Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 432,996 A→T 8.9% intergenic (+18/+29) PP_0355 → / ← glcB two‑component system response regulator/malate synthase G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947432,9960AT 8.9% 170.1 / 7.5 56intergenic (+18/+29)PP_0355/glcBtwo‑component system response regulator/malate synthase G
Reads supporting (aligned to +/- strand):  ref base A (25/26);  new base T (3/2);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.03e-01

GTGTCGGGCGACACCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTGGAACGCCA  >  NC_002947/432850‑433140
                                                                                                                                                   |                                                                                                                                                
gtgtCGGGCGACACCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTaaaa                                                                                                                                               <  2:436056/150‑1 (MQ=255)
 tgtCGGGCGACACCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAAc                                                                                                                                              >  1:942329/1‑150 (MQ=255)
       gCGACACCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTccc                                                                                                                                        >  1:573273/1‑150 (MQ=255)
       gCGACACCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTccc                                                                                                                                        >  1:1353966/1‑150 (MQ=255)
        cGACACCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTcccc                                                                                                                                       <  2:150493/150‑1 (MQ=255)
        cGACACCGATGTGGAAAGGGCAGTGTTTTTGAGGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTTTTACGGTcccc                                                                                                                                       <  1:314478/150‑1 (MQ=255)
          acacCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCt                                                                                                                                     >  1:475989/1‑150 (MQ=255)
              cGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCTGCCAGGGGGACCGTTTTACGGTcccc                                                                                                                                       >  1:618441/1‑145 (MQ=255)
              cGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCTGCCAGGGGGACCGTTTTACGGTcccc                                                                                                                                       <  2:618441/145‑1 (MQ=255)
                 tgtgGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAATAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGgcagca                                                                                                                              <  2:125851/150‑1 (MQ=255)
                   tgGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGGTGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGa                                                                                                                            <  1:271164/150‑1 (MQ=255)
                     gAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGAtg                                                                                                                          >  2:778593/1‑150 (MQ=255)
                           gCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCtt                                                                                                                    >  1:1297641/1‑150 (MQ=255)
                               tGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGGCCGGTGTAGGACGTGGGGAGCAAAGAACTGAAAATTGGATGACGC‑GCAAGGGCCATTGTATATCGGTCCCCCTGGCAGCAGATGCTGCTTACaa                                                                                                                <  1:1390993/150‑1 (MQ=255)
                                  aTGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTCAACAAAGAACTGAAAATTGGATGCTGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAAccc                                                                                                             >  1:1029984/1‑150 (MQ=255)
                                    gtgATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGt                                                                                                           <  1:1035858/150‑1 (MQ=255)
                                      gATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTa                                                                                                         <  2:1353966/150‑1 (MQ=255)
                                       aTGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTAc                                                                                                        >  2:1335455/1‑150 (MQ=255)
                                             cTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTcccc                                                                                                                                       <  2:879689/113‑1 (MQ=255)
                                             cTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTcccc                                                                                                                                       >  1:879689/1‑113 (MQ=255)
                                             cTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCt                                                                                                  <  2:942329/150‑1 (MQ=255)
                                              tGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCtt                                                                                                 <  2:475989/150‑1 (MQ=255)
                                              tGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCtt                                                                                                 <  1:5724/150‑1 (MQ=255)
                                                     ggtggACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTcg                                                                                          <  1:778593/150‑1 (MQ=255)
                                                      gtggACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTcgc                                                                                         >  2:611665/1‑150 (MQ=255)
                                                       tggACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTTCTTACAACCCGTTACGCGCCTTGAACTcgcg                                                                                        <  1:478595/150‑1 (MQ=255)
                                                             tCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCACTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTcgc                                                                                         <  2:483682/143‑1 (MQ=255)
                                                             tCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCACTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTcgc                                                                                         >  1:483682/1‑143 (MQ=255)
                                                                tGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGAcggcg                                                                               <  2:573273/150‑1 (MQ=255)
                                                                  cTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGt                                                                             >  2:577825/1‑150 (MQ=255)
                                                                  cTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGt                                                                             >  2:1111514/1‑150 (MQ=255)
                                                                   tCAAGCCGGGGGATCGGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAAGTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTAGCGGCGACGGCtgtg                                                                            >  2:35966/1‑150 (MQ=255)
                                                                      aGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAg                                                                         <  1:577825/150‑1 (MQ=255)
                                                                          ggTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTAcc                                                                     <  1:1111514/150‑1 (MQ=255)
                                                                            tGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAAAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCgg                                                                   <  1:178671/150‑1 (MQ=255)
                                                                                tCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCg                                                               >  1:910212/1‑150 (MQ=255)
                                                                                  tGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGt                                                             <  1:855329/150‑1 (MQ=255)
                                                                                         gCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGtt                                                                                                          >  2:628219/1‑98 (MQ=255)
                                                                                         gCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGtt                                                                                                          <  1:628219/98‑1 (MQ=255)
                                                                                         gCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCg                                                      >  1:1296588/1‑150 (MQ=255)
                                                                                                      gTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTcccc                                                                                                                                       >  1:103743/1‑56 (MQ=255)
                                                                                                      gTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTcccc                                                                                                                                       <  2:103743/56‑1 (MQ=255)
                                                                                                      gTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCATACACCCCGTTACGCTCCTTGAACTCTCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGttgcttgc                                         <  1:174840/150‑1 (MQ=255)
                                                                                                            aaaGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGATCGGTATAACCGGACGGTTGCTGTCCAccgt                                   >  2:792901/1‑148 (MQ=255)
                                                                                                                   tGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTAc                            <  2:884193/150‑1 (MQ=255)
                                                                                                                   tGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTAc                            <  2:884200/150‑1 (MQ=255)
                                                                                                                         ttGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTc                      <  2:1297641/150‑1 (MQ=255)
                                                                                                                               tgaagtgcCAGAGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAg                 <  1:1059215/144‑1 (MQ=255)
                                                                                                                                     gcCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCt           >  2:215194/1‑150 (MQ=255)
                                                                                                                                     gcCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCAGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCt           >  2:700482/1‑150 (MQ=255)
                                                                                                                                        aGGGGGACCGTTTTACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTGGa        >  2:470156/1‑150 (MQ=255)
                                                                                                                                        aGGGGGACCGTTTTACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCAGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTggg        >  1:162985/1‑149 (MQ=255)
                                                                                                                                        aGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCa                                       >  2:1307562/1‑119 (MQ=255)
                                                                                                                                        aGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCa                                       <  1:1307562/119‑1 (MQ=255)
                                                                                                                                          ggggACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTGAAAc      >  2:366504/1‑150 (MQ=255)
                                                                                                                                           gggACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTGGAACg     >  2:1331332/1‑150 (MQ=255)
                                                                                                                                              aCCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTGGAACGCCa  >  2:254190/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GTGTCGGGCGACACCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTGGAACGCCA  >  NC_002947/432850‑433140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑