Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,858,086 G→C 6.0% G31A (GGG→GCG)  PP_4273 → MerR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,858,0860GC 6.0% 212.6 / 4.5 67G31A (GGG→GCG) PP_4273MerR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (33/30);  new base C (2/2);  total (35/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.78e-01

TCATTACCATGCCGCGCAGCATAGAAGTTGAAGTCAACTTCAAGTCAAGGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGG  >  NC_002947/4857938‑4858232
                                                                                                                                                    |                                                                                                                                                  
tCATTACCATGCCGCGCAGCATAGAAGTTGAAGTCAACTTCAAGTCAAGGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCggg                                                                                                                                                   >  1:817944/1‑150 (MQ=255)
           ccGCGCAGCATAGAAGTTGAAGTCAACTTCAAGTCAAGGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGCGCCCGGCCaagg                                                                                                                                        <  1:774272/150‑4 (MQ=255)
             gcgcAGCATAGAAGTTGAAGTCAACTTCAAGTCAAGGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTc                                                                                                                                      <  2:817944/150‑1 (MQ=255)
                     ttgAAGTTGCCTTCAACTTCAAGTCAAGGGCCCGAGATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGttt                                                                                                                              >  2:59822/3‑150 (MQ=255)
                      aGAAGTTGAAGTCAACTTCAAGTCAAGGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTg                                                                                                                             >  1:45114/1‑150 (MQ=255)
                             gAAGTCAACTTCAAGTCAAGGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGa                                                                                                                      <  1:361757/150‑1 (MQ=255)
                                ttCAACTTCAAGTCAAGGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGAtgt                                                                                                                   <  1:59822/149‑1 (MQ=255)
                                gTCAACTTCAAGTCAAGGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGAtgt                                                                                                                   <  2:917378/150‑1 (MQ=255)
                                 tCAACTTCAAGTCAAGGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGAtgtg                                                                                                                  >  1:1148952/1‑150 (MQ=255)
                                 tCAACTTCAAGTCAAGGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGACCAGCGTCGACAGTTTGCAGCCGAtgtg                                                                                                                  >  1:1202041/1‑150 (MQ=255)
                                      ttCAAGTCAAGGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGa                                                                                                             >  1:1004432/1‑150 (MQ=255)
                                      ttCAAGTCAAGGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGa                                                                                                             >  1:1149435/1‑150 (MQ=255)
                                       tcaagtcaagGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGAc                                                                                                            >  1:22805/1‑150 (MQ=255)
                                               agGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGc                                                                                                                         <  2:1389656/129‑1 (MQ=255)
                                               agGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGc                                                                                                                         >  1:1389656/1‑129 (MQ=255)
                                               agGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGgc                                                                                                    <  2:45114/150‑1 (MQ=255)
                                                  gCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCt                                                                                                 <  2:1004432/150‑1 (MQ=255)
                                                   cccGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTg                                                                                                >  2:1075208/1‑150 (MQ=255)
                                                     cGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACGGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGAt                                                                                              >  2:246876/1‑150 (MQ=255)
                                                      gAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATc                                                                                             >  2:1243451/1‑150 (MQ=255)
                                                                         tCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCgg                                                                          >  2:1222366/1‑150 (MQ=255)
                                                                         tCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCgg                                                                          <  1:403666/150‑1 (MQ=255)
                                                                          cGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCggg                                                                         <  1:1075208/150‑1 (MQ=255)
                                                                              aaaCGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGttt                                                                     >  2:628314/1‑150 (MQ=255)
                                                                               aaCGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGtttt                                                                    <  2:1148952/150‑1 (MQ=255)
                                                                                    aCTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTg                                                               >  2:392610/1‑150 (MQ=255)
                                                                                    aCTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTg                                                               >  2:626464/1‑150 (MQ=255)
                                                                                       ggCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGACCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGAt                                                            <  2:1202041/150‑1 (MQ=255)
                                                                                           tACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAg                                                        <  1:252450/150‑1 (MQ=255)
                                                                                              cTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTg                                                                                                                             >  1:693408/1‑78 (MQ=255)
                                                                                              cTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTg                                                                                                                             <  2:693408/78‑1 (MQ=255)
                                                                                              cTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTgg                                                     <  1:392610/150‑1 (MQ=255)
                                                                                                gCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTgggg                                                   >  2:496909/1‑150 (MQ=255)
                                                                                                    cGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTgggg                                                   <  1:1238409/146‑1 (MQ=255)
                                                                                                    cGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTgggg                                                   >  2:1238409/1‑146 (MQ=255)
                                                                                                             gTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCtggtgg                                      >  1:213915/1‑150 (MQ=255)
                                                                                                              ttACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGa                                                                                                                      <  1:1179009/69‑1 (MQ=255)
                                                                                                              ttACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGa                                                                                                                      >  2:1179009/1‑69 (MQ=255)
                                                                                                               tactacGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGAc                                    <  1:236083/150‑1 (MQ=255)
                                                                                                               tactacGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGAc                                    <  1:1243451/150‑1 (MQ=255)
                                                                                                                actacGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGAcc                                   <  2:22805/150‑1 (MQ=255)
                                                                                                                    cGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGTAAACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCa                               <  1:246876/150‑1 (MQ=255)
                                                                                                                     gagaAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGTCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAg                              >  2:134323/1‑150 (MQ=255)
                                                                                                                     gagaAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAg                              >  1:419219/1‑150 (MQ=255)
                                                                                                                      agaagaAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAgg                             >  1:569842/1‑150 (MQ=255)
                                                                                                                      agaagaAAGGCGTGCTCAAGTCCTTGGGCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGAGCGGTTGGCGCTGATCGCGCTGGGGCAGGCCGCGGGGTTTTCGCGGGAGGAAGTGGGGGCGATGCTGGTGGACCTCCAgg                             >  2:410898/1‑150 (MQ=255)
                                                                                                                          gaaAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGAt                                                            >  2:437473/1‑115 (MQ=255)
                                                                                                                          gaaAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGAt                                                            <  1:437473/115‑1 (MQ=255)
                                                                                                                          gaaAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTggg                                                    >  2:790236/1‑123 (MQ=255)
                                                                                                                          gaaAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTggg                                                    <  1:790236/123‑1 (MQ=255)
                                                                                                                          gaaAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGa                         >  2:1153113/1‑150 (MQ=255)
                                                                                                                          gaaAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGa                         >  2:932062/1‑150 (MQ=255)
                                                                                                                                  tGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGa                 <  1:895134/150‑1 (MQ=255)
                                                                                                                                   gCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGAt                >  1:1264956/1‑150 (MQ=255)
                                                                                                                                   gCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGAt                >  1:405940/1‑150 (MQ=255)
                                                                                                                                     tCAAGTCCTTGGCCGGGCGCGGCCAGCGACGACAGGTTGCAGCCGATGTGGCAGACCGGTGGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGc              <  1:1366650/150‑1 (MQ=255)
                                                                                                                                       aaGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGACGTGGGGGCGATGCGGGTGCGCCTGCACATCGACCAGCAGATGCTg            >  2:1374654/1‑150 (MQ=255)
                                                                                                                                       aaGTCCTAGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGAGGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTGGACCGGCAGATGCTg            >  1:765977/1‑150 (MQ=255)
                                                                                                                                        aGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGa           <  2:419219/150‑1 (MQ=255)
                                                                                                                                         gacgctGGCCGCGCCCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGAt          >  2:119979/6‑150 (MQ=255)
                                                                                                                                         gTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGAt          <  2:569842/150‑1 (MQ=255)
                                                                                                                                         gTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGAt          <  2:213915/150‑1 (MQ=255)
                                                                                                                                              tGGCCGCGCCCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCtggtg                                       >  2:285620/1‑116 (MQ=255)
                                                                                                                                              tGGCCGCGCCCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCtggtg                                       <  1:285620/116‑1 (MQ=255)
                                                                                                                                               ggCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCaa    <  1:154777/150‑1 (MQ=255)
                                                                                                                                                 ccGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAgg  <  2:1149435/150‑1 (MQ=255)
                                                                                                                                                  cGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCggg                                                                         >  2:1395335/1‑78 (MQ=255)
                                                                                                                                                  cGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCggg                                                                         <  1:1395335/78‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TCATTACCATGCCGCGCAGCATAGAAGTTGAAGTCAACTTCAAGTCAAGGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGG  >  NC_002947/4857938‑4858232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑