Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,458,798 A→T 7.2% intergenic (+46/‑222) pcaJ → / → trkH‑I 3‑oxoadipate CoA‑transferase subunit A/potassium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,458,7980AT 7.2% 168.4 / 2.9 55intergenic (+46/‑222)pcaJ/trkH‑I3‑oxoadipate CoA‑transferase subunit A/potassium transporter
Reads supporting (aligned to +/- strand):  ref base A (23/28);  new base T (2/2);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

CGTACTGGAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAGCGGCGTAAAAAATTGCCG  >  NC_002947/4458650‑4458939
                                                                                                                                                    |                                                                                                                                             
cGTACTGGAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAg                                                                                                                                              >  1:1334153/1‑150 (MQ=255)
    cTGGAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGcc                                                                                                                                          <  2:1334153/150‑1 (MQ=255)
       gAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGca                                                                                                                                       <  1:1204326/150‑1 (MQ=255)
         aGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGcaca                                                                                                                                     <  1:1259062/150‑1 (MQ=255)
                  tGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCcgcg                                                                                                                            >  2:867922/1‑150 (MQ=255)
                     aGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGc                                                                                                                         >  1:1356535/1‑150 (MQ=255)
                        gCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAgg                                                                                                                      <  1:160230/150‑1 (MQ=255)
                        gCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAgg                                                                                                                      <  2:934725/150‑1 (MQ=255)
                          tGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGa                                                                                                                    <  1:108150/150‑1 (MQ=255)
                            aaaGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGc                                                                                                                  >  1:17485/1‑150 (MQ=255)
                              aGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACTATTGATTGTGCCGcaga                                                                                                                                     >  2:501014/1‑127 (MQ=255)
                              aGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACTATTGATTGTGCCGcaga                                                                                                                                     <  1:501014/129‑3 (MQ=255)
                                   tCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACTATTGATTGTGCCGcaga                                                                                                                                     >  2:949281/1‑122 (MQ=255)
                                   tCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACTATTGATTGTGCCGcaga                                                                                                                                     <  1:949281/124‑3 (MQ=255)
                                     gAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGcgccgc                                                                                                                             >  1:1291018/1‑130 (MQ=255)
                                     gAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGcgccgc                                                                                                                             <  2:1291018/130‑1 (MQ=255)
                                         tCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTga                                                                                                     <  2:914180/150‑1 (MQ=255)
                                         tCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTga                                                                                                     <  1:395673/150‑1 (MQ=255)
                                          cTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTgag                                                                                                    <  1:867922/150‑1 (MQ=255)
                                           tGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTgaga                                                                                                   <  1:1086977/150‑1 (MQ=255)
                                           tGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTgaga                                                                                                   <  2:1131354/150‑1 (MQ=255)
                                                              aCGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTc                                                                                <  1:521384/150‑1 (MQ=255)
                                                                  gCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAg                                                                            >  2:1378275/1‑150 (MQ=255)
                                                                      cAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGa                                                                        >  1:185006/1‑150 (MQ=255)
                                                                         aaaCTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATgg                                                                     >  2:183762/1‑150 (MQ=255)
                                                                           aCTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTga                                                                                                     >  1:536251/1‑116 (MQ=255)
                                                                           aCTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTga                                                                                                     <  2:536251/116‑1 (MQ=255)
                                                                           aCTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGaa                                                                   <  2:185006/150‑1 (MQ=255)
                                                                             tCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAAct                                                                 >  1:906497/1‑150 (MQ=255)
                                                                                 tGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTACATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCg                                                             <  2:870889/150‑1 (MQ=255)
                                                                                     gTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGgaga                                                         <  1:1378275/150‑1 (MQ=255)
                                                                                           cTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCaa                                                   >  1:382734/1‑150 (MQ=255)
                                                                                              aTCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGt                                                                                                           <  1:47153/91‑1 (MQ=255)
                                                                                              aTCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGt                                                                                                           >  2:47153/1‑91 (MQ=255)
                                                                                                 aaGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAggg                                                                                                                     >  2:487920/1‑78 (MQ=255)
                                                                                                 aaGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAggg                                                                                                                     <  1:487920/78‑1 (MQ=255)
                                                                                                   gTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGa                                           <  2:382734/150‑1 (MQ=255)
                                                                                                   gTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGa                                           <  2:252161/150‑1 (MQ=255)
                                                                                                          aGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCg                                    >  2:842538/1‑150 (MQ=255)
                                                                                                           ggAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAgta                                                                                                                        >  2:973987/1‑63 (MQ=255)
                                                                                                           ggAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAgta                                                                                                                        <  1:973987/65‑3 (MQ=255)
                                                                                                                  ttAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAg                            >  1:483993/1‑150 (MQ=255)
                                                                                                                              aTGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAgcggc                >  1:484189/1‑150 (MQ=255)
                                                                                                                              aTGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAgcggc                <  2:1234675/150‑1 (MQ=255)
                                                                                                                                   tctGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAGCGGCGTaaa           >  1:877714/1‑150 (MQ=255)
                                                                                                                                   tctGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAGCGGCGTaaa           >  2:1206818/1‑150 (MQ=255)
                                                                                                                                        ggCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGcc                                                                                     <  2:1367232/71‑1 (MQ=255)
                                                                                                                                        ggCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGcc                                                                                     >  1:1367232/1‑71 (MQ=255)
                                                                                                                                          cacaATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAgcg                  <  2:282742/136‑1 (MQ=255)
                                                                                                                                          cacaATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAgcg                  >  1:282742/1‑136 (MQ=255)
                                                                                                                                            caatcaatAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAGCGGCGTAAAAAATTGccc  <  2:877714/150‑2 (MQ=255)
                                                                                                                                             aatcaatAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGa                       >  2:1322461/1‑128 (MQ=255)
                                                                                                                                             aatcaatAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGa                       <  1:1322461/128‑1 (MQ=255)
                                                                                                                                               tcaatAGTGCCGCACAAGCGCCGCGAGCAGGGAGCAcgc                                                                                                              >  2:247983/1‑39 (MQ=255)
                                                                                                                                               tcaatAGTGCCGCACAAGCGCCGCGAGCAGGGAGCAcgc                                                                                                              <  1:247983/39‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
CGTACTGGAAGTGACACCTGAAGGGCTGAAAGTGGTCGAAATCTGCGCGGACATCGACTTTGACGAGCTGCAGAAACTCAGTGGCGTGCCGCTGATCAAGTGATCCAGGAAGACTTAGGGCTTTCCATGCCTCTGCGGCACAATCAATAGTGCCGCACAAGCGCCGCGAGCAGGGAGCACGCGGTGCTTGAGACTACCTTTACAGCCGCTTCGAAGCCGATGGAACTCTCGGAGAGGTCAACCCAATGAGCTATCGAGCGGGAGCCAGATAGCGGCGTAAAAAATTGCCG  >  NC_002947/4458650‑4458939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑