| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,545,358 | C→A | 8.5% | K164N (AAG→AAT) | hyuC ← | bifunctional N‑carbamoyl‑beta‑alanine amidohydrolase/allantoine amidohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,545,358 | 0 | C | A | 8.5% | 187.1 / 5.7 | 59 | K164N (AAG→AAT) | hyuC | bifunctional N‑carbamoyl‑beta‑alanine amidohydrolase/allantoine amidohydrolase |
| Reads supporting (aligned to +/- strand): ref base C (27/27); new base A (2/3); total (29/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01 | |||||||||||
TTGGCCTGGTCCTCAAGGATCGGCCCCTGCTCGATATGCGCTTCGAAATACGCCCCCACCGGGTGCCCCGACACAGCACGCGGACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCAGACCCCATCATGCATGGCGCAAAACGCGAACCTTCTTCGTTGGTCCACACCACCACTTCGAGCGACGCTTCGGTTTCCACGCCCAGGTCGTTGAGGGTGCGG > NC_002947/4545218‑4545505 | tgggCCTGATCCTCAAGGATCGGCCCCTGCTCGATATGCGCTTCGAAATACGCCCCCACCGGGTGCCCCGACACAGCACGCGGACCGGCGGAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGAAT‑CACGCTTGGCCAgg > 2:161543/3‑150 (MQ=255) tGGCCTGGTCCTCAAGGATCGGCCCCTGCTCGATATGCGCTTCGAAATACGCCCCCACCGGGTGCCCCGACACAGCACGCGGACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAggg < 2:1367579/150‑1 (MQ=255) ggTCCTCAAGGATCGGCCCCTGCTCGATATGCGCTTCGAAATACGCCCCCACCGGGTGCCCCGACACAGCACGCGGACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCtt > 1:722121/1‑150 (MQ=255) gTCCTCAAGGATCGGCCCCTGCTCGATATGCGCTTCGAAATACGCCCCCACCGGGTGCCCCGACACAGCACGCGGACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTc > 2:1003347/1‑150 (MQ=255) ccTCAAGGATCGGCCCCTGCTCGATATGCGCTTCGAAATACGCCCCCACCGGGTGCCCCGACACAGCACGCGGACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCa < 1:1003347/150‑1 (MQ=255) tCAAGGATCGGCCCCTGCTCGATATGCGCTTCGAAATACGCCCCCACCGGGTGCCCCGACACAGCACGCGTACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAgg > 2:525526/1‑150 (MQ=255) tCAAGGATCGGCCCCTGCTCGATATGCGCTTCGAAATACGCCCCCACCGGGTGCCCCGACACAGCACGCGGACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAgg < 1:820601/150‑1 (MQ=255) tCAAGGATCGGCCCCTGCTCGATATGCGCTTCGAAATACGCCCCCACCGGGTGCCCCGACACAGCACGCGGACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAgg < 1:601078/150‑1 (MQ=255) cAAGGATCGGCCCCTGCTCGATATGCGCTTCGAAATACGCCCCCACCGGGTGCCCCGACACAGCACGCGGACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAggg > 1:985510/1‑150 (MQ=255) ggATCGGCCCCTGCTCGATATGCGCTTCGAAATACGCCCCCACCGGGTGCCCCGACACAGCACGCGGACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGa > 1:480782/1‑150 (MQ=255) gCCCCTGCTCGATATGCGCTTCGAAATACGCCCCCACCGGGTGCCCCGACACAGCACGCGGACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGc > 1:51099/1‑150 (MQ=255) gCCCCTGCTCGATATGCGCTTCGAAATACGCCCCCACCGGGTGCCCCGACACAGCACGCGGACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGc > 1:498341/1‑150 (MQ=255) tGCTCGATATGCGCTTCGAAATACGCCCCCACCGGGTGCCCCGACACAGCACGCGGACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCgg < 2:1264950/148‑1 (MQ=255) tGCTCGATATGCGCTTCGAAATACGCCCCCACCGGGTGCCCCGACACAGCACGCGGACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCgg > 1:1264950/1‑148 (MQ=255) tcgaagtggtggtgtggaccaacgaagaaggttcgcgttttgcgccatgcatgatggggtctggggtattcgccggcaagttcaccctggaaGACACCCTGGGCAT‑CGCGCTTGGCCAGGgt > 2:69006‑M1/93‑122 (MQ=255) tcgaagtggtggtgtggaccaacgaagaaggttcgcgttttgcgccatgcatgatggggtctggggtattcgccggcaagttcaccctggaaGACACCCTGGGCAT‑CGCGCTTGGCCAGGgt < 1:69006‑M1/30‑1 (MQ=255) gATATGCGCTTCGAAATACGCCCCCACCGGGTGCCCCGACACAGCACGCGGACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAgg < 1:317159/118‑1 (MQ=255) gATATGCGCTTCGAAATACGCCCCCACCGGGTGCCCCGACACAGCACGCGGACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAgg > 2:317159/1‑118 (MQ=255) tatGCGCTTCGAAATACGCCCCCACCGGGTGCCCCGACACAGCACGCGGACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATAccc < 2:51099/150‑1 (MQ=255) tGCGCTTCGAAATACGCCCCCACCGGGTGCCCCGACACAGCACGCGGACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCa < 1:406405/150‑1 (MQ=255) tGCGCTTCGAAATACGCCCCCACCGGGTGCCCCGACACAGCACGCGGACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCa < 2:722121/150‑1 (MQ=255) ttCGAAATACGCCCCCACCGGGTGCCCCGACACAGCACGCGGACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATAccc > 2:1090749/1‑143 (MQ=255) ttCGAAATACGCCCCCACCGGGTGCCCCGACACAGCACGCGGACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATAccc < 1:1090749/143‑1 (MQ=255) ttCGAAATACGCCCCCACCGGGTGCCCCGACACAGCACGCGGACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCAGAccc > 2:734286/1‑150 (MQ=255) tACGCCCCCACCGGGTGCCCCGACACAGCACGCGGACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCAGACCCCATcatg > 1:1248640/1‑150 (MQ=255) gCCCCCACCGGGTGCCCCGACACAGCACGCGGACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCAGACCCCATcatgcat > 1:1370800/1‑150 (MQ=255) ccccACCGGGTGCCCCGACACAGCACGCGGACCGGCGTAGCCAATGGAGTTCAACGCTTCTCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCAGACCCCATcatgcatg > 1:754694/1‑149 (MQ=255) ccccACCGGGTGCCCCGACACAGCACGCGGACCGGCGTAGCCAATGGAGTTCAACGCTTCTCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCAGACCCCATcatgcatg < 2:754694/149‑1 (MQ=255) ccccACCGGGTGCCCCGACACAGCACGCGGACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCAGACCCCATCATGCATgg > 1:129580/1‑150 (MQ=255) ccGACACAGCACGCGGACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAgg < 1:231306/95‑1 (MQ=255) ccGACACAGCACGCGGACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAgg > 2:231306/1‑95 (MQ=255) cGACACAGCACGCGGACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCa > 2:451866/1‑92 (MQ=255) cGACACAGCACGCGGACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCa < 1:451866/92‑1 (MQ=255) aCGAGGACCGGCGTAGCCAATGGAGTGCAACGCTTCGCCAACGCTGACACCCTGAGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCAGCCCCCATCATGCATGGCGCAAAACGCGAACCTTCTTCGtt < 1:655116/150‑1 (MQ=255) cgcgGACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCAGACCCCATCATGCATGGCGCAAAACGCGAACCTTCTTCGTTg < 2:480782/150‑1 (MQ=255) gcgGACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAggg < 1:1147700/72‑1 (MQ=255) gcgGACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAggg > 2:1147700/1‑72 (MQ=255) cGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCAGACCCCATCATGCATGGCGCAAAACGCGAACCTTCTTCGTTGGTc < 1:1032423/146‑1 (MQ=255) cGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCAGACCCCATCATGCATGGCGCAAAACGCGAACCTTCTTCGTTGGTc > 2:1032423/1‑146 (MQ=255) gCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCAGACCCCATCATGCATGGCGCAAAACGCGAACCTTCTTCGTTGGTCCAcacc > 2:1264651/1‑150 (MQ=255) cGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCAGACCCCATCATGCATGGCGCAAAACGCGAACCTTCTTCGTTGGTCCAcacca < 2:129580/150‑1 (MQ=255) gTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCAGACCCCATCATGCATGTCGCAAAACGCGAACCTTCTTCGTTGGTCCAcaccac < 2:985510/150‑1 (MQ=255) gCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGaa > 2:1195735/1‑87 (MQ=255) gCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGaa < 1:1195735/87‑1 (MQ=255) atgtggTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCAGACCCCGTCATGCATGGCGCAAAACGCGAACCTTCTTCGTTGGTCCACACCACCACTTCg > 2:1066355/6‑150 (MQ=255) tGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCAGACCCCATCATGCATGGCGCAAAACGCGAACCTTCTTCGTTGGTCCACACCACCACTTCGa < 1:91336/150‑1 (MQ=255) tGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCAGACCCCATCATGCATGGCGCAAAACGCGAACCTTCTTCGTTGGTCCACACCACCACTTCGa < 1:620236/150‑1 (MQ=255) gttcaccctggaaGACACCCTGGCCAAGCGCGAT‑GCCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCAGACCCCATcatgcat < 2:535795‑M1/79‑1 (MQ=255) gttcaccctggaaGACACCCTGGCCAAGCGCGAT‑GCCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCAGACCCCATcatgcat > 1:535795‑M1/14‑92 (MQ=255) ttCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCAGACCCCATCATGCATGGCGCAAAACGCGAACCTTCTTCGTTGGTCCacac < 2:1032840/126‑1 (MQ=255) ttCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCAGACCCCATCATGCATGGCGCAAAACGCGAACCTTCTTCGTTGGTCCacac > 1:1032840/1‑126 (MQ=255) tggaaGACACCCTGGCCAAGCGCGAT‑GCCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCAGACCCCATCATGCATGGCGCAAAACGCGAACCTTCTTCGTTg > 2:476964‑M1/6‑111 (MQ=255) tggaaGACACCCTGGCCAAGCGCGAT‑GCCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCAGACCCCATCATGCATGGCGCAAAACGCGAACCTTCTTCGTTg < 1:476964‑M1/106‑1 (MQ=255) tggaaGACACCCTGGCCAAGCGCGAT‑GCCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCAGACCCCATCATGCATGGCGCAAAACGCGAACCTTCTTCGTTGGTCCACACCACCACTTCGAGCGACGCTTCGGTTTCCACg < 2:182926‑M1/145‑1 (MQ=255) cGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCAGACCCCATCATGCATGGCGCAAAACGCGAACCTTCTTCGTTGGTCCACACCACCACTTCGAGCGACGCTTCGGTTTCCACGc > 1:1103206/1‑150 (MQ=255) ACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCAGACCCCATCATGCATGGCGCAAAACGCGAACCTTCTTCGTTGGTCCACACCACCACTTCGCCCGACGCTTCGGTTTCTACCCCCAGGTc < 2:139348‑M1/150‑1 (MQ=255) CCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCAGACCCCATCATGCATGGCGCAAAACGCGAACCTTCTTCGTTGGTCCACACCACCACTTCGAGCTACGCTTCGGTTTCCACGCCCAGGTCg < 2:1008308‑M1/150‑1 (MQ=255) GGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCAGACCCCATCATGCATGGCGCAAAACGCGAACCTTCTTCGTTGGTCCACACCACCACTTCGAGCGACGCTTCGGTTTCCACGCCCAGGTCGTTGAg > 1:734286‑M1/1‑150 (MQ=255) cgcgCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCAGACCCCATCATGCATGGCGCAAAACGCGAACCTTCTTCGTTGGTCCACACCACCACTTCGAGCGACGCTTCGGTTTCCACGCCCAGGTCGTTGAGGGTGc < 2:498341/150‑1 (MQ=255) gcgcTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCAGACCCCATCATGCATGGCGCAAAACGCGAACCTTCTTCGTTGGTc > 1:1358330/1‑94 (MQ=255) gcgcTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCAGACCCCATCATGCATGGCGCAAAACGCGAACCTTCTTCGTTGGTc < 2:1358330/94‑1 (MQ=255) cgcTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGGATACCGCAGACCCCATCATGCATGGCGCAAAACGCGAACCTTCTTCGTTGGTCCACACCACCACTTCGAGCGACGCTTCGGTTTCCACGCCCAGGTCGTTGAGGGTGCgg > 2:984743/1‑150 (MQ=255) | TTGGCCTGGTCCTCAAGGATCGGCCCCTGCTCGATATGCGCTTCGAAATACGCCCCCACCGGGTGCCCCGACACAGCACGCGGACCGGCGTAGCCAATGGAGTTCAACGCTTCGCCAACGCTGACACCCTGGGCAT‑CGCGCTTGGCCAGGGTGTCTTCCAGGGTGAACTTGCCGGCGAATACCCCAGACCCCATCATGCATGGCGCAAAACGCGAACCTTCTTCGTTGGTCCACACCACCACTTCGAGCGACGCTTCGGTTTCCACGCCCAGGTCGTTGAGGGTGCGG > NC_002947/4545218‑4545505 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |