Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,705,470 A→G 19.6% intergenic (‑162/+15) kdpF ← / ← PP_4162 potassium‑transporting ATPase subunit F/membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,705,4700AG19.6% 90.0 / 16.9 46intergenic (‑162/+15)kdpF/PP_4162potassium‑transporting ATPase subunit F/membrane protein
Reads supporting (aligned to +/- strand):  ref base A (19/18);  new base G (5/4);  total (24/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.08e-01

CCGTGTTACGGCTTGGGCCGCTTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAACACGCCCAGCA  >  NC_002947/4705321‑4705618
                                                                                                                                                     |                                                                                                                                                    
ccGTGTTCCGGCTGGGGCCGCTTTGGGGAAATTGTCCGAGGGAGGGGCTAAAAGGGGCGAGATGGGGGGGTGGGGCTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGaaa                                                                                                                                                      <  1:99281/150‑1 (MQ=255)
     ttAGGGCGTGGGCGGTGTTGGGGAAATTGCCCGAAGGAGGGGTGTAAGGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCTTAAAAATTAGCTTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGcc                                                                                                                                                 <  2:275727/150‑1 (MQ=255)
       cggccTTGGGCCGTTTGGGGGAAATTGTCGGAGGAAGAGGCGAAAAGGGGCGAGAGGGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGt                                                                                                                                               <  1:442719/146‑1 (MQ=255)
              gggCGGCTGGGGGGACGTTGGCAGGGGGGGCGGGGTAGAGGGGGGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGc                                                                                                                                        <  1:1118083/150‑1 (MQ=255)
               ggcggcTTGGGGGAAATTGGCCGGGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCCTTTCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGca                                                                                                                                       <  2:600947/150‑2 (MQ=25)
               ggCCGGTTGGGGGAAATTGTCGGGGGGGGAGGGGAAAAGGGGCGAGAGGGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCt                                                                                                                                       <  2:1336674/150‑1 (MQ=255)
                gCCGCTTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGGTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCtt                                                                                                                                      >  1:989490/1‑150 (MQ=255)
                        ggagAAACTGTGCGCAGGAGTGGGGTATAGGGGTGTGTTCGGGGTGGGGAGGTCGGTATAAAGAATGCGTAAACATCCGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATtgttgt                                                                                                                              <  1:103304/147‑1 (MQ=255)
                           gAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTAttgagatcgg                                                                                                                           >  1:1045948/1‑143 (MQ=255)
                                  tCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGgctgct                                                                                                                    >  1:188440/1‑150 (MQ=255)
                                  tCCGAGGGAGAGGAGTAAAGGGGCGACGGCGGGGGGTGGGGGTGGGAATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGgctgct                                                                                                                    <  1:551396/150‑1 (MQ=255)
                                     gAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCCTTTCGCGGGCAAGCCCGCGAAGAGGCCGTTACAg                                                                                                                                          <  2:963133/125‑1 (MQ=25)
                                     gAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCCTTTCGCGGGCAAGCCCGCGAAGAGGCCGTTACAg                                                                                                                                          >  1:963133/1‑125 (MQ=25)
                                       gggAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGATGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCCTATTGTTGTGGCGGCTGCTGcgcc                                                                                                               >  2:675948/1‑150 (MQ=255)
                                                                ggggggTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCcagcagca                                                                                      <  2:188440/150‑1 (MQ=255)
                                                                ggggggTGGGGGGGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCcagcagca                                                                                      <  2:737451/150‑1 (MQ=255)
                                                                  ggggggggggggTGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAgg                                                                                    <  1:1064434/150‑1 (MQ=255)
                                                                         gggtgggtATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGc                                                                             <  2:1063553/150‑1 (MQ=255)
                                                                                  taAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAgg                                                                                    >  1:590573/1‑134 (MQ=255)
                                                                                  taAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGCCGGCTGCTGCTCCGCGTTACGGCTCCAGTCCAGCAGCAgg                                                                                    <  2:590573/134‑1 (MQ=255)
                                                                                        aTGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGgct                                                                                                                       <  2:870097/93‑1 (MQ=255)
                                                                                        aTGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGgct                                                                                                                       >  1:870097/1‑93 (MQ=255)
                                                                                                                cTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGGTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGGCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGa                                      >  2:762257/1‑150 (MQ=255)
                                                                                                                    cTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGcc                                  >  1:1217710/1‑150 (MQ=255)
                                                                                                                        aaCGGCCCTTTCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTCACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAg                              <  2:813802/150‑1 (MQ=38)
                                                                                                                           ggCTTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATAGTTGTGGCGGCTGCCGCACCGGATTACGGCTCCAATCCAGCAGCACGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATCAAGCCAAAGgcc                           >  2:520187/1‑149 (MQ=255)
                                                                                                                            gccctttCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGgcaa                          >  2:443355/6‑150 (MQ=38)
                                                                                                                            gccctttCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGgcaa                          >  1:521376/6‑150 (MQ=38)
                                                                                                                            gCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTtgtg                                                                                                                             <  1:1139958/51‑1 (MQ=255)
                                                                                                                            gCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTtgtg                                                                                                                             >  2:1139958/1‑51 (MQ=255)
                                                                                                                            gCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTTGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGGCGGCCCGATGAAAAGGCCCATGAAGCCAAAGgcaa                          >  2:566893/1‑150 (MQ=255)
                                                                                                                            gCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGaa                                     >  1:390813/1‑139 (MQ=255)
                                                                                                                            gCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGaa                                     <  2:390813/139‑1 (MQ=255)
                                                                                                                             ccctttCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGaaaa                                               >  2:1281556/5‑128 (MQ=38)
                                                                                                                             ccctttCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGaaaa                                               <  1:1281556/124‑1 (MQ=38)
                                                                                                                             ccctttCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGgcaag                         >  2:769480/5‑150 (MQ=38)
                                                                                                                             ccctttCGCGGGCAAGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGgcaag                         >  1:124149/5‑150 (MQ=255)
                                                                                                                             ccTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGgcaag                         >  2:591651/1‑150 (MQ=255)
                                                                                                                              ctctTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCtccagtcca                                                                                            <  1:1207376/82‑1 (MQ=255)
                                                                                                                              ctctTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCtccagtcca                                                                                            >  2:1207376/1‑82 (MQ=255)
                                                                                                                              cgggtCGCGGGCAAGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTa                                                                          <  1:797844/96‑1 (MQ=39)
                                                                                                                              cctttCGCGGGCAAGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTa                                                                          >  2:797844/4‑100 (MQ=38)
                                                                                                                               tctTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCCGCTGCTACGCCGCGTTACGGCTCCAGTCAAGCAGCAGGCGGTAGCCTACCGCCAGAAAGGTCGGGCCGCTGAAAAGGCCAATGAAGCCAAAGgcaagca                       >  2:431954/1‑150 (MQ=255)
                                                                                                                                     ggggCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAgccgc                 <  1:626577/149‑1 (MQ=255)
                                                                                                                                          ttGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAac            >  2:368567/1‑150 (MQ=255)
                                                                                                                                          aagCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAac            <  2:124149/148‑1 (MQ=255)
                                                                                                                                              ccGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGAAAGCCGCCGAACACGc        >  1:1423130/1‑150 (MQ=255)
                                                                                                                                                    aaGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTCACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAACACGCCcagca  <  1:675948/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCGTGTTACGGCTTGGGCCGCTTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAACACGCCCAGCA  >  NC_002947/4705321‑4705618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑