Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,598,544 C→G 6.6% A437A (GCG→GCC)  PP_4071 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,598,5440CG 6.6% 292.5 / 8.9 91A437A (GCG→GCC) PP_4071membrane protein
Reads supporting (aligned to +/- strand):  ref base C (43/42);  new base G (3/3);  total (46/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

GCGATGGCGACGCAGAAGGCCAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCACCAGCGCCGGGGTGGTGAAACGG  >  NC_002947/4598395‑4598691
                                                                                                                                                     |                                                                                                                                                   
gcgatggcgaCGCAGAAGGCCAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAAc                                                                                                                                                     <  2:506356/150‑1 (MQ=255)
  gatggcgaCGCAGAAGGCCAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGc                                                                                                                                                   >  1:814983/1‑150 (MQ=255)
       cgaCGCAGAAGGCCAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTgg                                                                                                                                              >  2:144769/1‑150 (MQ=255)
        gaCGCAGAAGGCCAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGc                                                                                                                                             <  2:123754/150‑1 (MQ=255)
           gCAGAAGGCCAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTg                                                                                                                                          >  2:52546/1‑150 (MQ=255)
             aGAAGGCCAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTgtg                                                                                                                                        >  2:1233242/1‑148 (MQ=255)
              gAAGGCCAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCgg                                                                                                                                       >  1:1120603/1‑150 (MQ=255)
              gAAGGCCAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCgg                                                                                                                                       >  1:610005/1‑150 (MQ=255)
               aaGGCCAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGc                                                                                                                                      <  2:1367261/150‑1 (MQ=255)
                 ggCCAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCtt                                                                                                                                    <  2:610005/150‑1 (MQ=255)
                 ggCCAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCtt                                                                                                                                    <  1:144769/150‑1 (MQ=255)
                    cAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAAGGCGTTGGCCtac                                                                                                                                         >  2:1090912/1‑140 (MQ=255)
                    cAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAAGGCGTTGGCCtac                                                                                                                                         >  2:1090871/1‑140 (MQ=255)
                    cAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAAGGCGTTGGCCtac                                                                                                                                         <  1:1090912/142‑3 (MQ=255)
                    cAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAAGGCGTTGGCCtac                                                                                                                                         <  1:1090871/142‑3 (MQ=255)
                    cAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGcc                                                                                                                                 >  2:1012687/1‑150 (MQ=255)
                     aGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGccc                                                                                                                                >  1:1400722/1‑150 (MQ=255)
                         cGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCaa                                                                                                                            <  1:1325724/150‑1 (MQ=255)
                          gCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAg                                                                                                                           >  2:464889/1‑150 (MQ=255)
                             tACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCaa                                                                                                                        >  2:1217929/1‑150 (MQ=255)
                                agaagaACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTg                                                                                                                                               <  1:576203/124‑1 (MQ=255)
                                agaagaACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTg                                                                                                                                               >  2:576203/1‑124 (MQ=255)
                                    gaaCCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAgg                                                                                                                 >  1:923586/1‑150 (MQ=255)
                                    gaaCCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAgg                                                                                                                 >  1:1190241/1‑150 (MQ=255)
                                    gaaCCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAgg                                                                                                                 <  1:254990/150‑1 (MQ=255)
                                        ccaccctcAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTgg                                                                                                             >  1:480611/1‑150 (MQ=255)
                                          acccacAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTc                                                                                                           <  2:923586/150‑1 (MQ=255)
                                           cccacAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCt                                                                                                          <  1:52546/150‑1 (MQ=255)
                                            ccacAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAAc                                                                                                                                                     <  2:1410387/106‑1 (MQ=255)
                                            ccacAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAAc                                                                                                                                                     >  1:1410387/1‑106 (MQ=255)
                                            ccacAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCtt                                                                                                         <  1:1217929/150‑1 (MQ=255)
                                             cacaGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACgg                                                                                                                     <  2:1213238/137‑1 (MQ=255)
                                             cacaGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACgg                                                                                                                     >  1:1213238/1‑137 (MQ=255)
                                              acaGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCGAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGt                                                                                                       >  1:680859/1‑150 (MQ=255)
                                                    gTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCt                                                                                                 <  1:456760/150‑1 (MQ=255)
                                                      tGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGTAATGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTgc                                                                                               <  1:434841/150‑1 (MQ=255)
                                                        gcgcTTGTGCCGCGCGACAATGACGGTATCGCCGGCAAGGCCTGGCTCTTTGTAGATGACCCCCCCAAAGGCATGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCTGCTTGCCCTCAAGCAACGGGAGTCTGGTCTTGTAAGGCTtcgc                                                                                             >  2:448679/1‑150 (MQ=255)
                                                        gcgcTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTgcgc                                                                                             <  1:920897/150‑1 (MQ=255)
                                                         cgcTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGcgcc                                                                                            <  1:1012687/150‑1 (MQ=255)
                                                                   gAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGTGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGa                                                                                  <  1:1233242/150‑1 (MQ=255)
                                                                      ccACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACaa                                                                               <  2:480611/150‑1 (MQ=255)
                                                                        aCCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAg                                                                             >  2:1238490/1‑150 (MQ=255)
                                                                        aCCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAg                                                                             >  1:999912/1‑150 (MQ=255)
                                                                         cccTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAgg                                                                            >  1:1004262/1‑150 (MQ=255)
                                                                          ccTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGc                                                                           >  2:1003909/1‑150 (MQ=255)
                                                                          ccTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGc                                                                           >  2:664820/1‑150 (MQ=255)
                                                                            tGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTACGGTAAGGCTGCGCCGCCTCACGGACAAAGGCGt                                                                         <  1:1182292/150‑1 (MQ=255)
                                                                              aCGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGGCCCGCCCAAAGGCCTATTAAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTCAGGATCCGCCGCATCACGGACAAAGGTGTTg                                                                       <  2:799624/150‑1 (MQ=255)
                                                                              aCGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTg                                                                       <  1:464889/150‑1 (MQ=255)
                                                                                 ttATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTgcgc                                                                    >  1:684258/1‑150 (MQ=255)
                                                                                 ttATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTgcgc                                                                    >  1:773732/1‑150 (MQ=255)
                                                                                  tctagCCGGCAAGGGCGGGGTCTTTTTAGATGACCAGCCCAAAGGCCTGTTGAATGACGTAGGACAACGCTTTGGCCTGCGGCTTGCCCTCAAGCCACGGGAAGCTGGTCTTGTAAGGCTGCGCCCCCGCCCGGACAAAAGCGTCTCGCa                                                                   >  2:270681/5‑150 (MQ=255)
                                                                                   aTCGCCGGCAAGGCCGGCGTCTTTGTAGATGACCCGTCCAAAGGTCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACTAAGGCGTTGCGCAc                                                                  <  2:230793/150‑1 (MQ=255)
                                                                                    tCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACg                                                                 >  2:57167/1‑150 (MQ=255)
                                                                                        cGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCAc                                                             >  2:1227298/1‑150 (MQ=255)
                                                                                           cAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGCAGGCCAAGGCGTTGGCCtac                                                                                                                                         <  1:283034/71‑3 (MQ=38)
                                                                                           cAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGCAGGCCAAGGCGTTGGCCtac                                                                                                                                         >  2:283034/1‑69 (MQ=37)
                                                                                                ccggccTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGt                                                     <  2:624412/150‑1 (MQ=255)
                                                                                                    ccTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGACCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGa                                                 <  2:1255054/150‑1 (MQ=255)
                                                                                                      tctTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACt                                               >  2:53026/1‑150 (MQ=255)
                                                                                                        tttGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGcc                                                                                                                                 <  1:110090/66‑1 (MQ=255)
                                                                                                        tttGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGcc                                                                                                                                 >  2:110090/1‑66 (MQ=255)
                                                                                                        tttGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGACTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTga                                             <  2:814983/150‑1 (MQ=255)
                                                                                                        tttGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGATACGGGAGGCTGGTCTTGTAAGGCTGCGCCGTCCCTCGGACAAAGGCGTTGCGCACGTCACCGTGCTGTCCGACTga                                             >  1:674136/1‑150 (MQ=255)
                                                                                                         ttGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTgac                                            <  1:1238490/150‑1 (MQ=255)
                                                                                                         ttGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTgac                                            <  1:57167/150‑1 (MQ=255)
                                                                                                          tGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTgc                                                                      >  2:1423073/1‑123 (MQ=255)
                                                                                                          tGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTCgc                                                                      <  1:1423073/123‑1 (MQ=255)
                                                                                                           gTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTgacga                                          <  1:1227298/150‑1 (MQ=255)
                                                                                                           gTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTgacga                                          <  2:457983/150‑1 (MQ=255)
                                                                                                           gTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTAGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTgacga                                          >  2:657171/1‑150 (MQ=255)
                                                                                                            tAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTAGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTgacgac                                         <  1:657171/150‑1 (MQ=255)
                                                                                                               aTGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCaa                                      <  1:53026/150‑1 (MQ=255)
                                                                                                                   cccGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATaca                                  >  1:273033/1‑150 (MQ=255)
                                                                                                                      gCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCaa                                                                                                                            <  1:653336/57‑1 (MQ=255)
                                                                                                                      gCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCaa                                                                                                                            >  2:653336/1‑57 (MQ=255)
                                                                                                                            aGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCa                         <  2:1120603/150‑1 (MQ=255)
                                                                                                                                 tGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCACCAgc                    >  1:1137042/1‑150 (MQ=255)
                                                                                                                                   ttGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCACCAgcgc                  >  1:757879/1‑150 (MQ=255)
                                                                                                                                      aatgaaGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCACGCAACGGGAGGCTGGTCGTGTAAGGCTGCGACACACCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCACCAGCGCCgg               <  1:319962/150‑1 (MQ=255)
                                                                                                                                      aatgaaGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGt                                                                <  2:938234/101‑1 (MQ=255)
                                                                                                                                      aatgaaGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGt                                                                >  1:938234/1‑101 (MQ=255)
                                                                                                                                      aatgaaGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCACCAGCGCCgg               >  2:45483/1‑150 (MQ=255)
                                                                                                                                      aatgaaGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCACCAGCGCCgg               >  1:466637/1‑150 (MQ=255)
                                                                                                                                      aatgaaGTAGGCCAACGACGTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCACCAGCGCCgg               >  1:49151/1‑150 (MQ=255)
                                                                                                                                           aGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCACCAGCGCCGGggtgg          <  2:999912/150‑1 (MQ=255)
                                                                                                                                           aGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCACCAGCGCCGGggtgg          <  2:1190241/150‑1 (MQ=255)
                                                                                                                                            gTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCACCAGCGCCGGggtggt         <  1:664820/150‑1 (MQ=255)
                                                                                                                                                  cAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTgacgac                                         >  1:1288876/1‑112 (MQ=255)
                                                                                                                                                  cAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTgacgac                                         <  2:1288876/112‑1 (MQ=255)
                                                                                                                                                  cAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCACCAGCGCCGGggt            <  2:454037/141‑1 (MQ=255)
                                                                                                                                                  cAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCACCAGCGCCGGggt            >  1:454037/1‑141 (MQ=255)
                                                                                                                                                  cAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCACCAGCGCCGGGGTGGTGAAACg   <  2:273033/150‑1 (MQ=255)
                                                                                                                                                   aaCGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCACCAGCGCCGGGGTGGTGAAACgg  >  1:826412/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GCGATGGCGACGCAGAAGGCCAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCACCAGCGCCGGGGTGGTGAAACGG  >  NC_002947/4598395‑4598691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑