| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,900,083 | G→T | 7.4% | *146L (TGA→TTA) | PP_4308 → | AsnC family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,900,083 | 0 | G | T | 7.4% | 164.4 / 2.9 | 55 | *146L (TGA→TTA) | PP_4308 | AsnC family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (27/23); new base T (2/2); total (29/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.59e-01 | |||||||||||
TTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACAC > NC_002947/4899936‑4900227 | ttGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGgattgat < 1:1074044/150‑1 (MQ=255) tGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGAGATCATCAAGAAGACCCAGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTAAtgtg > 1:13640/1‑150 (MQ=255) ttCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTg > 1:1413220/1‑150 (MQ=255) tcgCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTgg > 2:41402/1‑135 (MQ=255) tcgCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTgg < 1:41402/135‑1 (MQ=255) cTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAg < 1:786461/150‑1 (MQ=255) tGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAgg > 2:515191/1‑150 (MQ=255) cGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGaa < 2:1413220/150‑1 (MQ=255) gAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGc > 1:1115683/1‑150 (MQ=255) gcagcTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCa > 2:248704/1‑150 (MQ=255) gACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCt > 2:149291/1‑102 (MQ=255) gACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCt < 1:149291/102‑1 (MQ=255) ccTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGgattgatt > 2:18525/1‑93 (MQ=255) ccTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGgattgatt < 1:18525/93‑1 (MQ=255) ccTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGgattga < 2:252337/91‑1 (MQ=255) ccTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGgattga > 1:252337/1‑91 (MQ=255) ccTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGc > 2:912282/1‑136 (MQ=255) ccTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGc < 1:912282/136‑1 (MQ=255) cTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTTATTGGCTCCTGTACTGGCCTCTTCGCGGGCACGCCTGCGAagag > 2:211660/1‑133 (MQ=38) cTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTTATTGGCTCCTGTACTGGCCTCTTCGCGGGCACGCCTGCGAagag > 2:211591/1‑133 (MQ=38) cTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTTATTGGCTCCTGTACTGGCCTCTTCGCGGGCACGCCTGCGAagag < 1:211660/133‑1 (MQ=38) cTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTTATTGGCTCCTGTACTGGCCTCTTCGCGGGCACGCCTGCGAagag < 1:211591/133‑1 (MQ=38) cTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGc > 2:571591/1‑103 (MQ=255) cTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGc < 1:571591/103‑1 (MQ=255) gACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCGGGCACGCCTGCGAAGAGGCCAGCACAGGa < 1:838288/139‑1 (MQ=37) gACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCGGGCACGCCTGCGAAGAGGCCAGCACAGGa > 2:838288/1‑139 (MQ=37) aCCTCATGAATGTGCTGGCCGAGACCAATTCCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTCATTGGCTCCtgtg < 2:1023476/95‑1 (MQ=255) aCCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCtgtg > 1:1023476/1‑95 (MQ=255) cTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGc > 1:1233460/1‑98 (MQ=255) cTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGc < 2:1233460/98‑1 (MQ=255) cTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTAttt > 2:592079/1‑150 (MQ=255) tCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGACACGGAAAGTACAGGAAAAGCTGAGTAtttt > 1:1022864/1‑150 (MQ=255) tCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTAtttt > 1:1420959/1‑150 (MQ=255) aTGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTAtttttt > 1:1062907/1‑150 (MQ=255) cTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACaaa > 2:1148143/1‑150 (MQ=255) gCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTAtttttt > 1:1240969/1‑138 (MQ=255) gCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTAtttttt < 2:1240969/138‑1 (MQ=255) agaCCAATACCGCGATCATCAAGAAGCCCCCGGTCAAGCGGTGGTTGCCACCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTaa < 2:970180/150‑1 (MQ=255) agaCCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTaa < 1:515191/150‑1 (MQ=255) cAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCAt > 2:1203929/1‑150 (MQ=255) aCCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGt < 1:248704/150‑1 (MQ=255) ccGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGGATTTTTTGTACACAAAAACGTAACCATAGGTg > 2:113213/1‑150 (MQ=255) gATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCAt > 1:163260/1‑150 (MQ=255) atcatcAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCAtt > 2:600876/1‑150 (MQ=255) tatcaAAAATACTCCGGTCGAGCGGCGGTTGCCGCCAATGGATTGTTTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCAAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCAtttt < 1:303101/149‑1 (MQ=255) caAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACCAAAACGTAACCATAGGTGCCATTTTtgt < 1:592079/150‑1 (MQ=255) caAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTtgt < 2:1082711/150‑1 (MQ=255) agaagaCCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTtgtgt < 1:113213/150‑1 (MQ=255) agaagaCCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTtgtgt < 1:600876/150‑1 (MQ=255) aagaCCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGc < 1:1148143/150‑1 (MQ=255) cccGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGt < 1:1052360/108‑1 (MQ=255) cccGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGt > 2:1052360/1‑108 (MQ=255) cccGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACttt > 1:856342/1‑150 (MQ=255) cccGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACttt > 1:220406/1‑150 (MQ=255) gTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTttcttc < 2:1115683/150‑1 (MQ=255) gcggcgGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAACACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTgg > 1:304246/1‑150 (MQ=255) ccAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGt < 1:1203929/150‑1 (MQ=255) aaTGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTa < 2:1420959/150‑1 (MQ=255) ggATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTacac < 2:163260/150‑1 (MQ=255) ggATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTacac > 2:1393885/1‑150 (MQ=255) attgattgGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTac < 1:951330/81‑1 (MQ=255) attgattgGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTac > 2:951330/1‑81 (MQ=255) | TTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACAC > NC_002947/4899936‑4900227 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |