Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,512,509	T→G	10.6%	N8T (AAC→ACC)	lolA ←	outer membrane lipoprotein carrier protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,512,509	0	T	G	10.6%	116.8 / 7.3	47	N8T (AAC→ACC)	lolA	outer membrane lipoprotein carrier protein
Reads supporting (aligned to +/- strand):  ref base T (21/21);  new base G (3/2);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01

TCCAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGATCTTCAGCTT  >  NC_002947/4512368‑4512656                                                                                                                                              |                                                                                                                                                    tgcAGCAACGGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGTCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCca                                                                                                                                             <  2:790617/148‑1 (MQ=255)  ccAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCcag                                                                                                                                            <  1:587617/150‑1 (MQ=255)  ccAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCAACGGTGGCGCcag                                                                                                                                            <  1:1216038/150‑1 (MQ=255)   cAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACGAACAGCATGCGAACCGCAGGCACGCCTATCAACATAGTGCGTTGAAAAGGCCTGGCGCCACCGTTGGTGCcagg                                                                                                                                           >  1:319535/1‑150 (MQ=255)   cAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCAACGGTGGCGCcagg                                                                                                                                           <  2:324756/150‑1 (MQ=255)       aaCTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCcaggcagg                                                                                                                                       <  1:263270/150‑1 (MQ=255)         cTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCCCCGTTGGCGCCAGGCAGGgt                                                                                                                                     >  2:264923/1‑150 (MQ=255)         cTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGgt                                                                                                                                     >  2:807345/1‑150 (MQ=255)               cAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTtcat                                                                                                                               <  1:877659/150‑1 (MQ=255)                    gTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAAAAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTc                                                                                                                          <  2:404173/150‑1 (MQ=255)                        tACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTcgcgc                                                                                                                      >  2:1385528/1‑150 (MQ=255)                                   cACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCgg                                                                                                           <  2:857576/150‑1 (MQ=255)                                            aaGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCAc                                                                                                  <  1:334940/150‑1 (MQ=255)                                                aGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTcc                                                                                              >  1:829343/1‑150 (MQ=255)                                                aGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTcc                                                                                              >  2:1337432/1‑150 (MQ=255)                                                                     ggCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATc                                                                         <  2:1419595/150‑1 (MQ=255)                                                                        aGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCggg                                                                      <  2:559504/150‑1 (MQ=255)                                                                          aaaCCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGt                                                                    <  1:264923/150‑1 (MQ=255)                                                                           aaCCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTg                                                                   >  2:1307029/1‑150 (MQ=255)                                                                                    cATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCg                                                          <  2:829343/150‑1 (MQ=255)                                                                                     aTGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAg                                                                                        <  2:1008415/119‑1 (MQ=255)                                                                                     aTGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAg                                                                                        >  1:1008415/1‑119 (MQ=255)                                                                                                  gcATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGa                                            >  2:1031393/1‑150 (MQ=255)                                                                                                    aTGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACt                                          >  1:936906/1‑150 (MQ=255)                                                                                                        gAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTGCGAGGTGGTGACAACGCCGGCCATCTCCATCGACTCGAt                                      >  1:74146/1‑150 (MQ=255)                                                                                                         aaTCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGTTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATc                                              <  2:975056/141‑1 (MQ=255)                                                                                                         aaTCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGTTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATc                                              >  1:975056/1‑141 (MQ=255)                                                                                                            ccTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATg                                  >  1:1098989/1‑150 (MQ=255)                                                                                                             cTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGTGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATgc                                 <  2:319535/150‑1 (MQ=255)                                                                                                             cTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGGGACAACGCCGGCCATCTCCATCGACTCGATCATgc                                 >  1:1148843/1‑150 (MQ=255)                                                                                                               cATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATgcgc                               <  1:1337432/150‑1 (MQ=255)                                                                                                                aTTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATgcgcg                              >  2:640192/1‑150 (MQ=255)                                                                                                                 ttGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATgcgcgc                             <  1:1307029/150‑1 (MQ=255)                                                                                                                          gAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGATGAATGCCCGCATGTGCCGCCCGGGGCTATCAATTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGtt                    <  1:1385528/150‑1 (MQ=255)                                                                                                                           aaaaGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTg                   >  1:1292173/1‑150 (MQ=255)                                                                                                                             cctgCCTGGCGCCAACGGTGGCGCCAGGCCGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTa                 >  1:549710/4‑150 (MQ=255)                                                                                                                              ctgCCTGGCGCCAACGGTGGCGCCAGGCCGGGTGTTCATCAGTCGCGAGGTCCGTCCGGGGGTATCACTTCCAGCGAGCCGTTGCTGATAATCGCGTGGAGAGCACCGGCCATCTCCATCGACTCGATCTTGCGCGCGGCACGGTTGttg                >  2:691665/3‑148 (MQ=255)                                                                                                                                  cTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGa            >  2:1269460/1‑150 (MQ=255)                                                                                                                                   tGGCGCCAACGGTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGAt           >  1:887750/1‑150 (MQ=255)                                                                                                                                    ggCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGATc          <  1:807345/150‑1 (MQ=255)                                                                                                                                       gcCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGATCTTc       <  2:282463/150‑1 (MQ=255)                                                                                                                                       gcCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGATCTTc       >  1:1043348/1‑150 (MQ=255)                                                                                                                                        ccACAGTTGGCGCCCGGAAGGGTGTTCATGCGTAGCTAGAGCAGCCTGAAGCTATCACTACCGGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGATCTTCa      <  2:74146/150‑1 (MQ=255)                                                                                                                                         cACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGATCTTCAg     >  1:71383/1‑150 (MQ=255)                                                                                                                                         cACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGAGCTTCAg     >  1:524174/1‑150 (MQ=255)                                                                                                                                         cACCGTTGGCGCCAGACAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGATCTTCAg     >  1:926813/1‑150 (MQ=255)                                                                                                                                            cGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGATCTTCAGCtt  <  2:1098989/150‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                    TCCAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGATCTTCAGCTT  >  NC_002947/4512368‑4512656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑