Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,614,851 G→A 7.8% E392E (GAG→GAA)  PP_4085 → rhs family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,614,8510GA 7.8% 141.5 / 2.5 51E392E (GAG→GAA) PP_4085rhs family protein
Reads supporting (aligned to +/- strand):  ref base G (26/21);  new base A (2/2);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

GTGGAGCTGGCCCGCGTCCCACATCCTCAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTCGACCTGCTGTACGACGAACAAGGGCGCC  >  NC_002947/4614705‑4614999
                                                                                                                                                  |                                                                                                                                                    
gTGGAGCTGGCCCGCGTCCCACATCCTCAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGAc                                                                                                                                                   >  1:400222/1‑150 (MQ=255)
      cTGGCCCGCGTCCCACATCCTCAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAg                                                                                                                                             >  1:1179000/1‑150 (MQ=255)
         gCCCGCGTCCCACATCCTCAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGAcc                                                                                                                                          >  2:1301318/1‑150 (MQ=255)
           ccGCGTCCCACATCCTCAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGTGGACGGCCAGACCGt                                                                                                                                        >  1:504195/1‑150 (MQ=255)
            cgcgTCCCACATCCTCAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGtt                                                                                                                                       >  1:1079193/1‑150 (MQ=255)
              cgTCCCACATCCTCAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGTGGACGGCCAGACCGTTGt                                                                                                                                     <  2:504195/150‑1 (MQ=255)
                  ccACATCCTCAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGTCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGaa                                                                                                                                 <  2:343933/150‑1 (MQ=255)
                  ccACATCCTCAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGaa                                                                                                                                 <  2:1079193/150‑1 (MQ=255)
                   cacaTCCTCAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGTCTGGCCGTCCTCCAGCTGGAAGAAGGc                                                                                                                                                <  2:936548/134‑1 (MQ=38)
                   cacaTCCTCAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGTCTGGCCGTCCTCCAGCTGGAAGAAGGc                                                                                                                                                >  1:936548/1‑134 (MQ=38)
                   cacaTCCTCAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAg                                                                                                                                >  1:1089950/1‑150 (MQ=255)
                          tCAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCaa                                                                                                                         >  1:450327/1‑150 (MQ=255)
                               gCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTTGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAg                                                                                                                    <  1:616012/150‑1 (MQ=255)
                                 ggTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAggg                                                                                                                  >  2:391671/1‑150 (MQ=255)
                                  gTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGTGc                                                                                                                 <  2:386039/150‑1 (MQ=255)
                                      gCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGATGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTa                                                                                                             >  2:1052421/1‑150 (MQ=255)
                                          tgGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCt                                                                                                                <  2:344388/143‑1 (MQ=255)
                                          tgGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCt                                                                                                                >  1:344388/1‑143 (MQ=255)
                                          tgGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCgg                                                                                                         <  2:400222/150‑1 (MQ=255)
                                                  cATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGa                                                                                                 <  1:965631/150‑1 (MQ=255)
                                                      gACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATAcc                                                                                             >  2:1240287/1‑150 (MQ=255)
                                                         aaCGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGAc                                                                                          <  1:391671/150‑1 (MQ=255)
                                                                   aCCGACTGGACCTCAGCCGCATCAGAGGCGGTGATGTCTTCATCCATGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATc                                                                                <  2:434662/150‑1 (MQ=255)
                                                                   aCCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATAc                                                                                              <  1:850747/136‑1 (MQ=255)
                                                                   aCCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATAc                                                                                              >  2:850747/1‑136 (MQ=255)
                                                                    ccGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACgg                                                                                                                                                 <  1:777794/84‑1 (MQ=255)
                                                                    ccGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACgg                                                                                                                                                 >  2:777794/1‑84 (MQ=255)
                                                                      gACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGa                                                                             >  1:1869/1‑150 (MQ=255)
                                                                      gACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTATTCCAGATGGAGGACGGCCAGACCGTTGTCCAAGATATTAATTAAGGGCATTATCGGGGCTTCGATACCGACCCGCTTAATCCGa                                                                             >  2:1142793/1‑150 (MQ=255)
                                                                             aCTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTc                                                                      >  2:1297241/1‑150 (MQ=255)
                                                                                 ggCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTAt                                                                                                            >  1:1000116/1‑108 (MQ=255)
                                                                                 ggCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTAt                                                                                                            <  2:1000116/108‑1 (MQ=255)
                                                                                  gccgccTCAGTGCCGGTAATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATAACTGCAGGGCTTTCGCGGACGCGCTGAACCGGTCTGCTGAAGCCGAAACGCTCACGGc                                                                 >  2:726964/1‑150 (MQ=255)
                                                                                   ccgccTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGCACGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCt                                                                <  1:726964/150‑1 (MQ=255)
                                                                                      ccTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGAt                                                             <  1:1301318/150‑1 (MQ=255)
                                                                                        tCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCa                                                           <  2:1179000/150‑1 (MQ=255)
                                                                                         cAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAg                                                          <  1:804634/150‑1 (MQ=255)
                                                                                            tGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCt                                                       >  2:1388633/1‑150 (MQ=255)
                                                                                                        cTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAg                                                                                                                    <  2:756173/77‑1 (MQ=255)
                                                                                                        cTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAg                                                                                                                    >  1:756173/1‑77 (MQ=255)
                                                                                                        cTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCaa                                           >  1:912897/1‑150 (MQ=255)
                                                                                                        cTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCaa                                           >  2:803349/1‑150 (MQ=255)
                                                                                                                        gACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTCGAc                           <  2:1299173/150‑1 (MQ=255)
                                                                                                                         aCGGCTTGGCCTCATTCACGCTGGAGGACGGCCAGACCGCTGTCGAAGATGTCAATGAAGGGCGTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTCGAcc                          >  2:1086873/1‑150 (MQ=255)
                                                                                                                             tctGGCCGTCCTCCAGCTGGAAGAAGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTCGACctgct                      >  1:670469/3‑150 (MQ=255)
                                                                                                                              ttGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGaaa                                                                           >  2:1116996/1‑96 (MQ=255)
                                                                                                                              ttGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGaaa                                                                           <  1:1116996/96‑1 (MQ=255)
                                                                                                                                  ccTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCg                                                                                            <  2:472849/75‑1 (MQ=255)
                                                                                                                                  ccTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCg                                                                                            >  1:472849/1‑75 (MQ=255)
                                                                                                                                           aGCTGGAGGACCGCCCGACCGGTTTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTCGACCTGCTGTACGACGAACAAg        >  2:1060029/1‑150 (MQ=255)
                                                                                                                                           aGCTGGAAGAAGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTCGACCTGCTGTACGACGAACAAg        <  2:670469/150‑1 (MQ=255)
                                                                                                                                                 aggaCGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTCGACCTGCTGTACGACGAACAAGGGCGcc  <  1:1052421/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GTGGAGCTGGCCCGCGTCCCACATCCTCAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTCGACCTGCTGTACGACGAACAAGGGCGCC  >  NC_002947/4614705‑4614999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑