Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,478,916 A→T 10.0% T75S (ACC→TCC)  ybdR → Zn‑dependent oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,478,9160AT10.0% 108.0 / 2.8 39T75S (ACC→TCC) ybdRZn‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (16/18);  new base T (2/2);  total (18/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

CAGACGACATCATCTTAAGGGTGACGGCCACCGCCATCTGCGGATCCGACCTCCACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAACCACCAATACCGGGCGCGGATCGATCATCAACAAAAAGGGCATACCACCAGGCGC  >  NC_002947/4478767‑4479064
                                                                                                                                                     |                                                                                                                                                    
cAGACGACATCATCTTAAGGGTGACGGCCACCGCCATCTGCGGATCCGACCTCCACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTgacga                                                                                                                                                      <  1:1025584/150‑1 (MQ=255)
 aGACGACATCATCGTAAGGGTGACGGCCACCGCCATCTGCGGATCCGACCTCCACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCAACGTGGAAGAGGTTGGCAGGGACGTgacgac                                                                                                                                                     >  1:1212817/1‑150 (MQ=255)
        atcatcTTAAGGGTGACGGCCACCGCCATCTGCGGATCCGACCTCCACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAg                                                                                                                                              <  1:266947/150‑1 (MQ=255)
        atcatcTTAAGGGTGACGGCCACCGCCATCTGCGGATCCGACCTCCACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAg                                                                                                                                              >  2:706003/1‑150 (MQ=255)
              ttAAGGGTGACGGCCACCGCCATCTGCGGATCCGACCTCCACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGc                                                                                                                                        <  1:1104731/150‑1 (MQ=255)
                  gggTGACGGCCACCGCCATCTGCGGATCCGACCTCCACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGAcc                                                                                                                                    <  1:291848/150‑1 (MQ=255)
                         ggCCACCGCCATCTGCGGATCCGACCTCCACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAgtggt                                                                                                                             <  1:30527/150‑1 (MQ=255)
                               cGCCATCTGCGGATCCGACCTCCACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCATGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGaagat                                                                                                                       >  1:1223705/1‑146 (MQ=255)
                                              cGACCTCCACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGGCGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTtg                                                                                                        >  2:223934/1‑150 (MQ=255)
                                                      aCCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGTCGTCACGTCCCt                                                                                                                                                  >  1:975573/1‑100 (MQ=255)
                                                      aCCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGTCGTCACGTCCCt                                                                                                                                                  <  2:975573/100‑1 (MQ=255)
                                                      aCCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGtt                                                                                                >  1:1145065/1‑150 (MQ=255)
                                                      aCCTCTAGCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGCCGTGACGTTCCTGCAGGTTGGCGACCGATTGGTGATTCCTTTTGTCATCGCTTGTGGAAGtt                                                                                                <  1:175441/150‑1 (MQ=255)
                                                             tCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCttttt                                                                                         >  1:45078/1‑150 (MQ=255)
                                                             tCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCttttt                                                                                         >  1:882376/1‑150 (MQ=255)
                                                                    aaGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAgtg                                                                                                                               <  2:1360284/105‑1 (MQ=255)
                                                                    aaGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAgtg                                                                                                                               >  1:1360284/1‑105 (MQ=255)
                                                                    aaGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGAATACGGTGCAGGTTTGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCcag                                                                                  >  1:738331/1‑150 (MQ=255)
                                                                         cccGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAgtgg                                                                                                                              >  1:43495/1‑101 (MQ=255)
                                                                         cccGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAgtgg                                                                                                                              <  2:43495/101‑1 (MQ=255)
                                                                          ccGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGCCACTGCAGGTTGGTGGCCGAGCGGTTAGTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGAt                                                                            <  1:885507/150‑1 (MQ=255)
                                                                                       aaGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCt                                                               <  1:560874/150‑1 (MQ=255)
                                                                                                 cTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAaccac                                                     <  2:859301/150‑1 (MQ=255)
                                                                                                       cctcGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAACCACCAATAc                                               >  1:1426565/4‑150 (MQ=255)
                                                                                                         aCGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTc                                                                                        <  1:209644/107‑1 (MQ=255)
                                                                                                         aCGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTc                                                                                        >  2:209644/1‑107 (MQ=255)
                                                                                                          cGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAACCACCAATACCgg                                            <  2:1426565/150‑1 (MQ=255)
                                                                                                                          gTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAACCACCAATACCGGGCGCGGATCGatcatc                            <  2:878931/150‑1 (MQ=255)
                                                                                                                                 caacctgCAGGGTCGTCACGTCCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAACCACCAATACCGGGCGCGGATCGATCATCAACaaaa                     >  2:884699/7‑150 (MQ=255)
                                                                                                                                    ttGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAaccac                                                     <  1:1102528/115‑1 (MQ=255)
                                                                                                                                    ttGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAaccac                                                     >  2:1102528/1‑115 (MQ=255)
                                                                                                                                     tGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAACCACCAATACCGGGCGCGGATCGATCATCAACAAAAAggg                 <  1:706003/150‑1 (MQ=255)
                                                                                                                                        cAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAACCACCAATACCGGGCGCGGATCGATCATCAACAAAAAGGGCAt              <  2:738331/150‑1 (MQ=255)
                                                                                                                                         aGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAac                                                        >  2:687266/1‑107 (MQ=255)
                                                                                                                                         aGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAac                                                        <  1:687266/107‑1 (MQ=255)
                                                                                                                                          gggACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAACCACCAATACCGGGCGCGGATCGATCATCAACAAAAAGGGCATac            >  1:640813/1‑150 (MQ=255)
                                                                                                                                             aCGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAACCACCAATACCGGGCGCGGATCGATCATCAACAAAAAGGGCAt              <  2:187234/145‑1 (MQ=255)
                                                                                                                                             aCGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAACCACCAATACCGGGCGCGGATCGATCATCAACAAAAAGGGCAt              >  1:187234/1‑145 (MQ=255)
                                                                                                                                               gTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAACCACCAATACCGGgcgc                                        <  1:746342/117‑1 (MQ=255)
                                                                                                                                               gTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAACCACCAATACCGGgcgc                                        >  2:746342/1‑117 (MQ=255)
                                                                                                                                                tGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAACCACCAATACCGGGCGCGGATCGATCATCAACAAAAAGGGCATACCACCAg      <  2:45078/150‑1 (MQ=255)
                                                                                                                                                    gacCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAACCACCAATACCGGGCGCGGATCGATCATCAACAAAAAGGGCATACCACCAGgcgc  >  2:141830/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CAGACGACATCATCTTAAGGGTGACGGCCACCGCCATCTGCGGATCCGACCTCCACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAACCACCAATACCGGGCGCGGATCGATCATCAACAAAAAGGGCATACCACCAGGCGC  >  NC_002947/4478767‑4479064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑