Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,399,566 A→T 6.9% I123N (ATT→AAT)  PP_3878 ← minor capsid protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,399,5660AT 6.9% 174.2 / 4.1 58I123N (ATT→AAT) PP_3878minor capsid protein C
Reads supporting (aligned to +/- strand):  ref base A (23/31);  new base T (2/2);  total (25/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.98e-01

GCAAGCAGACGCAATCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGG  >  NC_002947/4399418‑4399709
                                                                                                                                                    |                                                                                                                                               
gCAAGCAGACGCAATCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAAt                                                                                                                                                <  1:1345009/150‑1 (MQ=255)
  aaGCAGACGCAATCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAAtgt                                                                                                                                              >  2:252589/1‑150 (MQ=255)
    gCAGACGCAATCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATgtgt                                                                                                                                            >  1:580710/1‑150 (MQ=255)
      aGACGCAATCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAAGGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGGCAATGTCGAGCACAATGTGTTc                                                                                                                                          <  2:635573/150‑1 (MQ=255)
            aaTCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTc                                                                                                                                          >  2:944523/1‑144 (MQ=255)
            aaTCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTc                                                                                                                                          <  1:944523/144‑1 (MQ=255)
            aaTCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCAt                                                                                                                                    <  1:1066531/150‑1 (MQ=255)
             aTCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATg                                                                                                                                   <  1:131998/150‑1 (MQ=255)
              tCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCATGGTCGAACACATtgtgc                                                                                                                                            >  2:500535/1‑139 (MQ=255)
              tCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCATGGTCGAACACATtgtgc                                                                                                                                            <  1:500535/140‑2 (MQ=255)
                  gTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTc                                                                                                                              >  2:1232704/1‑150 (MQ=255)
                    aaCCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAg                                                                                                                            >  1:269687/1‑150 (MQ=255)
                    aaCCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACAATGGGGTCAg                                                                                                                            >  1:874458/1‑150 (MQ=255)
                       ccccTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATggg                                                                                                                                 >  2:47133/1‑142 (MQ=255)
                       ccccTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATggg                                                                                                                                 <  1:47133/142‑1 (MQ=255)
                       ccccTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGa                                                                                                                         >  2:163215/1‑150 (MQ=255)
                       ccccTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACAATGGGGTCAGTGa                                                                                                                         <  2:874458/150‑1 (MQ=255)
                         ccTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTg                                                                                                                          <  2:1324648/147‑1 (MQ=255)
                         ccTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTg                                                                                                                          >  1:1324648/1‑147 (MQ=255)
                           tGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGc                                                                                                                     <  1:252589/150‑1 (MQ=255)
                                 cGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTAtt                                                                                                               <  1:163215/150‑1 (MQ=255)
                                   ccATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCa                                                                                                             <  2:580710/150‑1 (MQ=255)
                                   ccATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCa                                                                                                             >  2:481748/1‑150 (MQ=255)
                                    cATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAg                                                                                                            >  2:475254/1‑150 (MQ=255)
                                     aTGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAAt                                                                                                                                                >  2:661029/1‑113 (MQ=255)
                                     aTGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAAt                                                                                                                                                <  1:661029/113‑1 (MQ=255)
                                      tGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAggg                                                                                                          <  1:1352457/150‑1 (MQ=255)
                                       gAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGgc                                                                                                         >  2:508322/1‑150 (MQ=255)
                                          gTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGgctgc                                                                                                      <  1:989511/150‑1 (MQ=255)
                                               ccAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCa                                                                                                 >  1:1256664/1‑150 (MQ=255)
                                                    ccGGTGATTGGGTTGATCTTGGTCGCAGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCGGGGCTGTCAATGTCGACCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCGAGCAGCCCt                                                                                            <  2:511514/150‑1 (MQ=255)
                                                           ttGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGGGTCTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCGAGCAGCCCtgaataag                                                                                     <  2:1046523/150‑8 (MQ=255)
                                                            tGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCt                                                                                    <  1:475254/150‑1 (MQ=255)
                                                               tttGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTAtt                                                                                                               >  2:110185/1‑120 (MQ=255)
                                                               tttGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTAtt                                                                                                               <  1:110185/120‑1 (MQ=255)
                                                                  gATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGAt                                                                                                                        <  2:961177/108‑1 (MQ=255)
                                                                  gATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGAt                                                                                                                        >  1:961177/1‑108 (MQ=255)
                                                                     cTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGt                                                                           <  2:269687/150‑1 (MQ=255)
                                                                        ggTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGtctc                                                                        <  1:508322/150‑1 (MQ=255)
                                                                                cccGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGt                                                                <  2:357375/150‑1 (MQ=255)
                                                                                 ccGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCGAGCAGcc                                                                                              >  1:1177043/1‑119 (MQ=255)
                                                                                 ccGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCGAGCAGcc                                                                                              <  2:1177043/119‑1 (MQ=255)
                                                                                      tGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGt                                                          <  1:1232704/150‑1 (MQ=255)
                                                                                       gTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTg                                                         <  1:761934/150‑1 (MQ=255)
                                                                                         ccgccgCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGc                                                       <  1:481748/150‑1 (MQ=255)
                                                                                              gccAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTGACTCAGGGCTGCTCGCAGCCCTTCATAGCTGGGAATGGTCTCGCAGGCGTTCAAGTGGCTGAcg                                                  >  2:625676/1‑150 (MQ=255)
                                                                                               ccAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCcgc                                                 >  2:1400394/1‑150 (MQ=255)
                                                                                                           cgcCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGaa                                     <  2:1256664/150‑1 (MQ=255)
                                                                                                              cGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGc                                  >  1:267082/1‑150 (MQ=255)
                                                                                                                    cgcTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGa                            <  1:1205868/150‑1 (MQ=255)
                                                                                                                      cTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACg                          <  2:1065043/150‑1 (MQ=255)
                                                                                                                                    gTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCcac            <  2:267082/150‑1 (MQ=255)
                                                                                                                                        aTGTCGCGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACAccc        >  2:420126/1‑150 (MQ=255)
                                                                                                                                           tCGAACACATTGTGCTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCa                                <  1:21235/123‑1 (MQ=255)
                                                                                                                                           tCGAACACATTGTGCTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCa                                >  2:21235/1‑123 (MQ=255)
                                                                                                                                             gAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACgg                         <  2:1415121/128‑1 (MQ=255)
                                                                                                                                             gAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACgg                         >  1:1415121/1‑128 (MQ=255)
                                                                                                                                              aGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTgg  >  2:563190/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
GCAAGCAGACGCAATCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGG  >  NC_002947/4399418‑4399709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑