Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,731,726 T→C 6.1% N361D (AAT→GAT)  sucB ← 2‑oxoglutarate dehydrogenase dihydrolipoyltranssuccinylase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,731,7260TC 6.1% 191.3 / 2.2 65N361D (AAT→GAT) sucB2‑oxoglutarate dehydrogenase dihydrolipoyltranssuccinylase subunit
Reads supporting (aligned to +/- strand):  ref base T (30/31);  new base C (2/2);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

TTAGATGTCCAGCAGCAGGCGGGACGGATCTTCCAGCAGGTTCTTGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGACGATCGGGGTCGACATCATCGAACCGAAGGTGCCACCGTTGGTGATGGTGAAGGTGCCACCGGTCATCTCTTCGATGGACAGTTTGC  >  NC_002947/4731583‑4731875
                                                                                                                                               |                                                                                                                                                     
ttAGATGTCCAGCAGCAGGCGGGACGGATCTTCCAGCAGGTTCTTGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACATCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGc                                                                                                                                                 >  1:180753/1‑150 (MQ=255)
   gATGTCCAGCAGCAGGCGGGACGGATCTTCCAGCAGGTTCTTGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATCAATGGCCag                                                                                                                                              <  2:815083/150‑2 (MQ=255)
    aTGTCCAGCAGCAGGCGGGACGGATCTTCCAGCAGGTTCTTGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATCAATGGCCagg                                                                                                                                             <  1:1158849/150‑3 (MQ=255)
      gTCCAGCAGCAGGCGGGACGGATCTTCCAGCAGGTTCTTGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCgg                                                                                                                                           <  2:1395105/150‑1 (MQ=255)
                 ggCGGGACGGATCTTCCAGCAGGTTCTTGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACAACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGgatga                                                                                                                                <  2:1110189/150‑1 (MQ=255)
                     ggACGGATCTTCCAGCAGGTTCTTGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGAtgtt                                                                                                                            >  2:1036091/1‑150 (MQ=255)
                       aCGGATCTTCCAGCAGGTTCTTGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGAtgttgt                                                                                                                          <  2:617059/150‑1 (MQ=255)
                              ttCCAGCAGGTTCTTGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGcc                                                                                                                   >  2:1216137/1‑150 (MQ=255)
                                  agcagGTTCTTGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATc                                                                                                                                             >  1:1137685/1‑120 (MQ=255)
                                  agcagGTTCTTGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATc                                                                                                                                             <  2:1137685/120‑1 (MQ=255)
                                        ttCTTGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGAtgttg                                                                                                                           <  2:164199/132‑1 (MQ=255)
                                        ttCTTGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGAtgttg                                                                                                                           >  1:164199/1‑132 (MQ=255)
                                        ttCTTGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATggcg                                                                                                         >  2:634923/1‑150 (MQ=255)
                                         tCTTGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATggcgg                                                                                                        >  2:364128/1‑150 (MQ=255)
                                            tGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCt                                                                                                     >  1:1110717/1‑150 (MQ=255)
                                             gATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGAtgttg                                                                                                                           >  2:656968/1‑127 (MQ=255)
                                             gATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGAtgttg                                                                                                                           <  1:656968/127‑1 (MQ=255)
                                               tAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTgcg                                                                                                  >  2:164348/1‑150 (MQ=255)
                                                   gACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGcggcgg                                                                                              <  1:1036091/150‑1 (MQ=255)
                                                   gACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGcggcgg                                                                                              <  1:700059/150‑1 (MQ=255)
                                                       aGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGACGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTg                                                                                          <  2:1244070/150‑1 (MQ=255)
                                                        ggAAGGTTCCCGCGTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGa                                                                                         <  1:425960/150‑1 (MQ=255)
                                                        ggAAGGTTACCGCTTCCTTGCCGTCGATCGGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGa                                                                                         >  2:42668/1‑150 (MQ=255)
                                                        ggAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGa                                                                                         <  1:164348/150‑1 (MQ=255)
                                                        ggAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGa                                                                                         <  1:1373923/150‑1 (MQ=255)
                                                              ttACCGCTTCCTTGCCGCCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGa                                                                                         <  2:130878/144‑1 (MQ=255)
                                                              ttACCGCTTCCTTGCCGCCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGa                                                                                         >  1:130878/1‑144 (MQ=255)
                                                                aCCGCTTCCTTGCCGTCGATCGGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGACGATCggg                                                                                 <  1:42668/150‑1 (MQ=255)
                                                                  cGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGACGATCGGGGt                                                                               >  2:94668/1‑150 (MQ=255)
                                                                  cGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGACGATCGGGGt                                                                               >  2:17547/1‑150 (MQ=255)
                                                                  cGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGACGATCGGGGt                                                                               <  1:94668/150‑1 (MQ=255)
                                                                    cTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCg                                                                                                                                            <  1:1199106/87‑1 (MQ=255)
                                                                    cTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCg                                                                                                                                            >  2:1199106/1‑87 (MQ=255)
                                                                        cTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGtt                                                                                           >  2:189178/1‑132 (MQ=255)
                                                                        cTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGtt                                                                                           <  1:189178/132‑1 (MQ=255)
                                                                            cttccGATCTGGCGGTGATCGTACGACAGCGCCAGGTACATCATAGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGACGATCGGGGTCGACatcatc                                                                     <  1:1224174/146‑1 (MQ=255)
                                                                                       gCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATggcg                                                                                                         >  2:359734/1‑103 (MQ=255)
                                                                                       gCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATggcg                                                                                                         <  1:359734/103‑1 (MQ=255)
                                                                                       gCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGACGATCGGGGTCGACATCATCGAACCGAAGGt                                                          >  1:1370539/1‑150 (MQ=255)
                                                                                       gCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGACGATCGGGGTCGACATCATCGAACCGAAGGt                                                          >  1:510095/1‑150 (MQ=255)
                                                                                         ggTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGACGATCGGGGTCGACATCATCGAACCGAAGGTGc                                                        <  1:71654/150‑1 (MQ=255)
                                                                                                 tACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGatgatg                                                                                                                              <  1:1128848/72‑1 (MQ=255)
                                                                                                 tACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGatgatg                                                                                                                              >  2:1128848/1‑72 (MQ=255)
                                                                                                  aCGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGACGATCGGGGTCGACATCATCGAACCGAAGGTGCCACCGTtgg                                               <  1:1216137/150‑1 (MQ=255)
                                                                                                     aCAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCga                                                                                                                 >  2:1163854/1‑81 (MQ=255)
                                                                                                     aCAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCga                                                                                                                 <  1:1163854/81‑1 (MQ=255)
                                                                                                      cAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGACGCTCGGGGTCGACatcatc                                                                     <  2:729749/124‑1 (MQ=255)
                                                                                                      cAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGACGATCGGGGTCGACatcatc                                                                     >  1:729749/1‑124 (MQ=255)
                                                                                                       aGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGACGATCGGGGTCGACATCATCGAACCGAAGGTGCCACCGTtggtgatg                                          <  1:563287/150‑1 (MQ=255)
                                                                                                        gcgcCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGACGATCGGGGTCGACATCATCGAACCGAAGGTGCCACCGTtggtgatgg                                         >  1:457744/1‑150 (MQ=255)
                                                                                                            cAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGACGATCGGGGTCGACATCATCGAACCGAAGGTGCCACCGTTGGTGATGGTGaa                                     >  2:232824/1‑150 (MQ=255)
                                                                                                               gTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGACGATCTGGGTCGACATCATCGAACCGAAGGTGCCACCGTTGGTGATGGTGAAGGt                                  <  2:112504/150‑1 (MQ=255)
                                                                                                               gTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGACGATCGGGGTCGACATCATCGAACCGAAGGTGCCACCGTTGGTGATGGTGAAGGt                                  <  1:634923/150‑1 (MQ=255)
                                                                                                                 aCATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGACGATCGGGGTCGACATCATCGAACCGAAGGTGCCACCGTTGGTGATGGTGAAGGTGc                                <  1:232824/150‑1 (MQ=255)
                                                                                                                         gggCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGACGATCGGGGTCGACATCATCGAACCGAAGGTGCCACCGTTGGTGATGGTGAAGGTGCCACCGGTc                        >  1:15190/1‑150 (MQ=255)
                                                                                                                             gAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGACGATCGGGGTCGACATCATCGAACCGAAGGTGCCACCGTtg                                                <  2:1213091/122‑1 (MQ=255)
                                                                                                                             gAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGACGATCGGGGTCGACATCATCGAACCGAAGGTGCCACCGTtg                                                >  1:1213091/1‑122 (MQ=255)
                                                                                                                                    ccgatGGCCATCAATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGAAACCTGCGGCGGGGTAAAGAGCGTGGGCGACATCATCGAACCGAAGGTGCCACCGTTGGTGATGGTGAAGGTGCCACCGGTCATCTCTTCGAt             >  1:531341/5‑150 (MQ=255)
                                                                                                                                      gatGGCCATCAATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGACGATCGGGGTCGACATCATCCAACCGCAGGTGCCACCGTTGGTGATAGTGGAGGTGCCACCGGTCATCTCTTCGATgg           >  2:472609/3‑150 (MQ=255)
                                                                                                                                       cTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGACGATCGGGGTCGACATCATCGAACCGAAGGTGCCACCGTTGGTGATGGTGAAGGTGCCACCGGTCATCTCTTCGATGGa          >  1:153998/1‑150 (MQ=255)
                                                                                                                                            cATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGACGATCGGGGTCGACATCATCGAACCGCGAGTGCCACCGTTGGTGATGGGGAAGGTGCCACCGGTCATCTCTTCGATGGACAGtt     >  1:728466/1‑150 (MQ=255)
                                                                                                                                             aTTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGACGATCGGGGTCGACATCATCGAACCGAAGGt                                                          <  2:1055065/96‑1 (MQ=255)
                                                                                                                                             aTTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGACGATCGGGGTCGACATCATCGAACCGAAGGt                                                          >  1:1055065/1‑96 (MQ=255)
                                                                                                                                             aTTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGACGATCGGGGTCGACATCATCGAACCGAAGGTGCCACCGTTGGTGATGGTGaa                                     <  2:1187392/117‑1 (MQ=255)
                                                                                                                                             aTTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGACGATCGGGGTCGACATCATCGAACCGAAGGTGCCACCGTTGGTGATGGTGaa                                     >  1:1187392/1‑117 (MQ=255)
                                                                                                                                               tGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGACGATCGGGGTCGACATCATCGAACCGAAGGTGCCACCGTTGGTGATGGTGAAGGTGCCACCGGTCATCTCTTCGATGGACAGTTTGc  <  2:634480/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
TTAGATGTCCAGCAGCAGGCGGGACGGATCTTCCAGCAGGTTCTTGATAGTGACCAGGAAGGTTACCGCTTCCTTGCCGTCGATCAGGCGGTGATCGTACGACAGCGCCAGGTACATCATCGGGCGAATCACGACCTGGCCATTGATGGCCATCGGGCGCTGGATGATGTTGTGCATGCCGAGAATGGCGGCCTGCGGCGGGTTGACGATCGGGGTCGACATCATCGAACCGAAGGTGCCACCGTTGGTGATGGTGAAGGTGCCACCGGTCATCTCTTCGATGGACAGTTTGC  >  NC_002947/4731583‑4731875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑