Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,744,652 G→T 10.0% G73G (GGC→GGA)  PP_4198 ← OmpA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,744,6520GT10.0% 173.7 / 2.7 56G73G (GGC→GGA) PP_4198OmpA family protein
Reads supporting (aligned to +/- strand):  ref base G (28/22);  new base T (2/4);  total (30/26)
Fisher's exact test for biased strand distribution p-value = 4.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCCTCACGCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGAC  >  NC_002947/4744505‑4744800
                                                                                                                                                   |                                                                                                                                                    
ggCCTCACGCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAACTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGCACCGGTTcc                                                                                                                                                    <  2:1027118/150‑1 (MQ=255)
  ccTCACGCAAGGCGAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCATGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCTCTGGCTGAGATAAGCATAGTGTTGTACAGCGGAACAGGTGCCGa                                                                                                                                                  >  2:234087/1‑150 (MQ=255)
  ccTCACGCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGCACCGGTTCCGa                                                                                                                                                  >  1:732568/1‑150 (MQ=255)
     cACGCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGCACCGGTTCCGATGt                                                                                                                                               <  2:732568/150‑1 (MQ=255)
     cACGCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGCACCGGTTCCGATGt                                                                                                                                               <  2:1363095/150‑1 (MQ=255)
      aCGCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGCACCGGTTCCGATGtg                                                                                                                                              <  1:1420877/150‑2 (MQ=255)
       cGCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCATGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTaa                                                                                                                                             <  1:865547/150‑1 (MQ=255)
        gCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAAc                                                                                                                                            >  1:359331/1‑150 (MQ=255)
              ccAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACa                                                                                                                                      >  1:1264926/1‑150 (MQ=255)
              ccAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACa                                                                                                                                      <  2:1103453/150‑1 (MQ=255)
                 gCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGgc                                                                                                                                   <  2:359331/150‑1 (MQ=255)
                       ggcgctggcgctCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTGAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCgg                                                                                                                             <  2:958336/150‑1 (MQ=255)
                                gctCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCa                                                                                                                    >  1:283752/1‑150 (MQ=255)
                                 ctCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAg                                                                                                                   >  1:380367/1‑150 (MQ=255)
                                    cAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGa                                                                                                                >  1:191814/1‑150 (MQ=255)
                                            gCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCAcccg                                                                                                        >  1:556384/1‑150 (MQ=255)
                                                ggCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGgcgc                                                                                                                                 <  1:645037/121‑1 (MQ=255)
                                                ggCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCCGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGgcgc                                                                                                                                 >  2:645037/1‑121 (MQ=255)
                                                         cTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAAtcacgtc                                                                                           >  1:855309/1‑150 (MQ=255)
                                                                ttCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACAGGTTCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACgcggc                                                                                    >  1:601711/1‑150 (MQ=255)
                                                                      gcCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAAGGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTg                                                                              <  2:328812/150‑1 (MQ=255)
                                                                          gCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCgca                                                                          <  1:296402/150‑1 (MQ=255)
                                                                          gCTTGGCATGCTCGCGGGCAATCCCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAAt                                                                                                 >  1:378995/1‑127 (MQ=255)
                                                                          gCTTGGCATGCTCGCGGGCAATCCCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAAt                                                                                                 <  2:378995/127‑1 (MQ=255)
                                                                           cTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCgcag                                                                         <  2:380367/150‑1 (MQ=255)
                                                                               gCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGcaca                                                                     >  1:439970/1‑150 (MQ=255)
                                                                                  tGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCg                                                                  <  2:191814/150‑1 (MQ=255)
                                                                                  tGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCg                                                                  >  2:631292/1‑150 (MQ=255)
                                                                                     tCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCTGCGCGAGCCAGCGACTCACCCGCACGAATCACGTCACGCGGCGCGCTGCGCAGCCCCTCgcat                                                               >  2:63160/1‑147 (MQ=255)
                                                                                           gCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCgcag                                                                         <  2:251936/134‑1 (MQ=255)
                                                                                           gCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCgcag                                                                         >  1:251936/1‑134 (MQ=255)
                                                                                            cAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCtt                                                        <  1:796330/150‑1 (MQ=255)
                                                                                              agctcGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTg                                                      >  1:1037932/3‑150 (MQ=255)
                                                                                                     tGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCAcccgcc                                                                                                      >  1:1385433/1‑95 (MQ=255)
                                                                                                     tGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCAcccgcc                                                                                                      <  2:1385433/95‑1 (MQ=255)
                                                                                                         gcgcTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGaa                                           >  2:577388/1‑150 (MQ=255)
                                                                                                          cgcTGGCGGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAg                                          <  2:439970/150‑1 (MQ=255)
                                                                                                                 tgAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACgcgg                                                                                     >  2:1004320/1‑100 (MQ=255)
                                                                                                                 tgAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACgcgg                                                                                     <  1:1004320/100‑1 (MQ=255)
                                                                                                                 tgAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGCAGGCAACgg                                   >  1:66647/1‑150 (MQ=255)
                                                                                                                 tgAGGTAAGCATAATGCCGCACATCGGAACCGGGGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACgg                                   <  1:719407/150‑1 (MQ=255)
                                                                                                                     gTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGc                               >  1:1283608/1‑150 (MQ=255)
                                                                                                                     gTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGc                               >  1:994100/1‑150 (MQ=255)
                                                                                                                      tAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGcc                              <  1:577388/150‑1 (MQ=255)
                                                                                                                          cATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCAt                          >  2:247185/1‑150 (MQ=255)
                                                                                                                          cATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCAt                          >  2:757360/1‑150 (MQ=255)
                                                                                                                               tGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTgc                                                                             >  2:395253/1‑94 (MQ=255)
                                                                                                                               tGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTgc                                                                             <  1:395253/94‑1 (MQ=255)
                                                                                                                                   gCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGt                                  >  2:261963/1‑133 (MQ=255)
                                                                                                                                   gCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGt                                  <  1:261963/133‑1 (MQ=255)
                                                                                                                                       aTCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCAct             >  1:682742/1‑150 (MQ=255)
                                                                                                                                        tCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCActg            >  1:436839/1‑150 (MQ=255)
                                                                                                                                        tCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCActg            >  2:1031456/1‑150 (MQ=255)
                                                                                                                                             aCCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTgcg       <  2:855309/150‑1 (MQ=255)
                                                                                                                                                 gTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGa   <  1:863011/150‑1 (MQ=255)
                                                                                                                                                  tGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGAc  <  1:631292/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GGCCTCACGCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGAC  >  NC_002947/4744505‑4744800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑