Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,589,941 G→A 8.9% A193T (GCG→ACG)  mccB → methylcrotonyl‑CoA carboxylase biotin‑containing subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,589,9410GA 8.9% 125.3 / 3.3 45A193T (GCG→ACG) mccBmethylcrotonyl‑CoA carboxylase biotin‑containing subunit beta
Reads supporting (aligned to +/- strand):  ref base G (22/19);  new base A (2/2);  total (24/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

GCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTGCGCCAGCAGGCGACCATCTTCCTCGCCGGCCCGCCGCTGGTGAAAGCCGCCACCGGCGAAGTGGTGAGCGCCGAAGACCTCGGCGGCGCCGATGTGCATTGC  >  NC_002947/4589801‑4590081
                                                                                                                                            |                                                                                                                                            
gcggcgCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGc                                                                                                                                     >  1:945847/1‑150 (MQ=255)
     gcCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGcc                                                                                                                                >  2:603592/1‑150 (MQ=255)
       cAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGCCGGCACGTACGCGCCgcca                                                                                                                                <  1:1280505/148‑4 (MQ=255)
       cAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGCCGGCACGTACGCGCCgcca                                                                                                                                >  2:1280505/1‑145 (MQ=255)
        aaCTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATg                                                                                                                             >  2:352888/1‑150 (MQ=255)
          cTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGc                                                                                                                           <  2:767543/150‑1 (MQ=255)
             gCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCcga                                                                                                                        >  2:634681/1‑150 (MQ=255)
                ccGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCcgacga                                                                                                                     >  1:1118677/1‑150 (MQ=255)
                ccGCCAGGACGAAGTGTTCCCCGACCGCGAGCATCTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCcgacga                                                                                                                     >  1:658467/1‑150 (MQ=255)
                            aGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGt                                                                                                         >  1:1419205/1‑150 (MQ=255)
                                  ccccGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTGCGCca                                                                                                   >  2:993388/1‑150 (MQ=255)
                                        ccGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTGCGCCAGCAGGc                                                                                             <  2:1248394/150‑1 (MQ=255)
                                            gAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCGTGGTGCGCcagcag                                                                                               <  2:381754/144‑1 (MQ=255)
                                            gAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCGTGGTGCGCcagcag                                                                                               >  1:381754/1‑144 (MQ=255)
                                             aGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTGCGCCAGCAGGCGACCa                                                                                        <  2:35096/150‑1 (MQ=255)
                                                  ttCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCGTGGTGCGCCAGCAGGCGACCATCTTc                                                                                   <  2:484377/150‑1 (MQ=255)
                                                           aTTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTGCGCCAGCAGGCGACCATc                                                                                      <  2:949502/138‑1 (MQ=255)
                                                           aTTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTGCGCCAGCAGGCGACCATc                                                                                      >  1:949502/1‑138 (MQ=255)
                                                            ttttCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTGCGCCAGCAGGCGACCATCTTCCTCGCCGGcc                                                                         >  1:111820/1‑150 (MQ=255)
                                                                cttcAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTGCGCCAGCAGGCGACCATCTTCCTCGCCGGCccgcc                                                                     >  1:46921/1‑150 (MQ=255)
                                                                 ttcAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTGCGCCAGCAGGCGACCATCTTCCTCGCCGGCccgccg                                                                    >  2:794948/1‑150 (MQ=255)
                                                                    aaCCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTGCGCCAGCAGGCGACCATCTTCCTCGCCGGCCCGCCGCTg                                                                 <  2:945847/150‑1 (MQ=255)
                                                                     aCCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCACGTACGCGCCgcca                                                                                                                                <  1:362911/86‑4 (MQ=255)
                                                                     aCCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCACGTACGCGCCgcca                                                                                                                                >  2:362911/1‑83 (MQ=255)
                                                                      ccAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTGCGCCAGCAGGCGACCATCTTCCTCGCCGGCCCGCCGCTGGt                                                               >  2:644377/1‑150 (MQ=255)
                                                                        aGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGCCACGCACAGCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTGCGCCAGCAGGCGACCATCTTCCTCGCCGGCCCGCCGCTGGTGa                                                             >  2:1058866/1‑150 (MQ=255)
                                                                          gCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTGCGCCAGCAGGCGACCATCTTCCTCGCCGGCCCGCCGCTGGTGaaa                                                           <  2:111820/150‑1 (MQ=255)
                                                                          gCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTGCGCCAGCAGGCGACCATCTTCCTCGCCGGCCCGCCGCTGGTGaaa                                                           <  1:644377/150‑1 (MQ=255)
                                                                          gCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTGCGCCAGCAGGCGACCATCTTCCTCGCCGGCCCGCCGCTGGTGaaa                                                           >  1:1200810/1‑150 (MQ=255)
                                                                          gCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTGCGCCAGCAGGCGACCATCTTCCTCGCCGGCCCGCCGCTGGTGaaa                                                           <  1:993388/150‑1 (MQ=255)
                                                                            cAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTGCGCCAGCAGGCGACCATCTTCCTCGCCGGCCCGCCGCTGGTGAAAgc                                                         >  2:675798/1‑150 (MQ=255)
                                                                             aaCATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCTGCAGTAGCTCAAGCAGTGCTTACAGTGCATCAGCAGGCGACCATCTTCCTCGCCGTCCCGCCGCTCGTGAAAgcc                                                        >  1:878559/1‑150 (MQ=255)
                                                                                    gcgcgcAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTGCGCCAGCAGGCGACCATCTTCCTCGCCGGCCCGCCGCTGGTGAAAGCCGCCACCg                                                 <  1:634681/150‑1 (MQ=255)
                                                                                            ggCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTGCGCCAGCAGGCGACCATCTTCCTCGCCGGCCCGCCGCTGGTGAAAGCCGCCACCGGCGAAgtg                                         <  1:675798/150‑1 (MQ=255)
                                                                                             gCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTGCGCCAGCAGGCGACCATCTTCCTCGCCGGCCCGCCGCTGGTGAAAGCCGCCACCGGCGAAgtgg                                        <  1:650840/150‑1 (MQ=255)
                                                                                              cATCCCGCAGATCGCCGTGGTGTTGGGCTCGTGCACCGCTGGCGGGGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTGCGCCAGCAGGCGACCATCTTCCTCGCCGGCCCGCCGCTGGTGAAAGCCGCCACCGGCGAAgtggt                                       <  2:735385/150‑1 (MQ=255)
                                                                                               aTCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTGCGCCAGCAGGCGACCATCTTCCTCGCCGGCCCGCCGCTGGTGAAAGCCGCCACCGGCGAAgtggtg                                      <  2:1419205/150‑1 (MQ=255)
                                                                                                 cccGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATCGTGCGCCAGCAGGCGAACATCGGCGTCACCTTCCTGTCTCTGGTGAAAGCCGCCACCGGCGAAGTGGTGAg                                    <  1:603592/150‑1 (MQ=255)
                                                                                                    gCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTGCGCCAGCAGGCGACCATCTTCCTCGCCGGCCCGCCGCTGGTGAAAGCCGCCACCGGCGAAGTGGTGAgcgc                                 >  2:497270/1‑150 (MQ=255)
                                                                                                       gATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTGCGCCAGCAGGCGACCATCTTCCTCGCCGGCCCGCCGCTGGTGAAAGCCGCCACCGGCGAAGTGGTGAGCGCCGa                              <  1:516903/150‑1 (MQ=255)
                                                                                                         tCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTGCGCCAGCAGGCGACCATCTTCCTCGCCGGCCCGCCGCTGGTGAAAGCCGCCACCGGCGAAGTGGTGAGCGCCGAAg                            >  2:1256299/1‑150 (MQ=255)
                                                                                                           gCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTGCGCCAGCAGGCGACCATCTTCCTCGCCGGCCCGCCGCTGGTGAAAGCCGCCACCGGCGAAGTGGTGAGCGCCGAAGAc                          >  1:1369055/1‑150 (MQ=255)
                                                                                                                          tCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTGCGCCAGCAGGCGACCATCTTCCTCGCCGGCCCGCCGCTGGTGAAAGCCGCCACCGGCGAAGTGGTGAGCGCCGAAGACCTCGGCGGCGCCGAt           <  1:1256299/150‑1 (MQ=255)
                                                                                                                                aCCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTGCGCCAGCAGGCGACCATCTTCCTCGCCGGCCCGCCGCTGGTGAAAGCCGCCACCGGCGAAGTGGTGAGCGCCGAAGACCTCGGCGGCGCCGATGTGCAt     >  2:532286/1‑150 (MQ=255)
                                                                                                                                   gCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTGCGCCAGCAGGCGACCATCTTCCTCGCCGGCCCGCCGCTGGTGAAAGCCGCCACCGGCGAAGTGGTGAGCGCCGAAGACCTCGGCGGCGCCGATGTGCATTGc  <  2:878559/150‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GCGGCGCCAACTTGCCCCGCCAGGACGAAGTGTTCCCCGACCGCGAGCATTTCGGGCGCATTTTCTTCAACCAGGCCAACATGAGCGCGCAAGGCATCCCGCAGATCGCCGTGGTGATGGGCTCGTGCACCGCTGGCGGCGCGTACGTGCCGGCCATGGCCGACGAAGCGATCATGGTGCGCCAGCAGGCGACCATCTTCCTCGCCGGCCCGCCGCTGGTGAAAGCCGCCACCGGCGAAGTGGTGAGCGCCGAAGACCTCGGCGGCGCCGATGTGCATTGC  >  NC_002947/4589801‑4590081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑