| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,458,791 | A→T | 8.3% | intergenic (+39/‑229) | pcaJ → / → trkH‑I | 3‑oxoadipate CoA‑transferase subunit A/potassium transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,458,791 | 0 | A | T | 8.3% | 152.1 / 4.4 | 48 | intergenic (+39/‑229) | pcaJ/trkH‑I | 3‑oxoadipate CoA‑transferase subunit A/potassium transporter |
| Reads supporting (aligned to +/- strand): ref base A (20/24); new base T (2/2); total (22/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01 | |||||||||||
CCTGGCCGTACTGGAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAGCGGCGTAAAAAATTGCCG > NC_002947/4458644‑4458939 | ccTGGCCGTACTGGAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCAcaat > 2:1259062/1‑150 (MQ=255) cTGGCCGTACTGGAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCAcaatc > 2:395673/1‑150 (MQ=255) tGGCCGTACTGGAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCAcaatca > 2:521384/1‑150 (MQ=255) ggCCGTACTGGAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCAcaatcaa < 2:359860/150‑1 (MQ=255) ggCCGTACTGGAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCAcaatcaa < 2:692816/150‑1 (MQ=255) gCCGTACTGGAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCAcaatcaat < 1:1275770/150‑1 (MQ=255) gCCGTACTGGAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCAcaatcaat < 1:1276056/150‑1 (MQ=255) cGTACTGGAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAg > 1:1334153/1‑150 (MQ=255) cTGGAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGcc < 2:1334153/150‑1 (MQ=255) gAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGca < 1:1204326/150‑1 (MQ=255) aGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGcaca < 1:1259062/150‑1 (MQ=255) tGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCcgcg > 2:867922/1‑150 (MQ=255) aGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGc > 1:1356535/1‑150 (MQ=255) gCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAgg < 2:934725/150‑1 (MQ=255) gCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAgg < 1:160230/150‑1 (MQ=255) tGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGa < 1:108150/150‑1 (MQ=255) aaaGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGc > 1:17485/1‑150 (MQ=255) aGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACTATTGATTGTGCCGcaga > 2:501014/1‑127 (MQ=255) aGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACTATTGATTGTGCCGcaga < 1:501014/129‑3 (MQ=255) tCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACTATTGATTGTGCCGcaga > 2:949281/1‑122 (MQ=255) tCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACTATTGATTGTGCCGcaga < 1:949281/124‑3 (MQ=255) gAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGcgccgc > 1:1291018/1‑130 (MQ=255) gAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGcgccgc < 2:1291018/130‑1 (MQ=255) tCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTga < 1:395673/150‑1 (MQ=255) tCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTga < 2:914180/150‑1 (MQ=255) cTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTgag < 1:867922/150‑1 (MQ=255) tGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTgaga < 1:1086977/150‑1 (MQ=255) tGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTgaga < 2:1131354/150‑1 (MQ=255) aCGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTc < 1:521384/150‑1 (MQ=255) gCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAg > 2:1378275/1‑150 (MQ=255) cAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGa > 1:185006/1‑150 (MQ=255) aaaCTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATgg > 2:183762/1‑150 (MQ=255) aCTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTga > 1:536251/1‑116 (MQ=255) aCTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTga < 2:536251/116‑1 (MQ=255) aCTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGaa < 2:185006/150‑1 (MQ=255) tCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAAct > 1:906497/1‑150 (MQ=255) tGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTACATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCg < 2:870889/150‑1 (MQ=255) gTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGgaga < 1:1378275/150‑1 (MQ=255) cTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCaa > 1:382734/1‑150 (MQ=255) aTCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGt < 1:47153/91‑1 (MQ=255) aTCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGt > 2:47153/1‑91 (MQ=255) aaGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAggg < 1:487920/78‑1 (MQ=255) aaGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAggg > 2:487920/1‑78 (MQ=255) gTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGa < 2:252161/150‑1 (MQ=255) gTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGa < 2:382734/150‑1 (MQ=255) aGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCg > 2:842538/1‑150 (MQ=255) ggAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAgta > 2:973987/1‑63 (MQ=255) ggAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAgta < 1:973987/65‑3 (MQ=255) ttAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAg > 1:483993/1‑150 (MQ=255) aTGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAgcggc > 1:484189/1‑150 (MQ=255) aTGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAgcggc < 2:1234675/150‑1 (MQ=255) tctGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAGCGGCGTaaa > 2:1206818/1‑150 (MQ=255) tctGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAGCGGCGTaaa > 1:877714/1‑150 (MQ=255) ggCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGcc < 2:1367232/71‑1 (MQ=255) ggCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGcc > 1:1367232/1‑71 (MQ=255) cacaATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAgcg < 2:282742/136‑1 (MQ=255) cacaATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAgcg > 1:282742/1‑136 (MQ=255) caatcaatAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAGCGGCGTAAAAAATTGccc < 2:877714/150‑2 (MQ=255) aatcaatAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGa > 2:1322461/1‑128 (MQ=255) aatcaatAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGa < 1:1322461/128‑1 (MQ=255) | CCTGGCCGTACTGGAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAGCGGCGTAAAAAATTGCCG > NC_002947/4458644‑4458939 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |