Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,997,649 C→A 8.0% R167L (CGG→CTG)  PP_4405 ← sensory box protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,997,6490CA 8.0% 191.8 / 4.6 63R167L (CGG→CTG) PP_4405sensory box protein
Reads supporting (aligned to +/- strand):  ref base C (25/32);  new base A (2/3);  total (28/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

CCATGGCAATCAGCGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGATATTGCGATGCGCACCCAGCATGCGTGCCACGGAA  >  NC_002947/4997509‑4997795
                                                                                                                                            |                                                                                                                                                  
ccatgGCAATCAGCGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAAct                                                                                                                                           >  2:663867/1‑150 (MQ=255)
 catgGCAATCAGCGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAActg                                                                                                                                          >  1:1023965/1‑150 (MQ=255)
 catgGCAATCAGCGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAActg                                                                                                                                          >  1:1025827/1‑150 (MQ=255)
 catgGCAATCAGCGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAActg                                                                                                                                          >  2:828556/1‑150 (MQ=255)
   tgGCAATCAGCGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCAGCGGCAActgga                                                                                                                                        <  2:648556/150‑3 (MQ=255)
    ggCAATCAGCGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCTCTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAActgctg                                                                                                                                       <  2:1025827/150‑1 (MQ=255)
    ggCAATCAGCGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGGTCTCTAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAActgctg                                                                                                                                       <  1:871944/150‑1 (MQ=255)
    ggCAATCAGCGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAActgctg                                                                                                                                       <  1:181231/150‑1 (MQ=255)
    ggCAATCAGCGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAActgctg                                                                                                                                       <  2:1023965/150‑1 (MQ=255)
    ggCAATCAGCGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAActgctg                                                                                                                                       <  1:54967/150‑1 (MQ=255)
     gCAATCAGCGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTgg                                                                                                                                      <  1:1334428/150‑1 (MQ=255)
            gCGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCAccc                                                                                                                               <  2:789064/150‑1 (MQ=255)
            gCGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCAccc                                                                                                                               >  1:209497/1‑150 (MQ=255)
              gACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCAGGGTTCCCGTCCAGTTGCGGCTGCAACTGCTGGTTCACCCAg                                                                                                                             >  2:435727/1‑150 (MQ=255)
              gACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCAGCGGCAActgga                                                                                                                                        <  2:101943/139‑3 (MQ=255)
              gACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCAGCGGCAActgga                                                                                                                                        >  1:101943/1‑137 (MQ=255)
                cAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGa                                                                                                                           <  2:123193/150‑1 (MQ=255)
                cAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGa                                                                                                                           >  1:95732/1‑150 (MQ=255)
                           ggAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAActgc                                                                                                                                         <  1:527538/125‑1 (MQ=255)
                           ggAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAActgc                                                                                                                                         >  2:527538/1‑125 (MQ=255)
                            gAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACg                                                                                                                  >  2:817273/1‑147 (MQ=255)
                            gAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACg                                                                                                                  <  1:817273/147‑1 (MQ=255)
                                 gcTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGtt                                                                                                          >  2:1210943/1‑150 (MQ=255)
                                      cagcgcTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACCGGTGCGTTCGGcc                                                                                                     <  1:797131/148‑1 (MQ=255)
                                         cgcTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCAcc                                                                                                  <  2:209497/150‑1 (MQ=255)
                                          gcTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCt                                                                                                 >  1:1390159/1‑150 (MQ=255)
                                                    cAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAg                                                                                       <  1:938056/150‑1 (MQ=255)
                                                       cTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGa                                                                                    <  1:1210943/150‑1 (MQ=255)
                                                        tGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGAc                                                                                   <  1:159741/150‑1 (MQ=255)
                                                            acacgcTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGt                                                                               >  1:1421403/4‑150 (MQ=255)
                                                            aCGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCa                                                                                                                    >  2:843718/1‑113 (MQ=255)
                                                            aCGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCa                                                                                                                    <  1:843718/113‑1 (MQ=255)
                                                                      cAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTc                                                                     <  2:997506/150‑1 (MQ=255)
                                                                            gTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTg                                                               >  1:4229/1‑150 (MQ=255)
                                                                               aCGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTgaga                                                            <  2:1390159/150‑1 (MQ=255)
                                                                                cGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGac                                                           <  2:1075120/150‑1 (MQ=255)
                                                                                  gTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGacac                                                         <  1:828556/150‑1 (MQ=255)
                                                                                  gTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGacac                                                         <  2:1421403/150‑1 (MQ=255)
                                                                                        gACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTTAGACACCAAACg                                                   >  1:1353562/1‑150 (MQ=255)
                                                                                        gACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACg                                                   >  1:878219/1‑150 (MQ=255)
                                                                                                       aTCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGAc                                                                                   <  2:786899/103‑1 (MQ=255)
                                                                                                       aTCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGAc                                                                                   >  1:786899/1‑103 (MQ=255)
                                                                                                          gcgTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCa                                            <  2:589134/139‑1 (MQ=255)
                                                                                                          gcgTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCa                                            >  1:589134/1‑139 (MQ=255)
                                                                                                          gcgTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGATAtt                                 >  2:713082/1‑150 (MQ=255)
                                                                                                                ggCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGATATTGCGATg                           >  2:714339/1‑150 (MQ=255)
                                                                                                                 gCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGAc                                                                                   >  2:866121/1‑93 (MQ=255)
                                                                                                                 gCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGAc                                                                                   <  1:866121/93‑1 (MQ=255)
                                                                                                                 gCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGATATTGCGATgc                          >  1:702026/1‑150 (MQ=255)
                                                                                                                      ttCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGATATTGCGATGCGCAcc                     >  2:1109533/1‑150 (MQ=255)
                                                                                                                        cACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGATATTGCGATGCGCACCCa                   <  2:1353562/150‑1 (MQ=255)
                                                                                                                         aCGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGATATTGCGATGCGCACCCAg                  <  2:95732/150‑1 (MQ=255)
                                                                                                                                tcGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGATATTGCGATGCGCCCCCAGCATTCGt           <  2:878219/150‑1 (MQ=255)
                                                                                                                                tcGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGATATTGCGATGCGCACCCAGCATGCGt           <  1:663867/150‑1 (MQ=255)
                                                                                                                                  cagcAGTTGCAGCGGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGACAGCGTCGATATTGCGAGGCGCACCCAGCAGGCGtac         >  2:1373941/4‑148 (MQ=255)
                                                                                                                                   tCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCtt                                                <  1:916011/110‑1 (MQ=255)
                                                                                                                                   tCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCtt                                                >  2:916011/1‑110 (MQ=255)
                                                                                                                                     cAGTTGCAGCGGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGATATTGCGATGCGCACCCAGCATGCGTGCCAc      <  1:1373941/150‑1 (MQ=255)
                                                                                                                                       gTTGCCGCTGCAAATGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGATATTGCGATGCGCACCCAGCAt               <  2:612828/139‑1 (MQ=255)
                                                                                                                                       gTTGCCGCTGCAAATGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGATATTGCGATGCGCACCCAGCAt               >  1:612828/1‑139 (MQ=255)
                                                                                                                                        ttGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGaca                                                          <  2:674521/95‑1 (MQ=255)
                                                                                                                                        ttGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGaca                                                          >  1:674521/1‑95 (MQ=255)
                                                                                                                                         tGCCGCTGCAACTGCTGGTTCCCCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGATATTGCGATGCGCACCACGCATGAGTGCCACggca  <  2:733151/150‑3 (MQ=255)
                                                                                                                                            |                                                                                                                                                  
CCATGGCAATCAGCGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCAATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGATATTGCGATGCGCACCCAGCATGCGTGCCACGGAA  >  NC_002947/4997509‑4997795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑