Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,484,771 A→T 10.5% intergenic (+371/‑98) PP_3976 → / → PP_3977 isochorismatase family hydrolase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,484,7710AT10.5% 108.4 / 4.9 38intergenic (+371/‑98)PP_3976/PP_3977isochorismatase family hydrolase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (16/18);  new base T (2/2);  total (18/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01

CTCAAACCCTGGCAGGTCGCAACGGCCAAGCAGATGATGTTGGAGGCATATCGGTCACAGCATGCGGAAATGAACAGAGAAATGCGGGCGTGATCCGTATGACAAGGGTAGAAAATCGGAGCCCCAAAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCAAACCCTTATCCTAGGCTCCTACCTACAGACTTAATTTCTGATCGGTAGCTCGACGTCCTTTATGGACTGTAAGCTAGCAAAACCGGGTCTTCCGCGCAAGCTTCAG  >  NC_002947/4484623‑4484913
                                                                                                                                                    |                                                                                                                                              
cTCAAACCCTGGCAGGTCGCAACGGCCAAGCAGATGATGTTGGAGGCATATCGGTCACAGCATGCGGAAATGAACAGAGAAATGCGGGCGTGATCCGTATGACAAGGGTAGAAAATCGGAGCCCCAAAAACGCAAAAAGCCCTGaataat                                                                                                                                               >  1:912727/1‑150 (MQ=255)
 tCAAACCCTGGCAGGTCGCAACGGCCAAGCAGATGATGTTGGAGGCATATCGGTCACAGCATGCGGAAATGAACAGAGAAATGCGGGCGTGATCCGTATGACAAGGGTAGAAAATCGGAGCCCCAAAAACGCAAAAAGCCCTGAATAATc                                                                                                                                              >  1:1017978/1‑150 (MQ=255)
   aaaCCCTGGCAGGTCGCAACGGCCAAGCAGATGATGTTGGAGGCATATCGGTCACAGCATGCGGAAATGAACAGAGAAATGCGGGCGTGATCCGTATGACAAGGGTAGAAAATCGGAGCCCCAAAAACGCAAAAAGCCCTGAATAATCAg                                                                                                                                            <  1:1039040/150‑1 (MQ=255)
     aCCCTGGCAGGTCGCAACGGCCAAGCAGATGATGTTGGAGGCATATCGGTCACAGCATGCGGAAATGAACAGAGAAATGCGGGCGTGATCCGTATGACAAGGGTAGAAAATCGGAGCCCCAAAAACGCAAAAAGCCCTGAATAATCAggg                                                                                                                                          >  1:693364/1‑150 (MQ=255)
            cAGGTCGCAACGGCCAAGCAGATGATGTTGGAGGCATATCGGTCACAGCATGCGGAAATGAACAGAGAAATGCGGGCGTGATCCGTATGACAAGGGTAGAAAATCGGAGCCCCAAAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGaa                                                                                                                                   <  2:912727/150‑1 (MQ=255)
                tCGCAACGGCCAAGCAGATGATGTTGGAGGCATATCGGTCACAGCATGCGGAAATGAACAGAGAAATGCGGGCGTGATCCGTATGACAAGGGTAGAAAATCGGAGCCCCAAAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATg                                                                                                                               >  1:53673/1‑150 (MQ=255)
                tCGCAACGGCCAAGCAGATGATGTTGGAGGCATATCGGTCACAGCATGCGGAAATGAACAGAGAAATGCGGGCGTGATCCGTATGACAAGGGTAGAAAATCGGAGCCCCAAAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATg                                                                                                                               <  1:765923/150‑1 (MQ=255)
                 cGCAACGGCCAAGCAGATGATGTTGGAGGCATATCGGTCACAGCATGCGGAAATGAACAGAGAAATGCGGGCGTGATCCGTATGACAAGGGTAGAAAATCGGAGCCCCAAAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATgg                                                                                                                              <  2:1017978/150‑1 (MQ=255)
                         ccAAGCAGATGATGTTGGAGGCATATCGGTCACAGCATGCGGAAATGAACAGAGAAATGCGGGCGTGATCCGTATGACAAGGGTAGAAAATCGGAGCCCCAAAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCg                                                                                                                            <  2:766848/144‑1 (MQ=255)
                         ccAAGCAGATGATGTTGGAGGCATATCGGTCACAGCATGCGGAAATGAACAGAGAAATGCGGGCGTGATCCGTATGACAAGGGTAGAAAATCGGAGCCCCAAAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCg                                                                                                                            >  1:766848/1‑144 (MQ=255)
                           aaGCAGATGATGTCGGAGGCATATCGGTCACAGCATGCGGAAATGAACAGAGAGATGCGGGCGTGATCCGTATGACAAGGGTAGAAAATCGGAGCCCCAAAAACGCAAATAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTa                                                                                                                    <  1:1360292/150‑1 (MQ=255)
                            aGCAGATGATGTTGGAGGCATATGGGTCACAGCATGCGGAAATGAACAGAGAAATGCGGGCGTGATCCGTATGACAAGGGTAGAAAATCGGAGCCCCAAAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTag                                                                                                                   <  1:594188/150‑1 (MQ=255)
                                gatgatGTTGGAGGCATATCGGTCACAGCATGCGGAAATGAACAGAGAAATGCGGGCGTGATCCGTATGACAAGGGTAGAAAATCGGAGCCCCAAAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTaga                                                                                                                  <  2:69809/147‑1 (MQ=255)
                                gatgatGTTGGAGGCATATCGGTCACAGCATGCGGAAATGAACAGAGAAATGCGGGCGTGATCCGTATGACAAGGGTAGAAAATCGGAGCCCCAAAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTaga                                                                                                                  >  1:69809/1‑147 (MQ=255)
                                        tGGAGGCATATCGGTCACAGCATGCGGAAATGAACAGAGAAATGCGGGCGTGATCCGTATGACAAGGGTAGAAAATCGGAGCCCCAAAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCAAAccc                                                                                                       <  1:60083/150‑1 (MQ=255)
                                              cATATCGGTCACAGCATGCGGAAATGAACAGAGAAATGCGGGCGTGATCCGTATGACAAGGGTAGAAAATCGGAGCCCCAAAAACGCAAAAAGCCCTGAATAATCaa                                                                                                                                            <  2:877904/107‑2 (MQ=255)
                                              cATATCGGTCACAGCATGCGGAAATGAACAGAGAAATGCGGGCGTGATCCGTATGACAAGGGTAGAAAATCGGAGCCCCAAAAACGCAAAAAGCCCTGAATAATCAg                                                                                                                                            >  1:877904/1‑107 (MQ=255)
                                                tatCGGTCACAGCATGCGGAAATGAACAGAGAAATGCGGGCGTGATCCGTATGACAAGGGTAGAAAATCGGAGCCCCACCTGTGAGAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGCAGAACCTTAAACCCTTATCCTa                                                                                               >  1:1156555/1‑150 (MQ=37)
                                                  tCGGTCACAGCATGCGGAAATGAACAGAGAAATGCGGGCGTGATCCGTATGACAAGGGTAGAAAATCGGAGCCCCAAAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCAAACCCTTATCCTAgg                                                                                             <  1:1112686/150‑1 (MQ=255)
                                                                       gAACAGAGAAATGCGGGCGTGATCCGTATGACAAGGGTAGAAAATCGGAGCCCCAAAAACGCAAAAAGCCCTGATTATTCAGGGCttttt                                                                                                                                    <  1:496503/90‑3 (MQ=39)
                                                                       gAACAGAGAAATGCGGGCGTGATCCGTATGACAAGGGTAGAAAATCGGAGCCCCAAAAACGCAAAAAGCCCTGATTATTCAGGGCttttt                                                                                                                                    >  2:496503/1‑88 (MQ=39)
                                                                           agagAAATGCGGGCGTGATCCGTATGACAAGGGTAGAAAATCGGAGCCCCAAAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCAAACCCTTATCCTAGGCTCCTACCTACAGACTTAATTTCTg                                                                    >  1:514254/1‑150 (MQ=255)
                                                                            gagaAATGCGGGCGTGATCCGTATGACAAGGGTAGAAAATCGGAGCCCCAAAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCAAACCCTTATCCTAGGCTCCTACCTACAGACTTAATTTCTGa                                                                   <  2:693364/150‑1 (MQ=255)
                                                                             agaAATGCGGGCGTGATCCGTATGACAAGGGTAGAAAATCGGAGCCCCAAAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCAAACCCTTATCCTAGGCTCCTACCTACAGACTTAATTTCTGAt                                                                  <  1:757356/150‑1 (MQ=255)
                                                                                   gCGGGCGTGATCCGTATGACAAGGGTAGAAAATCGGAGCCCCAAAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGCTTCAAACCCTTATCCTAGGCTCCTACCTACAGACTTAATTCCTGATCGGTAg                                                            <  2:53673/150‑1 (MQ=255)
                                                                                                    gACAAGGGTAGAAAATCGGAGCCCCAAAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCAAACCCTTATCCTAGGCTCCTACCTACAGACTTAATTTCTGATCGGTAGCTCGACGTCCTTTATgg                                           >  2:552817/1‑150 (MQ=255)
                                                                                                                 aaTCGGAGCCCCAAAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATTGCGGAAGCGTAGAGATTCAAACCCTTATCCTAGGCTCCTACCTACAGACTTAATTTCTGATCGGTAGCTCGACGTCCTTTATGGACTGTAAGCTAGc                              >  1:408286/1‑150 (MQ=255)
                                                                                                                         ccccAAAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCAAACCCTTATCCTAGGCTCCTACCTACAGACTTAATTTCTGATCGGTAGCTCGACGTCCTTTATGGACTGTAAGCTAGCAAAACCgg                      <  1:552817/150‑1 (MQ=255)
                                                                                                                         ccccAAAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCAAACCCTTATCCTAGGCTCCTACCTACAGACTTAATTTCTGATCGGTAGCTCGACGTCCTTTATGGACTGTAAGCTAGCAAAACCgg                      >  2:327992/1‑150 (MQ=255)
                                                                                                                         ccccAAAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCAAACCCTTATCCTAGGCTCCTACCTACAGACTTAATTTCTGATCGGTAGCTCGACGTCCTTTATGGACTGTAAGCTAGCAAAACCgg                      <  2:521813/150‑1 (MQ=255)
                                                                                                                                 aCGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCAAACCCTTATCCTAGGCTCCTACCTACAGACTTAATTTCTGATCGGTAGCTCGACGTCCTTTATg                                            >  1:619740/1‑120 (MQ=255)
                                                                                                                                 aCGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCAAACCCTTATCCTAGGCTCCTACCTACAGACTTAATTTCTGATCGGTAGCTCGACGTCCTTTATg                                            <  2:619740/120‑1 (MQ=255)
                                                                                                                                   gCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCAAACCCTTATCCTAGGCTCCTACCTACAGACTTAATTTCTGATCGGTAGCTCGACGTCCTTTATGGACTGTAAGCTAGCAAAACCGGGTCTTCcgcg            >  1:936470/1‑150 (MQ=255)
                                                                                                                                    ttcaaaGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCAAACCCTTATCCTAGGCTCCTACCTACAGa                                                                               >  2:1401656/4‑82 (MQ=255)
                                                                                                                                    ttcaaaGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCAAACCCTTATCCTAGGCTCCTACCTACAGa                                                                               <  1:1401656/79‑1 (MQ=255)
                                                                                                                                        aaGCCCTGATTATTCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCAAACCCTTATCCTAGGCTCCTACCTACAGACTTAATTTCTGATCGGTAGCTCGACGTCCTTTATGGa                                          <  1:331704/115‑1 (MQ=255)
                                                                                                                                        aaGCCCTGATTATTCAGGGCTTTGAATATGGCGGAAGCGCAGAGATTCAAACCCTTATCCTAGGCTCCTACCTACAGACTTAATTTCTGATCGGTAGCTCGACGTCCTTTATGGa                                          >  2:331704/1‑115 (MQ=255)
                                                                                                                                        aaGCCCTGAATAATCAGGGCTTTGAATATGGAGGAAGCGTAGAGATTCAAACCCTTATCCTAGGCCCCTACCTACAGACTTAATTTCTGATCGGTAGCTCGACATCCTTTATGGACTGTAAGCTAGCAAAACCAGGTCTTCCACGCaatc       >  2:483876/1‑148 (MQ=38)
                                                                                                                                             cTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCAAACCCTTATCCTAGGCTCCTACCTACAGACTTAATTTCTGATCGGTAGCTCGACGTCCTTTATGGACTGTAAGCTAGCAAAACCGGGTCTTCCGCGCAAGCTTCAg  >  2:814777/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
CTCAAACCCTGGCAGGTCGCAACGGCCAAGCAGATGATGTTGGAGGCATATCGGTCACAGCATGCGGAAATGAACAGAGAAATGCGGGCGTGATCCGTATGACAAGGGTAGAAAATCGGAGCCCCAAAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCAAACCCTTATCCTAGGCTCCTACCTACAGACTTAATTTCTGATCGGTAGCTCGACGTCCTTTATGGACTGTAAGCTAGCAAAACCGGGTCTTCCGCGCAAGCTTCAG  >  NC_002947/4484623‑4484913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑