Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,860,858 G→C 5.1% L79L (CTC→CTG)  ligA ← DNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,860,8580GC 5.1% 243.1 / 4.2 79L79L (CTC→CTG) ligADNA ligase
Reads supporting (aligned to +/- strand):  ref base G (38/37);  new base C (2/2);  total (40/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.59e-01

GCCGTCGCGGTACAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTCGGCATCAGG  >  NC_002947/4860714‑4861007
                                                                                                                                                |                                                                                                                                                     
gCCGTCGCGGTACAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCTCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCAt                                                                                                                                                  >  1:1008368/1‑150 (MQ=255)
  cGTCGCGGTACAGCAGGCTCACGGCCAGGCCATCCAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGACTGATCGAGGCCCTCTACCACGCTACGGCCGAACTCACGCAGGGCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATgg                                                                                                                                                >  2:250050/1‑150 (MQ=255)
   gTCGCGGTACAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATgtg                                                                                                                                               <  2:167030/150‑3 (MQ=255)
           aCAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGt                                                                                                                                       <  2:241938/150‑1 (MQ=255)
            cagcagGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTg                                                                                                                                      <  2:184412/150‑1 (MQ=255)
              gcagGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTg                                                                                                                                      >  1:330217/1‑148 (MQ=255)
              gcagGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTg                                                                                                                                      <  2:330217/148‑1 (MQ=255)
              gcagGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGc                                                                                                                                    <  1:611139/150‑1 (MQ=255)
              gcagGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATAGAGGCCTCCTACCGCGCCCCAGCCGTCACCCTGCACCTCCACTTATCGGAATGCGTTGCCCATACTGATCATGGGCACTTCGTGGc                                                                                                                                    <  2:374690/150‑1 (MQ=255)
                agGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGt                                                                                                                                  >  1:1070798/1‑150 (MQ=255)
                    tCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGCCGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCt                                                                                                                              >  1:1162031/1‑150 (MQ=255)
                      ccGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTgg                                                                                                                            <  2:214245/149‑1 (MQ=255)
                              gccaTCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGc                                                                                                                           <  2:122002/143‑1 (MQ=255)
                              gccaTCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGc                                                                                                                           >  1:122002/1‑143 (MQ=255)
                              gccaTCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAAcgc                                                                                                                    >  2:715601/1‑150 (MQ=255)
                              gccaTCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAAcgc                                                                                                                    >  1:218440/1‑150 (MQ=255)
                                  tCGGGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACgccgcc                                                                                                                >  2:591761/1‑150 (MQ=255)
                                    gAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCaa                                                                                                              <  2:1070798/150‑1 (MQ=255)
                                    gAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCaa                                                                                                              >  1:359049/1‑150 (MQ=255)
                                    gAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGATTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCaa                                                                                                              <  1:112768/150‑1 (MQ=255)
                                            gTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATg                                                                                                                                                 <  1:965603/107‑1 (MQ=255)
                                            gTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATg                                                                                                                                                 >  2:965603/1‑107 (MQ=255)
                                            gTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTc                                                                                                      >  2:134645/1‑150 (MQ=255)
                                                gCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATggg                                                                                                                                               <  1:566251/105‑1 (MQ=255)
                                                gCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATggg                                                                                                                                               >  2:566251/1‑105 (MQ=255)
                                                   gCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTccacccac                                                                                               >  1:241823/1‑150 (MQ=255)
                                                                gcgcCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCAGGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGc                                                                                  <  1:31718/150‑1 (MQ=255)
                                                                       gCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCgg                                                                           <  1:464875/150‑1 (MQ=255)
                                                                         tGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCgggg                                                                         <  2:218440/150‑1 (MQ=255)
                                                                              gAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAAGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTAc                                                                    >  2:283887/1‑150 (MQ=255)
                                                                                     tCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGc                                                             <  2:166434/150‑1 (MQ=255)
                                                                                       tACCACGCGACGGCCGAACTCAAGCCGGTCGGTTTCTTCGAAGGTGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCgg                                                           <  2:1008368/150‑1 (MQ=255)
                                                                                        aCCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCggg                                                          >  1:1100351/1‑150 (MQ=255)
                                                                                        aCCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCggg                                                          >  2:1061093/1‑150 (MQ=255)
                                                                                         ccACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCATGCTCAGCCTGGGCaac                                                                                                                                           >  2:492888/1‑66 (MQ=37)
                                                                                         ccACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCATGCTCAGCCTGGGCaac                                                                                                                                           <  1:492888/68‑3 (MQ=37)
                                                                                          cACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGTGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGgtg                                                        >  2:618975/1‑150 (MQ=255)
                                                                                           aCGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCgg                                                           >  1:849572/1‑146 (MQ=255)
                                                                                           aCGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCgg                                                           <  2:849572/146‑1 (MQ=255)
                                                                                           aCGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGgtgt                                                       >  1:1229852/1‑150 (MQ=255)
                                                                                              cgACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCa                                                    <  1:1115448/150‑1 (MQ=255)
                                                                                               gACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAg                                                   >  1:1039417/1‑150 (MQ=255)
                                                                                                   gCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGa                                                                                <  2:1312824/117‑1 (MQ=255)
                                                                                                   gCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGa                                                                                >  1:1312824/1‑117 (MQ=255)
                                                                                                      gAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGCTCAGCTTCCAg                                            <  2:1100351/150‑1 (MQ=255)
                                                                                                       aaCTCATGCATGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAgc                                           <  1:421227/150‑1 (MQ=255)
                                                                                                        aCTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAgcg                                          >  1:1204020/1‑150 (MQ=255)
                                                                                                        aCTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAgcg                                          >  1:235676/1‑150 (MQ=255)
                                                                                                         cTCACGCAGGGCGGCTTCTTCGAAGGCGTTGCCCAGGCTGTGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAgcgc                                         <  1:618975/150‑1 (MQ=255)
                                                                                                          tCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCa                                                    <  2:1148291/138‑1 (MQ=255)
                                                                                                          tCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCa                                                    >  1:1148291/1‑138 (MQ=255)
                                                                                                              gCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGa                                    <  2:592028/150‑1 (MQ=255)
                                                                                                              gCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGa                                    >  1:592028/1‑150 (MQ=255)
                                                                                                                   tCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCCGGTGCGGGTGTTGAGCTTCCAGGGCCTTGAGATCg                               >  1:198587/1‑150 (MQ=255)
                                                                                                                    cGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGt                              <  1:1061093/150‑1 (MQ=255)
                                                                                                                    cGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGt                              <  2:1162031/150‑1 (MQ=255)
                                                                                                                       cttcttGGAGGGGGTTGCCGAGGCTGATCAGGGGGGCTTGGTGGCGTAGCTGGCTGAAGGCCGCCAAGGCAGCTCCACCCACACGCTGGGCGGGCCGGTCGGGGGGTACCACGTGCGGCGGTTGAGCTTCCAGCGCCTTGAGCTCGTGGa                           >  2:798386/1‑150 (MQ=255)
                                                                                                                       cttcttCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGa                           >  2:1346269/1‑150 (MQ=255)
                                                                                                                        ttcttcGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGa                                                                                <  1:373449/96‑1 (MQ=255)
                                                                                                                        ttcttcGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGa                                                                                >  2:373449/1‑96 (MQ=255)
                                                                                                                        ttcttcGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAg                                                   <  1:618825/125‑1 (MQ=255)
                                                                                                                        ttcttcGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAg                                                   >  2:618825/1‑125 (MQ=255)
                                                                                                                          cttcGAAGGCGTTGCCCAGGCTGCGCATGGGCACTTCGTGGCGTAGCTGGCTGTACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGTTGCGGGTGTTCAGCTTCCAGCTCCTTGAGCTCGTTGAACa                        <  2:326477/150‑1 (MQ=255)
                                                                                                                          cttcGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCgg                                                                           >  2:1370017/1‑99 (MQ=255)
                                                                                                                          cttcGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCgg                                                                           <  1:1370017/99‑1 (MQ=255)
                                                                                                                          cttcGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACa                        <  2:1229852/150‑1 (MQ=255)
                                                                                                                           ttcGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGt                                                                              <  2:1101293/95‑1 (MQ=255)
                                                                                                                           ttcGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGt                                                                              >  1:1101293/1‑95 (MQ=255)
                                                                                                                             cGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGc                     <  2:1364367/150‑1 (MQ=255)
                                                                                                                                      tGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCTAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTAtt            <  2:241823/150‑1 (MQ=255)
                                                                                                                                        cccATGCTCAGCCTGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAgg                                                                                                            <  1:920090/52‑1 (MQ=25)
                                                                                                                                        cccATGCTCAGCCTGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAgg                                                                                                            >  2:920090/1‑52 (MQ=25)
                                                                                                                                         ccAGGCTGAGCATGGGCACTTCGTGGCGTTTCGGGCTGAACGCCGCCAAGGCAGCTCCACCCGCACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCCGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTCgg         >  1:398087/1‑150 (MQ=255)
                                                                                                                                         ccAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCtg                                                                                         >  2:1142223/1‑70 (MQ=255)
                                                                                                                                         ccAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCtg                                                                                         <  1:1142223/70‑1 (MQ=255)
                                                                                                                                         ccAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTCgg         >  2:682080/1‑150 (MQ=255)
                                                                                                                                          cAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTCGGc        >  2:628878/1‑150 (MQ=255)
                                                                                                                                              cTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTCGGc        >  2:46520/1‑146 (MQ=255)
                                                                                                                                              cTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTCGGc        <  1:46520/146‑1 (MQ=255)
                                                                                                                                                gAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTCGGCATCAgg  <  2:235676/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
GCCGTCGCGGTACAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTCGGCATCAGG  >  NC_002947/4860714‑4861007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑