Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,783,736 C→T 8.6% R1208Q (CGG→CAG)  pvdJ ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,783,7360CT 8.6% 151.5 / 2.9 55R1208Q (CGG→CAG) pvdJnon‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base C (26/24);  new base T (2/3);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGCGCCCATTGGCGCAGGGGTGCCGACCTGGACGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGTGACTACCTGCAGGAGGGCCTGCACCGG  >  NC_002947/4783588‑4783881
                                                                                                                                                    |                                                                                                                                                 
tCGCGCCCATTGGCGCAGGGGTGCCGACCTGGACGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCGGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCg                                                                                                                                                  >  1:455612/1‑150 (MQ=255)
tCGCGCCCATTGGCGCAGGGGTGCCGACCTGGACGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGccc                                                                                                                                                  <  2:802938/150‑2 (MQ=255)
   cgcCCATTGGCGCAGGTGTGCCGACCTGGACGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTACGCAGTTTCCAGGTCTTCAAACGCTATTCGCGAAGACACCTCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTg                                                                                                                                               <  1:174626/150‑1 (MQ=255)
    gcCCATTGGCGCAGGGGTGCCGACCTGGACGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGc                                                                                                                                              >  1:1183411/1‑150 (MQ=255)
     ccaaTTGGCGCAGGGGTGCCGACCTGGACGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCt                                                                                                                                             >  1:1421003/4‑150 (MQ=255)
       cATTGGCGCAGGGGTGCCGACCTGGACGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGa                                                                                                                                           <  1:126347/150‑1 (MQ=255)
         ttGGCGCAGGGGTGCCGACCTGGACGGCATGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACGGCTGTTGCTggcg                                                                                                                                         <  1:332611/150‑4 (MQ=255)
             cgcAGGGGTGCCGACCTGGACGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCg                                                                                                                                     <  2:890762/150‑1 (MQ=255)
               cAGGGGTGCCGACCTGGACGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACACCACTCACCGCCAGGTCATGTACCGCCAGCAACAGCCGTTGCTGACCATCGGc                                                                                                                                   <  1:1316321/150‑1 (MQ=255)
                             tGGACGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGc                                                                                                                                   <  1:1282741/136‑1 (MQ=255)
                             tGGACGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGc                                                                                                                                   >  2:1282741/1‑136 (MQ=255)
                             cctaCGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATg                                                                                                                     >  2:604660/4‑150 (MQ=255)
                              ctaCGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACGGCTGTTGCTggcg                                                                                                                                         >  1:652401/3‑126 (MQ=255)
                              ctaCGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACGGCTGTTGCTggcg                                                                                                                                         <  2:652401/127‑4 (MQ=255)
                                     aGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGtt                                                                                                                              >  1:1410197/1‑133 (MQ=255)
                                     aGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGtt                                                                                                                              <  2:1410197/133‑1 (MQ=255)
                                     aGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTg                                                                                                             >  1:176696/1‑150 (MQ=255)
                                      ggCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGa                                                                                                            >  2:758013/1‑150 (MQ=255)
                                       gCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACAACCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACGATCGGCGAGTTCGAACCATGTCGCGCTGaa                                                                                                           >  2:616877/1‑150 (MQ=255)
                                         tCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGGCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGAAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACa                                                                                                         >  1:1248132/1‑150 (MQ=255)
                                                    ccTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACgg                                                                                              >  1:1034561/1‑150 (MQ=255)
                                                              ccTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGa                                                                                    <  2:176696/150‑1 (MQ=255)
                                                              ccTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGa                                                                                    <  2:1421003/150‑1 (MQ=255)
                                                                tgctgGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGCCCAGCCGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAgg                                                                                  <  1:604660/150‑1 (MQ=255)
                                                                tgctgGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAgg                                                                                  >  2:1278074/1‑150 (MQ=255)
                                                                   tgGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTg                                                                               <  2:1183411/150‑1 (MQ=255)
                                                                      tAGGCCGTCTGCAGGTCTTCGAACAGGATGCCCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGcc                                                                            <  1:1278074/150‑1 (MQ=255)
                                                                                  aGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCCTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTg                                                                <  1:616877/150‑1 (MQ=255)
                                                                                    gTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTggc                                                              >  2:1239531/1‑150 (MQ=255)
                                                                                      cTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTggcgg                                                            >  2:61617/1‑150 (MQ=255)
                                                                                       ttCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTggcggc                                                           >  2:170081/1‑150 (MQ=255)
                                                                                            aCAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCgg                                                                                                                                    <  2:303509/72‑1 (MQ=255)
                                                                                            aCAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCgg                                                                                                                                    >  1:303509/1‑72 (MQ=255)
                                                                                                    cgcCAAGACACCCCATCCACCAACCGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCt                                              <  2:1248132/150‑1 (MQ=255)
                                                                                                        aaGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCaa                                          <  2:1064807/150‑1 (MQ=255)
                                                                                                               cccATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTc                                   >  1:451317/1‑150 (MQ=255)
                                                                                                                   tCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCaga                               <  1:75797/150‑1 (MQ=255)
                                                                                                                   tCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCaga                               <  1:170081/150‑1 (MQ=255)
                                                                                                                       ccaccaGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGTGa                           <  1:1165914/150‑1 (MQ=255)
                                                                                                                       ccaccaGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGTGa                           <  1:61617/150‑1 (MQ=255)
                                                                                                                        caccaGGTGATGCCCCGCCAGCAACAGCTGCTCCAGAACATGGGCGAGTTCGAACAATGCCGCCCTGAGCAGCTCGCCACGGGCAAGGTCGAGGCTCGCCTGCAGCTCGTTGGCGGCGGTTTCTAGCGTTGCAAGCCTGTCCAGAGtccc                          <  2:473535/150‑4 (MQ=255)
                                                                                                                        caccaGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGcc                                                                                           >  2:840313/1‑85 (MQ=255)
                                                                                                                        caccaGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGcc                                                                                           <  1:840313/85‑1 (MQ=255)
                                                                                                                        caccaGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCagag                              <  2:663047/146‑1 (MQ=255)
                                                                                                                        caccaGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCagag                              >  1:663047/1‑146 (MQ=255)
                                                                                                                            aGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCTTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGTGACTAcc                      >  1:942732/1‑150 (MQ=255)
                                                                                                                                  tGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGTGACTACCTGCagg                >  1:979535/1‑150 (MQ=255)
                                                                                                                                   gCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTa                                                   >  1:794014/1‑114 (MQ=255)
                                                                                                                                   gCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTa                                                   <  2:794014/114‑1 (MQ=255)
                                                                                                                                      ccGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGTGACTACCTGCAGGAggg            >  1:50194/1‑150 (MQ=255)
                                                                                                                                         tcAGCAACGGCTGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGGACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGTGACTACCTGCAGGAGGGCCt         >  1:893267/2‑150 (MQ=255)
                                                                                                                                          cAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTgg                                                                              >  2:543524/1‑80 (MQ=255)
                                                                                                                                          cAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTgg                                                                              <  1:543524/80‑1 (MQ=255)
                                                                                                                                          cAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGTGACTACCTGCAGGAGGGCCTg        >  1:569455/1‑150 (MQ=255)
                                                                                                                                               aCAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCCGCAAGGCTGTCCAGAGTGACTACCTGCAGGAGGGCCTGCACCg   <  2:1034561/150‑1 (MQ=255)
                                                                                                                                                cAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGTGACTACCTGCAGGAGGGCCTGCACCgg  >  1:490682/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TCGCGCCCATTGGCGCAGGGGTGCCGACCTGGACGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGTGACTACCTGCAGGAGGGCCTGCACCGG  >  NC_002947/4783588‑4783881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑