Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,945,333 C→T 9.1% intergenic (+648/+150) PP_5673 → / ← flhB D‑alanine‑‑D‑alanine ligase/flagellin export apparatus substrate specificity protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,945,3330CT 9.1% 165.2 / 5.4 55intergenic (+648/+150)PP_5673/flhBD‑alanine‑‑D‑alanine ligase/flagellin export apparatus substrate specificity protein
Reads supporting (aligned to +/- strand):  ref base C (24/26);  new base T (3/2);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

CTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAG  >  NC_002947/4945184‑4945481
                                                                                                                                                     |                                                                                                                                                    
ctgctgTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTacac                                                                                                                                                      <  1:199564/150‑1 (MQ=255)
 tgctgTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACAcc                                                                                                                                                     >  1:177408/1‑150 (MQ=255)
         aTCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGa                                                                                                                                                 >  1:1067745/1‑146 (MQ=255)
         aTCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGa                                                                                                                                                 <  2:1067745/146‑1 (MQ=255)
                         tGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAg                                                                                                                             >  1:591782/1‑150 (MQ=255)
                         tGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAg                                                                                                                             <  1:871544/150‑1 (MQ=255)
                                        cTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGcc                                                                                                              >  1:1008324/1‑150 (MQ=255)
                                         tgtgAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCg                                                                                                             >  1:1082010/1‑150 (MQ=255)
                                          ttgAGATCGAGCGCCGCCCGCGCGGCGCATCGAGCGCACGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGAGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCgg                                                                                                            <  2:1008324/149‑1 (MQ=255)
                                               aTCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGcaca                                                                                                       <  1:1223605/150‑1 (MQ=255)
                                                tCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACag                                                                                                      <  2:587120/150‑1 (MQ=255)
                                                 gatgcgcCGCGCGGGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACagg                                                                                                     >  2:1002875/4‑150 (MQ=255)
                                                  gAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACagga                                                                                                    <  2:177408/150‑1 (MQ=255)
                                                    gcgcCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACaggagg                                                                                                  <  2:591782/150‑1 (MQ=255)
                                                    gcgcCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACaggagg                                                                                                  <  1:686526/150‑1 (MQ=255)
                                                          cccgcGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGGTTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTgg                                                                                            >  1:759725/1‑150 (MQ=255)
                                                            cgcgcgGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGGTTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGcc                                                                                          <  2:759725/150‑1 (MQ=255)
                                                             gcgcgGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGccc                                                                                         <  2:921155/150‑1 (MQ=255)
                                                                 ggcgCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGaaa                                                                                     <  1:1002875/150‑1 (MQ=255)
                                                                 ggcgCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGaaa                                                                                     <  2:153429/150‑1 (MQ=255)
                                                                   cgcATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACa                                                                                   <  2:1082010/150‑1 (MQ=255)
                                                                      aTCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAAt                                                                                <  1:502134/150‑1 (MQ=255)
                                                                      aTCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAAt                                                                                <  1:705174/150‑1 (MQ=255)
                                                                       tCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATg                                                                               >  1:81713/1‑150 (MQ=255)
                                                                          cgAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACCAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAg                                                                            >  2:1155816/1‑150 (MQ=255)
                                                                            aGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGCCCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGga                                                                          <  1:1420330/150‑1 (MQ=255)
                                                                               aaGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCTCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGga                                                                          >  2:469291/1‑147 (MQ=255)
                                                                               aaGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGga                                                                          <  1:469291/147‑1 (MQ=255)
                                                                                       tcCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCt                                                               <  1:285047/150‑1 (MQ=255)
                                                                                       tcCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCt                                                               >  1:1307233/1‑150 (MQ=255)
                                                                                             aTTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGAt                                                         >  2:427562/1‑150 (MQ=255)
                                                                                                  tttCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTcgcgcgc                                                                                                                 <  1:568039/89‑1 (MQ=2)
                                                                                                  tttCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTcgcgcgc                                                                                                                 >  2:568039/1‑89 (MQ=9)
                                                                                                  tttCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGccgaaa                                                                                      >  2:1208690/1‑112 (MQ=2)
                                                                                                  tttCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGccgaaa                                                                                      <  1:1208690/116‑5 (MQ=2)
                                                                                                   ttCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTaca                                                                                                                                   >  2:471854/1‑70 (MQ=255)
                                                                                                   ttCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTaca                                                                                                                                   <  1:471854/70‑1 (MQ=255)
                                                                                                      gggCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCcgcgc                                                >  1:644214/1‑150 (MQ=255)
                                                                                                         ccAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCgcggg                                             >  2:387601/1‑150 (MQ=255)
                                                                                                         ccAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTcgcgcgcgactg                                                                                                            >  1:806113/1‑83 (MQ=9)
                                                                                                                 tCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTc                                     >  1:1193423/1‑150 (MQ=255)
                                                                                                                   cTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGa                                   >  2:1265620/1‑150 (MQ=255)
                                                                                                                    tGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGAt                                  <  2:1307233/150‑1 (MQ=255)
                                                                                                                     gCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGAtc                                 <  1:427562/150‑1 (MQ=255)
                                                                                                                       gggATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGAtctc                               >  2:120440/1‑150 (MQ=255)
                                                                                                                          aTTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTcaca                            >  2:85996/1‑150 (MQ=255)
                                                                                                                                gcgcgACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGGGTTGGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAAc                      <  1:1155816/150‑1 (MQ=255)
                                                                                                                                      ccGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAg                                                                            >  2:361602/1‑90 (MQ=255)
                                                                                                                                      ccGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAg                                                                            <  1:361602/90‑1 (MQ=255)
                                                                                                                                       cGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGAGTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAAcc               >  1:353286/1‑150 (MQ=255)
                                                                                                                                       cGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTca                              <  2:24892/135‑1 (MQ=255)
                                                                                                                                       cGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTca                              >  1:24892/1‑135 (MQ=255)
                                                                                                                                          cTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCTCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTAt            >  1:212818/1‑150 (MQ=255)
                                                                                                                                          cTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGa                                                          <  1:1068064/104‑1 (MQ=255)
                                                                                                                                          cTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGa                                                          >  2:1068064/1‑104 (MQ=255)
                                                                                                                                            tCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCg          >  2:623023/1‑150 (MQ=255)
                                                                                                                                                 tACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGaa     <  2:1193423/150‑1 (MQ=255)
                                                                                                                                                    acCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAg  >  2:1212915/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAG  >  NC_002947/4945184‑4945481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑