Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,458,795 A→G 8.0% intergenic (+43/‑225) pcaJ → / → trkH‑I 3‑oxoadipate CoA‑transferase subunit A/potassium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,458,7950AG 8.0% 144.3 / 2.7 50intergenic (+43/‑225)pcaJ/trkH‑I3‑oxoadipate CoA‑transferase subunit A/potassium transporter
Reads supporting (aligned to +/- strand):  ref base A (21/25);  new base G (2/2);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

TGGCCGTACTGGAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAGCGGCGTAAAAAATTGCCG  >  NC_002947/4458646‑4458939
                                                                                                                                                     |                                                                                                                                                
tGGCCGTACTGGAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCAcaatca                                                                                                                                                  >  2:521384/1‑150 (MQ=255)
 ggCCGTACTGGAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCAcaatcaa                                                                                                                                                 <  2:359860/150‑1 (MQ=255)
 ggCCGTACTGGAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCAcaatcaa                                                                                                                                                 <  2:692816/150‑1 (MQ=255)
  gCCGTACTGGAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCAcaatcaat                                                                                                                                                <  1:1275770/150‑1 (MQ=255)
  gCCGTACTGGAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCAcaatcaat                                                                                                                                                <  1:1276056/150‑1 (MQ=255)
    cGTACTGGAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAg                                                                                                                                              >  1:1334153/1‑150 (MQ=255)
        cTGGAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGcc                                                                                                                                          <  2:1334153/150‑1 (MQ=255)
           gAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGca                                                                                                                                       <  1:1204326/150‑1 (MQ=255)
             aGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGcaca                                                                                                                                     <  1:1259062/150‑1 (MQ=255)
                      tGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCcgcg                                                                                                                            >  2:867922/1‑150 (MQ=255)
                         aGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGc                                                                                                                         >  1:1356535/1‑150 (MQ=255)
                            gCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAgg                                                                                                                      <  1:160230/150‑1 (MQ=255)
                            gCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAgg                                                                                                                      <  2:934725/150‑1 (MQ=255)
                              tGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGa                                                                                                                    <  1:108150/150‑1 (MQ=255)
                                aaaGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGc                                                                                                                  >  1:17485/1‑150 (MQ=255)
                                  aGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACTATTGATTGTGCCGcaga                                                                                                                                     >  2:501014/1‑127 (MQ=255)
                                  aGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACTATTGATTGTGCCGcaga                                                                                                                                     <  1:501014/129‑3 (MQ=255)
                                       tCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACTATTGATTGTGCCGcaga                                                                                                                                     >  2:949281/1‑122 (MQ=255)
                                       tCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACTATTGATTGTGCCGcaga                                                                                                                                     <  1:949281/124‑3 (MQ=255)
                                         gAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGcgccgc                                                                                                                             <  2:1291018/130‑1 (MQ=255)
                                         gAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGcgccgc                                                                                                                             >  1:1291018/1‑130 (MQ=255)
                                             tCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTga                                                                                                     <  2:914180/150‑1 (MQ=255)
                                             tCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTga                                                                                                     <  1:395673/150‑1 (MQ=255)
                                              cTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTgag                                                                                                    <  1:867922/150‑1 (MQ=255)
                                               tGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTgaga                                                                                                   <  1:1086977/150‑1 (MQ=255)
                                               tGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTgaga                                                                                                   <  2:1131354/150‑1 (MQ=255)
                                                                  aCGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTc                                                                                <  1:521384/150‑1 (MQ=255)
                                                                      gCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAg                                                                            >  2:1378275/1‑150 (MQ=255)
                                                                          cAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGa                                                                        >  1:185006/1‑150 (MQ=255)
                                                                             aaaCTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATgg                                                                     >  2:183762/1‑150 (MQ=255)
                                                                               aCTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTga                                                                                                     >  1:536251/1‑116 (MQ=255)
                                                                               aCTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTga                                                                                                     <  2:536251/116‑1 (MQ=255)
                                                                               aCTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGaa                                                                   <  2:185006/150‑1 (MQ=255)
                                                                                 tCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAAct                                                                 >  1:906497/1‑150 (MQ=255)
                                                                                     tGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTACATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCg                                                             <  2:870889/150‑1 (MQ=255)
                                                                                         gTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGgaga                                                         <  1:1378275/150‑1 (MQ=255)
                                                                                               cTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCaa                                                   >  1:382734/1‑150 (MQ=255)
                                                                                                  aTCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGt                                                                                                           <  1:47153/91‑1 (MQ=255)
                                                                                                  aTCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGt                                                                                                           >  2:47153/1‑91 (MQ=255)
                                                                                                     aaGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAggg                                                                                                                     <  1:487920/78‑1 (MQ=255)
                                                                                                     aaGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAggg                                                                                                                     >  2:487920/1‑78 (MQ=255)
                                                                                                       gTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGa                                           <  2:252161/150‑1 (MQ=255)
                                                                                                       gTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGa                                           <  2:382734/150‑1 (MQ=255)
                                                                                                              aGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCg                                    >  2:842538/1‑150 (MQ=255)
                                                                                                               ggAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAgta                                                                                                                        >  2:973987/1‑63 (MQ=255)
                                                                                                               ggAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAgta                                                                                                                        <  1:973987/65‑3 (MQ=255)
                                                                                                                      ttAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAg                            >  1:483993/1‑150 (MQ=255)
                                                                                                                                  aTGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAgcggc                <  2:1234675/150‑1 (MQ=255)
                                                                                                                                  aTGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAgcggc                >  1:484189/1‑150 (MQ=255)
                                                                                                                                       tctGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAGCGGCGTaaa           >  2:1206818/1‑150 (MQ=255)
                                                                                                                                       tctGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAGCGGCGTaaa           >  1:877714/1‑150 (MQ=255)
                                                                                                                                            ggCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGcc                                                                                     <  2:1367232/71‑1 (MQ=255)
                                                                                                                                            ggCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGcc                                                                                     >  1:1367232/1‑71 (MQ=255)
                                                                                                                                              cacaATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAgcg                  <  2:282742/136‑1 (MQ=255)
                                                                                                                                              cacaATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAgcg                  >  1:282742/1‑136 (MQ=255)
                                                                                                                                                caatcaatAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAGCGGCGTAAAAAATTGccc  <  2:877714/150‑2 (MQ=255)
                                                                                                                                                 aatcaatAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGa                       >  2:1322461/1‑128 (MQ=255)
                                                                                                                                                 aatcaatAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGa                       <  1:1322461/128‑1 (MQ=255)
                                                                                                                                                   tcaatAGTGCCGCACAAGCGCCGCGAGCAGGGAGCAcgc                                                                                                              >  2:247983/1‑39 (MQ=255)
                                                                                                                                                   tcaatAGTGCCGCACAAGCGCCGCGAGCAGGGAGCAcgc                                                                                                              <  1:247983/39‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
TGGCCGTACTGGAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAGCGGCGTAAAAAATTGCCG  >  NC_002947/4458646‑4458939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑