Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,512,506 C→A 10.9% G9V (GGT→GTT)  lolA ← outer membrane lipoprotein carrier protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,512,5060CA10.9% 127.7 / 5.7 46G9V (GGT→GTT) lolAouter membrane lipoprotein carrier protein
Reads supporting (aligned to +/- strand):  ref base C (21/20);  new base A (3/2);  total (24/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.26e-01

TCTGCGACTTTTCCAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGATCTTCAG  >  NC_002947/4512357‑4512653
                                                                                                                                                     |                                                                                                                                                   
tCTGCGACTTTTCCAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCCGGGCAGAAACCAACCGCATGCGAATCGCGCGCATGCGAATCCTCATTGAGCGTTTAAAAGGCCTGGCGCCAc                                                                                                                                                     <  2:375165/150‑1 (MQ=255)
 cTGCGACTTTTCCAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGTTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCAcc                                                                                                                                                    <  1:31140/150‑1 (MQ=255)
           tgcAGCAACGGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGTCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCca                                                                                                                                          <  2:790617/148‑1 (MQ=255)
            ccAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCcag                                                                                                                                         <  1:587617/150‑1 (MQ=255)
            ccAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCAACGGTGGCGCcag                                                                                                                                         <  1:1216038/150‑1 (MQ=255)
             cAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACGAACAGCATGCGAACCGCAGGCACGCCTATCAACATAGTGCGTTGAAAAGGCCTGGCGCCACCGTTGGTGCcagg                                                                                                                                        >  1:319535/1‑150 (MQ=255)
             cAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCAACGGTGGCGCcagg                                                                                                                                        <  2:324756/150‑1 (MQ=255)
                 aaCTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCcaggcagg                                                                                                                                    <  1:263270/150‑1 (MQ=255)
                   cTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCCCCGTTGGCGCCAGGCAGGgt                                                                                                                                  >  2:264923/1‑150 (MQ=255)
                   cTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGgt                                                                                                                                  >  2:807345/1‑150 (MQ=255)
                         cAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTtcat                                                                                                                            <  1:877659/150‑1 (MQ=255)
                              gTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAAAAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTc                                                                                                                       <  2:404173/150‑1 (MQ=255)
                                  tACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTcgcgc                                                                                                                   >  2:1385528/1‑150 (MQ=255)
                                             cACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCgg                                                                                                        <  2:857576/150‑1 (MQ=255)
                                                      aaGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCAc                                                                                               <  1:334940/150‑1 (MQ=255)
                                                          aGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTcc                                                                                           >  2:1337432/1‑150 (MQ=255)
                                                          aGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTcc                                                                                           >  1:829343/1‑150 (MQ=255)
                                                                               ggCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATc                                                                      <  2:1419595/150‑1 (MQ=255)
                                                                                  aGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCggg                                                                   <  2:559504/150‑1 (MQ=255)
                                                                                    aaaCCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGt                                                                 <  1:264923/150‑1 (MQ=255)
                                                                                     aaCCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTg                                                                >  2:1307029/1‑150 (MQ=255)
                                                                                              cATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCg                                                       <  2:829343/150‑1 (MQ=255)
                                                                                               aTGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAg                                                                                     <  2:1008415/119‑1 (MQ=255)
                                                                                               aTGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAg                                                                                     >  1:1008415/1‑119 (MQ=255)
                                                                                                            gcATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGa                                         >  2:1031393/1‑150 (MQ=255)
                                                                                                              aTGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACt                                       >  1:936906/1‑150 (MQ=255)
                                                                                                                  gAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTGCGAGGTGGTGACAACGCCGGCCATCTCCATCGACTCGAt                                   >  1:74146/1‑150 (MQ=255)
                                                                                                                   aaTCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGTTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATc                                           <  2:975056/141‑1 (MQ=255)
                                                                                                                   aaTCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGTTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATc                                           >  1:975056/1‑141 (MQ=255)
                                                                                                                      ccTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATg                               >  1:1098989/1‑150 (MQ=255)
                                                                                                                       cTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGTGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATgc                              <  2:319535/150‑1 (MQ=255)
                                                                                                                       cTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGGGACAACGCCGGCCATCTCCATCGACTCGATCATgc                              >  1:1148843/1‑150 (MQ=255)
                                                                                                                         cATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATgcgc                            <  1:1337432/150‑1 (MQ=255)
                                                                                                                          aTTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATgcgcg                           >  2:640192/1‑150 (MQ=255)
                                                                                                                           ttGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATgcgcgc                          <  1:1307029/150‑1 (MQ=255)
                                                                                                                                    gAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGATGAATGCCCGCATGTGCCGCCCGGGGCTATCAATTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGtt                 <  1:1385528/150‑1 (MQ=255)
                                                                                                                                     aaaaGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTg                >  1:1292173/1‑150 (MQ=255)
                                                                                                                                       cctgCCTGGCGCCAACGGTGGCGCCAGGCCGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTa              >  1:549710/4‑150 (MQ=255)
                                                                                                                                        ctgCCTGGCGCCAACGGTGGCGCCAGGCCGGGTGTTCATCAGTCGCGAGGTCCGTCCGGGGGTATCACTTCCAGCGAGCCGTTGCTGATAATCGCGTGGAGAGCACCGGCCATCTCCATCGACTCGATCTTGCGCGCGGCACGGTTGttg             >  2:691665/3‑148 (MQ=255)
                                                                                                                                            cTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGa         >  2:1269460/1‑150 (MQ=255)
                                                                                                                                             tGGCGCCAACGGTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGAt        >  1:887750/1‑150 (MQ=255)
                                                                                                                                              ggCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGATc       <  1:807345/150‑1 (MQ=255)
                                                                                                                                                 gcCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGATCTTc    <  2:282463/150‑1 (MQ=255)
                                                                                                                                                 gcCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGATCTTc    >  1:1043348/1‑150 (MQ=255)
                                                                                                                                                  ccACAGTTGGCGCCCGGAAGGGTGTTCATGCGTAGCTAGAGCAGCCTGAAGCTATCACTACCGGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGATCTTCa   <  2:74146/150‑1 (MQ=255)
                                                                                                                                                   cACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGATCTTCAg  >  1:71383/1‑150 (MQ=255)
                                                                                                                                                   cACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGAGCTTCAg  >  1:524174/1‑150 (MQ=255)
                                                                                                                                                   cACCGTTGGCGCCAGACAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGATCTTCAg  >  1:926813/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TCTGCGACTTTTCCAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGATCTTCAG  >  NC_002947/4512357‑4512653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑