Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	390,999	T→A	9.0%	Y424N (TAC→AAC)	soxA →	sarcosine oxidase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	390,999	0	T	A	9.0%	199.8 / 8.9	67	Y424N (TAC→AAC)	soxA	sarcosine oxidase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (33/28);  new base A (3/3);  total (36/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

GCATACTCACCTCCAGCGCCGTGATCGAGGCCAAAGGCAGCAAGCACGTCACCGGCGCCCGTGTGGC‑GGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGGTATCAACGGCGTGTATGCCTTGGGCGATGTCATTGC  >  NC_002947/390853‑391147                                                                                                                                                    |                                                                                                                                                     gCATACTCACCTCCAGCGCCGTGATCGAGGCCAAAGGCAGCAAGCACGTCACCGGCGCCCGTGTGGC‑GGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACa                                                                                                                                                   <  2:1369238/150‑1 (MQ=255)  cATACTCACCTCCAGCGCCGTGATCGAGGCCAAAGGCAGCAAGCACGTCACCGGCGCCCGTGTGGC‑GGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAg                                                                                                                                                  >  1:489554/1‑150 (MQ=255)       tCACCTCCAGCGCCGTGATCGAGGCCAAAGGCAGCAAGCACGTCACCGGCGCCCGTGTGGC‑GGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGa                                                                                                                                             >  2:735214/1‑150 (MQ=255)         tcttCCAGCGCCGTGCTCGAGGCCAAAGGCAGCAAGCACGTCACCGGCGCCCGTGTGGC‑GGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATc                                                                                                                                           <  2:684196/147‑1 (MQ=255)         aCCTCCAGCGCCGTGATCGAGGCCAAAGGCAGCAAGCACGTCACCGGCGCCCGTGTGGC‑GGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGcccatga                                                                                                                                           >  2:1218065/1‑146 (MQ=255)          ccTCCAGCGCCGTGATCGAGGCCAAAGGCAGCAAGCACGTCACCGGCGCCCGTGTGGC‑GGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCTGTAACCGCCCGagga                                                                                                                                          <  2:796973/150‑4 (MQ=255)             ccAGCGCCGTGATCGAGGCCAAAGGCAGCAAGCACGTCACCGGGGGCTGTGTGGC‑GGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCgaga                                                                                                                                       >  1:684196/1‑147 (MQ=255)             ccAGCGCCGTGATCGAGGCCAAAGGCAGCAAGCACGTCACCGGC‑CACTTATCGCTGGCGTGCGATGTGCAGGCGACCAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGc                                                                                                                                       <  1:1028903/150‑1 (MQ=255)                gcgcCGTGATCGAGGCCAAAGGCAGCAAGCACGTCACCGGCGCCCGTGTGGC‑GGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCAcc                                                                                                                                    >  1:702448/1‑150 (MQ=255)                      tGATCGAGGCCAAAGGCAGCAAGCACGTCACAGGCGCCCGTGTGGC‑GGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCtt                                                                                                                              >  1:1177059/1‑150 (MQ=255)                       gATCGAGGCCAAAGGCAGCAAGCACGTCACCGGCGCCCGTGTGGC‑GGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTc                                                                                                                             >  2:918722/1‑150 (MQ=255)                         tCGAGGCCAAAGGCAGCAAGCACGTCACCGGCGCCCGTGTGGC‑GGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGc                                                                                                                           <  1:262187/150‑1 (MQ=255)                          cGAGGCCAAAGGCAGCAAGCACGTCACCGGCGCCCGTGTGGC‑GGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCa                                                                                                                          >  1:635185/1‑150 (MQ=255)                           gAGGCCAAAGGCAGCAAGCACGTCACCGGCGCCCGTGTGGC‑GGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTg                                                                                                                                        >  2:508445/1‑135 (MQ=255)                           gAGGCCAAAGGCAGCAAGCACGTCACCGGCGCCCGTGTGGC‑GGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTg                                                                                                                                        <  1:508445/135‑1 (MQ=255)                                 aaaGGCAGCAAGCACGTCACCGGCGCCCGTGTGGC‑GGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGc                                                                                                                   <  1:1254890/150‑1 (MQ=255)                                  aaGGCAGCAAGCACGTCACCGGCGCCCGTGTGGC‑GGCGCTGGATGGCCATGCGCACAAAGTCACAAGCCAAGGCGAAGTCATTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTAAAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCg                                                                                                                  >  2:984823/1‑150 (MQ=255)                                         caagcaCGTCACCGGCGCCCGTGTGGC‑GGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGccc                                                                                                           <  2:117826/150‑1 (MQ=255)                                            gcaCGTCACCGGCGCCCGTGTGGC‑GGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGt                                                                                                        <  2:489554/150‑1 (MQ=255)                                                 tCACCGGCGCCCGTGTGGC‑GGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGACCGcccaggt                                                                                                       >  2:1091645/1‑142 (MQ=255)                                                 ccACCGGCGCCCGTGTGGC‑GGCGACCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGACCGcccaggt                                                                                                       <  1:1091645/145‑5 (MQ=255)                                                   aCCGGCGCCCGTGTGGC‑GGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGt                                                                                                 <  1:1323408/150‑1 (MQ=255)                                                                      gcgATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTc                                                                               >  1:496599/1‑150 (MQ=255)                                                                            gatGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGc                                                                                                   >  2:624247/1‑124 (MQ=255)                                                                            gatGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGc                                                                                                   <  1:624247/124‑1 (MQ=255)                                                                            gatGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCg                                                                         <  2:1177059/150‑1 (MQ=255)                                                                             atGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCgg                                                                        >  2:1036360/1‑150 (MQ=255)                                                                                   aGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCTGTAACCGCCCGag                                                                                                                                            >  1:1371800/1‑75 (MQ=255)                                                                                   aGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCTGTAACCGCCCGag                                                                                                                                            <  2:1371800/76‑2 (MQ=255)                                                                                    ggCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATgc                                                                 <  1:735214/150‑1 (MQ=255)                                                                                      cgcACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCGCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATgcgc                                                               <  2:756054/150‑1 (MQ=255)                                                                                       gcACAAAGTCACCAGCCCAGGCGAAATCCTTGAGTGCGACCTGATCGACTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGCTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGACGGGCGATGcacc                                                              >  2:190862/1‑150 (MQ=255)                                                                                           aaaGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAg                                                          >  2:358625/1‑150 (MQ=255)                                                                                                 aCCAGCCCAGGCGAAGTCCTTGAGTGTGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGt                                                    >  1:226453/1‑150 (MQ=255)                                                                                                 aCCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTgacga                                                                                            >  2:99135/1‑110 (MQ=255)                                                                                                 aCCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTgacga                                                                                            <  1:99135/110‑1 (MQ=255)                                                                                                   cAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGa                                                  <  2:88670/150‑1 (MQ=255)                                                                                                     gCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCTGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGt                                                <  1:1159097/150‑1 (MQ=255)                                                                                                           gCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGa                                                  <  1:471402/142‑1 (MQ=255)                                                                                                           gCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGa                                                  >  2:471402/1‑142 (MQ=255)                                                                                                           gCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCg                                          >  2:327717/1‑150 (MQ=255)                                                                                                             gAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTGCAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCggt                                        >  1:1358991/1‑150 (MQ=255)                                                                                                              ccgatcTTGAGTGCGACATGATCGACACCATCGACGGTTACATCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCggtg                                       <  2:866736/145‑1 (MQ=255)                                                                                                                   cTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACAt                                                                                         >  2:20080/1‑95 (MQ=255)                                                                                                                   cTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACAt                                                                                         <  1:20080/95‑1 (MQ=255)                                                                                                                   cTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGGTATc                                  >  2:1343598/1‑150 (MQ=255)                                                                                                                    ttGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCggtgg                                      >  2:1399609/1‑145 (MQ=255)                                                                                                                    ttGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCggtgg                                      <  1:1399609/145‑1 (MQ=255)                                                                                                                    ttGAGTGCGACCTGATCGCCTCCTAGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGt                                                    <  2:930503/131‑1 (MQ=255)                                                                                                                    ttGAGTGCGACCTGATCGCCTCCTAGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGt                                                    >  1:930503/1‑131 (MQ=255)                                                                                                                      gAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCggtggagatcgg                               >  1:866736/1‑143 (MQ=255)                                                                                                                      gAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGGTATCAAc                               <  1:354637/150‑1 (MQ=255)                                                                                                                         tGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGGTATCAACGGc                            >  1:487130/1‑150 (MQ=255)                                                                                                                         tGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGGTATCAACGGc                            <  1:984823/150‑1 (MQ=255)                                                                                                                            gACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGGTATCAACGGCgtg                         >  2:1233111/1‑150 (MQ=255)                                                                                                                               cTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCggt                                        <  2:833366/132‑1 (MQ=255)                                                                                                                               cTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCggt                                        >  1:833366/1‑132 (MQ=255)                                                                                                                                    tgcacgatcGGGCTGTAACCGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGGTATCAACGGCGTGTATGCCtt                 >  1:1229524/8‑150 (MQ=255)                                                                                                                                       ctcctcGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGGTATCAACGGCGTGTATGCCTTggg              >  2:286094/1‑150 (MQ=255)                                                                                                                                         gatcGGGCTGTAACCGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCggtg                                       >  2:630357/3‑123 (MQ=255)                                                                                                                                         gatcGGGCTGTAACCGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCggtg                                       <  1:630357/121‑1 (MQ=255)                                                                                                                                           tcGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCggtg                                       >  2:359668/1‑121 (MQ=255)                                                                                                                                           tcGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCggtg                                       <  1:359668/121‑1 (MQ=255)                                                                                                                                             gggCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGc                                              <  2:658676/112‑1 (MQ=255)                                                                                                                                             gggCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGc                                              >  1:658676/1‑112 (MQ=255)                                                                                                                                                  gTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCg                                                                              <  1:985326/75‑1 (MQ=255)                                                                                                                                                  gTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCg                                                                              >  2:985326/1‑75 (MQ=255)                                                                                                                                                   ttACAGCCCGATCGTGGTCCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGGTATCAACGGCGTGTATGCCTTGGGCGATGTCATTGc  <  2:516398/150‑1 (MQ=255)                                                                                                                                                   ttACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGGTATCAACGGCGTGTATGCCTTGGGCGATGTCATTGc  <  1:1395609/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GCATACTCACCTCCAGCGCCGTGATCGAGGCCAAAGGCAGCAAGCACGTCACCGGCGCCCGTGTGGC‑GGCGATCGATGTGCAGGCGCACAAAGTCACCAGCCCAGGCGAAGTCCTTGAGTGCGACCTGATCGCCTCCTCGGGCGGTTACAGCCCGATCGTGCACCTGGCTTCGCACCTGGGCGGTCGCCCGGTATGGCGTGACGACATCCTTGGCTTCGTGCCGGGCGATGCGCCGCAGAAGCGTGAGTGCGTCGGTGGTATCAACGGCGTGTATGCCTTGGGCGATGTCATTGC  >  NC_002947/390853‑391147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑