Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,705,459	T→A	31.3%	intergenic (‑151/+26)	kdpF ← / ← PP_4162	potassium‑transporting ATPase subunit F/membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,705,459	0	T	A	31.3%	61.3 / 40.1	48	intergenic (‑151/+26)	kdpF/PP_4162	potassium‑transporting ATPase subunit F/membrane protein
Reads supporting (aligned to +/- strand):  ref base T (16/17);  new base A (8/7);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01

TCTCCCCGTGTTACGGCTTGGGCCGCTTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAAC  >  NC_002947/4705316‑4705608                                                                                                                                                |                                                                                                                                                      tctcCCCGTGTTACGGCTTGGGCCGCTTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGcccc                                                                                                                                                 >  2:626577/1‑149 (MQ=255)     cggatcttAGGGCTTGGGCCGCTTTGGGGAAATGGTCGGGGGGAGAGGGGAAAGGGGGCGAGATGGGGGGGTGGGGGGGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCCATTCGCGGGCAAGCCCGCGaa                                                                                                                                             <  2:420232/144‑1 (MQ=255)     cccGTGTTACGGCTTGGGCCGCTTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCCTTTCGCGGGCAAGCCCGCGaa                                                                                                                                             >  1:600947/1‑150 (MQ=255)      ccGTGTTCCGGCTGGGGCCGCTTTGGGGAAATTGTCCGAGGGAGGGGCTAAAAGGGGCGAGATGGGGGGGTGGGGCTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGaaa                                                                                                                                            <  1:99281/150‑1 (MQ=255)         tgtTACGGCTTGGGCCGCTTTGGGGAATTTGCCCGAGGGAGAGGCGTAAAGGGGCGAGAGGGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCCTTTCGCGGGCAAGCCCGCGaagagg                                                                                                                                         <  1:80496/150‑5 (MQ=37)           ttAGGGCGTGGGCGGTGTTGGGGAAATTGCCCGAAGGAGGGGTGTAAGGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCTTAAAAATTAGCTTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGcc                                                                                                                                       <  2:275727/150‑1 (MQ=255)             cggccTTGGGCCGTTTGGGGGAAATTGTCGGAGGAAGAGGCGAAAAGGGGCGAGAGGGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGt                                                                                                                                     <  1:442719/146‑1 (MQ=255)                    gggCGGCTGGGGGGACGTTGGCAGGGGGGGCGGGGTAGAGGGGGGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGc                                                                                                                              <  1:1118083/150‑1 (MQ=255)                     ggcggcTTGGGGGAAATTGGCCGGGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCCTTTCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGca                                                                                                                             <  2:600947/150‑2 (MQ=25)                     ggCCGGTTGGGGGAAATTGTCGGGGGGGGAGGGGAAAAGGGGCGAGAGGGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCt                                                                                                                             <  2:1336674/150‑1 (MQ=255)                      gCCGCTTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGGTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCtt                                                                                                                            >  1:989490/1‑150 (MQ=255)                              ggagAAACTGTGCGCAGGAGTGGGGTATAGGGGTGTGTTCGGGGTGGGGAGGTCGGTATAAAGAATGCGTAAACATCCGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATtgttgt                                                                                                                    <  1:103304/147‑1 (MQ=255)                                 gAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTAttgagatcgg                                                                                                                 >  1:1045948/1‑143 (MQ=255)                                        tCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGgctgct                                                                                                          >  1:188440/1‑150 (MQ=255)                                        tCCGAGGGAGAGGAGTAAAGGGGCGACGGCGGGGGGTGGGGGTGGGAATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGgctgct                                                                                                          <  1:551396/150‑1 (MQ=255)                                           gAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCCTTTCGCGGGCAAGCCCGCGAAGAGGCCGTTACAg                                                                                                                                <  2:963133/125‑1 (MQ=25)                                           gAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCCTTTCGCGGGCAAGCCCGCGAAGAGGCCGTTACAg                                                                                                                                >  1:963133/1‑125 (MQ=25)                                             gggAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGATGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCCTATTGTTGTGGCGGCTGCTGcgcc                                                                                                     >  2:675948/1‑150 (MQ=255)                                                                      ggggggTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCcagcagca                                                                            <  2:188440/150‑1 (MQ=255)                                                                      ggggggTGGGGGGGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCcagcagca                                                                            <  2:737451/150‑1 (MQ=255)                                                                        ggggggggggggTGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAgg                                                                          <  1:1064434/150‑1 (MQ=255)                                                                               gggtgggtATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGc                                                                   <  2:1063553/150‑1 (MQ=255)                                                                                        taAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAgg                                                                          >  1:590573/1‑134 (MQ=255)                                                                                        taAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGCCGGCTGCTGCTCCGCGTTACGGCTCCAGTCCAGCAGCAgg                                                                          <  2:590573/134‑1 (MQ=255)                                                                                              aTGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGgct                                                                                                             >  1:870097/1‑93 (MQ=255)                                                                                              aTGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGgct                                                                                                             <  2:870097/93‑1 (MQ=255)                                                                                                                      cTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGGCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGa                            >  2:762257/1‑150 (MQ=255)                                                                                                                          cTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGcc                        >  1:1217710/1‑150 (MQ=255)                                                                                                                              aaCGGCCCTTTCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTCACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAg                    <  2:813802/150‑1 (MQ=38)                                                                                                                                 ggCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATAGTTGTGGCGGCTGCCGCACCGGATTACGGCTCCAATCCAGCAGCACGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATCAAGCCAAAGgcc                 >  2:520187/1‑149 (MQ=255)                                                                                                                                  gccctttCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGgcaa                >  2:443355/6‑150 (MQ=38)                                                                                                                                  gccctttCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGgcaa                >  1:521376/6‑150 (MQ=38)                                                                                                                                  gCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTtgtg                                                                                                                   >  2:1139958/1‑51 (MQ=255)                                                                                                                                  gCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTtgtg                                                                                                                   <  1:1139958/51‑1 (MQ=255)                                                                                                                                  gCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTTGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGGCGGCCCGATGAAAAGGCCCATGAAGCCAAAGgcaa                >  2:566893/1‑150 (MQ=255)                                                                                                                                  gCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGaa                           <  2:390813/139‑1 (MQ=255)                                                                                                                                  gCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGaa                           >  1:390813/1‑139 (MQ=255)                                                                                                                                   ccctttCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGaaaa                                     <  1:1281556/124‑1 (MQ=38)                                                                                                                                   ccctttCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGaaaa                                     >  2:1281556/5‑128 (MQ=38)                                                                                                                                   ccctttCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGgcaag               >  2:769480/5‑150 (MQ=38)                                                                                                                                   ccctttCGCGGGCAAGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGgcaag               >  1:124149/5‑150 (MQ=255)                                                                                                                                   ccTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGgcaag               >  2:591651/1‑150 (MQ=255)                                                                                                                                    ctctTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCtccagtcca                                                                                  <  1:1207376/82‑1 (MQ=255)                                                                                                                                    ctctTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCtccagtcca                                                                                  >  2:1207376/1‑82 (MQ=255)                                                                                                                                    cgggtCGCGGGCAAGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTa                                                                <  1:797844/96‑1 (MQ=39)                                                                                                                                    cctttCGCGGGCAAGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTa                                                                >  2:797844/4‑100 (MQ=38)                                                                                                                                     tctTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCCGCTGCTACGCCGCGTTACGGCTCCAGTCAAGCAGCAGGCGGTAGCCTACCGCCAGAAAGGTCGGGCCGCTGAAAAGGCCAATGAAGCCAAAGgcaagca             >  2:431954/1‑150 (MQ=255)                                                                                                                                           ggggCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAgccgc       <  1:626577/149‑1 (MQ=255)                                                                                                                                                ttGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAac  >  2:368567/1‑150 (MQ=255)                                                                                                                                                |                                                                                                                                                      TCTCCCCGTGTTACGGCTTGGGCCGCTTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAAC  >  NC_002947/4705316‑4705608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑