| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,793,912 | C→T | 6.9% | intergenic (‑28/+128) | pvdI ← / ← PP_4222 | non‑ribosomal peptide synthetase/protein SyrP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,793,912 | 0 | C | T | 6.9% | 168.8 / 2.8 | 58 | intergenic (‑28/+128) | pvdI/PP_4222 | non‑ribosomal peptide synthetase/protein SyrP |
| Reads supporting (aligned to +/- strand): ref base C (30/24); new base T (2/2); total (32/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.92e-01 | |||||||||||
GATCGGCAACAGCGCGAAATCGACGCCCTCCCCGCGCATGGCATCCAGAAACAACCGTCGCTTGGCAGCGGGCAGCTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCCGCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCG > NC_002947/4793765‑4794057 | gATCGGCAACAGCGCGAAATCGACGCCCTCCCCGCGCATGGCATCCAGAAACAACCGTCGCTTGGCAGCGGGCAGCTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCgg > 2:485511/1‑150 (MQ=255) gcgAAATCGACGCCCTCCCCGCGCATGGCATCCAGAAACAACCGTCGCTTGGCAGCGGGCAGCTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCaaa > 1:608673/1‑150 (MQ=255) cgAAATCGACGCCCTCCCCGCGCATGGCATCCAGAAACAACCGTCGCTTGGCAGCGGGCAGCTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCATGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCaacg > 2:549028/1‑148 (MQ=255) gAAATCGACTCCCTCCCCGCGCATGGCATCCAGAAACAACCGTCGCTTGGCAGCGGGCAGCTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGc > 2:1063054/1‑150 (MQ=255) gAAATCGACGCCCTCCCCGCGCATGGCATCCAGAAACAACCGTCGCTTGGCAGCGGGCAGCTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGc < 2:892780/150‑1 (MQ=255) tccaCGCCCTCCCCGCGCTTGGCATCCAGAATCAACCGTCGCTTTGCAGCGGGCAGCTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCgctc < 1:49756/147‑4 (MQ=255) cGCCCTCCCCGCGCATGGCATCCAGAAACAACCGTCGCTTGGCAGCGGGCAGCTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAg < 2:128754/150‑1 (MQ=255) gCCCTCCCCGCGCATGGCATCCAGAAACAACCGTCGCTTGGCAGCGGGCAGCTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGt > 1:116539/1‑150 (MQ=255) cccTCCCCGCGCATGGCATCCAGAAACAACCGTCGCTTGGCAGCGGGCAGCTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTa < 2:1204178/150‑1 (MQ=255) ccTCCCCGCGCATGGCATCCAGAAACAAGCGGCTCTTGGCAGCGGGCAGCTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAg > 2:1235812/1‑150 (MQ=255) ccTCCCCGCGCATGGCATCCAGAAACAACCGTCGCTTGGCAGCGGGCAGCTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAg > 2:1165385/1‑150 (MQ=255) ccccGCGCATGGCATCCAGAAACAACCGTCGCTTGGCAGCGGGCAGCTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAAc > 2:986393/1‑150 (MQ=255) ccGCGCATGGCATCCAGAAACAACCGTCGCTTGGCAGCGGGCAGCTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGa > 2:288067/1‑150 (MQ=255) gcgcATGGCATCCAGAAACAACCGTCGCTTGGCAGCGGGCAGCTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGc < 1:986393/150‑1 (MQ=255) aTGGCATCCAGAAACAACTGTCGCTTGGCAGCGGGCTGCTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCggag < 2:1351181/150‑1 (MQ=255) gCATCCAGAAACAACCGTCGCTTGGCAGCGGGCAGCTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCggaggga < 2:529948/150‑1 (MQ=255) tCCAGAAACAACCGTCGCTTGGCAGCGGGCAGCTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCgg > 2:1226699/1‑115 (MQ=255) tCCAGAAACAACCGTCGCTTGGCAGCGGGCAGCTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCgg < 1:1226699/115‑1 (MQ=255) ccAGAAACAACCGTCGCTTGGCAGCGGGCAGCTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGc > 1:1087421/1‑150 (MQ=255) gAAACAACCGTCGCTTGGCAGCGGGCAGCTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGaa < 2:1087421/150‑1 (MQ=255) aaaCAACCGTCGCTTGGCAGCGGGCAGCTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGc < 1:189382/111‑1 (MQ=255) aaaCAACCGTCGCTTGGCAGCGGGCAGCTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGc > 2:189382/1‑111 (MQ=255) aaaCAACCGTCGCTTGGCAGCGGGCAGCTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGagggaggg < 1:819478/145‑1 (MQ=255) aaaCAACCGTCGCTTGGCAGCGGGCAGCTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGagggaggg > 2:819478/1‑145 (MQ=255) aacaacCGTCGCTTGGCAGCGGGCAGCTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAAtt < 1:288067/150‑1 (MQ=255) ccGTCGCTTGGCAGCGGGCAGCTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAAcg < 1:485511/150‑1 (MQ=255) tCGCTTGGCAGCGGGCAGCTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCgg < 2:595169/150‑1 (MQ=255) gCTTGGCAGCGGGCAGCACGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGAGAGGGCGAATTTAACGCGGCa > 2:730630/1‑150 (MQ=255) cTTGGCAGCGGGCAGCTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAg > 2:1283063/1‑150 (MQ=255) gCGGGCAGCTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATg > 1:908884/1‑150 (MQ=255) cTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCAt < 2:1359989/141‑1 (MQ=255) cTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCAt > 1:1359989/1‑141 (MQ=255) gAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGAGCCATGCCCGGACGCTGCGCTGGCGAgc < 2:837450/150‑1 (MQ=255) gTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGc > 1:899913/1‑150 (MQ=255) tgagtCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCaa < 1:201666/148‑1 (MQ=255) tgagtCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCaa > 2:201666/1‑148 (MQ=255) agtCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTc < 1:311252/66‑1 (MQ=255) agtCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTc > 2:311252/1‑66 (MQ=255) tCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCCGCa > 2:700558/1‑150 (MQ=255) ttGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCggag < 2:642220/79‑1 (MQ=255) ttGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCggag > 1:642220/1‑79 (MQ=255) cATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGTGCTTGCGGCTTGCCGCAGGCGCTCGCca < 1:1165385/150‑1 (MQ=38) cTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCCGCAAGCACTCGCCACAGt > 1:1047358/1‑150 (MQ=255) ggggTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCCGCAAGCACTCGCCACAGTGGc < 1:1235812/150‑1 (MQ=255) ggTCCTTACTGATCTGCAGCTTGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAgcgc > 2:22630/1‑115 (MQ=255) ggTCCTTACTGATCTGCAGCTTGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAgcgc > 2:22549/1‑115 (MQ=255) ggTCCTTACTGATCTGCAGCTTGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAgcgc < 1:22630/115‑1 (MQ=255) ggTCCTTACTGATCTGCAGCTTGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAgcgc < 1:22549/115‑1 (MQ=255) gTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGACATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCCGCAAGCACTCGCCACAGTGGCCTc > 1:1335910/1‑150 (MQ=255) tCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAgcgc < 2:1380517/113‑1 (MQ=255) tCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAgcgc > 1:1380517/1‑113 (MQ=255) cTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCCGCAAGCACTCGCCACAGTGGCCTCAAAgcgc > 2:55351/1‑150 (MQ=255) tGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCCGCAAGCACTCGCCACAGTGGCCTCAAAGcgcc > 2:1074479/1‑150 (MQ=255) gCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCCGCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTcg > 2:429720/1‑150 (MQ=255) gCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCCGCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTcg > 1:1082978/1‑150 (MQ=255) gCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTCGCCTTAAGATCGGTCAAGCCGCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTcc > 2:37257/1‑149 (MQ=255) aGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCCGCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTcgcg < 1:700558/150‑1 (MQ=255) aGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCCGCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTcgcg < 1:244950/150‑1 (MQ=255) | GATCGGCAACAGCGCGAAATCGACGCCCTCCCCGCGCATGGCATCCAGAAACAACCGTCGCTTGGCAGCGGGCAGCTCGATGAAGCGTCGAGCCAGTTTGAGTGAGTCTTGGGCGTTCATGCTCGGGGTCCTTACTGATCTGCAGCTCGGCCGTGCGGCCAAAGCGTTTCCAGTAGAACGAGCGGAGGGAGGGCGAATTTAACGCGGCAGCGCCATGCCCGGACGCTGCGCTGGCGAGCGCCTGCGGCAAGCCGCAAGCACTCGCCACAGTGGCCTCAAAGCGCCGCTTCGCG > NC_002947/4793765‑4794057 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |