Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	428,785	G→A	7.2%	S75S (AGC→AGT)	PP_0351 ←	transmembrane sensor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	428,785	0	G	A	7.2%	197.5 / 2.1	68	S75S (AGC→AGT)	PP_0351	transmembrane sensor
Reads supporting (aligned to +/- strand):  ref base G (26/37);  new base A (3/2);  total (29/39)
Fisher's exact test for biased strand distribution p-value = 6.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

AAGGTGCGTAATTCACCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCG  >  NC_002947/428639‑428930                                                                                                                                                   |                                                                                                                                                    aaGGTGCGTAATTCACCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTg                                                                                                                                                  <  2:278437/150‑1 (MQ=255)   ggTGCGTAATTCACCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGgc                                                                                                                                                <  2:328702/150‑1 (MQ=255)       cGTAATTCACCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGgcgcgc                                                                                                                                            <  1:591587/150‑1 (MQ=255)          aaTTCACCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGAt                                                                                                                                         <  1:31301/150‑1 (MQ=255)           aTTCACCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGAtt                                                                                                                                        <  2:823135/150‑1 (MQ=255)           aTTCACCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGCTGCCAGAGCAGGGGCGCCGCCCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGAtt                                                                                                                                        <  2:519392/150‑1 (MQ=255)              cACCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTg‑ca                                                                                                                                    <  2:88577/150‑1 (MQ=255)               aCCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGACAACCTTGCAAATGCGTCGCCGCAGACAGGCTCGCGATCg‑cag                                                                                                                                   >  2:1374206/1‑150 (MQ=255)                 ccGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTg‑cagca                                                                                                                                 >  2:211932/1‑150 (MQ=255)                 ccGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTg‑cagca                                                                                                                                 <  2:1376578/150‑1 (MQ=255)                 ccGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTg‑cagca                                                                                                                                 <  2:117891/150‑1 (MQ=255)                        ggTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTg                                                                                                                          <  2:459698/150‑1 (MQ=255)                             gcTGGTCGGCCAGCCAGTAGCTGGGTACCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑Tg                                                                                                                    <  1:1037172/150‑1 (MQ=255)                                 gTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCGGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCgg                                                                                                                <  1:677649/150‑1 (MQ=255)                                      ccagccagTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAg                                                                                                           >  1:1068720/1‑150 (MQ=255)                                       cagccagTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGc                                                                                                          >  2:204249/1‑150 (MQ=255)                                       cagccagTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGc                                                                                                          >  1:538049/1‑150 (MQ=255)                                             gTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCcgc                                                                                                    <  2:992781/150‑1 (MQ=255)                                                 cTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTa                                                                                                >  1:786452/1‑150 (MQ=255)                                                         cAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTg‑cagc                                                                                                                                  >  2:1320766/1‑109 (MQ=255)                                                         cAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTg‑cagc                                                                                                                                  <  1:1320766/109‑1 (MQ=255)                                                         cAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTg                                                                                        <  2:1160650/150‑1 (MQ=255)                                                         cAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTg                                                                                        <  2:538049/150‑1 (MQ=255)                                                          aGCGACGGGGCGCCGACCCATGCCAGCATCGCGCCGGGCAGAAACGGGCGGGCCAGCCCGCCGCCAACCTTGGGAATGCGTGGCCGCAGACTGGCGCGCGATTG‑TAGACGCTCGTTTCG‑GGCCAAGCCAGCTGCCGCGCTAACGCGTTgg                                                                                       >  2:691295/1‑150 (MQ=255)                                                          aGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTCGCG‑GCGGCGGATACCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTgg                                                                                       >  2:37000/1‑150 (MQ=255)                                                          aGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGACTGGCGCGCGATTG‑CAGCAGCGCGCGTCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTgg                                                                                       >  2:691238/1‑150 (MQ=255)                                                               cGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCAt                                                                                  <  2:1068720/150‑1 (MQ=255)                                                                     gcCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGcc                                                                            <  2:786452/150‑1 (MQ=255)                                                                       cGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGa                                                                          <  1:1374206/150‑1 (MQ=255)                                                                           ccATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGGCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTg                                                                      >  2:126360/1‑150 (MQ=255)                                                                           ccATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTg                                                                      >  2:412997/1‑150 (MQ=255)                                                                           ccATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTg                                                                      >  1:464351/1‑150 (MQ=255)                                                                             aTGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGTCGCCTACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGgc                                                                    >  1:330966/1‑150 (MQ=255)                                                                                ccAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGcc                                                                 >  2:52819/1‑150 (MQ=255)                                                                                 cagcagCGCGCCGAGCAGAAACGTACCATATAGGCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCa                                                                >  1:398239/1‑150 (MQ=255)                                                                                               gCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTg                                                                                                                                       >  2:807838/1‑67 (MQ=255)                                                                                               gCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTg                                                                                                                                       <  1:807838/67‑1 (MQ=255)                                                                                                   aaaCGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTc                                              >  1:294777/1‑150 (MQ=255)                                                                                                   aaaCGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTc                                              <  1:204249/150‑1 (MQ=255)                                                                                                    aaCGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCg                                             <  1:665253/150‑1 (MQ=255)                                                                                                        tGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTg                                                                                                                          <  1:1389523/70‑1 (MQ=255)                                                                                                        tGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTg                                                                                                                          >  2:1389523/1‑70 (MQ=255)                                                                                                          ccGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCGCTgg                                         <  2:1270500/148‑1 (MQ=255)                                                                                                          ccGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCGCTgg                                         >  1:1270500/1‑148 (MQ=255)                                                                                                             gCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCa                                    >  1:2772/1‑150 (MQ=255)                                                                                                              ccAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCAt                                   <  1:437230/150‑1 (MQ=255)                                                                                                              ccAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCAt                                   <  2:464351/150‑1 (MQ=255)                                                                                                                tggccggcgCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGc                                 <  2:922511/147‑1 (MQ=255)                                                                                                                   ccgccgCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAg                              >  1:974417/1‑150 (MQ=255)                                                                                                                    cgccgcCAACCTTGCCAATGCGTCGCCGCTGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAgc                             <  2:330966/150‑1 (MQ=255)                                                                                                                    cgccgcCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGt                                                                                          <  2:870075/89‑1 (MQ=255)                                                                                                                    cgccgcCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGt                                                                                          >  1:870075/1‑89 (MQ=255)                                                                                                                                 gCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGTTCGCCATGCGCTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATACGtt                <  2:760064/150‑1 (MQ=255)                                                                                                                                  ccAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTg               <  1:561054/150‑1 (MQ=255)                                                                                                                                  ccAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTAGCGGGCGGCATGCCAGCGCATGAATTCGTTg               <  1:37000/150‑1 (MQ=255)                                                                                                                                  ccAATGCGTCGCCGCAGACTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGAGTGCGCTGCGCTGGCGGCATGCCAGCGCATGAATTCGTTg               <  1:691238/150‑1 (MQ=255)                                                                                                                                  ccAATGCGTCGCCGCAGACTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGGACGCGGAACTCGCTGGCGGCATGCCAGCGCAGGAATTCGTTg               <  1:691295/150‑1 (MQ=255)                                                                                                                                   cAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGc              >  1:1008889/1‑150 (MQ=255)                                                                                                                                    aaTGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCg             >  1:57482/1‑150 (MQ=255)                                                                                                                                       gCGTCGCCGCAGGCTGGCGCGCGATTG‑CAG‑GGGGCGTGCGGTTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCCTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTtc          <  2:825331/150‑1 (MQ=255)                                                                                                                                          tCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGc              >  2:389140/1‑143 (MQ=255)                                                                                                                                          tCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGc              <  1:389140/143‑1 (MQ=255)                                                                                                                                          tCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTtcatc       >  1:1231367/1‑150 (MQ=255)                                                                                                                                            gccgcAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCGCt                                           >  1:917715/1‑112 (MQ=255)                                                                                                                                            gccgcAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCGCt                                           <  2:917715/112‑1 (MQ=255)                                                                                                                                            gccgcAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAg     <  1:412997/150‑1 (MQ=255)                                                                                                                                             ccgcAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAgg    <  2:294777/150‑1 (MQ=255)                                                                                                                                               gcAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCg  >  2:224084/1‑150 (MQ=255)                                                                                                                                                 gggCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCa                                                            >  1:92826/2‑90 (MQ=255)                                                                                                                                                 gggCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCa                                                            <  2:92826/89‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    AAGGTGCGTAATTCACCCGTGGCGGTGCGCTGGTCGGCCAGCCAGTAGCTGGGTGCCAGCGACGGGGCGCCGACCCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTG‑CAGCAGCGCCTGGCG‑TGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCTGGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCG  >  NC_002947/428639‑428930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑