| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,564,769 | A→G | 9.9% | intergenic (‑865/‑33) | vgrG‑III ← / → glgA | type VI secretion system protein/glycogen synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,564,769 | 0 | A | G | 9.9% | 164.9 / 7.7 | 61 | intergenic (‑865/‑33) | vgrG‑III/glgA | type VI secretion system protein/glycogen synthase |
| Reads supporting (aligned to +/- strand): ref base A (28/26); new base G (3/3); total (32/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-01 | |||||||||||
CAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAGCCGCTCACCCCGGATTTCGCCACGACAGGCAAGGCACCCGGCGCCCAGCGCCAGCACAA > NC_002947/4564621‑4564917 | cAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAg < 1:1330846/150‑1 (MQ=255) accaACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCTGGCACCCgtggc < 2:1230697/149‑5 (MQ=255) accaACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCTGGCACCCgtggc > 1:1230697/1‑145 (MQ=255) accaACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCaa > 2:1220607/1‑150 (MQ=255) aacAGCCCAATCGCTTTCGGCCGGAACGCCGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAggg > 2:821555/1‑150 (MQ=255) tCGCTTTCGGCCGGAACGCCGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGaa < 1:1158768/150‑1 (MQ=255) tCGCTTTCGGCCGGAACGCCGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGaa < 1:463213/150‑1 (MQ=255) tCGCTTTCGGCCGGAACGCCGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGaa < 1:1220607/150‑1 (MQ=255) gCTTTCGGCCGGAACGCCGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGt < 1:821555/150‑1 (MQ=255) cGGAACGCCGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGAACCCGACCAAGCATAGGGACGGAGAGAAGTATGATAAGt > 1:75687/1‑150 (MQ=255) aaCGCCGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGcc < 1:710498/150‑1 (MQ=255) gCCGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATc > 1:1047017/1‑141 (MQ=255) gCCGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATc < 2:1047017/141‑1 (MQ=255) cGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGt < 2:1217495/150‑1 (MQ=255) cGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGt > 1:1189181/1‑150 (MQ=255) cGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGt < 1:927518/150‑1 (MQ=255) cGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGt < 1:40887/150‑1 (MQ=255) gcgcCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGc < 2:1189181/150‑1 (MQ=255) tcgtgcgcgCACGCACGGCACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACGTCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGc < 1:25561/146‑1 (MQ=255) tcgtgcgcgCACGCACGGCACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACGTCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGc > 2:25561/2‑147 (MQ=255) ccGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTc < 1:5632/150‑1 (MQ=255) gcgcgcACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGAc < 2:714553/150‑1 (MQ=255) gcACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCCGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATc > 1:1381028/1‑119 (MQ=255) gcACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATc < 2:1381028/119‑1 (MQ=255) aCGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCa > 1:327360/1‑79 (MQ=255) aCGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCa < 2:327360/79‑1 (MQ=255) acaGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAg < 1:1328333/92‑1 (MQ=255) acaGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAg > 2:1328333/1‑92 (MQ=255) acaGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAAcc < 2:240974/150‑1 (MQ=255) aTGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCttt < 1:1173236/136‑1 (MQ=255) aTGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCttt > 2:1173236/1‑136 (MQ=255) ggAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACcgc < 2:62447/150‑1 (MQ=255) aggCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAAGAGCGCCTCGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAg > 2:764506/3‑150 (MQ=255) cACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACcg < 1:1251892/143‑1 (MQ=255) cACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACcg > 2:1251892/1‑143 (MQ=255) cACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACAAAGCAAAGGGACGGGGAGAAGTATGATCAGGACGGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAGCCg > 2:691854/1‑150 (MQ=255) aTTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCg < 1:240930/124‑1 (MQ=255) aTTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCg > 2:240930/1‑124 (MQ=255) ttCAGCCAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGACCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAGCCGCTCACCCCGGAt > 2:1384244/1‑150 (MQ=255) aagcaGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAGCCGCTCACCCCGGATTTCGcc > 1:1088588/1‑150 (MQ=255) gcaGAGAGAACGAGCACGAAACATCGCCAAGGGTGCCAGCACCCGGCCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGACTCACGTAGACGCTTTCAAAGCGGATAACTGCGAGCCGCTCACCCCGGGTTTCGCCAc > 2:202207/1‑150 (MQ=255) agagaACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAGCCGCTCACCCCGGATTTCGCCACGACa > 1:113201/1‑150 (MQ=255) agagaACCAGCACGAAACATCGCCACGGGGGCCAGAACCGGAGGATGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCAGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAGCCGCTCACCCCGGATTTCGCCACGACa > 2:275766/1‑150 (MQ=255) ccAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCaaa > 1:691769/1‑102 (MQ=255) ccAGCACGAAACATCGCCAAGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCaaa < 2:691769/102‑1 (MQ=255) cAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGt > 1:191558/1‑68 (MQ=255) cAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGt < 2:191558/68‑1 (MQ=255) aGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAGc < 1:265783/116‑1 (MQ=255) aGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAGc > 2:265783/1‑116 (MQ=255) cACGAAACATCGCCACGGGTGCCAGCACCCGACCaa < 2:1369874/36‑1 (MQ=255) cACGAAACATCGCCACGGGTGCCAGCACCCGACCaa > 1:1369874/1‑36 (MQ=255) cGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAGCCGCTCACCCCGGATTTCGc > 1:623386/1‑131 (MQ=255) cGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAGCCGCTCACCCCGGATTTCGc < 2:623386/131‑1 (MQ=255) gAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAGCCGCTCACCCCGGATTTCGCCACGACAGGCAAGGCACCCg > 1:51808/1‑150 (MQ=255) gAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAGCCGCTCACCCCGGATTTCGCCACGACAGGCAAGGCACCCg > 1:306093/1‑150 (MQ=255) aaaCATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAGCCGCTCACCCCGGATTTCGCCACGACAGGCAAGGCACCCgg > 2:508647/1‑150 (MQ=255) ccACGGGTGCCAGCACCCGACCAAGCATAGGGACGGTGTGACGAAACTTGCGTTAGCCAGGCGATCCTCACTGATACGCTTTCAAACCGGATAACCGCGAGCCGCTCACCCCGGATTTCGCCACGACAGGCAAGGCACCCGGCGCCCAgc > 1:739121/1‑150 (MQ=255) cACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAGCCGCTCACCCCGGATTTCGCCACGACAGGCAAGGCACCCGGCGCCCAgcg > 2:617140/1‑150 (MQ=255) tcGGGTGCTGGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGa < 1:1018340/88‑1 (MQ=255) tcGGGTGCTGGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGa > 2:1018340/2‑89 (MQ=255) tcGGGTGCTGGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAGCCGCTCACCCCg < 2:1269886/110‑1 (MQ=255) tcGGGTGCTGGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAGCCGCTCACCCCg > 1:1269886/2‑111 (MQ=255) cAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGGAGACGCTTTCAAACCGGATAACCGCGAGCCGCTCACCCCGGATTTCGCCACGACAGGCAAGGCACCCGGCGCCCAGCGCCAGCACaa > 1:116956/1‑150 (MQ=255) | CAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAGCCGCTCACCCCGGATTTCGCCACGACAGGCAAGGCACCCGGCGCCCAGCGCCAGCACAA > NC_002947/4564621‑4564917 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |