| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,327,297 | A→T | 9.6% | N74I (AAC→ATC) ‡ | PP_3797 → | membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,327,297 | 0 | A | T | 9.6% | 184.7 / 10.0 | 63 | N74I (AAC→ATC) ‡ | PP_3797 | membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (25/31); new base T (3/3); total (28/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.02e-01 | |||||||||||
TTCTTTGTCCTGATCGTCTGTTTCACCGGCATGCTCGCCGTGGTCAGTCAGGAAATCGTCTGGCTCGCCGATCCGGCCGCACGCGCCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCGGTAAAAGCCAATGTCACCCTGCCTGACGGCACCAGCCCGACGCTGTATGTAAACCCCTACACCGGAACCATTCAGGGCAGGAC > NC_002947/4327152‑4327441 | ttCTTTGTCCTGATCGTCTGTTTCACCGGCATGCTCGCCGTGGTCAGTCAGGAAATCGTCTGGCTCGCCGATCCGGCCGCACGCGCCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAg > 1:296145/1‑150 (MQ=255) tCTTTGTCCTGATCGTCTGTTTGGCCGGCATGCTCGCCGTGGTCAGTCAGGAAATCGTCTGGCTCGCCGATCCGGCCGCACGCGCTAACAAGCCAGACGGGGACACCGAGCGCATGAGCTTTCAGCAAGTACTGGAGGGCTTGAACaatg < 2:1235399/150‑3 (MQ=255) tCTTTGTCCTGATCGTCTGTTTCACCGGCATGCTCGCCGTGGTCAGTCAGGAAATCGTCTGGCTCGCCGATCCGGCCGCACGCGCCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGATGCCTTGAACAAgg < 1:806970/150‑1 (MQ=255) tGTCCTGATCGTCTGTTTCACCGGCATGCTCGCCGTGGTCAGTCAGGAAATCGTCTGGCTCGCCGATCCGGCCGCACGCGCCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGTTCAAGGcctc < 2:1001080/150‑3 (MQ=255) tGATCGTCTGTTTCACCGGCATGCTCGCCGTGGTCAGTCAGGAAATCGTCTGGCTCGCCGATCCGGCCGCACGCGCCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCCGGTACAGGGGGCCTTGAACAAGGCCGAGCCgg > 2:180191/1‑150 (MQ=255) tGATCGTCTGTTTCACCGGCATGCTCGCCGTGGTCAGTCAGGAAATCGTCTGGCTCGCCGATCCGGCCGCACGCGCCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCgg > 1:379908/1‑150 (MQ=255) gATCGTCTGTTTCACCGGCATGCTCGCCGTGGTCAGTCAGGAAATCGTCTGGCTCGCCGATCCGGCCGCACGCGCCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGa > 1:1312106/1‑150 (MQ=255) tCTGTTTCACCGGCATGCTCGCCGTGGTCAGTCAGGAAATCGTGTGGCTCGCCGATCCGGCCGCACGCGCCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACAtgg < 1:866342/150‑1 (MQ=255) tttCACCGGCATGCTCGCCGTGGTCAGTCAGGAAATCGTCTGGCTCGCCGATCCGGCCGCACGCGCCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACAtggtggt > 1:1417109/1‑150 (MQ=255) ttcagtggCATGGTCGTCCGGGGCAGTCAGGAAGTTGTCTAGCGCGCGACGCCGTCCCCACGCGCCAACACGTCAGACGCGGACACCGAGCGCATGAGCTGTCAGCAGGTACTGGAGGCCTTGACCAAGGCAAAGCCGGACAtggtgatg < 2:277780/144‑1 (MQ=255) cACCGGCATGCTCGCCGTGGTCAGTCAGGAAATCGTCTGGCTCGCCGATCCGGCCGCACGCGCCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGa < 1:915076/150‑1 (MQ=255) ggCATGCTCGCCGTGGTCAGTCAGGAAATCGTCTGGCTCGCCGATCCGGCCGCACGCGCCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAgcgc < 1:940448/150‑1 (MQ=255) tGCTCGCCGTGGTCAGTCAGGCAATCGTATGGCTGTCCTAGCCGTTCGCACGCGCCAACAAGCCAGACGCGGACACCGGGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGa < 2:12998/150‑1 (MQ=255) tGCTCGCCGTGGTCAGTCAGGAAATCGGCTGGCTCGCCGATCCGGCCGCACGCGCCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGa < 1:229817/150‑1 (MQ=255) gCCGTGGTCAGTCAGGAAATCGTCTGGCTCGCCGATCCGGCCGCACGCGCCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGagccag > 1:241006/1‑150 (MQ=255) tcagtcagGAAATCGTCTGGCTCGCCGATCCGGCCGCACGCGCCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATg < 2:241006/150‑1 (MQ=255) gtcagGAAATCGTCTGGCTCGCCGATCCGGCCGCACGCGCCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGTTCAAGGc < 1:124699/106‑1 (MQ=255) gtcagGAAATCGTCTGGCTCGCCGATCCGGCCGCACGCGCCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGTTCAAGGc > 2:124699/1‑106 (MQ=255) tcagGCAATCGTCTGGCTCGCCGATCCGGCCGCACGCGCCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGcc < 1:1155835/106‑1 (MQ=255) tcagGAAATCGTCTGGCTCGCCGATCCGGCCGCACGCGCCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGcc > 2:1155835/1‑106 (MQ=255) ggAAATCGTCTGGCTCGCCGATCCGGCCGCACGCGCCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCa > 2:1116617/1‑150 (MQ=255) tCTGGCTCGCCGATCCGGCCGCACGCGCCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTc < 1:516022/139‑1 (MQ=255) tCTGGCTCGCCGATCCGGCCGCACGCGCCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTc > 2:516022/1‑139 (MQ=255) gCTCGCCGATCCGGCCGCACGCGCCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATg < 1:61492/131‑1 (MQ=255) gCTCGCCGATCCGGCCGCACGCGCCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATg > 2:61492/1‑131 (MQ=255) cTCGCCGATCCGGCCGCACGCGCCAACAAGCCAGACGCGGACACCGCGCGCATGAGGGTGCAGCACGTCCTGTCTGCCGTGTACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCGGTaaaa < 2:337858/150‑1 (MQ=255) tCGCCGATCCGGCCGCACGCGCCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAACAAGGCGGATGGTACGGACTACGCGGAaaaac > 2:1014497/1‑149 (MQ=255) aTCCGGCCGCACGCGCCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGTTCAAGGcctcc > 1:1217004/1‑83 (MQ=255) aTCCGGCCGCACGCGCCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGTTCAAGGcctcc < 2:1217004/86‑4 (MQ=255) ggCCGCACGCGCCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCGGTAAAAGCCAATGTCAc > 1:984341/1‑150 (MQ=255) gCCGCACGCGCCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCGGTAAAAGCCAATGTCAcc < 2:1312106/150‑1 (MQ=255) cgcgCCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCGGTAAAAGCCAATGTCACcctgcct < 1:180191/150‑1 (MQ=255) gcgcCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCg > 1:1393790/1‑77 (MQ=255) gcgcCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCg < 2:1393790/77‑1 (MQ=255) gcCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACAtggtg < 2:180936/84‑1 (MQ=255) gcCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACAtggtg > 1:180936/1‑84 (MQ=255) ccAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCGGTAAAAGCCAATGTCACCCTGCCTGACg < 1:1331538/150‑1 (MQ=255) caacaaGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCgg > 1:119217/1‑122 (MQ=255) caacaaGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCgg < 2:119217/122‑1 (MQ=255) caaGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCGGTAAAAGCCAATGTCACCCTGCCTGACGGCAc < 1:1014497/150‑1 (MQ=255) gCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGACGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCGGTAAAAGCCAATGTCACCCTGCCTGACGGCACCAg < 2:379908/150‑1 (MQ=255) ccAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCGGTAAAAGCCAATGTCACCCTGCCTGACGGCACCAGc > 2:994952/1‑150 (MQ=255) cAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCGGTAAAAGCCAATGTCACCCTGCCTGACGGCACCAGcc < 1:1116617/150‑1 (MQ=255) gACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCGGTAAAAGCCAATGTCACCCTGCCTGACGGCACCAGCCCGACGCtg > 1:1213875/1‑150 (MQ=255) acCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGACACGGAGAAATGTTTCGCACTTCGCGGTAAAAGCCAATGTCACCCTCCCTGACGGACCCAGGCCGCCGCtttat > 1:88045/1‑150 (MQ=255) cGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCGGTAAAAGCCAAt < 2:218463/112‑1 (MQ=255) cGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCGGTAAAAGCCAAt > 1:218463/1‑112 (MQ=255) cgcATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCGGTAAAAGCCAATGTCACCCTGCCTGACGGCACCAGCCCGACGCTGTATGTAAAc < 1:994952/150‑1 (MQ=255) aTGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCGGTAAAAGCCAATGTCACCCTGCCTGACGGCACCa < 2:64699/127‑1 (MQ=255) aTGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCGGTAAAAGCCAATGTCACCCTGCCTGACGGCACCa > 1:64699/1‑127 (MQ=255) aTGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCGGTAAAAGCCAATGTCACCCTGCCTGACGGCACCACACCTAGGTTGGAGGTAAAcccc < 2:1417109/150‑1 (MQ=255) tttCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCGGTAAAAGCCAATGTCACCCTGCCTGACGGCAc < 1:774204/119‑1 (MQ=255) tttCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCGGTAAAAGCCAATGTCACCCTGCCTGACGGCAc > 2:774204/1‑119 (MQ=255) tttCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCGGTAAAAGCCAATGTCACCCTGCCTGACGGCACCAGCCCGACGCtgtat < 1:70748/135‑1 (MQ=255) tttCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCGGTAAAAGCCAATGTCACCCTGCCTGACGGCACCAGCCCGACGCtgtat > 2:70748/1‑135 (MQ=255) agcagGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCGGTAAACCCCAATGTCACCATGATTGATGGGTCCATCCCGACGCTGTATGTAAACCCCTACACCCGaa < 2:49692/150‑1 (MQ=255) gTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGGGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCGGTAAAAGCCAATGTCACCCTGCCTGACGGCACCAGCCCGACGCTGTATGTAAACCCCTACACCGGAACCAtt > 1:609041/1‑150 (MQ=255) tcggCCTTGTTCAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCGGTAAAAGCCAATGTCACCCTGCCTGACGGCACCAGCCCGACGCTGTATGTAAACCCCTACACCGGAACCATTCAGGGc > 1:1105012/3‑150 (MQ=255) aGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCGGTAAAAGCCAATGTCACcctgcct > 1:668140/1‑95 (MQ=255) aGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCGGTAAAAGCCAATGTCACcctgcct < 2:668140/95‑1 (MQ=255) aGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCGGTAAAAGCCAATGTCACCCTGCCTGACGGCACCAGCCCGACGCTGTATGTAAACCCCTACACCGGAACCATTCAGGGCa < 2:296145/150‑1 (MQ=255) aGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCGGTAAAAGCCAATGTCACCCTGCCTGACGGCACCAGCCCGACGCTGTATGTAAACCCCTACACCGGAACCATTCAGGGCa < 1:1130815/150‑1 (MQ=255) cTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCGGTAAAAGCCAATGTCACCCTGCCTGACGGCACCAGCCCGACGCTGTATGTAAACCCCTACACCGGAACCATTCAGGGCAGGAc > 2:25047/1‑150 (MQ=255) aaCAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCGGTAAAAGCCAATGTCACcctgcc > 2:889799/1‑86 (MQ=255) aaCAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCGGTAAAAGCCAATGTCACcctgcc < 1:889799/86‑1 (MQ=255) | TTCTTTGTCCTGATCGTCTGTTTCACCGGCATGCTCGCCGTGGTCAGTCAGGAAATCGTCTGGCTCGCCGATCCGGCCGCACGCGCCAACAAGCCAGACGCGGACACCGAGCGCATGAGCTTTCAGCAGGTACTGGAGGCCTTGAACAAGGCCGAGCCGGACATGGTGGTGGAGCGCCTGAGCCAGCCGGATGGTTCGCACTTCGCGGTAAAAGCCAATGTCACCCTGCCTGACGGCACCAGCCCGACGCTGTATGTAAACCCCTACACCGGAACCATTCAGGGCAGGAC > NC_002947/4327152‑4327441 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |