Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,779,856 A→G 8.7% N2501N (AAT→AAC)  pvdJ ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,779,8560AG 8.7% 123.9 / 2.2 46N2501N (AAT→AAC) pvdJnon‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base A (19/23);  new base G (2/2);  total (21/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01

TCATCGCCCAGACTGATCCGCTCGACGCCCAGCACCTCGCTCCAGACCTGCGCCAATTGCTGCTGCAACGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCATCGCGGGCGACCCAGTAGGCCACCAACT  >  NC_002947/4779710‑4780004
                                                                                                                                                  |                                                                                                                                                    
tCATCGCCCAGACTGATCCGCTCGACGCCCAGCACCTCGCTCCAGACCTGCGCCAATTGCTGCTGCAACGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTg                                                                                                                                                   <  2:1028763/150‑1 (MQ=255)
  aTCGCCCAGACTGATCCGCTCGACGCCCAGCACCTCGCTCCAGACCTGCGCCAATTGCTGCTGCAACGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGCTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGc                                                                                                                                                 >  1:1014179/1‑150 (MQ=255)
   tCGCCCAGACTGATCCGCTCGACGCCCAGCACCTCGCTCCAGACCTGCGCCAATTGCTGCTGCAACGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCa                                                                                                                                                <  1:280921/150‑1 (MQ=255)
      cccAGACTGATCCGCTCGACGCCCAGCACCTCGCTCCAGACCTGCGCCAATTGCTGCTGCAACGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTc                                                                                                                                             <  2:637865/150‑1 (MQ=255)
          gACTGATCCGCTCGACGCCCAGCACCTCGCTCCAGACCTGCGCCAATTGCTGCTGCAACGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCg                                                                                                                                         >  1:99427/1‑150 (MQ=255)
             tGATCCGCTCGACGCCCAGCACCTCGCTCCAGACCTGCGCCAATTGCTGCTGCAACGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCa                                                                                                                                      <  1:341182/150‑1 (MQ=255)
                tCCGCTCGACGCCCAGCACCTCGCTCCAGACCTGCGCCAATTGCTGCTGCAACGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGc                                                                                                                                   >  1:258326/1‑150 (MQ=255)
                   gCTCGACGCCCAGCACCTCGCTCCAGACCTGCGCCAATTGCTGCTGCAACGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCAt                                                                                                                                                     >  1:647307/1‑129 (MQ=255)
                   gCTCGACGCCCAGCACCTCGCTCCAGACCTGCGCCAATTGCTGCTGCAACGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCAt                                                                                                                                                     <  2:647307/129‑1 (MQ=255)
                   gCTCGACGCCCAGCACCTCGCTCCAGACCTGCGCCAATTGCTGCTGCAACGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGGGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGt                                                                                                                                >  2:798772/1‑150 (MQ=255)
                      cGACGCCCAGCACCTCGCTCCAGACCTGCGCCAATTGCTGCTGCAACGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGa                                                                                                                             <  2:172932/150‑1 (MQ=255)
                      cGACGCCCAGCACCTCGCTCCAGACCTGCGCCAATTGCTGCTGCAACGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGa                                                                                                                             <  2:840603/150‑1 (MQ=255)
                        aCGCCCAGCACCTCGCTCCAGACCTGCGCCAATTGCTGCTGCAACGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGc                                                                                                                           <  2:412458/150‑1 (MQ=255)
                             cAGCACCTCGCTCCAGACCTGCGCCAATTGCTGCTGCAACGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCAc                                                                                                                      >  1:946455/1‑150 (MQ=255)
                              aGCACCTCGCTCCAGACCTGCGCCAATTGCTGCTGCAACGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCAcc                                                                                                                     <  1:1356908/150‑1 (MQ=255)
                                  ccTCGCTCCAGACCTGCGCCAATTGCTGCTGCAACGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCcaca                                                                                                                 <  2:457692/150‑1 (MQ=255)
                                    tCGCTCCAGACCTGCGCCAATTGCTGCTGCAACGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGt                                                                                                               <  2:258326/150‑1 (MQ=255)
                                        tCCAGACCTGCGCCAATTGCTGCTGCAACGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGTTCACAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCg                                                                                                           >  1:1314758/1‑150 (MQ=255)
                                               cTGCGCCAATTGCTGCTGCAACGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCAt                                                                                                    >  2:138285/1‑150 (MQ=255)
                                                      aaTTGCTGCTGCAACGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGccc                                                                                             <  1:263022/150‑1 (MQ=255)
                                                       aTTGCTGCTGCAACGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCg                                                                                            <  1:138285/150‑1 (MQ=255)
                                                            tgctgcAACGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCa                                                                                                                                                <  2:1373475/93‑1 (MQ=255)
                                                            tgctgcAACGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCa                                                                                                                                                >  1:1373475/1‑93 (MQ=255)
                                                               tgcAACGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTg                                                                                    >  1:329285/1‑150 (MQ=255)
                                                                   acgacgTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCAc                                                                                >  2:257943/1‑150 (MQ=255)
                                                                     gacgTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGc                                                                              >  2:941973/1‑150 (MQ=255)
                                                                             cGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTc                                                                      <  2:329285/150‑1 (MQ=255)
                                                                              ggTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCg                                                                     <  2:99427/150‑1 (MQ=255)
                                                                                      gTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCAc                                                             >  1:1276194/1‑150 (MQ=255)
                                                                                       tAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACg                                                            >  2:358363/1‑150 (MQ=255)
                                                                                            ctgctgAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTgcg                                                       >  1:200147/1‑150 (MQ=255)
                                                                                               ctgAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCAt                                                    <  1:358363/150‑1 (MQ=255)
                                                                                                         cTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTgcg                                          <  2:946455/150‑1 (MQ=255)
                                                                                                                 cGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGa                                  <  2:1276194/150‑1 (MQ=255)
                                                                                                                   gTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAgg                                >  1:46958/1‑150 (MQ=255)
                                                                                                                       cGGCAATGCTTTGCGGTCGAGCTTGCCGTTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCAt                             >  1:735531/1‑149 (MQ=255)
                                                                                                                       cGGCAATGCTTTGCGGTCGAGCTTGCCGTTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCAt                             >  1:734524/1‑149 (MQ=255)
                                                                                                                       cGGCAATGCTTTGCGGTCGAGCTTGCCGTTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCAt                             <  2:734524/149‑1 (MQ=255)
                                                                                                                       cGGCAATGCTTTGCGGTCGAGCTTGCCGTTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCAt                             <  2:735531/149‑1 (MQ=255)
                                                                                                                           aaTGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCATCGCgg                        <  1:257943/150‑1 (MQ=255)
                                                                                                                             tGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCATCGCGGGc                      <  2:200147/150‑1 (MQ=255)
                                                                                                                                       tCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCATCGCGGGCGACCCAGTAg            <  1:55892/150‑1 (MQ=255)
                                                                                                                                           gCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCATCGCGGGCGACCCAGTAGGcca        >  1:72456/1‑150 (MQ=255)
                                                                                                                                               gCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCATCGCGGGCGACCCAGTAGGCCACCaa    >  2:980971/1‑150 (MQ=255)
                                                                                                                                                 cATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCATCGCGGGCGACCCAGTAGGCCACCAACt  >  2:674061/1‑150 (MQ=255)
                                                                                                                                                 cATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCATCGCGGGCGACCCAGTAGGCCACCAACt  <  2:1314758/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TCATCGCCCAGACTGATCCGCTCGACGCCCAGCACCTCGCTCCAGACCTGCGCCAATTGCTGCTGCAACGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCATCGCGGGCGACCCAGTAGGCCACCAACT  >  NC_002947/4779710‑4780004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑