| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 58,731 | A→G | 10.9% | intergenic (‑642/+46) | PP_5424 ← / ← PP_0050 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 58,731 | 0 | A | G | 10.9% | 117.5 / 5.0 | 46 | intergenic (‑642/+46) | PP_5424/PP_0050 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (21/20); new base G (2/3); total (23/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01 | |||||||||||
TTTTGTGATTGGTGAGTAGGGATTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGG > NC_002947/58590‑58872 | ttttGTGATTGGTGAGTAGGGATTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTaaaa > 1:24252/1‑150 (MQ=255) tttGTGATTGGTGAGTAGGGATTTCTCGGCTTTTCTGTGAAAAGCGCCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTaaaaa < 2:1069823/150‑1 (MQ=255) aTTGGTGAGTAGGGATTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCgc > 2:346734/1‑150 (MQ=255) gTAGGGATTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATACGTCAGACACTAAAAAGCCCGCTGCTGCgg < 1:750420/150‑1 (MQ=255) tAGGGATTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGGCACTGAAAAGCCCGCAGCAGCggg > 1:141619/1‑150 (MQ=255) gggATTTCTAGGATTTTCTGTGAAAAGAACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGGCACTAAAAAGCCCGCAGCAGCGGGCt < 2:141619/150‑1 (MQ=255) aTTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGGCACTAAAAAGCCCGCAGCAGCGGGCtttt < 1:366099/150‑1 (MQ=255) ttCTCGGCTTTTCTAAGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTa < 2:1275782/150‑1 (MQ=255) ctcGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGt < 1:1178729/150‑1 (MQ=255) tgAAAAGCACCCCTTCATTTCACAACAAAAATGGCGAAGCTTCCAATTTCACGGCTTCTGGCCTATCACCTATTTTGTGAACCTCCAAGCTGTCATTCGCCCGACACTCCAAATCCCACTACTGCGGGCTTTTTAGTGCCTGCAAACCAt > 2:185629/1‑150 (MQ=255) tgAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCAGCAGCGGGCtt < 2:681952/131‑1 (MQ=255) tgAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCAGCAGCGGGCtt > 1:681952/1‑131 (MQ=255) cACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGc < 2:24252/150‑1 (MQ=255) aTTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGaa < 1:346734/150‑1 (MQ=255) tttCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAAt < 1:185629/150‑1 (MQ=255) acaAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGcc > 2:1293326/1‑119 (MQ=255) acaAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGcc < 1:1293326/119‑1 (MQ=255) aaaaaaaaTGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCAc > 1:1293290/1‑150 (MQ=255) ttCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATg > 2:1114630/1‑128 (MQ=255) ttCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATg < 1:1114630/128‑1 (MQ=255) ccAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAATCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATg < 1:359306/150‑1 (MQ=255) cAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATgg > 1:232761/1‑150 (MQ=255) cACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGccacca < 2:372243/150‑1 (MQ=255) aCGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAg > 1:1383150/1‑150 (MQ=255) gCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTa < 1:862071/75‑1 (MQ=255) gCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTa > 2:862071/1‑75 (MQ=255) gCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACa < 2:1332325/144‑1 (MQ=255) gCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACa > 1:1332325/1‑144 (MQ=255) tATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAg < 2:727388/90‑1 (MQ=255) tATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAg > 1:727388/1‑90 (MQ=255) aCCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTca > 2:1316560/1‑150 (MQ=255) tATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACAt > 2:909804/1‑150 (MQ=255) tATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACAt > 1:1302161/1‑150 (MQ=255) ttttGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATAc > 2:212872/1‑150 (MQ=255) aaCCTACAAGGTGTAATTCGCCAGGCACTAAAAAGCCCGCAGCAGCGGGCtt < 2:1339833/52‑1 (MQ=17) aaCCTACAAGGTGTAATTCGCCAGGCACTAAAAAGCCCGCAGCAGCGGGCtt > 1:1339833/1‑52 (MQ=17) aaCCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATca < 2:29714/99‑1 (MQ=255) aaCCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATca > 1:29714/1‑99 (MQ=255) aaCCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAAc < 2:232761/150‑1 (MQ=255) aGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCa < 2:1383150/150‑1 (MQ=255) tgtAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGa > 1:511331/1‑150 (MQ=255) aaTTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGt < 1:909804/150‑1 (MQ=255) aTTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTg > 2:928999/1‑150 (MQ=255) ttCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTgg > 1:805524/1‑150 (MQ=255) aGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACga < 2:1102656/130‑1 (MQ=255) aGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACga > 1:1102656/1‑130 (MQ=255) | TTTTGTGATTGGTGAGTAGGGATTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGG > NC_002947/58590‑58872 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |