACCTCAGGCATATCGTTTGCTGCGGAGAACCTTggg                                              <  1:1060856/150‑1 (MQ=255)
                                                                                                      aCTTTCCCAATTACCTGTCGGCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATGGTACCCAGCCACCTGCAGCATCTTCTCGCCAGCCCGGCAATCAATGCCTGCCAGCACCTCAGGCATATCGTTTGCTGCGGAGAACCTTggg                                              >  2:787452/1‑150 (MQ=255)
                                                                                                      aCTTTCCCAATTACCTGTCGGCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATGGTACCCAGCCACCTGCAGCATCTTCTCGCCAGCCCGGCAATCAATGCCTGCCAGCACCTCAGGCATATCGTTTGCTGCGGAGAACCTTggg                                              >  1:532344/1‑150 (MQ=255)
                                                                                                        tttCCCAATTACCTGTCGGCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATGGTACCCAGCCACCTGCAGCATCTTCTCGCCAGCCCGGCAATCAATGCCTGCCAGCACCTCAGGCATATCGTTTGCTGCGGAGAACCTTGGGga                                            >  2:1425098/1‑150 (MQ=255)
                                                                                                              aaTTACCTGTCGGCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATGGTACCCAGCCACCTGCAGCATCTTCTCGCCAGCCCGGCAATCAATGCCTGCCAGAAATCCAAGCATATCGTTTGCTGCGGAGAACCTTGGGgagttgag                                      >  1:1085439/1‑150 (MQ=255)
                                                                                                                     tGTCGGCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATGGTACCCAGCCACCTGCAGCATCTTCTCGCCAGCCCGGCAATCAATGCCTGCCAGCACCTCAGGCATATCGTTTGCTGCGGAGAACCTTGGGGAGTTGAGTTGATCa                               <  2:983624/150‑1 (MQ=255)
                                                                                                                       tCGGCCTGCACCGCTGACAACGGTGTCACCATGCTCATCATGGTACCCAGCCACCTGCAGCATCTTCTCGCCAGCCCGGCAATCAATGCCTGCCAGCACCTCAGGCATATCGTTTGCTGCGGAGAACCTTGGGGAGTTGAGTTGATCAGt                             <  1:17159/150‑1 (MQ=255)
                                                                                                                          gCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATGGTACCCAGCCACCTGCAGCATCTTCTCGCCAGCCCGGCAATCAATGCCTGCCAGCACCTCAGGCATATCGTTTGCTGCGGAGAACCtt                                                 <  1:290161/127‑1 (MQ=255)
                                                                                                                          gCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATGGTACCCAGCCACCTGCAGCATCTTCTCGCCAGCCCGGCAATCAATGCCTGCCAGCACCTCAGGCATATCGTTTGCTGCGGAGAACCtt                                                 >  2:290161/1‑127 (MQ=255)
                                                                                                                                catgatGAGCATGGTGACACCGTGCTCATCATGGTACCCAGCCACCTGCAGCATCTTCTCGCCAGCCCGGCAATCAATGCCTGCCAGCACCTCAGGCATATCGTTTGCTGCGGAGAACCTTgg                                               <  2:585104/118‑1 (MQ=255)
                                                                                                                                catgatGAGCATGGTGACACCGTGCTCATCATGGTACCCAGCCACCTGCAGCATCTTCTCGCCAGCCCGGCAATCAATGCCTGCCAGCACCTCAGGCATATCGTTTGCTGCGGAGAACCTTgg                                               >  1:585104/6‑123 (MQ=255)
                                                                                                                                    cTGAGCACGGTGTCACCATGCTCATCATGGTACCCAGCCACCTGCAGCATCTTCTCGCCAGCCCGGCAATCAATGCCTGCCAGCACCTCAGGCATATCGTTTGCTGCGGAGAACCTTGGGGAGTTGAGTTGATCAGTGCGCTTCATCAgc                >  2:22577/1‑150 (MQ=255)
                                                                                                                                                  aCCATGCTCATCATGGTACCCAGCCACCTGCAGCATCTTCTCGCCAGCCCGGCAATCAATGCCTGCCAGCACCTCAGGCATATCGTTTGCTGCGGAGAACCTTGGGGAGTTGAGTTGATCAGTGCGCTTCATCAGCGCTTGCCGAACTGc  >  1:635853/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GGCCCTGAAAACCCAGGCCTCGTTCGACCCCTCGATCCAGGAGGCCGTGCTGCCTTTTTTTTCCGCCGGCGCCGTGTTCGTGCCTGACCACAACCG‑AGTCAACTTTCCCAATTACCTGTCGGCCTGCATCGCTGAGCACGGTGTCACCATGCTCATCATGGTACCCAGCCACCTGCAGCATCTTCTCGCCAGCCCGGCAATCAATGCCTGCCAGCACCTCAGGCATATCGTTTGCTGCGGAGAACCTTGGGGAGTTGAGTTGATCAGTGCGCTTCATCAGCGCTTGCCGAACTGC  >  NC_002947/4316564‑4316858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑