Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,598,547 C→G 6.8% K436N (AAG→AAC)  PP_4071 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,598,5470CG 6.8% 280.9 / 8.6 90K436N (AAG→AAC) PP_4071membrane protein
Reads supporting (aligned to +/- strand):  ref base C (41/42);  new base G (3/3);  total (45/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

CGACGCAGAAGGCCAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCACCAGCGCCGGGGTGGTGAAACGGTCAC  >  NC_002947/4598402‑4598695
                                                                                                                                                 |                                                                                                                                                    
cgaCGCAGAAGGCCAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTgg                                                                                                                                                  >  2:144769/1‑150 (MQ=255)
 gaCGCAGAAGGCCAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGc                                                                                                                                                 <  2:123754/150‑1 (MQ=255)
    gCAGAAGGCCAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTg                                                                                                                                              >  2:52546/1‑150 (MQ=255)
      aGAAGGCCAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTgtg                                                                                                                                            >  2:1233242/1‑148 (MQ=255)
       gAAGGCCAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCgg                                                                                                                                           >  1:610005/1‑150 (MQ=255)
       gAAGGCCAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCgg                                                                                                                                           >  1:1120603/1‑150 (MQ=255)
        aaGGCCAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGc                                                                                                                                          <  2:1367261/150‑1 (MQ=255)
          ggCCAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCtt                                                                                                                                        <  2:610005/150‑1 (MQ=255)
          ggCCAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCtt                                                                                                                                        <  1:144769/150‑1 (MQ=255)
             cAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAAGGCGTTGGCCtac                                                                                                                                             >  2:1090912/1‑140 (MQ=255)
             cAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAAGGCGTTGGCCtac                                                                                                                                             >  2:1090871/1‑140 (MQ=255)
             cAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAAGGCGTTGGCCtac                                                                                                                                             <  1:1090871/142‑3 (MQ=255)
             cAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAAGGCGTTGGCCtac                                                                                                                                             <  1:1090912/142‑3 (MQ=255)
             cAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGcc                                                                                                                                     >  2:1012687/1‑150 (MQ=255)
              aGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGccc                                                                                                                                    >  1:1400722/1‑150 (MQ=255)
                  cGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCaa                                                                                                                                <  1:1325724/150‑1 (MQ=255)
                   gCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAg                                                                                                                               >  2:464889/1‑150 (MQ=255)
                      tACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCaa                                                                                                                            >  2:1217929/1‑150 (MQ=255)
                         agaagaACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTg                                                                                                                                                   <  1:576203/124‑1 (MQ=255)
                         agaagaACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTg                                                                                                                                                   >  2:576203/1‑124 (MQ=255)
                             gaaCCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAgg                                                                                                                     <  1:254990/150‑1 (MQ=255)
                             gaaCCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAgg                                                                                                                     >  1:923586/1‑150 (MQ=255)
                             gaaCCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAgg                                                                                                                     >  1:1190241/1‑150 (MQ=255)
                                 ccaccctcAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTgg                                                                                                                 >  1:480611/1‑150 (MQ=255)
                                   acccacAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTc                                                                                                               <  2:923586/150‑1 (MQ=255)
                                    cccacAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCt                                                                                                              <  1:52546/150‑1 (MQ=255)
                                     ccacAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCtt                                                                                                             <  1:1217929/150‑1 (MQ=255)
                                      cacaGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACgg                                                                                                                         <  2:1213238/137‑1 (MQ=255)
                                      cacaGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACgg                                                                                                                         >  1:1213238/1‑137 (MQ=255)
                                       acaGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCGAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGt                                                                                                           >  1:680859/1‑150 (MQ=255)
                                             gTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCt                                                                                                     <  1:456760/150‑1 (MQ=255)
                                               tGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGTAATGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTgc                                                                                                   <  1:434841/150‑1 (MQ=255)
                                                 gcgcTTGTGCCGCGCGACAATGACGGTATCGCCGGCAAGGCCTGGCTCTTTGTAGATGACCCCCCCAAAGGCATGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCTGCTTGCCCTCAAGCAACGGGAGTCTGGTCTTGTAAGGCTtcgc                                                                                                 >  2:448679/1‑150 (MQ=255)
                                                 gcgcTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTgcgc                                                                                                 <  1:920897/150‑1 (MQ=255)
                                                  cgcTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGcgcc                                                                                                <  1:1012687/150‑1 (MQ=255)
                                                            gAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGTGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGa                                                                                      <  1:1233242/150‑1 (MQ=255)
                                                               ccACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACaa                                                                                   <  2:480611/150‑1 (MQ=255)
                                                                 aCCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAg                                                                                 >  2:1238490/1‑150 (MQ=255)
                                                                 aCCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAg                                                                                 >  1:999912/1‑150 (MQ=255)
                                                                  cccTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAgg                                                                                >  1:1004262/1‑150 (MQ=255)
                                                                   ccTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGc                                                                               >  2:664820/1‑150 (MQ=255)
                                                                   ccTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGc                                                                               >  2:1003909/1‑150 (MQ=255)
                                                                     tGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTACGGTAAGGCTGCGCCGCCTCACGGACAAAGGCGt                                                                             <  1:1182292/150‑1 (MQ=255)
                                                                       aCGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGGCCCGCCCAAAGGCCTATTAAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTCAGGATCCGCCGCATCACGGACAAAGGTGTTg                                                                           <  2:799624/150‑1 (MQ=255)
                                                                       aCGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTg                                                                           <  1:464889/150‑1 (MQ=255)
                                                                          ttATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTgcgc                                                                        >  1:684258/1‑150 (MQ=255)
                                                                          ttATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTgcgc                                                                        >  1:773732/1‑150 (MQ=255)
                                                                           tctagCCGGCAAGGGCGGGGTCTTTTTAGATGACCAGCCCAAAGGCCTGTTGAATGACGTAGGACAACGCTTTGGCCTGCGGCTTGCCCTCAAGCCACGGGAAGCTGGTCTTGTAAGGCTGCGCCCCCGCCCGGACAAAAGCGTCTCGCa                                                                       >  2:270681/5‑150 (MQ=255)
                                                                            aTCGCCGGCAAGGCCGGCGTCTTTGTAGATGACCCGTCCAAAGGTCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACTAAGGCGTTGCGCAc                                                                      <  2:230793/150‑1 (MQ=255)
                                                                             tCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACg                                                                     >  2:57167/1‑150 (MQ=255)
                                                                                 cGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCAc                                                                 >  2:1227298/1‑150 (MQ=255)
                                                                                    cAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGCAGGCCAAGGCGTTGGCCtac                                                                                                                                             >  2:283034/1‑69 (MQ=37)
                                                                                    cAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGCAGGCCAAGGCGTTGGCCtac                                                                                                                                             <  1:283034/71‑3 (MQ=38)
                                                                                         ccggccTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGt                                                         <  2:624412/150‑1 (MQ=255)
                                                                                             ccTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGACCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGa                                                     <  2:1255054/150‑1 (MQ=255)
                                                                                               tctTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACt                                                   >  2:53026/1‑150 (MQ=255)
                                                                                                 tttGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGcc                                                                                                                                     >  2:110090/1‑66 (MQ=255)
                                                                                                 tttGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGcc                                                                                                                                     <  1:110090/66‑1 (MQ=255)
                                                                                                 tttGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGACTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTga                                                 <  2:814983/150‑1 (MQ=255)
                                                                                                 tttGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGATACGGGAGGCTGGTCTTGTAAGGCTGCGCCGTCCCTCGGACAAAGGCGTTGCGCACGTCACCGTGCTGTCCGACTga                                                 >  1:674136/1‑150 (MQ=255)
                                                                                                  ttGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTgac                                                <  1:1238490/150‑1 (MQ=255)
                                                                                                  ttGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTgac                                                <  1:57167/150‑1 (MQ=255)
                                                                                                   tGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTgc                                                                          >  2:1423073/1‑123 (MQ=255)
                                                                                                   tGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTCgc                                                                          <  1:1423073/123‑1 (MQ=255)
                                                                                                    gTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTgacga                                              <  2:457983/150‑1 (MQ=255)
                                                                                                    gTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTgacga                                              <  1:1227298/150‑1 (MQ=255)
                                                                                                    gTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTAGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTgacga                                              >  2:657171/1‑150 (MQ=255)
                                                                                                     tAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTAGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTgacgac                                             <  1:657171/150‑1 (MQ=255)
                                                                                                        aTGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCaa                                          <  1:53026/150‑1 (MQ=255)
                                                                                                            cccGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATaca                                      >  1:273033/1‑150 (MQ=255)
                                                                                                               gCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCaa                                                                                                                                >  2:653336/1‑57 (MQ=255)
                                                                                                               gCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCaa                                                                                                                                <  1:653336/57‑1 (MQ=255)
                                                                                                                     aGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCa                             <  2:1120603/150‑1 (MQ=255)
                                                                                                                          tGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCACCAgc                        >  1:1137042/1‑150 (MQ=255)
                                                                                                                            ttGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCACCAgcgc                      >  1:757879/1‑150 (MQ=255)
                                                                                                                               aatgaaGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCACGCAACGGGAGGCTGGTCGTGTAAGGCTGCGACACACCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCACCAGCGCCgg                   <  1:319962/150‑1 (MQ=255)
                                                                                                                               aatgaaGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGt                                                                    >  1:938234/1‑101 (MQ=255)
                                                                                                                               aatgaaGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGt                                                                    <  2:938234/101‑1 (MQ=255)
                                                                                                                               aatgaaGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCACCAGCGCCgg                   >  1:466637/1‑150 (MQ=255)
                                                                                                                               aatgaaGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCACCAGCGCCgg                   >  2:45483/1‑150 (MQ=255)
                                                                                                                               aatgaaGTAGGCCAACGACGTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCACCAGCGCCgg                   >  1:49151/1‑150 (MQ=255)
                                                                                                                                    aGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCACCAGCGCCGGggtgg              <  2:999912/150‑1 (MQ=255)
                                                                                                                                    aGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCACCAGCGCCGGggtgg              <  2:1190241/150‑1 (MQ=255)
                                                                                                                                     gTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCACCAGCGCCGGggtggt             <  1:664820/150‑1 (MQ=255)
                                                                                                                                           cAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTgacgac                                             >  1:1288876/1‑112 (MQ=255)
                                                                                                                                           cAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTgacgac                                             <  2:1288876/112‑1 (MQ=255)
                                                                                                                                           cAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCACCAGCGCCGGggt                <  2:454037/141‑1 (MQ=255)
                                                                                                                                           cAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCACCAGCGCCGGggt                >  1:454037/1‑141 (MQ=255)
                                                                                                                                           cAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCACCAGCGCCGGGGTGGTGAAACg       <  2:273033/150‑1 (MQ=255)
                                                                                                                                            aaCGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCACCAGCGCCGGGGTGGTGAAACgg      >  1:826412/1‑150 (MQ=255)
                                                                                                                                                ccTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCACCAGCGCCGGGGTGGTGAAACGGTCAc  >  2:197466/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CGACGCAGAAGGCCAGAACGCCTACAGAAGAACCCACCCACAGCAGTTGGCGCTTGCGCCGAGCCACCCTGACGTTATCGCCGGCAAGGCCGGCCTCTTTGTAGATGACCCGCCCAAAGGCCTGTTGAATGAAGTAGGCCAACGCCTTGGCCTGCGGCTTGCCCTCAAGCAACGGGAGGCTGGTCTTGTAAGGCTGCGCCGCCTCACGGACAAAGGCGTTGCGCACGTCACCGTGCTGTACGACTGACGACCAATACACACCGCGCACCAGCGCCGGGGTGGTGAAACGGTCAC  >  NC_002947/4598402‑4598695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑